##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934867.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1136208 Sequences flagged as poor quality 0 Sequence length 51 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5825720290651 34.0 31.0 34.0 31.0 34.0 2 32.74544361595764 34.0 31.0 34.0 31.0 34.0 3 32.795764507907 34.0 31.0 34.0 31.0 34.0 4 36.19864144593244 37.0 37.0 37.0 35.0 37.0 5 36.08057767591849 37.0 35.0 37.0 35.0 37.0 6 36.070556623435145 37.0 35.0 37.0 35.0 37.0 7 36.22089177333728 37.0 37.0 37.0 35.0 37.0 8 36.21758956106628 37.0 37.0 37.0 35.0 37.0 9 37.98108356920564 39.0 39.0 39.0 35.0 39.0 10 37.594155295509275 39.0 38.0 39.0 35.0 39.0 11 37.46666543449791 39.0 37.0 39.0 35.0 39.0 12 37.482539288580966 39.0 37.0 39.0 35.0 39.0 13 37.49482225085548 39.0 37.0 39.0 35.0 39.0 14 38.80584188810499 40.0 38.0 41.0 35.0 41.0 15 38.841646071846 40.0 38.0 41.0 35.0 41.0 16 38.9226541267092 40.0 38.0 41.0 35.0 41.0 17 38.906428224409616 40.0 38.0 41.0 35.0 41.0 18 38.41595816963091 39.0 38.0 41.0 35.0 41.0 19 37.932602129187615 38.0 37.0 41.0 35.0 41.0 20 37.3504367158126 38.0 35.0 41.0 34.0 41.0 21 37.309194267246845 38.0 35.0 41.0 34.0 41.0 22 37.13103322631068 38.0 35.0 41.0 34.0 41.0 23 37.00658154140791 38.0 35.0 40.0 33.0 41.0 24 36.82080833790996 38.0 35.0 40.0 33.0 41.0 25 36.68576616253362 38.0 35.0 40.0 33.0 41.0 26 36.816944608733614 38.0 35.0 40.0 33.0 41.0 27 36.839735330150816 38.0 35.0 40.0 33.0 41.0 28 36.709603347274445 38.0 35.0 40.0 33.0 41.0 29 36.59143924351879 38.0 35.0 40.0 33.0 41.0 30 36.323791946545 38.0 35.0 40.0 33.0 41.0 31 35.802246595693745 38.0 35.0 40.0 30.0 41.0 32 35.07528287074198 38.0 35.0 40.0 23.0 41.0 33 34.153266831425235 38.0 34.0 40.0 17.0 41.0 34 33.35003450072522 38.0 33.0 41.0 12.0 41.0 35 32.885520960950814 38.0 33.0 41.0 10.0 41.0 36 32.627866552603045 38.0 32.0 41.0 9.0 41.0 37 32.36818522664864 38.0 31.0 40.0 8.0 41.0 38 32.192161998507316 37.0 31.0 40.0 8.0 41.0 39 32.164911706307294 38.0 31.0 40.0 8.0 41.0 40 32.09919310548773 38.0 30.0 40.0 8.0 41.0 41 31.953354491431146 37.0 30.0 40.0 7.0 41.0 42 31.810924584231056 37.0 29.0 40.0 7.0 41.0 43 31.66600921662231 37.0 29.0 40.0 7.0 41.0 44 31.553852815681637 37.0 27.0 40.0 7.0 41.0 45 31.52778804585076 37.0 27.0 40.0 7.0 41.0 46 31.5149074817287 37.0 27.0 40.0 7.0 41.0 47 31.490565107797163 36.0 27.0 40.0 7.0 41.0 48 31.43831323138017 36.0 27.0 40.0 7.0 41.0 49 31.30310119273936 36.0 27.0 40.0 7.0 41.0 50 31.2120078365933 36.0 26.0 40.0 7.0 41.0 51 30.692521967808712 35.0 24.0 40.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 3.0 10 10.0 11 8.0 12 10.0 13 22.0 14 22.0 15 58.0 16 86.0 17 194.0 18 388.0 19 773.0 20 1374.0 21 2297.0 22 3674.0 23 6259.0 24 11222.0 25 23607.0 26 43145.0 27 56406.0 28 53609.0 29 42649.0 30 33944.0 31 29118.0 32 28150.0 33 32874.0 34 53548.0 35 71493.0 36 78673.0 37 103143.0 38 195298.0 39 263991.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.546093672989453 16.050142227479476 18.553381071071495 36.850383028459575 2 50.385932857364146 18.29312942700632 18.328686296875222 12.992251418754314 3 22.73404165434498 18.675629814259363 45.82858068241026 12.761747848985397 4 21.346091560700152 18.490540464422008 46.22357878134989 13.939789193527947 5 19.67676692999873 21.61901694056018 44.32938335234394 14.374832777097152 6 19.639273794938955 24.74001239209722 43.90947784208525 11.71123597087857 7 58.86378198358047 4.548198921324264 32.83650528776421 3.751513807331052 8 59.31774815878783 4.565801332150452 31.977067579175646 4.139382929886077 9 54.34858758255531 6.098091194570008 33.82285637840959 5.730464844465098 10 27.96072550096461 23.299343078027967 38.88038105698956 9.859550364017856 11 20.6622379001028 18.938433807894327 46.18203709180009 14.21729120020278 12 19.61260614253728 16.180840127863913 48.90275372114965 15.303800008449157 13 17.70001619421796 18.0910537507217 49.51135707546506 14.697572979595286 14 14.199952825538986 21.320039992677398 47.835079492487296 16.644927689296328 15 13.540302479827638 19.757914043907455 51.490572148761494 15.211211327503415 16 15.624515933702279 19.541316378691224 48.76202244659428 16.07214524101221 17 15.213939701181472 19.700618196668216 47.245046681593514 17.8403954205568 18 15.853611310605102 19.684335966653993 48.05009294072916 16.411959782011746 19 17.175464351597594 19.720332896793543 46.910600875895966 16.193601875712897 20 17.992832298311576 20.340025769929447 47.009966485009784 14.657175446749187 21 17.204860377677328 20.50707264866996 47.21846704124597 15.069599932406744 22 16.13375367890386 19.371893174489177 46.36571824877135 18.128634897835607 23 15.46442200723811 20.00082731330883 47.3115837945165 17.22316688493656 24 16.438979482629943 18.71153873234478 47.69408418176953 17.155397603255743 25 15.867781251320181 20.362380831678706 46.20263191246673 17.56720600453438 26 15.584822497289228 20.983393885626576 46.338610536099026 17.09317308098517 27 17.22932772872573 20.420820835621647 46.5021369326743 15.847714502978327 28 14.892695703603565 20.62483277709715 48.15130680297974 16.331164716319545 29 16.709528537028433 19.856751580696493 46.682561643642714 16.751158238632364 30 17.0298044020109 20.228250461183165 46.6646071846 16.077337952205934 31 17.02170729303085 21.02995226226184 45.1378620815907 16.810478363116612 32 17.941609282807374 22.929692450678044 42.592905524340615 16.535792742173967 33 17.63295100897019 23.1403933082675 41.66376226888035 17.562893413881966 34 17.0750425978342 23.61926689479391 41.51414177685776 17.79154873051413 35 18.435532930590174 24.197682114542406 37.655077239378706 19.711707715488714 36 19.171049666962386 26.233664962753302 37.05342683733964 17.54185853294467 37 18.216206891695887 26.72468421274978 38.23991733907876 16.819191556475573 38 17.22017447509611 27.0624744765043 36.339561066283636 19.37778998211595 39 18.410273471054595 26.39164659991832 37.00783659329982 18.19024333572726 40 19.09307098700238 24.618379733288272 38.004485094278515 18.284064185430836 41 17.506917747454693 26.248186951684904 35.56373480911946 20.68116049174095 42 18.90525326348697 26.06151338487319 35.16627237266416 19.86696097897568 43 19.464745891597314 26.310675510117864 34.98769591483249 19.236882683452325 44 18.492124681396362 25.530536662301266 36.11680255727824 19.860536099024124 45 18.128106825510823 24.930998549561348 35.96894230633828 20.971952318589555 46 19.207750693534987 24.68720515961866 36.7616668778956 19.343377268950757 47 16.81778336360948 24.42501725036261 39.28998915691493 19.46721022911298 48 17.16648712207624 23.373889287876867 38.50474560995874 20.954877980088153 49 17.6546019742864 23.165916803965473 39.402732598256655 19.776748623491475 50 17.127321757987975 22.651574359624295 39.01310323461901 21.208000647768717 51 16.644135580809145 22.366943376564855 37.39376945066396 23.595151591962036 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 334.0 1 629.5 2 925.0 3 47017.0 4 93109.0 5 64355.5 6 35602.0 7 33283.5 8 30965.0 9 29817.5 10 28670.0 11 26991.0 12 25312.0 13 23697.5 14 22083.0 15 20165.0 16 18247.0 17 16885.0 18 15523.0 19 14412.0 20 13301.0 21 12307.0 22 11313.0 23 11037.5 24 10762.0 25 10582.0 26 11458.5 27 12515.0 28 13500.0 29 14485.0 30 15654.5 31 16824.0 32 18977.5 33 21131.0 34 22456.5 35 23782.0 36 24722.0 37 25662.0 38 28756.5 39 31851.0 40 37294.5 41 42738.0 42 48494.0 43 54250.0 44 67489.5 45 80729.0 46 86307.0 47 91885.0 48 89316.0 49 86747.0 50 75142.5 51 63538.0 52 55507.5 53 47477.0 54 43607.5 55 39738.0 56 37504.5 57 35271.0 58 34001.5 59 32732.0 60 30977.5 61 29223.0 62 25981.0 63 22739.0 64 19349.0 65 15959.0 66 13014.5 67 10070.0 68 8705.5 69 7341.0 70 6048.5 71 4756.0 72 4186.5 73 3617.0 74 2898.5 75 1601.0 76 1022.0 77 882.0 78 742.0 79 559.5 80 377.0 81 264.5 82 152.0 83 113.5 84 75.0 85 47.5 86 20.0 87 18.0 88 16.0 89 15.0 90 14.0 91 8.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1136208.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.93821021223608 #Duplication Level Percentage of deduplicated Percentage of total 1 77.60649273354329 27.114319569589075 2 9.694002373376556 6.773821854378912 3 3.493663950635521 3.6618709655464508 4 1.6688743612379597 2.332299330029722 5 1.0196729549468846 1.7812774023833091 6 0.6546333390615229 1.3723030327241705 7 0.4561316662812423 1.1155496828694094 8 0.36082777937062616 1.0085341444852223 9 0.3004416411576072 0.9447203893746312 >10 4.417032001665453 37.14020429136185 >50 0.2527797322081111 5.451503351948939 >100 0.06877524158725086 4.34213663648844 >500 0.0038493605353666503 0.9353315035082275 >1k 0.0025662403569111005 1.8054802996688248 >5k 0.0 0.0 >10k+ 2.5662403569111E-4 4.220647545642859 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47074 4.143079436159577 No Hit CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC 3798 0.33426978158928644 No Hit GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC 3414 0.3004731528030079 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG 2014 0.17725627701970062 No Hit CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT 1987 0.1748799515581654 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC 1979 0.17417585512511793 No Hit GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC 1891 0.16643079436159575 No Hit CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG 1487 0.13087392449269852 No Hit TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC 1270 0.1117753087462859 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1164 0.10244603100840691 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.801205413093377E-5 0.0 0.0 0.18614549448692494 0.0 2 8.801205413093377E-5 0.0 0.0 1.075155253263487 0.0 3 8.801205413093377E-5 0.0 0.0 1.5461957669722446 0.0 4 8.801205413093377E-5 0.0 0.0 2.0080830270513848 0.0 5 1.7602410826186754E-4 0.0 0.0 3.274752510103784 0.0 6 1.7602410826186754E-4 0.0 0.0 3.892597130102939 0.0 7 1.7602410826186754E-4 0.0 0.0 4.5854280202216495 0.0 8 1.7602410826186754E-4 0.0 0.0 5.623002126371228 0.0 9 1.7602410826186754E-4 0.0 0.0 6.029177756185487 0.0 10 1.7602410826186754E-4 0.0 0.0 6.999158604762508 0.0 11 1.7602410826186754E-4 0.0 0.0 8.15299663441905 0.0 12 1.7602410826186754E-4 0.0 0.0 9.324173038739385 0.0 13 1.7602410826186754E-4 0.0 0.0 9.722603607790123 0.0 14 1.7602410826186754E-4 0.0 0.0 9.891850787883909 0.0 15 1.7602410826186754E-4 0.0 0.0 10.211950628758114 0.0 16 1.7602410826186754E-4 0.0 0.0 10.800927295002323 0.0 17 2.640361623928013E-4 0.0 0.0 11.52720276569079 0.0 18 2.640361623928013E-4 0.0 0.0 12.372118485347753 0.0 19 2.640361623928013E-4 0.0 0.0 12.924482137073493 0.0 20 2.640361623928013E-4 0.0 0.0 13.412068916958866 0.0 21 2.640361623928013E-4 0.0 0.0 13.967248954416798 0.0 22 2.640361623928013E-4 0.0 0.0 14.579812851168096 0.0 23 2.640361623928013E-4 0.0 0.0 15.143970118147381 0.0 24 2.640361623928013E-4 0.0 0.0 15.607441595200878 0.0 25 3.5204821652373507E-4 0.0 0.0 15.998919211975272 0.0 26 4.4006027065466885E-4 0.0 0.0 16.380187430470478 0.0 27 4.4006027065466885E-4 0.0 0.0 16.759959444045457 0.0 28 4.4006027065466885E-4 0.0 0.0 17.12776181825863 0.0 29 4.4006027065466885E-4 0.0 0.0 17.508149916212524 0.0 30 4.4006027065466885E-4 0.0 0.0 17.96105994677031 0.0 31 4.4006027065466885E-4 0.0 0.0 18.365915395772603 0.0 32 4.4006027065466885E-4 0.0 0.0 18.755984819680904 0.0 33 4.4006027065466885E-4 0.0 0.0 19.136020869418275 0.0 34 4.4006027065466885E-4 0.0 0.0 19.517025051751087 0.0 35 4.4006027065466885E-4 0.0 0.0 19.897589173813245 0.0 36 4.4006027065466885E-4 0.0 0.0 20.298308056271388 0.0 37 5.280723247856026E-4 0.0 0.0 20.679136214495937 0.0 38 5.280723247856026E-4 0.0 0.0 21.051339191415657 0.0 39 6.160843789165364E-4 0.0 0.0 21.431023192936504 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTCG 30 2.1654796E-6 45.000004 1 TAGCCGT 30 2.1654796E-6 45.000004 44 CGCCTCG 30 2.1654796E-6 45.000004 24 ACTAACG 30 2.1654796E-6 45.000004 1 ACGTTGA 20 7.0332526E-4 45.0 10 TATATCG 20 7.0332526E-4 45.0 13 ATATACG 20 7.0332526E-4 45.0 1 CGTTTTT 31010 0.0 44.56466 1 TACGAAT 70 0.0 41.785713 12 GCTACGA 70 0.0 41.785713 10 CGAATAT 65 0.0 41.538464 14 CGGTCTA 55 6.184564E-11 40.90909 31 GTTTTTT 34505 0.0 40.226776 2 TCGTGTA 45 1.9283107E-8 40.0 17 CTAACGG 45 1.9283107E-8 40.0 2 CGGGCTA 45 1.9283107E-8 40.0 6 CGTAAGG 135 0.0 40.0 2 TATCACG 40 3.4581717E-7 39.375 1 TAGAACG 40 3.4581717E-7 39.375 1 TAGTGCG 75 0.0 39.0 1 >>END_MODULE