##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934876.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2208464
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71300007607097	34.0	31.0	34.0	31.0	34.0
2	32.86418705489426	34.0	31.0	34.0	31.0	34.0
3	32.905759387520014	34.0	33.0	34.0	31.0	34.0
4	36.26908521035435	37.0	37.0	37.0	35.0	37.0
5	36.17497138282535	37.0	37.0	37.0	35.0	37.0
6	36.1819594976418	37.0	37.0	37.0	35.0	37.0
7	36.31725217164509	37.0	37.0	37.0	35.0	37.0
8	36.334078798658254	37.0	37.0	37.0	35.0	37.0
9	38.121373950401725	39.0	39.0	39.0	37.0	39.0
10	37.734960135188985	39.0	38.0	39.0	35.0	39.0
11	37.57481444116816	39.0	37.0	39.0	35.0	39.0
12	37.58914023502307	39.0	37.0	39.0	35.0	39.0
13	37.61988151040723	39.0	37.0	39.0	35.0	39.0
14	38.992388827710116	40.0	38.0	41.0	35.0	41.0
15	39.043386263031685	41.0	39.0	41.0	35.0	41.0
16	39.103854986995486	41.0	39.0	41.0	35.0	41.0
17	39.101906121177436	41.0	39.0	41.0	35.0	41.0
18	38.547729100406436	39.0	38.0	41.0	35.0	41.0
19	38.0487741706453	39.0	37.0	41.0	35.0	41.0
20	37.44932224387629	38.0	35.0	41.0	35.0	41.0
21	37.39697545443349	38.0	35.0	41.0	35.0	41.0
22	37.21478321584595	38.0	35.0	41.0	34.0	41.0
23	37.12148126480667	38.0	35.0	41.0	34.0	41.0
24	36.93321783828036	38.0	35.0	40.0	34.0	41.0
25	36.799068945656344	38.0	35.0	40.0	33.0	41.0
26	36.911342000594075	38.0	35.0	40.0	34.0	41.0
27	36.92231478529874	38.0	35.0	40.0	34.0	41.0
28	36.8537657847264	38.0	35.0	40.0	33.0	41.0
29	36.711384926356054	38.0	35.0	41.0	33.0	41.0
30	36.4165913503684	38.0	35.0	41.0	33.0	41.0
31	35.899727140673335	38.0	35.0	41.0	31.0	41.0
32	35.137889048678176	38.0	35.0	41.0	23.0	41.0
33	34.13520030211043	38.0	34.0	41.0	16.0	41.0
34	33.259116290779474	38.0	33.0	41.0	11.0	41.0
35	32.73159490034703	38.0	33.0	41.0	10.0	41.0
36	32.4898341109477	38.0	31.0	41.0	8.0	41.0
37	32.242563609821126	38.0	31.0	41.0	8.0	41.0
38	32.09656485231364	38.0	30.0	41.0	7.0	41.0
39	32.0089125292511	38.0	30.0	41.0	7.0	41.0
40	31.862485419730636	38.0	29.0	41.0	7.0	41.0
41	31.712979699918133	37.0	29.0	41.0	7.0	41.0
42	31.573405316998603	37.0	27.0	41.0	7.0	41.0
43	31.460585275558035	37.0	26.0	40.0	7.0	41.0
44	31.356888316947888	37.0	25.0	40.0	7.0	41.0
45	31.325998069246317	37.0	24.0	40.0	7.0	41.0
46	31.292271913873172	37.0	24.0	40.0	7.0	41.0
47	31.297779814386832	36.0	25.0	40.0	7.0	41.0
48	31.25574969752733	36.0	25.0	40.0	7.0	41.0
49	31.146877648899867	36.0	25.0	40.0	7.0	41.0
50	31.064692926848707	36.0	24.0	40.0	7.0	41.0
51	30.56628045555644	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	14.0
10	13.0
11	24.0
12	21.0
13	24.0
14	58.0
15	100.0
16	198.0
17	428.0
18	781.0
19	1475.0
20	2446.0
21	3828.0
22	6301.0
23	10646.0
24	20860.0
25	46346.0
26	88472.0
27	117219.0
28	111557.0
29	88066.0
30	66810.0
31	55072.0
32	50745.0
33	57481.0
34	95003.0
35	128271.0
36	141515.0
37	183336.0
38	359842.0
39	571140.0
40	362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.826670482289956	15.716579486919416	18.26495700178948	38.191793029001154
2	51.458162777387365	18.065949909077077	18.266949336733585	12.208937976801977
3	22.68735193328938	17.831714712125713	47.02906635562092	12.451866998963986
4	20.56574161951474	19.081497366495448	46.760961464619754	13.591799549370059
5	18.553302204609174	22.149512059060054	45.35410131204312	13.94308442428765
6	19.811733403849914	24.588084750306095	44.721534967289486	10.878646878554507
7	59.910643777756846	4.136857109737808	32.76018988763231	3.1923092248730343
8	61.35581109766788	3.529511914162966	32.34433524838983	2.7703417397793215
9	56.76705619833513	6.020202276333234	33.45723543603156	3.7555060893000745
10	28.42908012084417	24.21117120315296	38.3855928826551	8.974155793347775
11	20.64484637286367	18.94882597135385	47.093862521644006	13.31246513413848
12	20.32435212890045	17.019204297647597	48.40554340029994	14.250900173152017
13	17.773031391953864	17.432342116511748	49.52482811583073	15.269798375703655
14	14.278928703388418	20.14300436864717	49.0333553093915	16.544711618572908
15	14.174014156445386	19.863352991038116	51.129246390251325	14.833386462265178
16	16.655467329329344	19.148014185424802	50.26584087401923	13.930677611226628
17	16.834098269204297	18.959240449470762	48.078936310485474	16.12772497083946
18	16.450166269407156	19.87978975432699	48.33938882408769	15.330655152178165
19	17.926441182650024	20.158671366162185	47.00307544066826	14.91181201051953
20	18.932434488404613	19.886989328329555	47.72493461518956	13.455641568076274
21	18.212069565091394	19.563053778553783	47.87608038890378	14.348796267451041
22	17.51199023393635	18.514225271500916	47.696317440537854	16.27746705402488
23	16.700113744213173	19.407334690536047	48.13069173869259	15.761859826558187
24	16.819608560519892	18.41651029856045	48.774985691412674	15.988895449506987
25	16.168250874816163	19.68105434365242	47.582346825667074	16.568347955864347
26	15.646530801498235	21.164392989878937	47.40964761028479	15.77942859833803
27	15.948414825869925	21.449432727905005	47.67915619181476	14.922996254410306
28	15.246207318751855	20.855581073542517	48.311133892153094	15.587077715552528
29	15.913322562649878	19.37817415180868	47.99629969064472	16.712203594896724
30	16.732941990451284	19.8787483065153	47.45424874482899	15.934060958204435
31	17.080423316839216	21.123097320128377	45.169266965637654	16.62721239739475
32	17.283867882836216	22.03314158618841	43.72287707655638	16.960113454418998
33	18.339669562193453	23.424017778872557	41.059804461381304	17.17650819755269
34	17.349841337689906	24.2156539567772	40.59418672887581	17.84031797665708
35	18.094023719653116	25.15191553948808	37.65005904556289	19.104001695295917
36	18.583594751827516	27.059983771526277	36.66489469604214	17.691526780604075
37	19.278829086641213	27.295396257308248	36.451986539060634	16.97378811698991
38	18.837255214483914	27.02688384325033	35.26378514659963	18.87207579566613
39	19.400497359250593	26.011562787530156	35.882812669801275	18.70512718341798
40	19.240431358627536	26.172670235964908	36.5094020097226	18.077496395684964
41	17.617991509030713	27.92782676104297	35.47854979750632	18.975631932419997
42	18.65300951249375	27.846956074448126	34.44489020423244	19.05514420882568
43	19.59257655999826	27.162770142506286	34.55655152178166	18.6881017757138
44	18.735555571655233	27.00954147316868	34.60386947670417	19.651033478471916
45	18.54510646313456	26.24502821870766	34.90887784451094	20.300987473646842
46	18.235615341703554	26.887873200559305	35.46781835701193	19.40869310072521
47	17.798026139434466	26.29959102797238	37.05598098950221	18.846401843090945
48	17.949624716545074	25.086394888030778	37.26952307123865	19.694457324185496
49	18.230408102645097	24.53587651870259	37.65359091205471	19.5801244665976
50	16.90853914757044	24.43055444870281	38.50223503756457	20.158671366162185
51	16.99072296401481	24.158510168153068	37.19490107151396	21.655865796318167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	1018.5
2	1657.0
3	95254.5
4	188852.0
5	131645.5
6	74439.0
7	70637.0
8	66835.0
9	64064.0
10	61293.0
11	57818.5
12	54344.0
13	50316.0
14	46288.0
15	42359.0
16	38430.0
17	34683.0
18	30936.0
19	28516.0
20	26096.0
21	24136.5
22	22177.0
23	21546.0
24	20915.0
25	20366.5
26	21998.0
27	24178.0
28	26947.5
29	29717.0
30	32897.5
31	36078.0
32	40763.5
33	45449.0
34	50346.5
35	55244.0
36	58873.0
37	62502.0
38	66150.5
39	69799.0
40	76483.5
41	83168.0
42	89159.0
43	95150.0
44	98116.0
45	101082.0
46	105401.5
47	109721.0
48	115659.0
49	121597.0
50	125907.5
51	130218.0
52	125621.5
53	121025.0
54	106368.0
55	91711.0
56	85965.0
57	80219.0
58	76508.5
59	72798.0
60	69804.0
61	66810.0
62	60799.5
63	54789.0
64	47227.0
65	39665.0
66	31874.5
67	24084.0
68	19751.0
69	15418.0
70	12596.0
71	9774.0
72	8016.5
73	6259.0
74	5228.5
75	3353.0
76	2508.0
77	1931.5
78	1355.0
79	1060.0
80	765.0
81	625.0
82	485.0
83	319.0
84	153.0
85	98.5
86	44.0
87	32.5
88	21.0
89	15.0
90	9.0
91	7.5
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2208464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.849331139357105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94798826407467	23.565446444804433
2	8.857261452590839	5.287666601724942
3	3.159557353895574	2.8293202113065967
4	1.524449497314921	1.820151914023182
5	0.8664848214080624	1.2931996180717977
6	0.618350492319443	1.107440916325653
7	0.4111570276989978	0.8590933589042846
8	0.3314340656941161	0.7914468142213688
9	0.26836644879455307	0.7209503097085743
>10	3.824454370430163	29.46121865440067
>50	1.099061477741268	21.221004225219534
>100	0.08534954016550053	5.042879732535691
>500	0.0037447309980529835	0.7531023587788576
>1k	0.002184426415530907	1.0842904152585395
>5k	0.0	0.0
>10k+	1.5603045825220762E-4	4.162788424715788
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89380	4.047156756913402	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	2495	0.11297444739873505	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	2492	0.11283860637981873	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	2465	0.1116160372095719	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3584101891631468E-4	0.0	0.0	0.07090901187431627	0.0
2	1.3584101891631468E-4	0.0	0.0	0.3957954487825022	0.0
3	1.3584101891631468E-4	0.0	0.0	0.5889613776815017	0.0
4	1.3584101891631468E-4	0.0	0.0	0.7942171572640532	0.0
5	1.3584101891631468E-4	0.0	0.0	1.3496258032732251	0.0
6	1.3584101891631468E-4	0.0	0.0	1.6726104659165828	0.0
7	1.3584101891631468E-4	0.0	0.0	2.0035644683363643	0.0
8	1.3584101891631468E-4	0.0	0.0	2.50613095798709	0.0
9	1.3584101891631468E-4	0.0	0.0	2.6934557230726877	0.0
10	1.3584101891631468E-4	0.0	0.0	3.0998014909910236	0.0
11	1.3584101891631468E-4	0.0	0.0	3.709274862528889	0.0
12	1.3584101891631468E-4	0.0	0.0	4.22528961305233	0.0
13	1.3584101891631468E-4	0.0	0.0	4.419225307725188	0.0
14	1.3584101891631468E-4	0.0	0.0	4.496745249186765	0.0
15	1.3584101891631468E-4	0.0	0.0	4.649656956146897	0.0
16	1.3584101891631468E-4	0.0	0.0	4.974045309319056	0.0
17	1.3584101891631468E-4	0.0	0.0	5.362460062740438	0.0
18	1.3584101891631468E-4	0.0	0.0	5.778495823341472	0.0
19	1.3584101891631468E-4	0.0	0.0	6.050902346608321	0.0
20	1.3584101891631468E-4	0.0	0.0	6.331187648972318	0.0
21	1.3584101891631468E-4	0.0	0.0	6.677265284831448	0.0
22	1.3584101891631468E-4	0.0	0.0	7.069166624405017	0.0
23	1.3584101891631468E-4	0.0	0.0	7.43725050532859	0.0
24	1.8112135855508625E-4	0.0	0.0	7.7162679581827005	0.0
25	2.264016981938578E-4	0.0	0.0	7.971830195103927	0.0
26	2.264016981938578E-4	0.0	0.0	8.216027066775823	0.0
27	2.7168203783262937E-4	0.0	0.0	8.4667443073557	0.0
28	2.7168203783262937E-4	0.0	0.0	8.73036644473263	0.0
29	2.7168203783262937E-4	0.0	0.0	9.000961754413927	0.0
30	3.1696237747140093E-4	0.0	0.0	9.318331654942078	0.0
31	3.1696237747140093E-4	0.0	0.0	9.617046055539053	0.0
32	3.1696237747140093E-4	0.0	0.0	9.905617660056945	0.0
33	3.1696237747140093E-4	0.0	0.0	10.173586710039194	0.0
34	3.1696237747140093E-4	0.0	0.0	10.4528305645915	0.0
35	3.1696237747140093E-4	0.0	0.0	10.734972360880684	0.0
36	3.622427171101725E-4	0.0	0.0	11.03626774083707	0.0
37	3.622427171101725E-4	0.0	0.0	11.329276818639562	0.0
38	3.622427171101725E-4	0.0	0.0	11.613003426816103	0.0
39	4.528033963877156E-4	0.0	0.0	11.90687283107173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATTA	30	2.1664146E-6	45.000004	35
CGACATA	20	7.0347014E-4	45.0	32
TTACGCG	20	7.0347014E-4	45.0	1
CGTTTTT	62780	0.0	44.49108	1
CGCATCG	50	1.0822987E-9	40.500004	21
GTATACG	45	1.9297659E-8	40.0	1
GTTTTTT	70380	0.0	39.83376	2
GTCTCGA	40	3.4601544E-7	39.375	27
TATAGCG	75	0.0	39.0	1
GTTAGCG	100	0.0	38.250004	1
TCGACGT	60	1.5643309E-10	37.500004	26
CGAACTA	25	0.0021075278	36.000004	12
CGTAACG	25	0.0021075278	36.000004	1
TATCGCG	50	4.8825314E-8	36.000004	1
TGCGTCG	25	0.0021075278	36.000004	2
CCGATAC	70	2.1827873E-11	35.357143	11
GCGTTAG	210	0.0	35.357143	1
AATAGCG	70	2.1827873E-11	35.357143	1
TCGATCA	115	0.0	35.21739	17
CAATACG	45	8.7047556E-7	35.0	1
>>END_MODULE