FastQCFastQC Report
Sat 14 Jan 2017
SRR2934880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432568
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT894163.675786247290929No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC35230.1448263727879344TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC34360.14124990544971405TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA27280.11214486090419672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC207.034842E-445.033
CGTTTTT695400.044.5017241
AATACGG750.042.0000042
GTGTCGA501.0822987E-940.49999611
GTTTTTT782600.039.732942
TAGTCCG403.4603545E-739.3751
TACGGGT1900.039.0789454
CAACGAC1450.038.79310212
CGTTCGA356.251168E-638.57142614
AATAGCG356.251168E-638.5714261
CTTACGA356.251168E-638.57142629
TACGGGA6050.038.3057824
CGGTCTA900.037.531
TCGGTAC301.1402968E-437.49999636
CGATGAT800.036.562510
CGCAATC1600.036.562520
ATAACGG1050.036.4285742
GATTACG1300.036.3461571
ATATCGA250.002107570635.99999621
ACGTCGA250.002107570635.99999622
Produced by FastQC (version 0.11.5)