##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934871.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1535929
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61131080928871	34.0	31.0	34.0	31.0	34.0
2	32.76640652009305	34.0	31.0	34.0	31.0	34.0
3	32.81593940865756	34.0	31.0	34.0	31.0	34.0
4	36.21300203329711	37.0	37.0	37.0	35.0	37.0
5	36.09882683379245	37.0	35.0	37.0	35.0	37.0
6	36.0874669336929	37.0	36.0	37.0	35.0	37.0
7	36.23281675129515	37.0	37.0	37.0	35.0	37.0
8	36.22388665101056	37.0	37.0	37.0	35.0	37.0
9	37.98941747958402	39.0	39.0	39.0	35.0	39.0
10	37.62044534610649	39.0	38.0	39.0	35.0	39.0
11	37.548852844109334	39.0	37.0	39.0	35.0	39.0
12	37.52076691044964	39.0	37.0	39.0	35.0	39.0
13	37.484034743793494	39.0	37.0	39.0	35.0	39.0
14	38.80110473856539	40.0	38.0	41.0	35.0	41.0
15	38.83709142805429	40.0	38.0	41.0	35.0	41.0
16	38.928531201637576	40.0	38.0	41.0	35.0	41.0
17	38.958268904356906	40.0	38.0	41.0	35.0	41.0
18	38.42317255550224	39.0	38.0	41.0	35.0	41.0
19	37.91907568644123	38.0	37.0	41.0	35.0	41.0
20	37.34307835843975	38.0	35.0	41.0	34.0	41.0
21	37.287453391400255	38.0	35.0	41.0	34.0	41.0
22	37.082210831360044	38.0	35.0	41.0	34.0	41.0
23	36.98062866187174	38.0	35.0	40.0	34.0	41.0
24	36.769144276851335	37.0	35.0	40.0	33.0	41.0
25	36.60523045010544	37.0	35.0	40.0	33.0	41.0
26	36.72691055380815	38.0	35.0	40.0	33.0	41.0
27	36.76093686622233	38.0	35.0	40.0	33.0	41.0
28	36.69673467979314	38.0	35.0	40.0	33.0	41.0
29	36.54191437234403	38.0	35.0	40.0	33.0	41.0
30	36.22025367057982	38.0	35.0	40.0	32.0	41.0
31	35.68830785798041	38.0	35.0	40.0	30.0	41.0
32	34.90049214514473	38.0	35.0	40.0	22.0	41.0
33	33.86945099675831	38.0	34.0	40.0	15.0	41.0
34	32.99871087791168	38.0	33.0	40.0	10.0	41.0
35	32.50499860345107	38.0	32.0	40.0	9.0	41.0
36	32.275654668933264	38.0	31.0	40.0	8.0	41.0
37	32.002590614540125	37.0	30.0	40.0	7.0	41.0
38	31.850959256580218	37.0	30.0	40.0	7.0	41.0
39	31.77270303510123	37.0	29.0	40.0	7.0	41.0
40	31.64834963074465	37.0	29.0	40.0	7.0	41.0
41	31.521589214084766	37.0	28.0	40.0	7.0	41.0
42	31.389982870301946	37.0	27.0	40.0	7.0	41.0
43	31.235144983915273	37.0	25.0	40.0	7.0	41.0
44	31.114285230632404	36.0	24.0	40.0	7.0	41.0
45	31.09174903267013	36.0	24.0	40.0	7.0	41.0
46	31.058171959771578	36.0	24.0	40.0	7.0	41.0
47	31.03483819890112	36.0	24.0	40.0	7.0	41.0
48	30.973567137543466	35.0	24.0	40.0	7.0	41.0
49	30.821589409406293	35.0	24.0	40.0	7.0	41.0
50	30.729128104228778	35.0	24.0	40.0	7.0	41.0
51	30.207902188187084	35.0	24.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	16.0
11	10.0
12	9.0
13	27.0
14	47.0
15	54.0
16	121.0
17	257.0
18	526.0
19	1063.0
20	1846.0
21	2942.0
22	4653.0
23	8085.0
24	16248.0
25	35116.0
26	65243.0
27	83480.0
28	77827.0
29	59193.0
30	44094.0
31	37168.0
32	36064.0
33	43366.0
34	72662.0
35	98524.0
36	107706.0
37	141715.0
38	256864.0
39	340769.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.914878226793032	15.145036001013068	17.74255190181317	38.19753387038073
2	51.25302015913496	17.408226552138803	18.27167792261231	13.067075366113928
3	22.55501393619106	17.991847279398982	46.29608530081794	13.157053483592016
4	21.098501297911557	18.84077974958478	46.13826550576231	13.922453446741354
5	18.505673113796277	21.53328702042868	45.24245586872831	14.71858399704674
6	19.84134683308929	24.5002861460393	44.18967282992899	11.46869419094242
7	57.83854592237011	4.86500352555359	33.40167416592824	3.8947763861480578
8	57.68144230625244	4.6485872719377	33.35427614167061	4.315694280139251
9	52.84756001091196	6.699853964603833	35.085345741893015	5.367240282591188
10	28.646376232234694	20.367868566841306	40.30798298619272	10.677772214731279
11	22.702677011762916	19.125298109482923	44.059263156044324	14.112761722709838
12	21.903681745705693	16.30778506037714	47.32126289691776	14.467270296999404
13	18.46179087705226	18.326302843425704	47.92220213304131	15.289704146480728
14	14.295908209298739	21.465119806970247	47.17548792945507	17.063484054275946
15	13.434214732582038	19.731771455581608	51.70499417616309	15.129019635673263
16	14.35710895490612	18.8784116974157	51.119941091027	15.644538256651186
17	15.399474845516947	18.335417848090636	47.54959376377424	18.715513542618183
18	15.449021406588455	19.656637774272117	47.9709023008225	16.923438518316928
19	17.709477456314712	19.842388547908136	46.41855189920888	16.029582096568266
20	20.017852387708025	19.096650951964577	46.422653651308096	14.462843009019297
21	18.35429892918227	19.179141744182186	46.76713572046624	15.699423606169297
22	16.414691043661524	19.37016619908863	45.420523995575316	18.79461876167453
23	16.54503561037001	19.762697364266188	46.47753900082621	17.214728024537592
24	17.599055685516714	17.97407302030237	47.32829447194499	17.098576822235923
25	16.093647557927483	19.5171782028987	46.41269225335286	17.976481985820957
26	15.409761779353081	20.98508459700937	46.73887920600496	16.866274417632585
27	16.45616431488695	20.773095631373586	46.25871378169173	16.51202627204773
28	14.637851098585939	20.671463329359625	47.29189956046145	17.398786011592986
29	15.286839430728893	18.938766049732767	47.15823452776788	18.616159991770452
30	15.872869123507662	20.107570076481398	45.29682036083699	18.722740439173947
31	15.838752963190355	21.469026237540927	43.54009853320043	19.15212226606829
32	17.524963719026072	22.395110711497733	41.957733723368726	18.12219184610747
33	18.365757792189612	23.88958083348905	39.01085271519712	18.733808659124218
34	16.98385797781017	24.194152203650038	38.94021142904392	19.881778389495867
35	18.170696692360128	24.04062948222216	36.25343358970369	21.53524023571402
36	18.589791585418336	26.46417900827447	34.46396285244956	20.482066553857635
37	18.50749611472926	26.66952704194009	35.51707142712977	19.30590541620088
38	18.691358780256117	26.69322605406891	33.32706134202818	21.288353823646798
39	19.59634852913123	25.43900141217465	35.09478628243884	19.869863776255283
40	20.218577811865003	24.731221299942902	36.93764490415899	18.112555984033115
41	18.134497102405124	26.066699697707378	35.57833727991333	20.220465919974163
42	20.24045382306083	25.95933796418975	33.5727758249242	20.227432387825218
43	20.353740309610664	26.120022474997217	32.94833289820037	20.577904317191745
44	18.54350038315573	26.586059642079814	33.17360372777648	21.69683624698798
45	18.138143104271094	25.503978374000365	33.32471748368577	23.033161038042774
46	18.209565676538432	26.45877511265169	33.90215302920903	21.42950618160084
47	17.544495871879494	25.296872446577932	36.7642644939968	20.39436718754578
48	18.387438481856908	23.327575688719985	35.64044952598721	22.6445363034359
49	17.937547894466476	23.415014626327128	36.76927774656251	21.878159732643894
50	16.89746075502188	23.45127932345831	36.76465513705386	22.88660478446595
51	16.86952977644149	22.871304598064103	35.53823125938764	24.720934366106768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	494.0
1	883.5
2	1273.0
3	58663.5
4	116054.0
5	82639.5
6	49225.0
7	47289.5
8	45354.0
9	43912.0
10	42470.0
11	40229.5
12	37989.0
13	35338.0
14	32687.0
15	29838.0
16	26989.0
17	25029.5
18	23070.0
19	21343.0
20	19616.0
21	18413.5
22	17211.0
23	15490.5
24	13770.0
25	13873.5
26	14676.5
27	15376.0
28	16957.5
29	18539.0
30	20147.0
31	21755.0
32	23552.5
33	25350.0
34	27222.5
35	29095.0
36	31032.0
37	32969.0
38	35441.5
39	37914.0
40	43132.5
41	48351.0
42	52237.5
43	56124.0
44	61811.0
45	67498.0
46	72027.5
47	76557.0
48	83705.0
49	90853.0
50	98727.5
51	106602.0
52	105379.5
53	104157.0
54	87771.5
55	71386.0
56	63903.0
57	56420.0
58	55192.0
59	53964.0
60	52992.5
61	52021.0
62	47557.0
63	43093.0
64	36013.0
65	28933.0
66	23758.0
67	18583.0
68	16093.0
69	13603.0
70	11691.5
71	9780.0
72	8720.5
73	7661.0
74	5759.5
75	3214.5
76	2571.0
77	1931.5
78	1292.0
79	1101.0
80	910.0
81	590.5
82	271.0
83	203.5
84	136.0
85	107.5
86	79.0
87	58.0
88	37.0
89	22.0
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1535929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.78021743259678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26226358934305	25.326737997932703
2	9.854959536715139	6.46095432805931
3	3.8316231238024736	3.768043173540323
4	1.8974883347398128	2.4880032075434815
5	1.0911506279656376	1.7884077418214055
6	0.7190243144965376	1.414186402111221
7	0.46302867658723734	1.062472648724002
8	0.3603268185064232	0.9449273165949111
9	0.27965862496717975	0.8250543479992669
>10	3.345228081369335	27.429988530939887
>50	0.7927777481771887	16.78153035558227
>100	0.09496278260161614	5.4219677159648585
>500	0.004666973966309539	1.0166147374725703
>1k	0.002637854850522783	1.5892420781491834
>5k	0.0	0.0
>10k+	2.0291191157867562E-4	3.6818694175645548
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55354	3.603942630160639	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4535	0.29526104396752717	No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4281	0.27872382121829853	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2311	0.15046268414750943	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	1825	0.11882059652496958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9532152853419656E-4	0.0	0.0	0.1593823672839044	0.0
2	1.9532152853419656E-4	0.0	0.0	0.9475047349193876	0.0
3	1.9532152853419656E-4	0.0	0.0	1.3735009886524703	0.0
4	1.9532152853419656E-4	0.0	0.0	1.7660321538300272	0.0
5	1.9532152853419656E-4	0.0	0.0	2.8416678114678477	0.0
6	1.9532152853419656E-4	0.0	0.0	3.4166292842963446	0.0
7	1.9532152853419656E-4	0.0	0.0	4.0083884085787815	0.0
8	1.9532152853419656E-4	0.0	0.0	4.922948912352068	0.0
9	1.9532152853419656E-4	0.0	0.0	5.2904789218772486	0.0
10	1.9532152853419656E-4	0.0	0.0	6.065058996867694	0.0
11	1.9532152853419656E-4	0.0	0.0	7.183209640549791	0.0
12	1.9532152853419656E-4	0.0	0.0	8.062286733306031	0.0
13	1.9532152853419656E-4	0.0	0.0	8.413409734434339	0.0
14	1.9532152853419656E-4	0.0	0.0	8.558208094254358	0.0
15	1.9532152853419656E-4	0.0	0.0	8.848911635889419	0.0
16	1.9532152853419656E-4	0.0	0.0	9.440475438643322	0.0
17	1.9532152853419656E-4	0.0	0.0	10.159323770825344	0.0
18	1.9532152853419656E-4	0.0	0.0	10.953891748902455	0.0
19	1.9532152853419656E-4	0.0	0.0	11.397727368908328	0.0
20	2.6042870471226206E-4	0.0	0.0	11.829062411088012	0.0
21	2.6042870471226206E-4	0.0	0.0	12.373618832641352	0.0
22	2.6042870471226206E-4	0.0	0.0	13.000926475117014	0.0
23	2.6042870471226206E-4	0.0	0.0	13.595550315151286	0.0
24	2.6042870471226206E-4	0.0	0.0	14.038279113162131	0.0
25	2.6042870471226206E-4	0.0	0.0	14.427554919530786	0.0
26	2.6042870471226206E-4	0.0	0.0	14.797689216103088	0.0
27	2.6042870471226206E-4	0.0	0.0	15.150439896635847	0.0
28	2.6042870471226206E-4	0.0	0.0	15.526303624711819	0.0
29	2.6042870471226206E-4	0.0	0.0	15.899693280093025	0.0
30	2.6042870471226206E-4	0.0	0.0	16.33708328965727	0.0
31	2.6042870471226206E-4	0.0	0.0	16.764251472561558	0.0
32	2.6042870471226206E-4	0.0	0.0	17.181979114920026	0.0
33	2.6042870471226206E-4	0.0	0.0	17.557061556881862	0.0
34	3.255358808903276E-4	0.0	0.0	17.91547656174211	0.0
35	3.255358808903276E-4	0.0	0.0	18.296548863912328	0.0
36	3.255358808903276E-4	0.0	0.0	18.70613810924854	0.0
37	3.255358808903276E-4	0.0	0.0	19.1348688643811	0.0
38	3.906430570683931E-4	0.0	0.0	19.502008230849214	0.0
39	3.906430570683931E-4	0.0	0.0	19.861464950528312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	30	2.1659798E-6	45.000004	1
TCCGATC	25	3.8914208E-5	45.0	14
TATTGCG	20	7.0340297E-4	45.0	1
ACGATAA	20	7.0340297E-4	45.0	34
CCGTACT	25	3.8914208E-5	45.0	41
ATCGGCG	20	7.0340297E-4	45.0	3
AGTACGT	25	3.8914208E-5	45.0	10
CGTTTTT	42660	0.0	44.50422	1
CGAATAT	205	0.0	43.90244	14
GCTACGA	210	0.0	42.857143	10
ATCCGCC	50	1.0822987E-9	40.5	18
GTTTTTT	47590	0.0	40.05936	2
CGGTCTA	45	1.9290383E-8	40.0	31
AGCTACG	225	0.0	39.0	9
ATTAACG	35	6.24966E-6	38.57143	1
ACGGGTA	245	0.0	38.57143	5
TCTACGG	35	6.24966E-6	38.57143	2
GTATGCG	35	6.24966E-6	38.57143	1
CGAATTA	35	6.24966E-6	38.57143	37
GTCCAAT	35	6.24966E-6	38.57143	44
>>END_MODULE