##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934873.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	863603
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44499266445346	34.0	31.0	34.0	30.0	34.0
2	32.61569957492042	34.0	31.0	34.0	31.0	34.0
3	32.68077345724829	34.0	31.0	34.0	31.0	34.0
4	36.11441136726019	37.0	37.0	37.0	35.0	37.0
5	35.98164202764465	37.0	35.0	37.0	35.0	37.0
6	35.96967124940511	37.0	35.0	37.0	35.0	37.0
7	36.135195222804924	37.0	36.0	37.0	35.0	37.0
8	36.13709887529339	37.0	37.0	37.0	35.0	37.0
9	37.89186813848493	39.0	38.0	39.0	35.0	39.0
10	37.54266254285823	39.0	37.0	39.0	35.0	39.0
11	37.43810176666825	39.0	37.0	39.0	34.0	39.0
12	37.325472468252194	39.0	37.0	39.0	34.0	39.0
13	37.23495402401335	39.0	37.0	39.0	34.0	39.0
14	38.4409248230958	40.0	38.0	41.0	34.0	41.0
15	38.50476665782773	40.0	38.0	41.0	34.0	41.0
16	38.59630987849741	40.0	38.0	41.0	34.0	41.0
17	38.6103082087487	40.0	38.0	41.0	34.0	41.0
18	38.154120585500515	39.0	38.0	40.0	34.0	41.0
19	37.70809735491887	38.0	37.0	40.0	34.0	41.0
20	37.1803409668563	38.0	35.0	40.0	34.0	41.0
21	37.117775181420164	38.0	35.0	40.0	34.0	41.0
22	36.930119510932684	38.0	35.0	40.0	33.0	41.0
23	36.79379182332623	38.0	35.0	40.0	33.0	41.0
24	36.565148569423684	37.0	35.0	40.0	33.0	41.0
25	36.43034936191746	37.0	35.0	40.0	33.0	41.0
26	36.567117066522464	37.0	35.0	40.0	33.0	41.0
27	36.653765677052995	37.0	35.0	40.0	33.0	41.0
28	36.60533833254401	37.0	35.0	40.0	33.0	41.0
29	36.46027283369789	37.0	35.0	40.0	33.0	41.0
30	36.213813523111895	37.0	35.0	40.0	32.0	41.0
31	35.71731455309905	37.0	35.0	40.0	31.0	41.0
32	35.05377239310192	37.0	35.0	40.0	25.0	41.0
33	34.245068625282684	37.0	34.0	40.0	18.0	41.0
34	33.500124478493014	37.0	34.0	40.0	15.0	41.0
35	33.03251262443507	37.0	33.0	40.0	10.0	41.0
36	32.860462504183054	37.0	33.0	40.0	10.0	41.0
37	32.645121658910405	37.0	33.0	40.0	10.0	41.0
38	32.51432313227259	37.0	32.0	40.0	10.0	41.0
39	32.428818565938286	37.0	32.0	40.0	9.0	41.0
40	32.27619172235391	37.0	31.0	40.0	8.0	41.0
41	32.14974241636493	37.0	31.0	40.0	8.0	41.0
42	32.051294402636394	37.0	31.0	40.0	8.0	41.0
43	31.956308627922784	37.0	31.0	40.0	8.0	41.0
44	31.821322992161907	36.0	30.0	40.0	8.0	41.0
45	31.780497520272625	36.0	30.0	40.0	8.0	41.0
46	31.735194296453347	36.0	30.0	40.0	8.0	41.0
47	31.73162900082561	36.0	30.0	40.0	8.0	41.0
48	31.73333348772526	36.0	30.0	40.0	8.0	41.0
49	31.66253359471887	36.0	29.0	40.0	8.0	41.0
50	31.572628858399057	35.0	29.0	40.0	8.0	41.0
51	30.93712736060435	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	5.0
11	9.0
12	16.0
13	13.0
14	17.0
15	32.0
16	74.0
17	131.0
18	303.0
19	556.0
20	1019.0
21	1623.0
22	2637.0
23	4144.0
24	7836.0
25	16375.0
26	29916.0
27	39804.0
28	38157.0
29	30862.0
30	24949.0
31	22829.0
32	23119.0
33	27990.0
34	50413.0
35	67353.0
36	68285.0
37	86268.0
38	153446.0
39	165329.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.81381491263926	16.29047143189637	18.74900851432892	35.146705141135456
2	48.91703711080207	18.953963800496293	18.388426163410735	13.740572925290904
3	23.44642156175928	18.49090380649442	44.531225574714306	13.531449057031994
4	22.50849059116284	19.630547832742593	43.376644129304786	14.484317446789788
5	18.7990314994274	22.810481204905493	43.22356453138769	15.16692276427942
6	20.221907520006297	24.796926365471172	42.987344879533765	11.993821234988761
7	58.25755584452579	5.477169486442265	31.492942937900864	4.772331731131087
8	57.56568701127718	4.91522146171331	31.677518489398487	5.841573037611032
9	52.112139490020304	7.114495896841488	34.21769030445703	6.555674308681188
10	30.279074991633887	19.740899464221407	38.64831409802884	11.331711446115866
11	23.791371729834196	18.125805491643728	42.42597582453975	15.656846953982328
12	21.98487036288665	15.66587888184733	46.80727139669501	15.541979358571009
13	18.54208473106277	19.78698545512232	47.00423690052026	14.666692913294652
14	14.66970355591632	22.008723915966016	46.22448046150836	17.09709206660931
15	13.351273675519886	19.541502287509424	51.41332301995245	15.693901017018236
16	14.637281250759898	19.908105923670945	48.32648798116727	17.128124844401885
17	15.103930857118375	19.29972452620012	45.225410286902665	20.370934329778844
18	15.732691989258955	19.47677347114357	47.68973706668458	17.1007974729129
19	17.28340452731174	20.136798968970695	45.567465606302896	17.012330897414667
20	18.135184801349695	21.03941278573604	45.2703383383337	15.555064074580566
21	17.500981353700716	20.852868737139634	46.04465246183721	15.601497447322437
22	16.41136031255102	20.214959883187067	43.66358152993911	19.710098274322807
23	15.568727760325057	20.254445619109706	45.07812038633492	19.098706234230313
24	17.06061697330834	18.752250744844563	45.909405131756145	18.277727150090957
25	15.178038983190193	20.47051712418785	44.75007613452014	19.601367758101812
26	14.094439227283834	22.596609784820107	44.65755677087736	18.651394217018698
27	16.47713127443976	21.171996855036397	43.94391867559515	18.40695319492869
28	14.374892166886868	21.37614158357486	45.46996710293966	18.77899914659861
29	15.204092621262316	21.814653260815444	44.59595439108016	18.38529972684208
30	17.51487662733918	20.997147995085705	42.89019375801149	18.59778161956362
31	18.619087705809267	21.959858870337413	41.176559136547695	18.244494287305628
32	17.606353845459083	23.97293663871015	39.352225501764124	19.06848401406665
33	18.918878234559166	22.17454084805171	38.886618040928525	20.019962876460596
34	17.402093322973634	23.443410919137612	39.4610718119321	19.69342394595665
35	16.66043309252052	24.88770881990915	36.11439515610761	22.33746293146272
36	19.60183093388976	24.283148622688895	35.922408792002805	20.192611651418535
37	19.00688163426945	25.751068488645824	36.52824272263992	18.713807154444808
38	18.6930800379341	26.835015626393144	33.607340409887414	20.864563925785344
39	20.23221318128816	24.691553873712806	36.28426487633785	18.79196806866118
40	20.124409016643064	24.17499707620284	36.07560418386689	19.624989723287207
41	16.924211703757397	25.203942089131232	33.93862689221784	23.933219314893535
42	18.042897025600883	24.78882078918207	34.62586396758696	22.542418217630093
43	19.988351128933086	23.789403232735413	34.491658783028775	21.730586855302725
44	18.901045966723135	24.850886344767215	33.76482017779003	22.483247510719625
45	18.26973736774884	23.96911543845957	34.15851959754656	23.602627596245036
46	20.006414984663092	23.156126136662333	35.74987581099185	21.08758306768272
47	16.832966073531473	23.06719638537615	38.859290669439545	21.240546871652832
48	16.908579520914124	22.04809385794167	37.58092549470069	23.462401126443517
49	18.31952876495334	20.303194870791323	40.115307612409865	21.261968751845465
50	17.48627552243334	20.688093950576828	38.540509933383746	23.28512059360609
51	16.59107251827518	20.827162480908473	35.96397881897121	26.617786181845133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	608.0
1	759.0
2	910.0
3	33396.0
4	65882.0
5	44587.0
6	23292.0
7	22230.0
8	21168.0
9	20933.5
10	20699.0
11	19702.5
12	18706.0
13	17471.0
14	16236.0
15	14547.5
16	12859.0
17	11655.5
18	10452.0
19	9542.5
20	8633.0
21	8295.0
22	7957.0
23	7275.0
24	6593.0
25	7135.5
26	7496.5
27	7315.0
28	8081.0
29	8847.0
30	9586.5
31	10326.0
32	11715.0
33	13104.0
34	13616.0
35	14128.0
36	15332.5
37	16537.0
38	17950.0
39	19363.0
40	21986.0
41	24609.0
42	30356.5
43	36104.0
44	40925.5
45	45747.0
46	55247.5
47	64748.0
48	72091.5
49	79435.0
50	79224.5
51	79014.0
52	65863.5
53	52713.0
54	44616.0
55	36519.0
56	33233.0
57	29947.0
58	27058.5
59	24170.0
60	22965.0
61	21760.0
62	19766.5
63	17773.0
64	15447.5
65	13122.0
66	10492.5
67	7863.0
68	6847.5
69	5832.0
70	5244.0
71	4656.0
72	3906.5
73	3157.0
74	2640.0
75	1750.0
76	1377.0
77	1181.0
78	985.0
79	670.0
80	355.0
81	269.5
82	184.0
83	118.5
84	53.0
85	33.0
86	13.0
87	12.5
88	12.0
89	22.0
90	32.0
91	19.5
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	863603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.438462197973664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72400377589187	25.733797793172936
2	10.824435623839216	7.455538340919726
3	4.383116833352109	4.5284341012409595
4	2.328536582435888	3.207648762832685
5	1.3765180351822326	2.3702582159726147
6	0.8998599264945975	1.8593875237233333
7	0.6339384740422848	1.5282306321902432
8	0.46119249831203457	1.2706208335286429
9	0.352838411576969	1.0936091069177871
>10	3.430981605875052	28.324939767100226
>50	0.4997422691205064	10.981389299989598
>100	0.07796665506130201	4.7634236622363275
>500	0.00412158528958417	0.9330258957492921
>1k	0.0024042580855907656	1.918215130751302
>5k	0.0	0.0
>10k+	3.4346544079868086E-4	4.031480933674332
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34083	3.946605095165255	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	4343	0.5028931117654756	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	3983	0.46120729085007806	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2529	0.2928428919306672	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1545	0.17890164809524747	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	1392	0.16118517420620354	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC	1248	0.14451084584004456	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1177	0.13628947560395227	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3158789397443038E-4	0.0	0.0	0.3118330992365705	0.0
2	2.3158789397443038E-4	0.0	0.0	1.6747278552760934	0.0
3	2.3158789397443038E-4	0.0	0.0	2.386397453459518	0.0
4	2.3158789397443038E-4	0.0	0.0	3.116594083160897	0.0
5	2.3158789397443038E-4	0.0	0.0	4.92934832324575	0.0
6	2.3158789397443038E-4	0.0	0.0	5.866005560425334	0.0
7	2.3158789397443038E-4	0.0	0.0	6.880244742086353	0.0
8	2.3158789397443038E-4	0.0	0.0	8.409188018105542	0.0
9	2.3158789397443038E-4	0.0	0.0	9.004600493513802	0.0
10	2.3158789397443038E-4	0.0	0.0	10.225763458440973	0.0
11	2.3158789397443038E-4	0.0	0.0	12.07788764050148	0.0
12	2.3158789397443038E-4	0.0	0.0	13.530870087297057	0.0
13	2.3158789397443038E-4	0.0	0.0	14.077301723129725	0.0
14	2.3158789397443038E-4	0.0	0.0	14.300089277133127	0.0
15	2.3158789397443038E-4	0.0	0.0	14.757822749573588	0.0
16	2.3158789397443038E-4	0.0	0.0	15.636698807206551	0.0
17	2.3158789397443038E-4	0.0	0.0	16.755963098784974	0.0
18	2.3158789397443038E-4	0.0	0.0	18.124415964279883	0.0
19	2.3158789397443038E-4	0.0	0.0	18.815474239899583	0.0
20	2.3158789397443038E-4	0.0	0.0	19.512438006815632	0.0
21	2.3158789397443038E-4	0.0	0.0	20.359007553239163	0.0
22	2.3158789397443038E-4	0.0	0.0	21.14872227169197	0.0
23	2.3158789397443038E-4	0.0	0.0	21.909372709450984	0.0
24	2.3158789397443038E-4	0.0	0.0	22.51543822798207	0.0
25	2.3158789397443038E-4	0.0	0.0	23.074491404036344	0.0
26	2.3158789397443038E-4	0.0	0.0	23.601353862828173	0.0
27	2.3158789397443038E-4	0.0	0.0	24.080740803355244	0.0
28	2.3158789397443038E-4	0.0	0.0	24.587686703265273	0.0
29	2.3158789397443038E-4	0.0	0.0	25.127749672013646	0.0
30	2.3158789397443038E-4	0.0	0.0	25.703940352222027	0.0
31	2.3158789397443038E-4	0.0	0.0	26.267393698261817	0.0
32	2.3158789397443038E-4	0.0	0.0	26.791708690219927	0.0
33	2.3158789397443038E-4	0.0	0.0	27.283832964915593	0.0
34	2.3158789397443038E-4	0.0	0.0	27.77039913015587	0.0
35	2.3158789397443038E-4	0.0	0.0	28.29471412211398	0.0
36	2.3158789397443038E-4	0.0	0.0	28.80826027700228	0.0
37	2.3158789397443038E-4	0.0	0.0	29.33257526896039	0.0
38	2.3158789397443038E-4	0.0	0.0	29.827594392330735	0.0
39	2.3158789397443038E-4	0.0	0.0	30.322381927807104	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.03231E-4	45.000004	25
ATCCTTA	20	7.03231E-4	45.000004	35
CGAACCC	20	7.03231E-4	45.000004	40
CGTATAG	20	7.03231E-4	45.000004	31
CTCCGCG	20	7.03231E-4	45.000004	30
CGCATTG	20	7.03231E-4	45.000004	1
ATTACGG	35	1.2114651E-7	45.000004	2
ATTCGGT	20	7.03231E-4	45.000004	18
TAACGAT	20	7.03231E-4	45.000004	1
ACCTATC	20	7.03231E-4	45.000004	38
ACGATAT	20	7.03231E-4	45.000004	32
TACGATA	20	7.03231E-4	45.000004	31
TATTCGG	20	7.03231E-4	45.000004	17
CTTTGCG	35	1.2114651E-7	45.000004	1
TTACGTT	20	7.03231E-4	45.000004	20
CTCGAGG	20	7.03231E-4	45.000004	2
CTCGACG	20	7.03231E-4	45.000004	1
AGTTACG	20	7.03231E-4	45.000004	1
TTACGAT	20	7.03231E-4	45.000004	30
CCGCGAT	20	7.03231E-4	45.000004	10
>>END_MODULE