##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934880.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2432568
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70120547503708	34.0	31.0	34.0	31.0	34.0
2	32.84945909014671	34.0	31.0	34.0	31.0	34.0
3	32.89266610429801	34.0	31.0	34.0	31.0	34.0
4	36.26876617632066	37.0	37.0	37.0	35.0	37.0
5	36.16991755215065	37.0	37.0	37.0	35.0	37.0
6	36.16507000009866	37.0	37.0	37.0	35.0	37.0
7	36.30190810698817	37.0	37.0	37.0	35.0	37.0
8	36.31377457896347	37.0	37.0	37.0	35.0	37.0
9	38.08773814339414	39.0	39.0	39.0	37.0	39.0
10	37.667489665242655	39.0	38.0	39.0	35.0	39.0
11	37.58886699159078	39.0	37.0	39.0	35.0	39.0
12	37.56618643343167	39.0	37.0	39.0	35.0	39.0
13	37.575573632474	39.0	37.0	39.0	35.0	39.0
14	38.93995727971428	40.0	38.0	41.0	35.0	41.0
15	38.98941283450247	40.0	38.0	41.0	35.0	41.0
16	39.06526600695232	41.0	38.0	41.0	35.0	41.0
17	39.06821022063926	41.0	38.0	41.0	35.0	41.0
18	38.527991817700475	39.0	38.0	41.0	35.0	41.0
19	37.99325363155316	38.0	37.0	41.0	35.0	41.0
20	37.39463233915763	38.0	35.0	41.0	35.0	41.0
21	37.354977127052564	38.0	35.0	41.0	35.0	41.0
22	37.170984737117315	38.0	35.0	41.0	34.0	41.0
23	37.058618299673434	38.0	35.0	40.0	34.0	41.0
24	36.85981563516415	38.0	35.0	40.0	33.0	41.0
25	36.73589227515942	37.0	35.0	40.0	33.0	41.0
26	36.84090311144437	38.0	35.0	40.0	34.0	41.0
27	36.86296045989259	38.0	35.0	40.0	34.0	41.0
28	36.78583908034636	38.0	35.0	40.0	33.0	41.0
29	36.6392655827093	38.0	35.0	40.0	33.0	41.0
30	36.33037103176561	38.0	35.0	40.0	33.0	41.0
31	35.772333188630284	38.0	35.0	41.0	31.0	41.0
32	34.9700616796735	38.0	35.0	41.0	22.0	41.0
33	33.92526046548339	38.0	34.0	41.0	15.0	41.0
34	33.04918423657632	38.0	33.0	41.0	10.0	41.0
35	32.52016798708196	38.0	32.0	41.0	8.0	41.0
36	32.26479218669324	38.0	31.0	41.0	8.0	41.0
37	32.003441219320486	38.0	30.0	41.0	7.0	41.0
38	31.854019702635238	37.0	29.0	41.0	7.0	41.0
39	31.764432895606618	37.0	29.0	41.0	7.0	41.0
40	31.624632487149384	37.0	28.0	40.0	7.0	41.0
41	31.480652956053028	37.0	27.0	40.0	7.0	41.0
42	31.351297065487994	37.0	25.0	40.0	7.0	41.0
43	31.249607410769194	37.0	24.0	40.0	7.0	41.0
44	31.135463427949393	37.0	24.0	40.0	7.0	41.0
45	31.082842905110976	36.0	23.0	40.0	7.0	41.0
46	31.025188196177865	36.0	23.0	40.0	7.0	41.0
47	31.002237553071488	36.0	23.0	40.0	7.0	41.0
48	30.933853442123713	36.0	23.0	40.0	7.0	41.0
49	30.799700974443468	36.0	24.0	40.0	7.0	41.0
50	30.701054605667757	35.0	24.0	40.0	7.0	41.0
51	30.14280176340394	35.0	23.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	5.0
9	15.0
10	14.0
11	21.0
12	25.0
13	33.0
14	45.0
15	90.0
16	195.0
17	392.0
18	723.0
19	1454.0
20	2701.0
21	4292.0
22	6957.0
23	12432.0
24	25735.0
25	57268.0
26	104696.0
27	134241.0
28	123339.0
29	95033.0
30	71011.0
31	59788.0
32	55847.0
33	64916.0
34	109736.0
35	143536.0
36	155555.0
37	209422.0
38	402054.0
39	590612.0
40	380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.120994767669394	15.373177646010308	17.515440472784316	37.99038711353598
2	52.71120889529091	17.46935748558725	17.55757701326335	12.2618566058585
3	23.147801007001654	17.82712754586922	46.7757119225444	12.249359524584719
4	20.665938218376628	19.378985500097016	46.57078445494638	13.384291826579977
5	18.794500297627856	22.18182595512232	45.14032084611818	13.883352901131643
6	19.69630448151912	24.53625140181076	44.955413373850185	10.812030742819934
7	58.63387991620378	4.704082270259248	33.28079626139947	3.381241552137494
8	59.437721782083784	4.218957085680647	33.182833943388225	3.160487188847341
9	54.70708321411776	5.964232037912199	34.68145597574251	4.647228772227539
10	29.33204744944437	19.15765561332715	40.533666479210446	10.976630458018029
11	22.836566130936525	19.20094320076561	44.55390352910998	13.408587139187885
12	20.898244160081035	17.55235619312595	47.40007268039372	14.149326966399295
13	18.70410200249284	17.800859009902293	48.67674819367845	14.818290793926419
14	15.13306102850979	20.369666952784055	48.168314308171446	16.328957710534713
15	14.924844855313395	19.583830750055085	50.77671004469351	14.714614349938007
16	16.996030532342775	18.637916802325773	49.71026503678417	14.655787628547278
17	16.814247330393233	19.031163774250093	47.75841826415541	16.396170631201265
18	17.102173505529958	19.54740833555321	47.94377793344318	15.406640225473655
19	17.982601103031858	20.14171854599748	47.00719568784922	14.868484663121443
20	18.913633657928575	19.743209645115776	47.61215308266819	13.731003614287452
21	18.55142384508881	19.435140148189074	47.48849775216972	14.524938254552392
22	17.407406493878074	18.937887861716508	47.21409637880626	16.44060926559915
23	16.966062202577685	19.409734897441716	47.98303685652364	15.641166043456955
24	16.711845259824184	18.724861956582508	48.36206017673504	16.20123260685827
25	16.567841063435843	19.68993261442229	47.184333593141076	16.55789272900079
26	16.11778170229979	20.60193178566848	47.689314337769794	15.590972174261934
27	16.708885424785656	20.782851702398453	47.27645845871524	15.231804414100655
28	15.016599741507742	21.197393043072175	48.123629020853684	15.6623781945664
29	16.052418678532316	19.70711610117374	47.750936458919135	16.48952876137481
30	17.113683975124232	20.074505625330925	46.51072446895627	16.30108593058858
31	17.22015581887125	21.337450792742484	44.94086085157743	16.50153253680884
32	17.732371715816374	22.203613629711484	43.673229278688204	16.390785375783945
33	18.211741665597838	23.430218600261124	41.277695012020224	17.08034472212082
34	17.450652972496556	24.603217669557438	40.170223401771295	17.77590595617471
35	18.691399377119158	24.903928687707804	37.39328150333311	19.011390431839935
36	19.256727869477853	26.81758536657557	35.92565552124339	18.000031242703184
37	19.07552019100802	27.410086788940742	36.186984289853356	17.32740873019788
38	18.89793008869639	27.129231330840494	34.64573241118028	19.327106169282832
39	19.606070621664017	25.462556442409834	35.53170969937942	19.399663236546726
40	20.1776476546596	25.412198137934887	36.6682041365339	17.741950070871606
41	18.585297512751957	26.77158459701846	35.40793104242101	19.23518684780857
42	19.061913171594792	26.810966846558866	34.52203597186184	19.605084009984513
43	20.008032663424004	26.5532556541071	34.19263099736575	19.246080685103152
44	19.001894294424655	27.127052563381575	34.057629632552924	19.81342350964084
45	18.89608019179731	25.88190751502116	34.67212427360715	20.549888019574375
46	19.2126181056398	26.18915483554828	35.09903936909472	19.499187689717203
47	18.314020409706945	25.815968967773973	36.60608870954481	19.263921912974272
48	18.42452091781196	24.832687102683256	36.45949465749776	20.283297322007034
49	18.64560415166195	24.157926931539013	37.16389428784725	20.032574628951792
50	17.982148906012082	23.919701319757554	37.35024056881452	20.74790920541584
51	17.533610571215274	23.66786868856287	36.293415024780394	22.50510571544146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	537.0
1	1218.5
2	1900.0
3	96633.0
4	191366.0
5	135550.0
6	79734.0
7	76030.0
8	72326.0
9	70058.5
10	67791.0
11	64528.5
12	61266.0
13	57453.5
14	53641.0
15	49591.5
16	45542.0
17	41978.0
18	38414.0
19	35386.5
20	32359.0
21	29556.5
22	26754.0
23	25132.0
24	23510.0
25	22579.5
26	23494.0
27	25339.0
28	27078.0
29	28817.0
30	33350.0
31	37883.0
32	40447.0
33	43011.0
34	47880.5
35	52750.0
36	56670.5
37	60591.0
38	64077.0
39	67563.0
40	75331.5
41	83100.0
42	90329.0
43	97558.0
44	103664.5
45	109771.0
46	115444.0
47	121117.0
48	131867.0
49	142617.0
50	149981.5
51	157346.0
52	152809.5
53	148273.0
54	131213.0
55	114153.0
56	103767.5
57	93382.0
58	87621.5
59	81861.0
60	78050.5
61	74240.0
62	66487.0
63	58734.0
64	50749.0
65	42764.0
66	34151.0
67	25538.0
68	21511.0
69	17484.0
70	15125.5
71	12767.0
72	10296.5
73	7826.0
74	6499.0
75	3996.5
76	2821.0
77	2271.0
78	1721.0
79	1216.0
80	711.0
81	592.5
82	474.0
83	346.5
84	219.0
85	165.0
86	111.0
87	79.0
88	47.0
89	27.0
90	7.0
91	7.5
92	8.0
93	4.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2432568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.372007247167737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21597047447149	23.755760220949057
2	9.212282885884214	5.595910451460693
3	3.5431579407635105	3.2283845596418774
4	1.7425217534505808	2.1169553329659387
5	1.0507893060064706	1.595729020863744
6	0.696731584339986	1.269668203734283
7	0.4648778541196632	0.9883491488059136
8	0.3467890145220761	0.8426142769842122
9	0.26346047685462176	0.7201641161133755
>10	3.2101380661829797	24.28773257238226
>50	1.1274644258989934	23.13960320735615
>100	0.11817883758363493	6.307566191824611
>500	0.004721289406137459	0.9461963629375805
>1k	0.002777229062433799	1.434245585666071
>5k	0.0	0.0
>10k+	1.3886145312168996E-4	3.771120748314094
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89416	3.675786247290929	No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	3523	0.1448263727879344	TruSeq Adapter, Index 22 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	3436	0.14124990544971405	TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2728	0.11214486090419672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2332645993863274E-4	0.0	0.0	0.08752067773644971	0.0
2	1.2332645993863274E-4	0.0	0.0	0.49137372521549244	0.0
3	1.2332645993863274E-4	0.0	0.0	0.7225286199604698	0.0
4	1.2332645993863274E-4	0.0	0.0	0.9758411686744214	0.0
5	1.2332645993863274E-4	0.0	0.0	1.614220034136764	0.0
6	1.2332645993863274E-4	0.0	0.0	1.9719489855987582	0.0
7	1.2332645993863274E-4	0.0	0.0	2.3277458225217136	0.0
8	1.2332645993863274E-4	0.0	0.0	2.899775052537072	0.0
9	1.2332645993863274E-4	0.0	0.0	3.1003038763972888	0.0
10	1.2332645993863274E-4	0.0	0.0	3.6012148478480355	0.0
11	1.2332645993863274E-4	0.0	0.0	4.365098940707926	0.0
12	1.2332645993863274E-4	0.0	0.0	4.998256986032867	0.0
13	1.2332645993863274E-4	0.0	0.0	5.2145715967652295	0.0
14	1.2332645993863274E-4	0.0	0.0	5.309532970917976	0.0
15	1.2332645993863274E-4	0.0	0.0	5.49234389336701	0.0
16	1.2332645993863274E-4	0.0	0.0	5.90811849864012	0.0
17	1.2332645993863274E-4	0.0	0.0	6.397683435776512	0.0
18	1.2332645993863274E-4	0.0	0.0	6.963957430994735	0.0
19	1.64435279918177E-4	0.0	0.0	7.296938872829044	0.0
20	1.64435279918177E-4	0.0	0.0	7.636579943500037	0.0
21	2.0554409989772125E-4	0.0	0.0	8.067729247445499	0.0
22	2.0554409989772125E-4	0.0	0.0	8.539699609630645	0.0
23	2.0554409989772125E-4	0.0	0.0	9.000570590421317	0.0
24	2.0554409989772125E-4	0.0	0.0	9.367425699918769	0.0
25	2.0554409989772125E-4	0.0	0.0	9.70011938001322	0.0
26	2.0554409989772125E-4	0.0	0.0	10.005599021281213	0.0
27	2.0554409989772125E-4	0.0	0.0	10.311654186028921	0.0
28	2.0554409989772125E-4	0.0	0.0	10.634111769948467	0.0
29	2.0554409989772125E-4	0.0	0.0	10.9594058624466	0.0
30	2.0554409989772125E-4	0.0	0.0	11.34360889397542	0.0
31	2.0554409989772125E-4	0.0	0.0	11.715027082490602	0.0
32	2.0554409989772125E-4	0.0	0.0	12.08052560092873	0.0
33	2.0554409989772125E-4	0.0	0.0	12.42065997743948	0.0
34	2.466529198772655E-4	0.0	0.0	12.7598488510907	0.0
35	2.466529198772655E-4	0.0	0.0	13.099448812941715	0.0
36	2.466529198772655E-4	0.0	0.0	13.459644293602482	0.0
37	2.466529198772655E-4	0.0	0.0	13.831638005597377	0.0
38	2.466529198772655E-4	0.0	0.0	14.180323016663872	0.0
39	2.466529198772655E-4	0.0	0.0	14.530200183509772	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTC	20	7.034842E-4	45.0	33
CGTTTTT	69540	0.0	44.501724	1
AATACGG	75	0.0	42.000004	2
GTGTCGA	50	1.0822987E-9	40.499996	11
GTTTTTT	78260	0.0	39.73294	2
TAGTCCG	40	3.4603545E-7	39.375	1
TACGGGT	190	0.0	39.078945	4
CAACGAC	145	0.0	38.793102	12
CGTTCGA	35	6.251168E-6	38.571426	14
AATAGCG	35	6.251168E-6	38.571426	1
CTTACGA	35	6.251168E-6	38.571426	29
TACGGGA	605	0.0	38.305782	4
CGGTCTA	90	0.0	37.5	31
TCGGTAC	30	1.1402968E-4	37.499996	36
CGATGAT	80	0.0	36.5625	10
CGCAATC	160	0.0	36.5625	20
ATAACGG	105	0.0	36.428574	2
GATTACG	130	0.0	36.346157	1
ATATCGA	25	0.0021075706	35.999996	21
ACGTCGA	25	0.0021075706	35.999996	22
>>END_MODULE