##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934875.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1583659
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58812787348791	34.0	31.0	34.0	31.0	34.0
2	32.74806003060002	34.0	31.0	34.0	31.0	34.0
3	32.803452637215464	34.0	31.0	34.0	31.0	34.0
4	36.19412322981147	37.0	37.0	37.0	35.0	37.0
5	36.081539018185104	37.0	35.0	37.0	35.0	37.0
6	36.07238995263501	37.0	35.0	37.0	35.0	37.0
7	36.221643043104606	37.0	37.0	37.0	35.0	37.0
8	36.2284342778338	37.0	37.0	37.0	35.0	37.0
9	37.99355290501301	39.0	39.0	39.0	35.0	39.0
10	37.468756847275834	39.0	37.0	39.0	34.0	39.0
11	37.367016510498786	39.0	37.0	39.0	34.0	39.0
12	37.40693924639079	39.0	37.0	39.0	35.0	39.0
13	37.45769322815076	39.0	37.0	39.0	35.0	39.0
14	38.77242765014438	40.0	38.0	41.0	35.0	41.0
15	38.82712503133566	40.0	38.0	41.0	35.0	41.0
16	38.91622375776603	40.0	38.0	41.0	35.0	41.0
17	38.91654263954551	40.0	38.0	41.0	35.0	41.0
18	38.39887816758532	39.0	38.0	41.0	35.0	41.0
19	37.897165993436715	38.0	37.0	41.0	35.0	41.0
20	37.31492827685758	38.0	35.0	41.0	34.0	41.0
21	37.272916707447756	38.0	35.0	41.0	34.0	41.0
22	37.09529261034099	38.0	35.0	40.0	34.0	41.0
23	36.98642068778695	38.0	35.0	40.0	33.0	41.0
24	36.75080304535257	38.0	35.0	40.0	33.0	41.0
25	36.602559010494055	37.0	35.0	40.0	33.0	41.0
26	36.731531851238174	38.0	35.0	40.0	33.0	41.0
27	36.75129620707488	38.0	35.0	40.0	33.0	41.0
28	36.689710979446964	38.0	35.0	40.0	33.0	41.0
29	36.549007077912606	38.0	35.0	40.0	33.0	41.0
30	36.22445488580559	38.0	35.0	40.0	32.0	41.0
31	35.73467836194534	38.0	35.0	40.0	30.0	41.0
32	34.99535253485757	38.0	35.0	40.0	23.0	41.0
33	33.98575640336714	38.0	34.0	40.0	16.0	41.0
34	33.17096041508936	38.0	33.0	40.0	12.0	41.0
35	32.6596009620758	38.0	32.0	41.0	10.0	41.0
36	32.41059407359791	38.0	31.0	41.0	8.0	41.0
37	32.181397636738716	38.0	31.0	40.0	8.0	41.0
38	32.02017290338387	38.0	30.0	40.0	7.0	41.0
39	31.93962147154154	37.0	30.0	40.0	7.0	41.0
40	31.8044686387663	37.0	29.0	40.0	7.0	41.0
41	31.686789896057167	37.0	29.0	40.0	7.0	41.0
42	31.570960667668988	37.0	28.0	40.0	7.0	41.0
43	31.463467829880045	37.0	27.0	40.0	7.0	41.0
44	31.363377469518376	37.0	26.0	40.0	7.0	41.0
45	31.3277460614943	37.0	25.0	40.0	7.0	41.0
46	31.27359867244148	36.0	25.0	40.0	7.0	41.0
47	31.26286403828097	36.0	25.0	40.0	7.0	41.0
48	31.22124207294626	36.0	25.0	40.0	7.0	41.0
49	31.11250275469656	36.0	25.0	40.0	7.0	41.0
50	31.032512681075914	36.0	25.0	40.0	7.0	41.0
51	30.50091528542445	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	2.0
9	18.0
10	16.0
11	23.0
12	19.0
13	28.0
14	40.0
15	91.0
16	179.0
17	313.0
18	595.0
19	1134.0
20	1900.0
21	3105.0
22	5066.0
23	8651.0
24	16401.0
25	35390.0
26	64464.0
27	82315.0
28	77130.0
29	60982.0
30	46555.0
31	40266.0
32	38655.0
33	45172.0
34	74929.0
35	98364.0
36	107330.0
37	142756.0
38	270980.0
39	360596.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.090732285169977	15.394223125054069	18.12757670685419	37.38746788292177
2	50.95408796969549	17.987837028047075	18.41040274453023	12.6476722577272
3	23.165908822543237	17.99989770525094	46.591280067236696	12.242913404969125
4	20.776505548227238	19.027896788386894	46.3928156250809	13.802782038304965
5	19.290200731344314	21.749757997144588	44.888325075031936	14.071716196479167
6	19.673869185222323	24.431585334974258	44.48804951065854	11.406495969144872
7	58.751726223890365	4.590508436475277	32.95507429314012	3.702691046494226
8	59.644784641138024	4.1947161604865695	32.46405949765701	3.6964397007184
9	55.12941864378632	5.528589172290247	34.31426841258124	5.027723771342189
10	30.217363712768975	17.99352006966146	41.64621297893044	10.142903238639127
11	22.12685938071264	18.408445252418606	46.08504734920839	13.379648017660367
12	19.918050539920525	17.350768063074185	48.12557501330779	14.6056063836975
13	17.576637394792694	18.420316494901996	48.802804139022356	15.20024197128296
14	14.734611428344108	21.08004311534238	47.8469165394823	16.338428916831212
15	14.286787749130337	20.08462680412892	50.84086915175552	14.78771629498522
16	16.078145610892243	18.917203766719982	49.37192918424989	15.632721438137882
17	16.110223223560123	18.92396027175042	47.44790387324544	17.517912631444016
18	15.966252835995629	19.458355618223365	48.44862435663233	16.126767189148676
19	17.460766490766005	20.028238402332825	46.934977795093516	15.576017311807655
20	18.567633562528297	19.962820278860537	46.93213627428632	14.537409884324845
21	17.69320289279447	19.84366583967887	47.29913447276213	15.163996794764529
22	17.01407941987511	18.761677861206234	46.79864794125503	17.425594777663626
23	16.546175660290505	19.528635899521298	47.079137617378485	16.84605082280971
24	16.54169237190582	18.745260185431334	47.77139523091777	16.94165221174508
25	15.68014326316461	20.58454503147458	46.26684153596197	17.46847016939884
26	15.06347010309669	21.109152917389412	46.57138942158634	17.25598755792756
27	16.330598948384722	21.358449009540564	46.25793810409943	16.053013937975283
28	15.405841787910148	20.705530672954215	47.29667182139589	16.59195571773974
29	16.37871536738654	19.39476869704905	46.583513243697034	17.64300269186738
30	16.2008361648562	21.48650688058477	45.11722536227812	17.19543159228091
31	17.27543618923013	21.46270125071117	43.579962605586175	17.68189995447252
32	17.992004591897626	22.174849509900806	42.528599906924406	17.304545991277163
33	17.814314824087763	23.75044122503645	40.08710208447652	18.34814186639927
34	17.64117148956941	23.590116306603885	38.97884582476404	19.789866379062666
35	17.715051030556452	24.28534173076401	37.13993984816176	20.85966739051778
36	18.39821577751271	26.793710009541194	35.007725779350224	19.800348433595868
37	19.365658895002017	26.191939047484336	36.079484282916965	18.362917774596678
38	18.97693884857788	26.558242651985054	34.76404958390663	19.700768915530425
39	19.566522843617218	25.941190622476178	34.530539718462116	19.961746815444485
40	18.475126274027424	25.77562467677701	36.14326063881176	19.6059884103838
41	16.989389761305937	26.519029664845778	34.497578077098666	21.994002496749616
42	18.128271300829283	26.323659323124488	34.404502484436364	21.143566891609872
43	19.486897116108963	26.159987724630113	33.9859780419901	20.367137117270826
44	19.171930320858216	25.925467540676372	33.95307954553348	20.949522592931938
45	18.69322878220627	25.15819377782717	33.90344764876782	22.245129791198735
46	18.86915049262499	25.73445419752611	34.99086608922754	20.405529220621357
47	17.354872482017907	25.03127251510584	37.019648800657215	20.594206202219038
48	17.7591893204282	23.933940324274356	36.32252902929229	21.984341326005154
49	18.082743823007352	23.58999001679023	37.26515619839877	21.062109961803646
50	17.316101509226417	23.03652490845567	37.54078371669659	22.106589865621324
51	17.130771207690547	23.34940792178114	36.132273425023946	23.387547445504367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	403.0
1	829.0
2	1255.0
3	61927.0
4	122599.0
5	86073.5
6	49548.0
7	47220.5
8	44893.0
9	43010.0
10	41127.0
11	39340.0
12	37553.0
13	35622.0
14	33691.0
15	30769.0
16	27847.0
17	25268.5
18	22690.0
19	21089.5
20	19489.0
21	18038.0
22	16587.0
23	15210.5
24	13834.0
25	14276.0
26	14950.0
27	15182.0
28	16978.0
29	18774.0
30	20754.5
31	22735.0
32	24839.5
33	26944.0
34	29545.0
35	32146.0
36	34729.0
37	37312.0
38	40155.0
39	42998.0
40	49164.5
41	55331.0
42	59286.0
43	63241.0
44	69274.5
45	75308.0
46	79231.5
47	83155.0
48	91620.5
49	100086.0
50	108704.0
51	117322.0
52	113164.5
53	109007.0
54	92597.5
55	76188.0
56	66978.0
57	57768.0
58	54493.5
59	51219.0
60	49166.0
61	47113.0
62	41938.5
63	36764.0
64	30768.5
65	24773.0
66	19307.0
67	13841.0
68	12264.0
69	10687.0
70	9002.5
71	7318.0
72	6270.5
73	5223.0
74	4041.0
75	2347.0
76	1835.0
77	1470.5
78	1106.0
79	842.0
80	578.0
81	538.5
82	499.0
83	284.0
84	69.0
85	47.5
86	26.0
87	14.5
88	3.0
89	5.0
90	7.0
91	5.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1583659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.80913659455844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57935761847	26.567002352355086
2	9.183197851406508	6.209519810661162
3	3.362312514188348	3.4103064919736115
4	1.6274755627147097	2.200941744165099
5	0.948211697927241	1.6029109407890134
6	0.6369516421730314	1.2920871044613793
7	0.47381335906899424	1.1213454403963132
8	0.36645956336979435	0.9911745147481296
9	0.29579734214851316	0.9000587470505775
>10	3.8490889127137122	32.360663531967425
>50	0.595186293637265	12.48213955862273
>100	0.07566731912226046	4.630683897706365
>500	0.0036213570039733646	0.8101327561694812
>1k	0.002668368318717216	1.5254106430197232
>5k	0.0	0.0
>10k+	1.905977370512297E-4	3.895622465913921
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60454	3.8173621972912097	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	4138	0.26129362444819243	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3375	0.21311406053954796	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1993	0.12584779930527973	TruSeq Adapter, Index 8 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1593	0.10058983657466664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5257962730613093E-4	0.0	0.0	0.13494066588830045	0.0
2	2.5257962730613093E-4	0.0	0.0	0.8100860096775884	0.0
3	2.5257962730613093E-4	0.0	0.0	1.157572431943998	0.0
4	3.1572453413266364E-4	0.0	0.0	1.5325268886799495	0.0
5	3.1572453413266364E-4	0.0	0.0	2.5346365600170238	0.0
6	3.1572453413266364E-4	0.0	0.0	3.0579184028885007	0.0
7	3.1572453413266364E-4	0.0	0.0	3.648702151157541	0.0
8	3.1572453413266364E-4	0.0	0.0	4.53563551244302	0.0
9	3.1572453413266364E-4	0.0	0.0	4.87017722880999	0.0
10	3.1572453413266364E-4	0.0	0.0	5.64654385824221	0.0
11	3.1572453413266364E-4	0.0	0.0	6.684772416284061	0.0
12	3.788694409591964E-4	0.0	0.0	7.561728882290947	0.0
13	3.788694409591964E-4	0.0	0.0	7.870507476672693	0.0
14	3.788694409591964E-4	0.0	0.0	8.004374679144941	0.0
15	3.788694409591964E-4	0.0	0.0	8.253102467134655	0.0
16	3.788694409591964E-4	0.0	0.0	8.786487495098376	0.0
17	3.788694409591964E-4	0.0	0.0	9.45405545006848	0.0
18	4.420143477857291E-4	0.0	0.0	10.179779864225821	0.0
19	4.420143477857291E-4	0.0	0.0	10.657155359834409	0.0
20	4.420143477857291E-4	0.0	0.0	11.066460645883994	0.0
21	4.420143477857291E-4	0.0	0.0	11.574398276396623	0.0
22	4.420143477857291E-4	0.0	0.0	12.128747413426755	0.0
23	5.051592546122619E-4	0.0	0.0	12.665415976545455	0.0
24	5.051592546122619E-4	0.0	0.0	13.111787322902215	0.0
25	5.051592546122619E-4	0.0	0.0	13.506253555847566	0.0
26	5.051592546122619E-4	0.0	0.0	13.87041023351618	0.0
27	5.051592546122619E-4	0.0	0.0	14.219096409012293	0.0
28	5.051592546122619E-4	0.0	0.0	14.578706653389398	0.0
29	5.051592546122619E-4	0.0	0.0	14.961617368385491	0.0
30	5.683041614387946E-4	0.0	0.0	15.360566889715527	0.0
31	5.683041614387946E-4	0.0	0.0	15.753201920362907	0.0
32	6.945939750918601E-4	0.0	0.0	16.129292985421735	0.0
33	6.945939750918601E-4	0.0	0.0	16.49123959135142	0.0
34	7.577388819183928E-4	0.0	0.0	16.851986444051402	0.0
35	7.577388819183928E-4	0.0	0.0	17.21355418054013	0.0
36	7.577388819183928E-4	0.0	0.0	17.58964524559896	0.0
37	7.577388819183928E-4	0.0	0.0	17.98587953593545	0.0
38	7.577388819183928E-4	0.0	0.0	18.36323349913081	0.0
39	7.577388819183928E-4	0.0	0.0	18.71823416530958	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	25	3.8914754E-5	45.000004	1
TAGACGC	20	7.034095E-4	45.0	1
AATGCGT	20	7.034095E-4	45.0	13
CGCGTAA	45	3.8562575E-10	45.0	31
GCGTAAC	45	3.8562575E-10	45.0	32
CGTTTTT	44010	0.0	44.493862	1
TGCGACG	50	1.0822987E-9	40.500004	1
GTTTTTT	48945	0.0	40.17775	2
GGTACGA	45	1.9290383E-8	40.0	9
GTCGATG	40	3.4593177E-7	39.375	1
GTATGCG	40	3.4593177E-7	39.375	1
TATAGCG	80	0.0	39.375	1
CAATGCG	40	3.4593177E-7	39.375	1
TACGTAG	40	3.4593177E-7	39.375	1
AATTGCG	110	0.0	38.863636	1
GTCGCAC	30	1.1401168E-4	37.499996	25
ATCGTAG	30	1.1401168E-4	37.499996	1
CGCACAT	30	1.1401168E-4	37.499996	30
TCTAGCG	55	2.750312E-9	36.81818	1
TACGAGG	165	0.0	36.81818	2
>>END_MODULE