FastQCFastQC Report
Sat 14 Jan 2017
SRR2934871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535929
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT553543.603942630160639No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC45350.29526104396752717No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC42810.27872382121829853No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT23110.15046268414750943Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG18250.11882059652496958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG302.1659798E-645.0000041
TCCGATC253.8914208E-545.014
TATTGCG207.0340297E-445.01
ACGATAA207.0340297E-445.034
CCGTACT253.8914208E-545.041
ATCGGCG207.0340297E-445.03
AGTACGT253.8914208E-545.010
CGTTTTT426600.044.504221
CGAATAT2050.043.9024414
GCTACGA2100.042.85714310
ATCCGCC501.0822987E-940.518
GTTTTTT475900.040.059362
CGGTCTA451.9290383E-840.031
AGCTACG2250.039.09
ATTAACG356.24966E-638.571431
ACGGGTA2450.038.571435
TCTACGG356.24966E-638.571432
GTATGCG356.24966E-638.571431
CGAATTA356.24966E-638.5714337
GTCCAAT356.24966E-638.5714344
Produced by FastQC (version 0.11.5)