##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934869.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474613
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44424194027555	34.0	31.0	34.0	30.0	34.0
2	32.62979100867444	34.0	31.0	34.0	31.0	34.0
3	32.6924336248691	34.0	31.0	34.0	31.0	34.0
4	36.11957321017334	37.0	37.0	37.0	35.0	37.0
5	35.97337198938925	37.0	35.0	37.0	35.0	37.0
6	35.972937951552105	37.0	35.0	37.0	35.0	37.0
7	36.151529772677954	37.0	36.0	37.0	35.0	37.0
8	36.16753228419786	37.0	37.0	37.0	35.0	37.0
9	37.9711322698704	39.0	39.0	39.0	35.0	39.0
10	37.49209355833068	39.0	37.0	39.0	34.0	39.0
11	37.39298122891703	39.0	37.0	39.0	34.0	39.0
12	37.36604981321624	39.0	37.0	39.0	35.0	39.0
13	37.23722274779663	39.0	37.0	39.0	34.0	39.0
14	38.433176082408195	40.0	38.0	41.0	34.0	41.0
15	38.50367984020665	40.0	38.0	41.0	34.0	41.0
16	38.606940812830665	40.0	38.0	41.0	34.0	41.0
17	38.627690349821854	40.0	38.0	41.0	35.0	41.0
18	38.19704264316401	39.0	38.0	40.0	35.0	41.0
19	37.73841635184877	38.0	37.0	40.0	34.0	41.0
20	37.2088333020798	38.0	35.0	40.0	34.0	41.0
21	37.13848335380615	38.0	35.0	40.0	34.0	41.0
22	36.943079940920285	38.0	35.0	40.0	33.0	41.0
23	36.80718395829866	37.0	35.0	40.0	33.0	41.0
24	36.61432367002168	37.0	35.0	40.0	33.0	41.0
25	36.47911666979202	37.0	35.0	40.0	33.0	41.0
26	36.6581446357348	37.0	35.0	40.0	33.0	41.0
27	36.738273077222914	38.0	35.0	40.0	33.0	41.0
28	36.722000872289634	38.0	35.0	40.0	33.0	41.0
29	36.60716204570882	38.0	35.0	40.0	33.0	41.0
30	36.31520206989695	38.0	35.0	40.0	33.0	41.0
31	35.85414010994221	38.0	35.0	40.0	31.0	41.0
32	35.11693105751423	38.0	35.0	40.0	25.0	41.0
33	34.20408416962873	38.0	34.0	40.0	18.0	41.0
34	33.419120420216046	38.0	34.0	40.0	12.0	41.0
35	32.923010958401896	38.0	33.0	40.0	10.0	41.0
36	32.72771078752584	38.0	33.0	40.0	9.0	41.0
37	32.499904132419466	37.0	33.0	40.0	8.0	41.0
38	32.35100176354209	37.0	31.0	40.0	8.0	41.0
39	32.31568246128951	37.0	31.0	40.0	8.0	41.0
40	32.17067379106767	37.0	31.0	40.0	8.0	41.0
41	32.06914686281244	37.0	31.0	40.0	8.0	41.0
42	31.93675268060504	37.0	31.0	40.0	7.0	41.0
43	31.781343958130098	37.0	30.0	40.0	7.0	41.0
44	31.631533480962386	37.0	30.0	40.0	7.0	41.0
45	31.59775227395794	37.0	29.0	40.0	7.0	41.0
46	31.529216435285168	37.0	29.0	40.0	7.0	41.0
47	31.48868867898688	36.0	29.0	40.0	7.0	41.0
48	31.422721248680503	36.0	29.0	40.0	7.0	41.0
49	31.33891402047563	36.0	28.0	40.0	7.0	41.0
50	31.221142278024413	36.0	27.0	40.0	7.0	41.0
51	30.57465345449872	35.0	25.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	5.0
11	2.0
12	4.0
13	8.0
14	5.0
15	19.0
16	29.0
17	68.0
18	164.0
19	300.0
20	588.0
21	877.0
22	1628.0
23	2754.0
24	5505.0
25	11249.0
26	19551.0
27	23286.0
28	19774.0
29	14810.0
30	11585.0
31	10749.0
32	11437.0
33	14190.0
34	25709.0
35	39357.0
36	33948.0
37	45966.0
38	86971.0
39	94019.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.497112384195123	17.265013811252537	19.846274754378832	34.39159905017351
2	47.28041583353174	19.30288466603317	19.845642660441243	13.571056839993847
3	22.471992971115416	19.78032628689058	45.28215619883989	12.465524543154107
4	21.779007317540817	20.108593738477452	45.0771470650825	13.03525187889923
5	18.390141020157476	22.55479727693932	43.98214966720254	15.072912035700666
6	19.065849439438026	24.82675358660635	43.65198593380291	12.455411040152715
7	58.14463573479867	5.095519928868362	32.22878429372984	4.531060042603132
8	58.45246548240356	4.07152774997735	31.454890616144098	6.021116151474992
9	54.312882285146	5.694534283721684	33.91415742931609	6.078426001816217
10	29.05630482098046	19.463436526180278	39.99595459879944	11.484304054039818
11	22.089997534833643	20.13366679800174	43.10691026162368	14.669425405540936
12	20.630071236986765	16.585723526325662	47.3750192261906	15.409186010496972
13	16.467311262017688	22.635915998929654	47.386607615046366	13.510165124006296
14	12.83761717441368	25.570517453167106	45.15447322344731	16.4373921489719
15	11.912442347765442	21.213915337337998	52.59864352640994	14.27499878848662
16	13.02008162439714	20.757754212379346	48.729385836460445	17.492778326763066
17	12.940227090281978	20.241333465370733	44.82367739611009	21.994762048237195
18	14.243815487565659	20.031267580112637	48.346758306241085	17.378158626080616
19	15.993030110848208	21.017966216686016	45.62706879078323	17.36193488168255
20	17.248157972916882	21.383527210590522	45.07756846104089	16.290746355451706
21	15.94119840796607	22.18101906184618	46.07016664103175	15.807615889156008
22	14.460623708158016	21.310204313830425	43.47373544340336	20.755436534608197
23	13.326647184126857	22.938056901096264	44.194533230231784	19.540762684545093
24	15.943937481695613	19.972693541896238	45.719775901629326	18.36359307477882
25	12.875964206627295	21.629411752311885	45.38286983289544	20.11175420816539
26	12.662316455722872	22.752010585466476	47.191079890352775	17.39459306845788
27	14.762975308303819	22.992627677707944	45.81332580439221	16.43107120959603
28	13.640797871107619	21.36372160054613	47.7704993331409	17.22498119520536
29	13.21961261069545	19.08418016362805	49.46303620001981	18.233171025656695
30	14.799847454663063	22.017938825948722	46.69509684732614	16.48711687206208
31	16.782936834852816	22.473889252928174	41.71040405551471	19.032769856704306
32	16.406419546030136	23.872081042870718	42.593649984303	17.12784942679615
33	18.041646562567816	23.75682924825068	39.72267931978264	18.47884486939886
34	15.839852679972946	22.649611367577375	40.35266627757773	21.15786967487195
35	15.714487382351516	23.53833544382476	39.257247483739384	21.48992969008434
36	20.664415007595665	24.351208247561697	37.12666951811266	17.85770722672999
37	18.430595032163048	24.54884295204725	38.940779118987464	18.07978289680224
38	18.13182529766357	28.327921906900993	34.17036617201805	19.369886623417397
39	19.403808998067902	25.101293053498324	36.03377910002465	19.461118848409125
40	19.6819303306062	24.708341322298377	35.22217048416289	20.38755786293254
41	18.121501096682984	24.942216079205583	33.02164078944319	23.914642034668248
42	20.559276715977017	23.416762709828852	34.68594412710988	21.338016447084257
43	21.51732042737978	23.092498519846696	35.662950656640255	19.727230396133272
44	18.792152764462834	23.827413071281235	35.08879866333202	22.291635500923913
45	18.03132236158723	23.55877314780674	34.607985874807476	23.801918615798556
46	20.096373255684107	24.055598982750155	35.16486063382166	20.683167127744078
47	16.524410414379716	23.54697406097178	37.86959059275662	22.059024931891877
48	17.763735928008714	21.917225191893184	36.9490511216507	23.369987758447408
49	19.432674621217707	20.023682452861593	38.96458799063659	21.579054935284113
50	18.27868178916296	20.4625663435262	37.87001198871502	23.388739878595825
51	16.494701999313126	21.449264980099574	33.77657164890132	28.279461371685983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	652.0
1	613.5
2	575.0
3	13698.0
4	26821.0
5	18410.0
6	9999.0
7	10205.0
8	10411.0
9	10835.0
10	11259.0
11	11257.0
12	11255.0
13	10679.0
14	10103.0
15	9516.5
16	8930.0
17	8237.0
18	7544.0
19	7003.5
20	6463.0
21	5858.0
22	5253.0
23	5069.0
24	4885.0
25	4464.0
26	4139.0
27	4235.0
28	4675.5
29	5116.0
30	5264.0
31	5412.0
32	5539.0
33	5666.0
34	7021.0
35	8376.0
36	8569.0
37	8762.0
38	10647.0
39	12532.0
40	16002.5
41	19473.0
42	24273.0
43	29073.0
44	36596.5
45	44120.0
46	49801.5
47	55483.0
48	53287.0
49	51091.0
50	41082.5
51	31074.0
52	25911.5
53	20749.0
54	17797.0
55	14845.0
56	13048.5
57	11252.0
58	9915.5
59	8579.0
60	7614.0
61	6649.0
62	5834.0
63	5019.0
64	4225.5
65	3432.0
66	2748.0
67	2064.0
68	1676.0
69	1288.0
70	1094.5
71	901.0
72	737.5
73	574.0
74	413.5
75	207.5
76	162.0
77	126.5
78	91.0
79	91.0
80	91.0
81	68.5
82	46.0
83	26.0
84	6.0
85	4.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	474613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.62950171788469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.39842246761384	29.512329919563697
2	10.444791769035202	8.069542031697866
3	4.23166771175973	4.90401645422819
4	2.1051939378094233	3.2529037134835828
5	1.215246870508976	2.3472190535990243
6	0.8022461062127837	1.8594220402867818
7	0.5208736427079901	1.4084762493052412
8	0.3870545696178744	1.1961380129575039
9	0.3078267053622684	1.070207301924228
>10	3.2187527515779504	28.826416357355413
>50	0.31067857778975516	7.535307442817733
>100	0.051131358061999005	3.588170074984689
>500	0.003334653786652109	0.9609674657830114
>1k	0.0022231025244347394	2.1291140210389017
>5k	0.0	0.0
>10k+	5.557756311086848E-4	3.339769860974201
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15556	3.2776177643680215	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	3380	0.7121591696814036	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	3194	0.6729693455510068	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	1928	0.40622570388927404	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1415	0.29813764056188935	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCC	953	0.2007951741734845	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	944	0.1988988923607234	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	913	0.19236725500565724	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	580	0.12220482793349528	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	566	0.11925505622475574	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	520	0.10956294918175441	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1069797919568152E-4	0.0	0.0	0.43087736745516875	0.0
2	2.1069797919568152E-4	0.0	0.0	2.4232374587295333	0.0
3	2.1069797919568152E-4	0.0	0.0	3.3096438572057654	0.0
4	2.1069797919568152E-4	0.0	0.0	4.227233556602958	0.0
5	2.1069797919568152E-4	0.0	0.0	6.4673744714114445	0.0
6	2.1069797919568152E-4	0.0	0.0	7.674463194223504	0.0
7	2.1069797919568152E-4	0.0	0.0	8.971098558193729	0.0
8	2.1069797919568152E-4	0.0	0.0	10.948709790924395	0.0
9	2.1069797919568152E-4	0.0	0.0	11.687838301942847	0.0
10	2.1069797919568152E-4	0.0	0.0	13.212659577381993	0.0
11	2.1069797919568152E-4	0.0	0.0	15.38600923278545	0.0
12	2.1069797919568152E-4	0.0	0.0	17.111836380377277	0.0
13	2.1069797919568152E-4	0.0	0.0	17.74793357956904	0.0
14	2.1069797919568152E-4	0.0	0.0	17.974433907204396	0.0
15	2.1069797919568152E-4	0.0	0.0	18.43101642812144	0.0
16	2.1069797919568152E-4	0.0	0.0	19.422561118216315	0.0
17	2.1069797919568152E-4	0.0	0.0	20.68506340955684	0.0
18	2.1069797919568152E-4	0.0	0.0	22.324082989720047	0.0
19	2.1069797919568152E-4	0.0	0.0	23.110197150099133	0.0
20	2.1069797919568152E-4	0.0	0.0	23.83015214501078	0.0
21	2.1069797919568152E-4	0.0	0.0	24.65250635781152	0.0
22	2.1069797919568152E-4	0.0	0.0	25.432931672752325	0.0
23	2.1069797919568152E-4	0.0	0.0	26.29721478341301	0.0
24	2.1069797919568152E-4	0.0	0.0	26.902760775621402	0.0
25	2.1069797919568152E-4	0.0	0.0	27.45352529323891	0.0
26	2.1069797919568152E-4	0.0	0.0	27.95856834937096	0.0
27	2.1069797919568152E-4	0.0	0.0	28.48173143171384	0.0
28	2.1069797919568152E-4	0.0	0.0	28.96760097173908	0.0
29	2.1069797919568152E-4	0.0	0.0	29.478543571288608	0.0
30	2.1069797919568152E-4	0.0	0.0	30.072922570599626	0.0
31	4.2139595839136304E-4	0.0	0.0	30.622001504383572	0.0
32	4.2139595839136304E-4	0.0	0.0	31.13083712414114	0.0
33	4.2139595839136304E-4	0.0	0.0	31.581941497599097	0.0
34	4.2139595839136304E-4	0.0	0.0	32.02503934784762	0.0
35	4.2139595839136304E-4	0.0	0.0	32.52776472620851	0.0
36	4.2139595839136304E-4	0.0	0.0	33.00394215919075	0.0
37	4.2139595839136304E-4	0.0	0.0	33.47948749823541	0.0
38	4.2139595839136304E-4	0.0	0.0	33.93290954946451	0.0
39	4.2139595839136304E-4	0.0	0.0	34.36378691691968	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGGC	35	1.209919E-7	45.000004	4
ACACGAC	30	2.1627984E-6	45.000004	26
CCGATAG	35	1.209919E-7	45.000004	11
GACACGA	30	2.1627984E-6	45.000004	25
TCGGGCA	30	2.1627984E-6	45.000004	5
GAACGTA	30	2.1627984E-6	45.000004	10
GCGATCG	35	1.209919E-7	45.000004	9
CGTGTTT	35	1.209919E-7	45.000004	1
TTAGTAG	30	2.1627984E-6	45.000004	1
TAGTGCG	35	1.209919E-7	45.000004	1
GTACGTT	30	2.1627984E-6	45.000004	14
CGGACGG	35	1.209919E-7	45.000004	2
GTGTTGA	20	7.029097E-4	45.0	9
GTTGACT	25	3.887334E-5	45.0	20
CTCACGG	25	3.887334E-5	45.0	32
TCGCAAA	20	7.029097E-4	45.0	13
GATAATA	40	6.8012014E-9	45.0	9
TACCAAT	25	3.887334E-5	45.0	12
ACGCAGA	20	7.029097E-4	45.0	14
CGGTTTA	20	7.029097E-4	45.0	22
>>END_MODULE