##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934870.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1447945
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6098726125647	34.0	31.0	34.0	31.0	34.0
2	32.765539436926126	34.0	31.0	34.0	31.0	34.0
3	32.82039234915691	34.0	31.0	34.0	31.0	34.0
4	36.21270075866141	37.0	37.0	37.0	35.0	37.0
5	36.104773316666034	37.0	35.0	37.0	35.0	37.0
6	36.097242643884954	37.0	36.0	37.0	35.0	37.0
7	36.2607999613245	37.0	37.0	37.0	35.0	37.0
8	36.2750180428124	37.0	37.0	37.0	35.0	37.0
9	38.07520589525154	39.0	39.0	39.0	37.0	39.0
10	37.66729606442234	39.0	38.0	39.0	35.0	39.0
11	37.53713918691663	39.0	37.0	39.0	35.0	39.0
12	37.4676172092172	39.0	37.0	39.0	35.0	39.0
13	37.42040339930039	39.0	37.0	39.0	35.0	39.0
14	38.68235050364482	40.0	38.0	41.0	35.0	41.0
15	38.710797716764105	40.0	38.0	41.0	35.0	41.0
16	38.77744872906084	40.0	38.0	41.0	35.0	41.0
17	38.748895158310575	40.0	38.0	41.0	35.0	41.0
18	38.26989699194375	39.0	38.0	41.0	34.0	41.0
19	37.81926523452203	38.0	37.0	41.0	34.0	41.0
20	37.294652075873046	38.0	35.0	41.0	34.0	41.0
21	37.242418738280804	38.0	35.0	41.0	34.0	41.0
22	37.05203996008136	38.0	35.0	40.0	34.0	41.0
23	36.95526280349046	38.0	35.0	40.0	34.0	41.0
24	36.787964321849245	37.0	35.0	40.0	33.0	41.0
25	36.6613856189289	37.0	35.0	40.0	33.0	41.0
26	36.79794950775064	38.0	35.0	40.0	33.0	41.0
27	36.84775319504539	38.0	35.0	40.0	34.0	41.0
28	36.79764839134083	38.0	35.0	40.0	33.0	41.0
29	36.680882215830025	38.0	35.0	40.0	33.0	41.0
30	36.380915711577444	38.0	35.0	40.0	33.0	41.0
31	35.91697889077278	38.0	35.0	40.0	31.0	41.0
32	35.206675667929375	38.0	35.0	40.0	25.0	41.0
33	34.313520195863795	38.0	35.0	40.0	18.0	41.0
34	33.54312076770872	38.0	34.0	41.0	14.0	41.0
35	33.045799391551476	38.0	33.0	41.0	10.0	41.0
36	32.856886138630955	38.0	33.0	41.0	10.0	41.0
37	32.63738401665809	38.0	33.0	40.0	9.0	41.0
38	32.53784570546533	37.0	32.0	40.0	9.0	41.0
39	32.52094658291579	37.0	32.0	40.0	9.0	41.0
40	32.41012607523076	37.0	31.0	40.0	8.0	41.0
41	32.2874114693583	37.0	31.0	40.0	8.0	41.0
42	32.17441822721167	37.0	31.0	40.0	8.0	41.0
43	32.06914834472304	37.0	31.0	40.0	8.0	41.0
44	31.962628414753322	37.0	30.0	40.0	8.0	41.0
45	31.92209994164143	37.0	30.0	40.0	8.0	41.0
46	31.858389648778097	36.0	30.0	40.0	8.0	41.0
47	31.84104644858748	36.0	30.0	40.0	8.0	41.0
48	31.79116955409218	36.0	30.0	40.0	8.0	41.0
49	31.669642838643732	36.0	29.0	40.0	8.0	41.0
50	31.56003991864332	36.0	29.0	40.0	8.0	41.0
51	31.006761997175307	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	11.0
10	15.0
11	6.0
12	14.0
13	22.0
14	27.0
15	52.0
16	97.0
17	207.0
18	420.0
19	825.0
20	1474.0
21	2345.0
22	3865.0
23	6512.0
24	12601.0
25	27625.0
26	51145.0
27	67120.0
28	64961.0
29	53702.0
30	42403.0
31	37028.0
32	36081.0
33	43359.0
34	84446.0
35	101484.0
36	97807.0
37	126294.0
38	247119.0
39	338637.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.00448566761859	16.09246207556226	19.01349844089382	35.88955381592533
2	48.874301164754186	18.533507833515774	19.111292210684798	13.48089879104524
3	22.84575726287946	18.835383940688356	45.75291188546527	12.565946910966922
4	21.646195124814824	19.41227049369969	45.35683330513245	13.58470107635304
5	19.403706632503308	21.812361657383395	43.92141966718349	14.862512042929806
6	20.2465563263798	25.017386710130562	42.826626701981084	11.909430261508552
7	60.20850239477328	4.317774501103288	31.565011101941025	3.908712002182403
8	61.25909478605887	3.9188643215039245	30.56186526421929	4.260175628217923
9	56.855750736388465	5.620931734285487	32.45047291160921	5.072844617716833
10	31.609971373222052	20.9012773275228	37.36868458401389	10.120066715241256
11	24.118250347906862	18.596562714744	42.98333154919558	14.301855388153555
12	23.083335347682404	15.364188556885793	45.87798569697053	15.674490398461268
13	19.409853274813614	17.396171815918425	47.14598966120951	16.047985248058456
14	15.437326694038791	20.12355441677688	46.444650867263604	17.994468021920724
15	15.812340938364372	19.092023522992932	50.52933640435238	14.566299134290322
16	17.64473098080383	18.651606241949796	47.97606262668817	15.727600150558205
17	16.896567203864787	19.37718628815321	46.024192907879794	17.702053600102214
18	17.320340206292368	19.931696300619155	47.18224794450065	15.565715548587827
19	17.928926858409678	21.157985973224122	45.1276809547324	15.785406213633804
20	20.34214006747494	20.612937646112247	45.270711249391375	13.774211037021434
21	18.93504242219145	21.134780671917788	45.35434702284962	14.57582988304114
22	16.920877519519042	19.97189119752477	45.22133092071867	17.885900362237518
23	17.15313772277262	19.71331784011133	46.19022131365487	16.94332312346118
24	19.039811595053678	17.07191916820045	46.72028288367307	17.167986353072802
25	16.601459309573222	19.213713228057696	44.7779439136155	19.40688354875358
26	15.232277469102762	20.504991556999748	46.47407187427699	17.788659099620496
27	15.551143171874623	21.032428717941634	45.91928560822407	17.497142501959672
28	13.549685934203302	22.0915849704236	47.19592249705617	17.162806598316923
29	16.00053869449461	20.582204434560705	46.07094882747618	17.346308043468504
30	17.786034690544188	21.004941486037108	45.12422778489514	16.084796038523564
31	18.055796318230318	21.334857332288173	43.753043105919076	16.85630324356243
32	18.45215115215012	23.700278670805865	41.68114120356782	16.1664289734762
33	19.226559019852274	23.258549185224577	39.68438027687516	17.83051151804799
34	17.82636771424329	23.993936233765787	40.33081367040875	17.84888238158217
35	16.806508534509256	26.754469265061864	37.533124531663844	18.905897668765043
36	18.133630766361982	28.561858357879615	35.27012421051904	18.034386665239357
37	18.72467531570605	28.79695016039974	36.200270037881275	16.278104486012936
38	18.435299683344326	28.55322543328648	34.16580049656582	18.845674386803367
39	20.522602723169733	25.361805869698088	34.810990748958005	19.30460065817417
40	20.768399352185337	24.33807913974633	35.50770229532199	19.38581921274634
41	17.048437613307136	26.81586662476821	34.600830832662844	21.534864929261815
42	17.34071390833215	26.42199807313123	34.58301247630262	21.654275542233993
43	18.646219297003686	25.687923229128177	34.93350921478371	20.732348259084425
44	18.251107604225297	25.371681935432633	34.61422913163138	21.76298132871069
45	18.619906142843824	24.802737673046973	34.39909665077058	22.17825953333863
46	18.981383961407374	25.724803082990032	35.10906836930961	20.18474458629299
47	16.8211499746192	24.95046427868462	37.90447841596193	20.323907330734247
48	16.66334011305678	23.346328762487527	37.97575184140282	22.01457928305288
49	17.839075379244377	22.345600143651865	38.81853247188257	20.99679200522119
50	17.56323617264468	22.014233966069153	38.171339380984776	22.251190480301393
51	16.266847152343495	22.142968137601912	37.14726733404929	24.442917376005305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	495.0
1	797.0
2	1099.0
3	60966.5
4	120834.0
5	81322.0
6	41810.0
7	39201.5
8	36593.0
9	34990.5
10	33388.0
11	31707.5
12	30027.0
13	28137.5
14	26248.0
15	23895.5
16	21543.0
17	19886.5
18	18230.0
19	16986.5
20	15743.0
21	14360.0
22	12977.0
23	12177.0
24	11377.0
25	11521.0
26	12972.5
27	14280.0
28	14939.0
29	15598.0
30	17369.5
31	19141.0
32	21670.5
33	24200.0
34	27076.5
35	29953.0
36	31243.0
37	32533.0
38	34712.0
39	36891.0
40	43790.5
41	50690.0
42	57671.0
43	64652.0
44	80412.5
45	96173.0
46	104649.0
47	113125.0
48	113347.0
49	113569.0
50	96519.5
51	79470.0
52	71693.5
53	63917.0
54	59708.0
55	55499.0
56	53134.0
57	50769.0
58	49916.0
59	49063.0
60	49570.5
61	50078.0
62	45262.0
63	40446.0
64	34782.0
65	29118.0
66	22145.5
67	15173.0
68	12043.5
69	8914.0
70	7031.0
71	5148.0
72	4184.0
73	3220.0
74	2619.5
75	1558.5
76	1098.0
77	832.5
78	567.0
79	419.0
80	271.0
81	234.5
82	198.0
83	143.0
84	88.0
85	49.0
86	10.0
87	12.5
88	15.0
89	18.5
90	22.0
91	14.5
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1447945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.07659788246135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20398640770438	24.12288031158022
2	10.568815772483543	6.780233072555395
3	4.038311718481957	3.8860590335333165
4	2.1213556970661362	2.7218349464183578
5	1.2064177391928137	1.934888834917801
6	0.7660420159896425	1.4743213024781878
7	0.5591665887538159	1.2555313271734652
8	0.4092324246848206	1.050142714166371
9	0.3295252401183694	0.9513043757458612
>10	4.163759650587914	32.95521629720273
>50	0.5493891334082124	11.005643312109484
>100	0.07520205027243256	4.736144590953911
>500	0.004617669659414529	0.999522014847244
>1k	0.0037381135338117612	1.883511106095604
>5k	2.1988903140069186E-4	0.37156742461472597
>10k+	2.1988903140069186E-4	3.8711993356072956
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54885	3.790544530351636	No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	5268	0.36382597405288186	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	4609	0.3183131955978991	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2530	0.17473039376495653	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1715	0.11844372541774723	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	1664	0.11492149218375008	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1505	0.10394041210128838	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.90633967450421E-5	0.0	0.0	0.1850208398799678	0.0
2	6.90633967450421E-5	0.0	0.0	1.1247664793897558	0.0
3	6.90633967450421E-5	0.0	0.0	1.571330402743198	0.0
4	6.90633967450421E-5	0.0	0.0	2.028530089195377	0.0
5	6.90633967450421E-5	0.0	0.0	3.2907327281077667	0.0
6	6.90633967450421E-5	0.0	0.0	3.9335057616138736	0.0
7	6.90633967450421E-5	0.0	0.0	4.664956196540614	0.0
8	6.90633967450421E-5	0.0	0.0	5.756779435682986	0.0
9	6.90633967450421E-5	0.0	0.0	6.1811740086812685	0.0
10	6.90633967450421E-5	0.0	0.0	7.148614070285819	0.0
11	6.90633967450421E-5	0.0	0.0	8.433193249743603	0.0
12	1.381267934900842E-4	0.0	0.0	9.539036358425216	0.0
13	1.381267934900842E-4	0.0	0.0	9.962394980472325	0.0
14	1.381267934900842E-4	0.0	0.0	10.141890748612688	0.0
15	1.381267934900842E-4	0.0	0.0	10.448255976573696	0.0
16	1.381267934900842E-4	0.0	0.0	11.077216330730794	0.0
17	1.381267934900842E-4	0.0	0.0	11.836982758323003	0.0
18	1.381267934900842E-4	0.0	0.0	12.69316168777129	0.0
19	1.381267934900842E-4	0.0	0.0	13.26583537358118	0.0
20	1.381267934900842E-4	0.0	0.0	13.810745573899561	0.0
21	1.381267934900842E-4	0.0	0.0	14.461529961428093	0.0
22	2.0719019023512634E-4	0.0	0.0	15.131237719664766	0.0
23	2.0719019023512634E-4	0.0	0.0	15.774563260344834	0.0
24	2.762535869801684E-4	0.0	0.0	16.31470808628781	0.0
25	2.762535869801684E-4	0.0	0.0	16.777778161463317	0.0
26	2.762535869801684E-4	0.0	0.0	17.208526566962142	0.0
27	2.762535869801684E-4	0.0	0.0	17.62339039120961	0.0
28	2.762535869801684E-4	0.0	0.0	18.045851189099032	0.0
29	2.762535869801684E-4	0.0	0.0	18.47563270704343	0.0
30	2.762535869801684E-4	0.0	0.0	18.951962954393984	0.0
31	2.762535869801684E-4	0.0	0.0	19.410267655194087	0.0
32	2.762535869801684E-4	0.0	0.0	19.870575194499793	0.0
33	2.762535869801684E-4	0.0	0.0	20.304707706439125	0.0
34	2.762535869801684E-4	0.0	0.0	20.73759707723705	0.0
35	2.762535869801684E-4	0.0	0.0	21.17014113105125	0.0
36	2.762535869801684E-4	0.0	0.0	21.64439947649945	0.0
37	2.762535869801684E-4	0.0	0.0	22.105052332788883	0.0
38	2.762535869801684E-4	0.0	0.0	22.57482155744866	0.0
39	2.762535869801684E-4	0.0	0.0	23.043969211537732	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAC	20	7.0338935E-4	45.000004	36
TTAGCCG	20	7.0338935E-4	45.000004	1
TGACGTA	25	3.8913087E-5	45.000004	26
TGACGCG	25	3.8913087E-5	45.000004	13
ACTAACG	20	7.0338935E-4	45.000004	1
GTATACG	50	2.1827873E-11	45.000004	1
TCGGTAA	20	7.0338935E-4	45.000004	13
TAATCCG	20	7.0338935E-4	45.000004	43
ATTAGCG	35	1.2122291E-7	45.0	1
TGTAGCG	70	0.0	45.0	1
TTACGCG	30	2.1658943E-6	44.999996	1
CGTTTTT	36930	0.0	44.5065	1
TACGCGG	100	0.0	42.750004	2
GTAAGCG	50	1.0822987E-9	40.500004	1
GTCGAAG	45	1.9288564E-8	40.0	1
TATAGCG	45	1.9288564E-8	40.0	1
ATCGCAT	45	1.9288564E-8	40.0	10
GTTTTTT	41280	0.0	39.969116	2
GCTAGCG	80	0.0	39.375004	1
TATACGG	105	0.0	38.57143	2
>>END_MODULE