##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934878.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742735
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.331496428739726	33.0	31.0	34.0	30.0	34.0
2	32.52991578422991	34.0	31.0	34.0	31.0	34.0
3	32.58647431452672	34.0	31.0	34.0	31.0	34.0
4	36.05134267268945	37.0	35.0	37.0	35.0	37.0
5	35.901256841269095	37.0	35.0	37.0	35.0	37.0
6	35.88745784162588	37.0	35.0	37.0	35.0	37.0
7	36.09398641507401	37.0	35.0	37.0	35.0	37.0
8	36.10710280248002	37.0	36.0	37.0	35.0	37.0
9	37.95607585477997	39.0	39.0	39.0	35.0	39.0
10	37.40168835452752	39.0	37.0	39.0	34.0	39.0
11	37.32627922475715	39.0	37.0	39.0	34.0	39.0
12	37.33050684295206	39.0	37.0	39.0	34.0	39.0
13	37.28666348024531	39.0	37.0	39.0	34.0	39.0
14	38.436038425548816	40.0	38.0	41.0	34.0	41.0
15	38.48454428564697	40.0	38.0	41.0	34.0	41.0
16	38.586883612593994	40.0	38.0	41.0	34.0	41.0
17	38.62924730893253	40.0	38.0	41.0	34.0	41.0
18	38.191488215850875	39.0	38.0	40.0	35.0	41.0
19	37.71422916652642	38.0	37.0	40.0	34.0	41.0
20	37.157804600564134	38.0	35.0	40.0	34.0	41.0
21	37.09570438985641	38.0	35.0	40.0	34.0	41.0
22	36.89773876281581	38.0	35.0	40.0	33.0	41.0
23	36.75686617703488	38.0	35.0	40.0	33.0	41.0
24	36.53715524379489	37.0	35.0	40.0	33.0	41.0
25	36.4158643392327	37.0	35.0	40.0	33.0	41.0
26	36.60346018431877	37.0	35.0	40.0	33.0	41.0
27	36.6845779450275	38.0	35.0	40.0	33.0	41.0
28	36.6879896598383	38.0	35.0	40.0	33.0	41.0
29	36.63121436313086	38.0	35.0	40.0	33.0	41.0
30	36.363655947275944	38.0	35.0	40.0	33.0	41.0
31	35.84417053188553	38.0	35.0	40.0	31.0	41.0
32	35.219651692730245	38.0	35.0	40.0	25.0	41.0
33	34.3166162897938	38.0	35.0	40.0	18.0	41.0
34	33.55893420937481	38.0	34.0	40.0	15.0	41.0
35	33.09389890068463	38.0	33.0	40.0	10.0	41.0
36	32.85130497418326	38.0	33.0	40.0	10.0	41.0
37	32.62304321191273	37.0	33.0	40.0	9.0	41.0
38	32.531917844183994	37.0	32.0	40.0	8.0	41.0
39	32.46728914081065	37.0	32.0	40.0	8.0	41.0
40	32.33561095141605	37.0	32.0	40.0	8.0	41.0
41	32.18599635132315	37.0	31.0	40.0	8.0	41.0
42	32.0588325580456	37.0	31.0	40.0	8.0	41.0
43	31.955927753505623	37.0	31.0	40.0	8.0	41.0
44	31.83900179741092	37.0	30.0	40.0	7.0	41.0
45	31.790113566749916	37.0	30.0	40.0	7.0	41.0
46	31.662145987465248	37.0	30.0	40.0	7.0	41.0
47	31.576259365722635	36.0	30.0	40.0	7.0	41.0
48	31.518665472880638	36.0	29.0	40.0	7.0	41.0
49	31.392312197486316	36.0	28.0	40.0	7.0	41.0
50	31.2686503261594	36.0	28.0	40.0	7.0	41.0
51	30.626853453789035	35.0	26.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	8.0
11	7.0
12	9.0
13	13.0
14	12.0
15	32.0
16	56.0
17	112.0
18	269.0
19	500.0
20	905.0
21	1457.0
22	2535.0
23	4401.0
24	8926.0
25	19160.0
26	31333.0
27	34252.0
28	27712.0
29	20472.0
30	17013.0
31	16245.0
32	17983.0
33	22710.0
34	38170.0
35	57733.0
36	69155.0
37	78466.0
38	139300.0
39	133717.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.61002914902354	17.80769722714023	20.441072522501297	35.14120110133493
2	47.95855857068806	19.36074104492181	19.226238160312896	13.45446222407723
3	21.956552471608312	17.99740149582287	47.16742849064606	12.878617541922758
4	20.912303849959947	19.1928480548244	46.27222360599676	13.622624489218902
5	17.47568109756508	21.65779180999953	46.29834328529018	14.568183807145212
6	18.15008044591947	23.216086491144218	45.59001528135877	13.043817781577548
7	56.461052730785546	4.354850653328576	33.73558537028685	5.448511245599036
8	56.16296525678741	3.272230337872862	33.524069823018976	7.040734582320747
9	52.98848175998169	4.273260314917164	35.899075713410575	6.839182211690575
10	26.428672406713027	17.804735201653347	42.19997711162124	13.566615280012387
11	18.927881411270505	20.28516227187355	44.849912822204416	15.937043494651522
12	17.368778904993032	17.829104593159066	49.93086363238571	14.87125286946219
13	15.97151070031707	20.751277373491217	50.30744478178624	12.969767144405475
14	12.533406935178764	23.868270648346986	47.53741240146216	16.060910015012084
15	10.798063912431756	21.29911745104243	52.38759449871084	15.515224137814968
16	11.607235420439324	21.92800931691653	49.14511905322894	17.3196362094152
17	11.793843026112947	21.481820568574257	46.02502911536416	20.699307289948635
18	13.107905242111926	20.872181868364894	49.36498212686894	16.654930762654242
19	13.08542077591604	22.35629127481538	46.65338243114974	17.904905518118845
20	13.768436925686819	23.720977199135625	46.67667472247841	15.833911152699146
21	13.633260853467252	23.44375854106781	47.720923344126774	15.202057261338162
22	13.023352878213629	22.518933401549678	44.63099221121934	19.826721509017347
23	11.618410334776199	22.056318875507415	46.48306596565397	19.842204824062417
24	14.868425481497438	20.498966657017643	46.33886917945162	18.293738682033293
25	12.295771708617474	22.569961022437344	45.26850087850983	19.86576639043535
26	11.38346785865753	22.980874739981285	47.52408328677119	18.111574114589995
27	13.934444990474395	21.645943708051995	46.18120864103617	18.238402660437437
28	12.519135357832875	20.462479888520132	46.65109359327351	20.367291160373487
29	12.328084713928925	18.689707634620692	48.094946380606814	20.88726127084357
30	14.78481558025406	20.555918328879073	45.05456185584361	19.60470423502326
31	15.78019078136886	24.198132577568042	41.312985115821924	18.708691525241168
32	15.797155109157371	22.375544440480116	41.24580099227854	20.581499458083975
33	15.61256706631571	21.255629531394106	42.30674466667115	20.825058735619027
34	15.22386853992339	22.327748120123598	41.63557661884791	20.812806721105105
35	15.327539431964293	21.989538664530418	39.23377786155224	23.44914404195305
36	17.686927369788684	22.395874706321905	40.23763522656129	19.67956269732812
37	17.78561667351074	24.745972655119257	38.27462015389069	19.193790517479318
38	17.95727951422782	24.747722942906957	35.409533682942104	21.88546385992312
39	17.427480864642167	23.184715948487682	39.89309780742795	19.494705379442195
40	18.91939924737625	23.604515742492275	38.79324388913946	18.68284112099201
41	18.168795061495686	23.90610379206581	35.225349552666835	22.69975159377167
42	19.277265781200562	21.63342241849381	37.74051310359684	21.348798696708783
43	20.110133493103195	21.35337637246124	36.880313974701615	21.656176159733956
44	17.882690326967225	22.386988629861257	35.35433229886837	24.37598874430315
45	17.732030939702586	21.558294681144687	34.779295441846685	25.93037893730604
46	19.831972372380456	22.247504156933495	36.97913791594579	20.94138555474025
47	16.126882400856292	22.73368024934869	39.768827374501	21.370609975294013
48	16.892027439127013	20.680592674372424	38.240826135835796	24.18655375066477
49	18.607444108598624	18.523430294788852	40.74656506021663	22.122560536395888
50	17.758958444128794	18.698862986125604	38.86352467569187	24.678653894053735
51	16.184103347761987	19.5042646435135	34.727998545914765	29.58363346280975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	602.0
1	617.0
2	632.0
3	27275.0
4	53918.0
5	34281.5
6	14645.0
7	14509.0
8	14373.0
9	15308.0
10	16243.0
11	16713.5
12	17184.0
13	17180.5
14	17177.0
15	16391.0
16	15605.0
17	14716.0
18	13827.0
19	12578.5
20	11330.0
21	10374.0
22	9418.0
23	8804.5
24	8191.0
25	7724.5
26	7297.5
27	7337.0
28	7040.5
29	6744.0
30	7404.0
31	8064.0
32	8745.5
33	9427.0
34	10225.0
35	11023.0
36	12637.5
37	14252.0
38	15490.5
39	16729.0
40	20175.5
41	23622.0
42	28793.5
43	33965.0
44	40678.5
45	47392.0
46	58344.0
47	69296.0
48	80692.0
49	92088.0
50	88813.0
51	85538.0
52	65120.0
53	44702.0
54	35280.5
55	25859.0
56	20820.0
57	15781.0
58	13324.5
59	10868.0
60	9032.5
61	7197.0
62	5605.0
63	4013.0
64	3722.5
65	3432.0
66	2409.0
67	1386.0
68	1294.5
69	1203.0
70	1029.0
71	855.0
72	726.0
73	597.0
74	492.5
75	266.5
76	145.0
77	179.0
78	213.0
79	158.5
80	104.0
81	86.0
82	68.0
83	48.0
84	28.0
85	20.0
86	12.0
87	7.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.81381107340656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96538620282567	29.096923371214338
2	11.151360722839057	8.656536166153632
3	4.5610250489950825	5.3109229365831006
4	2.336954482945572	3.6282443915279976
5	1.40287239289063	2.7225412008877345
6	0.9093033079121624	2.117611608103578
7	0.6304010403654319	1.712778681685597
8	0.4958168607340621	1.5395633567633136
9	0.35003479444565044	1.2227565942658958
>10	2.9292085056704464	24.385666739014386
>50	0.19666703231264343	4.875369793608146
>100	0.06249562501124314	4.485477084528861
>500	0.004943156780550292	1.2186025736520185
>1k	0.002471578390275146	1.915612547740431
>5k	7.061652543643274E-4	1.6415229000454203
>10k+	3.530826271821637E-4	5.469870054225485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39913	5.373787420816307	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	6732	0.9063797989861795	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	5246	0.70630844109945	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	3657	0.4923694184332232	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	2461	0.3313429419644961	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	2389	0.321649040371061	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	1851	0.24921405346456002	No Hit
ACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1576	0.21218873487852333	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	1042	0.1402922980605465	No Hit
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	1002	0.1349067971753048	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	908	0.12225087009498678	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG	853	0.11484580637777943	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	837	0.11269160602368274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5194315603815627	0.0
2	0.0	0.0	0.0	2.8164823254592823	0.0
3	0.0	0.0	0.0	3.9991383198583614	0.0
4	0.0	0.0	0.0	5.17142722505335	0.0
5	0.0	0.0	0.0	8.037186883612595	0.0
6	0.0	0.0	0.0	9.522508027762257	0.0
7	0.0	0.0	0.0	11.117020202360196	0.0
8	0.0	0.0	0.0	13.48825624213212	0.0
9	0.0	0.0	0.0	14.314526715450329	0.0
10	0.0	0.0	0.0	16.17292843342511	0.0
11	0.0	0.0	0.0	19.194598342612103	0.0
12	0.0	0.0	0.0	21.26895864608508	0.0
13	0.0	0.0	0.0	22.048913811790207	0.0
14	1.346375221310427E-4	0.0	0.0	22.313341905255577	0.0
15	1.346375221310427E-4	0.0	0.0	22.810558274485516	0.0
16	1.346375221310427E-4	0.0	0.0	24.085037058977967	0.0
17	1.346375221310427E-4	0.0	0.0	25.687492847381638	0.0
18	1.346375221310427E-4	0.0	0.0	27.414757618800785	0.0
19	1.346375221310427E-4	0.0	0.0	28.351161585222187	0.0
20	1.346375221310427E-4	0.0	0.0	29.1752778581863	0.0
21	1.346375221310427E-4	0.0	0.0	30.26139874921742	0.0
22	1.346375221310427E-4	0.0	0.0	31.309147946441193	0.0
23	1.346375221310427E-4	0.0	0.0	32.285808531979775	0.0
24	1.346375221310427E-4	0.0	0.0	33.08151628777424	0.0
25	1.346375221310427E-4	0.0	0.0	33.711889166391785	0.0
26	1.346375221310427E-4	0.0	0.0	34.28652211084707	0.0
27	1.346375221310427E-4	0.0	0.0	34.85711592963843	0.0
28	1.346375221310427E-4	0.0	0.0	35.38920341710031	0.0
29	1.346375221310427E-4	0.0	0.0	35.98860966562771	0.0
30	1.346375221310427E-4	0.0	0.0	36.630830646192784	0.0
31	2.692750442620854E-4	0.0	0.0	37.20909880374562	0.0
32	2.692750442620854E-4	0.0	0.0	37.72543370111816	0.0
33	2.692750442620854E-4	0.0	0.0	38.25684800096939	0.0
34	2.692750442620854E-4	0.0	0.0	38.75850740842966	0.0
35	2.692750442620854E-4	0.0	0.0	39.264609854120245	0.0
36	2.692750442620854E-4	0.0	0.0	39.725878004941194	0.0
37	2.692750442620854E-4	0.0	0.0	40.18270311753182	0.0
38	2.692750442620854E-4	0.0	0.0	40.621890714723285	0.0
39	2.692750442620854E-4	0.0	0.0	41.09743044289013	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	35	1.2111559E-7	45.000004	1
CGGTCTA	65	0.0	45.000004	31
TATCCGG	35	1.2111559E-7	45.000004	2
AACGTAG	20	7.031672E-4	45.0	1
TTGGCTA	20	7.031672E-4	45.0	34
TCGTTGT	25	3.8894676E-5	45.0	23
GGTCGTG	20	7.031672E-4	45.0	8
CATGCGT	20	7.031672E-4	45.0	29
CTATCGC	20	7.031672E-4	45.0	20
GTCCTCG	20	7.031672E-4	45.0	16
CGTTGTA	25	3.8894676E-5	45.0	24
ATTCGGT	25	3.8894676E-5	45.0	15
CGCTGAT	20	7.031672E-4	45.0	7
GCCGATC	25	3.8894676E-5	45.0	9
GTAGCGA	45	3.8380676E-10	45.0	34
CGCTCCA	20	7.031672E-4	45.0	15
CTACGTA	25	3.8894676E-5	45.0	29
TACGAAG	20	7.031672E-4	45.0	1
AGCGCTA	25	3.8894676E-5	45.0	10
ACGTGAG	25	3.8894676E-5	45.0	1
>>END_MODULE