FastQCFastQC Report
Sat 14 Jan 2017
SRR2934879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388394
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543753.9163954900410114No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC32440.23365125461504446No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC29640.213484068643339No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC27640.19907893580640657No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG25700.18510595695458207No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC21980.1583124098778877No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT17250.12424427071854242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8912258E-545.0000041
GACCCGT253.8912258E-545.00000414
CGTAGAT207.033795E-445.012
CGATATT351.2121745E-745.011
ACGACTA207.033795E-445.037
TGCGTCG207.033795E-445.01
ATCGACG302.1658307E-644.9999961
CCCGATA302.1658307E-644.99999613
CGTTTTT383000.044.5946461
GCTTACG650.041.5384641
TAACGCG603.6379788E-1241.2499961
TAGTGCG1400.040.178571
TACGTAG451.9288564E-840.01
GTTTTTT427900.039.9994162
CGAATAT800.039.37514
TATAGCG800.039.3751
GCGTACT403.4589175E-739.37518
TAGTACG403.4589175E-739.3751
ACGGTCC356.249227E-638.57142640
CTTACGG1000.038.2500042
Produced by FastQC (version 0.11.5)