FastQCFastQC Report
Sat 14 Jan 2017
SRR2934876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208464
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT893804.047156756913402No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA24950.11297444739873505No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC24920.11283860637981873No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC24650.1116160372095719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTA302.1664146E-645.00000435
CGACATA207.0347014E-445.032
TTACGCG207.0347014E-445.01
CGTTTTT627800.044.491081
CGCATCG501.0822987E-940.50000421
GTATACG451.9297659E-840.01
GTTTTTT703800.039.833762
GTCTCGA403.4601544E-739.37527
TATAGCG750.039.01
GTTAGCG1000.038.2500041
TCGACGT601.5643309E-1037.50000426
CGAACTA250.002107527836.00000412
CGTAACG250.002107527836.0000041
TATCGCG504.8825314E-836.0000041
TGCGTCG250.002107527836.0000042
CCGATAC702.1827873E-1135.35714311
GCGTTAG2100.035.3571431
AATAGCG702.1827873E-1135.3571431
TCGATCA1150.035.2173917
CAATACG458.7047556E-735.01
Produced by FastQC (version 0.11.5)