##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934866.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1471562
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67789192708156	34.0	31.0	34.0	31.0	34.0
2	32.82620372094414	34.0	31.0	34.0	31.0	34.0
3	32.87682000486558	34.0	31.0	34.0	31.0	34.0
4	36.259526272083676	37.0	37.0	37.0	35.0	37.0
5	36.1582563289892	37.0	37.0	37.0	35.0	37.0
6	36.15439580527358	37.0	37.0	37.0	35.0	37.0
7	36.25989594729953	37.0	37.0	37.0	35.0	37.0
8	36.26835294741234	37.0	37.0	37.0	35.0	37.0
9	38.04547276975078	39.0	39.0	39.0	35.0	39.0
10	37.6819930115075	39.0	38.0	39.0	35.0	39.0
11	37.604616726988056	39.0	37.0	39.0	35.0	39.0
12	37.56080817525867	39.0	37.0	39.0	35.0	39.0
13	37.53291536476207	39.0	37.0	39.0	35.0	39.0
14	38.8621328900855	40.0	38.0	41.0	35.0	41.0
15	38.92443199810813	40.0	38.0	41.0	35.0	41.0
16	38.98594486674703	41.0	38.0	41.0	35.0	41.0
17	39.00863504222044	41.0	38.0	41.0	35.0	41.0
18	38.493739305581414	39.0	38.0	41.0	35.0	41.0
19	37.97860980373236	38.0	37.0	41.0	35.0	41.0
20	37.38792860919214	38.0	35.0	41.0	35.0	41.0
21	37.334187074686625	38.0	35.0	41.0	34.0	41.0
22	37.157336218249725	38.0	35.0	41.0	34.0	41.0
23	37.0423006302147	38.0	35.0	40.0	34.0	41.0
24	36.852091179304715	38.0	35.0	40.0	33.0	41.0
25	36.70103400332436	37.0	35.0	40.0	33.0	41.0
26	36.812776491918115	38.0	35.0	40.0	33.0	41.0
27	36.85388790958179	38.0	35.0	40.0	34.0	41.0
28	36.77591565968678	38.0	35.0	40.0	33.0	41.0
29	36.62340628529413	38.0	35.0	40.0	33.0	41.0
30	36.34534868391546	38.0	35.0	40.0	33.0	41.0
31	35.81372990060901	38.0	35.0	40.0	31.0	41.0
32	35.05454000578977	38.0	35.0	40.0	23.0	41.0
33	34.094415321950414	38.0	34.0	41.0	16.0	41.0
34	33.2573075412385	38.0	33.0	41.0	12.0	41.0
35	32.71166964083063	38.0	33.0	41.0	10.0	41.0
36	32.456453754581865	38.0	32.0	41.0	8.0	41.0
37	32.1957178834463	37.0	31.0	40.0	8.0	41.0
38	32.04157079348339	37.0	30.0	40.0	7.0	41.0
39	31.943318052518343	37.0	30.0	40.0	7.0	41.0
40	31.795940639945854	37.0	29.0	40.0	7.0	41.0
41	31.666182600529233	37.0	29.0	40.0	7.0	41.0
42	31.53495877169973	37.0	28.0	40.0	7.0	41.0
43	31.42702448146935	37.0	27.0	40.0	7.0	41.0
44	31.305299402947345	36.0	26.0	40.0	7.0	41.0
45	31.268748445529308	36.0	25.0	40.0	7.0	41.0
46	31.231464253629817	36.0	25.0	40.0	7.0	41.0
47	31.21537182938945	36.0	25.0	40.0	7.0	41.0
48	31.16137478407298	36.0	25.0	40.0	7.0	41.0
49	31.02397248637842	36.0	24.0	40.0	7.0	41.0
50	30.913451828737085	35.0	24.0	40.0	7.0	41.0
51	30.349425304540347	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	14.0
11	14.0
12	22.0
13	30.0
14	30.0
15	48.0
16	102.0
17	229.0
18	429.0
19	914.0
20	1478.0
21	2567.0
22	4236.0
23	7451.0
24	15087.0
25	32561.0
26	59280.0
27	76599.0
28	71314.0
29	55931.0
30	42771.0
31	36669.0
32	34662.0
33	40920.0
34	71307.0
35	101237.0
36	96758.0
37	129259.0
38	240890.0
39	348416.0
40	325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.84461409033394	15.731311354873256	17.490122740326267	36.93395181446653
2	51.367118748649396	18.43992981607299	17.394034366204075	12.798917069073543
3	23.592278137108732	18.033558898639676	45.19999836907993	13.17416459517166
4	21.468684296006558	19.4511002594522	45.571032684997306	13.509182759543942
5	18.85064985369288	22.38716411540934	44.24475489309999	14.517431137797795
6	19.3077831583039	26.057006092845565	43.441526758641494	11.193683990209042
7	58.094731992264	5.616616900952865	32.61350863911952	3.675142467663612
8	58.703676773387734	5.093363378505289	32.22405851741211	3.9789013306948675
9	53.80826631837462	6.80888742710127	34.385911025155586	4.996935229368522
10	29.276170490947713	19.462924429959457	39.65086078602193	11.610044293070901
11	23.26935596325537	19.478214305615392	43.22400279430972	14.028426936819516
12	20.743944189915204	18.214251251391378	46.20335398712388	14.838450571569531
13	17.95880839543288	20.617411974486973	47.50211000284052	13.921669627239627
14	14.684804309978105	22.192812807071668	47.527932903948326	15.594449979001904
15	14.726052996747674	19.935891250249735	50.31816532364929	15.0198904293533
16	16.368729282218485	19.921484789631698	48.40407675653489	15.305709171614923
17	16.580409116299553	19.696145999964664	46.0178368291652	17.705608054570586
18	16.702931986555782	19.848229296489038	47.49259630243238	15.9562424145228
19	17.989320191741836	20.2325827929778	46.19445188174199	15.583645133538376
20	18.15098514367726	20.59722933862114	46.411704026062104	14.840081491639495
21	17.60224849513646	20.959497459162442	46.54231354166525	14.895940504035849
22	16.708096566777343	19.727473256308603	45.61391229183684	17.950517885077215
23	16.36193378192696	20.068811235952	46.634052795600866	16.935202186520172
24	16.116820086411582	19.37220450106757	47.63849569369146	16.872479718829382
25	15.241627603865822	21.418193728840514	46.288569560779635	17.051609106514032
26	14.910754694671377	22.626093905659427	46.30990743169503	16.153243967974166
27	16.30933660967054	21.426891969213667	46.9461701239907	15.317601297125098
28	14.308129728818766	21.479353231464255	48.35263481932803	15.859882220388947
29	15.148801069883566	21.07685574919711	46.94549057396154	16.828852606957778
30	16.03507021790451	21.79085896482785	45.982772047660916	16.191298769606718
31	17.005263794525817	22.99067249629985	43.713754500320064	16.290309208854264
32	17.185276597248368	24.040985021358257	41.85124378041836	16.922494600975018
33	17.52369251176641	24.05878923212206	40.75642072845045	17.661097527661084
34	16.113354381262905	25.66986644123727	39.29898978092666	18.917789396573166
35	17.54611766272845	25.789331336362313	37.45992353703072	19.204627463878516
36	19.490378251137226	27.09236851726261	36.11624926438709	17.30100396721307
37	17.92517066898982	28.00500420641468	36.93007837930036	17.139746745295135
38	18.43816298599719	28.182094944011872	34.70625090889816	18.67349116109277
39	20.28402473018466	27.049217090411414	34.46426314351689	18.202495035887036
40	19.53067556786598	27.351684808387276	34.729627429901015	18.388012193845725
41	17.972535306021765	27.236365168440063	34.00475141380384	20.786348111734334
42	18.7167784979498	26.91262753455172	34.18184215140103	20.188751816097454
43	19.64810181290357	25.80448530201242	35.403537193811744	19.143875691272267
44	18.168585489432317	26.722353526388964	34.94558842916574	20.163472555012973
45	17.975117596132545	26.915617554679994	34.55226487229216	20.5569999768953
46	18.848950978619996	26.51318802741577	35.2835966136663	19.35426438029794
47	17.75093404151507	25.274640144282063	37.67309838117592	19.301327433026945
48	17.74950698645385	24.592779645030248	37.38571667384725	20.27199669466866
49	18.763395629949674	23.863418598740658	37.99676806006135	19.37641771124832
50	17.64519605697891	24.28630258188238	37.34983643230798	20.718664928830727
51	17.60224849513646	24.17499228710717	35.65198068446997	22.570778533286397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	557.0
1	1042.5
2	1528.0
3	58670.0
4	115812.0
5	81491.0
6	47170.0
7	43850.5
8	40531.0
9	39105.5
10	37680.0
11	36182.0
12	34684.0
13	32945.0
14	31206.0
15	28377.5
16	25549.0
17	23310.0
18	21071.0
19	19063.5
20	17056.0
21	16059.5
22	15063.0
23	13722.0
24	12381.0
25	13688.0
26	15006.0
27	15017.0
28	16974.5
29	18932.0
30	20500.0
31	22068.0
32	25142.5
33	28217.0
34	29304.5
35	30392.0
36	32410.0
37	34428.0
38	39365.0
39	44302.0
40	49337.0
41	54372.0
42	63234.0
43	72096.0
44	85856.0
45	99616.0
46	108864.0
47	118112.0
48	112703.0
49	107294.0
50	92183.5
51	77073.0
52	69341.0
53	61609.0
54	55698.5
55	49788.0
56	46759.5
57	43731.0
58	41864.5
59	39998.0
60	37924.0
61	35850.0
62	32099.5
63	28349.0
64	25535.5
65	22722.0
66	19087.0
67	15452.0
68	13899.0
69	12346.0
70	10546.5
71	8747.0
72	7702.0
73	6657.0
74	5259.0
75	3167.5
76	2474.0
77	1792.5
78	1111.0
79	1027.0
80	943.0
81	687.0
82	431.0
83	334.0
84	237.0
85	130.0
86	23.0
87	20.5
88	18.0
89	11.0
90	4.0
91	4.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1471562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.21054166815163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54389940935195	24.20187103626538
2	9.66860061936545	6.035245250068441
3	3.690692869589095	3.4556557077198167
4	1.9098853807497107	2.3843422902912996
5	1.1234660724076064	1.753199233281613
6	0.7307448761447134	1.3684166043421728
7	0.5180693317797689	1.1318457126552766
8	0.37288679318558865	0.9310399036977804
9	0.28968346738785766	0.8137060136535038
>10	2.981141703735047	22.86811369845457
>50	1.0427139804244654	21.95469141987425
>100	0.11908904577759695	6.322577118482204
>500	0.005787504942175496	1.2610834198824608
>1k	0.0031163488150175753	1.4809671254756729
>5k	0.0	0.0
>10k+	2.225963439298268E-4	4.037245465855529
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58112	3.949001129412148	No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	3377	0.2294840448448655	No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC	2862	0.19448721834350166	No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT	2136	0.1451518862270159	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.795500291526962E-5	0.0	0.0	0.1574517417546797	0.0
2	6.795500291526962E-5	0.0	0.0	0.765173332825936	0.0
3	6.795500291526962E-5	0.0	0.0	1.1050163024051993	0.0
4	1.3591000583053925E-4	0.0	0.0	1.4681678379844003	0.0
5	1.3591000583053925E-4	0.0	0.0	2.371357781731249	0.0
6	1.3591000583053925E-4	0.0	0.0	2.8882235339047897	0.0
7	1.3591000583053925E-4	0.0	0.0	3.4015556259267363	0.0
8	1.3591000583053925E-4	0.0	0.0	4.26934101315473	0.0
9	1.3591000583053925E-4	0.0	0.0	4.6051746375619915	0.0
10	1.3591000583053925E-4	0.0	0.0	5.321488323291849	0.0
11	1.3591000583053925E-4	0.0	0.0	6.364257843026661	0.0
12	1.3591000583053925E-4	0.0	0.0	7.239382370569504	0.0
13	1.3591000583053925E-4	0.0	0.0	7.559314524294593	0.0
14	1.3591000583053925E-4	0.0	0.0	7.684419684661605	0.0
15	1.3591000583053925E-4	0.0	0.0	7.931775895273186	0.0
16	1.3591000583053925E-4	0.0	0.0	8.47704683866531	0.0
17	1.3591000583053925E-4	0.0	0.0	9.115823866068844	0.0
18	1.3591000583053925E-4	0.0	0.0	9.894995929495325	0.0
19	1.3591000583053925E-4	0.0	0.0	10.313123062432979	0.0
20	1.3591000583053925E-4	0.0	0.0	10.750617371201486	0.0
21	1.3591000583053925E-4	0.0	0.0	11.253552347777395	0.0
22	2.038650087458089E-4	0.0	0.0	11.778504745297854	0.0
23	2.038650087458089E-4	0.0	0.0	12.279876756806713	0.0
24	2.718200116610785E-4	0.0	0.0	12.69365476955779	0.0
25	2.718200116610785E-4	0.0	0.0	13.066387960548044	0.0
26	2.718200116610785E-4	0.0	0.0	13.411667330360528	0.0
27	2.718200116610785E-4	0.0	0.0	13.762790830423727	0.0
28	2.718200116610785E-4	0.0	0.0	14.12662191603208	0.0
29	2.718200116610785E-4	0.0	0.0	14.501801487127285	0.0
30	2.718200116610785E-4	0.0	0.0	14.935490315732535	0.0
31	2.718200116610785E-4	0.0	0.0	15.341181683136695	0.0
32	2.718200116610785E-4	0.0	0.0	15.733621824972376	0.0
33	2.718200116610785E-4	0.0	0.0	16.114305751303718	0.0
34	2.718200116610785E-4	0.0	0.0	16.48160254206075	0.0
35	2.718200116610785E-4	0.0	0.0	16.856714158153036	0.0
36	2.718200116610785E-4	0.0	0.0	17.238961049551428	0.0
37	2.718200116610785E-4	0.0	0.0	17.59749164493239	0.0
38	2.718200116610785E-4	0.0	0.0	17.958876350435794	0.0
39	2.718200116610785E-4	0.0	0.0	18.323522896079133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCG	20	7.033933E-4	45.0	31
GGTACGT	20	7.033933E-4	45.0	9
CGCATCG	25	3.8913404E-5	45.0	21
CGCGAAC	20	7.033933E-4	45.0	40
TAACGAC	20	7.033933E-4	45.0	31
GTAATCG	25	3.8913404E-5	45.0	1
CGCCTAT	25	3.8913404E-5	45.0	42
CCGTATC	20	7.033933E-4	45.0	6
GTTAACG	20	7.033933E-4	45.0	29
CTACGCC	25	3.8913404E-5	45.0	26
AACGATA	25	3.8913404E-5	45.0	40
TATAGCG	20	7.033933E-4	45.0	1
ATACGTT	25	3.8913404E-5	45.0	2
AAATGCG	50	2.1827873E-11	45.0	1
ACGCGAG	30	2.165918E-6	44.999996	1
CGTTTTT	40455	0.0	44.55506	1
TCAACGG	100	0.0	42.75	2
GTGTACG	65	0.0	41.538464	1
GACTCGT	65	0.0	41.538464	29
TAGGGCG	470	0.0	41.17021	5
>>END_MODULE