##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934865.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	972967
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16394389532225	33.0	31.0	34.0	30.0	34.0
2	32.34730468762044	34.0	31.0	34.0	30.0	34.0
3	32.340773119746096	34.0	31.0	34.0	30.0	34.0
4	35.888296314263485	37.0	35.0	37.0	35.0	37.0
5	35.79786364799628	37.0	35.0	37.0	35.0	37.0
6	35.719558833958395	37.0	35.0	37.0	33.0	37.0
7	36.07494087672038	37.0	35.0	37.0	35.0	37.0
8	35.939546767773216	37.0	35.0	37.0	35.0	37.0
9	37.682387994659635	39.0	38.0	39.0	35.0	39.0
10	37.25592337664073	39.0	37.0	39.0	34.0	39.0
11	37.173440620288254	39.0	37.0	39.0	34.0	39.0
12	37.06808144572221	39.0	37.0	39.0	34.0	39.0
13	36.980600575353535	39.0	37.0	39.0	33.0	39.0
14	38.11050734505898	40.0	37.0	41.0	33.0	41.0
15	38.16194485527259	40.0	37.0	41.0	33.0	41.0
16	38.296251568655464	40.0	37.0	41.0	34.0	41.0
17	38.27309970430652	40.0	37.0	41.0	34.0	41.0
18	38.24647906866317	40.0	37.0	41.0	34.0	41.0
19	38.18599191956151	40.0	37.0	41.0	34.0	41.0
20	38.13959055137533	40.0	36.0	41.0	34.0	41.0
21	38.05500186542812	40.0	36.0	41.0	34.0	41.0
22	38.00702490423622	40.0	36.0	41.0	34.0	41.0
23	37.84282817402851	40.0	36.0	41.0	33.0	41.0
24	37.5905894033405	39.0	35.0	41.0	33.0	41.0
25	37.55285328279376	39.0	35.0	41.0	33.0	41.0
26	37.715906089312384	39.0	36.0	41.0	33.0	41.0
27	37.74839845544607	39.0	36.0	41.0	33.0	41.0
28	37.728423471710755	40.0	36.0	41.0	33.0	41.0
29	37.71882910725646	40.0	36.0	41.0	33.0	41.0
30	37.55172580365007	39.0	36.0	41.0	33.0	41.0
31	37.46767053764413	39.0	36.0	41.0	33.0	41.0
32	37.34193554354875	39.0	35.0	41.0	33.0	41.0
33	37.194642778223724	39.0	35.0	41.0	32.0	41.0
34	37.169795070130846	39.0	35.0	41.0	32.0	41.0
35	37.05621465065105	39.0	35.0	41.0	32.0	41.0
36	36.927394248725804	39.0	35.0	41.0	31.0	41.0
37	36.85757070897574	39.0	35.0	41.0	31.0	41.0
38	36.80086786088326	39.0	35.0	41.0	31.0	41.0
39	36.86545792406115	39.0	35.0	41.0	31.0	41.0
40	36.83720516728728	39.0	35.0	41.0	31.0	41.0
41	36.76859235719197	39.0	35.0	41.0	31.0	41.0
42	36.642231442587466	39.0	35.0	41.0	31.0	41.0
43	36.574083190899586	39.0	35.0	41.0	31.0	41.0
44	36.51453029753321	39.0	35.0	41.0	31.0	41.0
45	36.48642040274747	39.0	35.0	41.0	31.0	41.0
46	36.43882886058828	39.0	35.0	41.0	31.0	41.0
47	36.31197563740599	39.0	35.0	41.0	30.0	41.0
48	36.260953351963636	39.0	35.0	41.0	30.0	41.0
49	36.2516601282469	39.0	35.0	41.0	30.0	41.0
50	36.156496571826175	39.0	35.0	40.0	30.0	41.0
51	35.18643797785536	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	6.0
10	11.0
11	10.0
12	10.0
13	14.0
14	15.0
15	31.0
16	46.0
17	70.0
18	204.0
19	368.0
20	669.0
21	1139.0
22	1677.0
23	2628.0
24	4137.0
25	6442.0
26	8638.0
27	9714.0
28	10446.0
29	11433.0
30	13651.0
31	17625.0
32	23233.0
33	32061.0
34	57929.0
35	82021.0
36	70155.0
37	111163.0
38	209063.0
39	298128.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.562937900257666	22.276295085033716	24.315315935689494	12.845451079019124
2	34.50867295602009	24.43895836138327	24.31243814024525	16.739930542351384
3	30.830850378275933	23.110855763864553	28.062719496139128	17.995574361720386
4	27.206575351476463	25.807658430347587	28.80467682871053	18.18108938946542
5	23.739345733205752	29.249399003254993	27.39630429397914	19.61495096956012
6	24.156934407847334	33.47605828358002	27.343270635078063	15.023736673494579
7	79.26856717648182	5.39627757159287	10.126345497843195	5.208809754082101
8	80.3355098374354	3.748431344536865	9.972383441576127	5.943675376451616
9	75.67378955298587	5.316315969606369	12.678436164844234	6.33145831256353
10	38.34189648775344	20.24816874570258	23.99290006752541	17.41703469901857
11	29.4142555708467	25.968095526364205	23.918385721201233	20.699263181587867
12	25.321619335496475	23.06748327538344	31.62512192088735	19.985775468232735
13	22.181019500147485	26.924859733166695	32.500793963207386	18.393326803478434
14	20.272116114935038	29.975734017700496	29.971828438169023	19.780321429195443
15	18.461982780505405	27.18427243678357	35.78394745145519	18.569797331255838
16	20.677063045303694	24.651401332213734	33.18478427325901	21.48675134922356
17	20.60583760805865	25.30733313668398	28.833660339970418	25.25316891528695
18	21.0111956520622	26.257108411693302	32.68065617847265	20.051039757771846
19	21.59364089429549	28.258717921573904	29.49740330350361	20.65023788062699
20	22.742395168592562	27.173480703867654	30.163612948846158	19.920511178693626
21	21.438959389167362	27.231961618431043	31.529024108731335	19.80005488367026
22	19.64342058877639	26.05925997490151	28.751848726626903	25.545470709695188
23	18.654075626408705	28.169095149167443	29.434708474182575	23.742120750241273
24	21.202980162739333	25.422650511271193	31.27310587101104	22.101263454978433
25	18.717901018225692	27.770520480139616	29.594117786111962	23.917460715522726
26	19.885977633362696	28.84866598764398	29.908516938395653	21.35683944059768
27	21.672574712194763	27.91441025235183	30.862197792936453	19.55081724251696
28	19.806118809784916	26.21003590049817	32.136752839510486	21.847092450206432
29	21.40134249157474	24.635162343635496	32.12483054409862	21.838664620691144
30	21.196505122989784	25.503023226892584	32.57705554247986	20.72341610763777
31	23.274376212142858	26.369650769245002	28.296437597575252	22.05953542103689
32	23.010235701724724	25.48616756786201	30.341830709571855	21.161766020841405
33	21.33576986680946	24.603301036931366	32.313840037740235	21.74708905851894
34	20.672026903276265	23.691348216332106	31.54331030754383	24.09331457284779
35	21.88912881937414	23.588569807609097	31.321308944702132	23.200992428314628
36	23.29431522343512	23.32463485400841	33.16135079607017	20.219699126486304
37	21.481817985604856	24.738043530767232	32.97223852401983	20.807899959608086
38	20.680660289609	26.231208252695108	31.2881115186846	21.800019939011293
39	20.716221619027163	24.294451918718725	32.51384682111521	22.475479641138907
40	22.432312709475244	22.854423634100645	31.37362315474214	23.33964050168197
41	21.366808946243808	23.60676158595307	29.10047308901535	25.925956378787767
42	23.1910229226685	22.998724519947746	30.997145843589763	22.813106713793992
43	23.280851251892408	23.185164553371283	32.152375157636385	21.38160903709992
44	21.12527968574474	23.93801639726733	30.94555108241081	23.99115283457712
45	21.424364855128694	23.356907274347435	29.60336784289704	25.61536002762684
46	23.3728379276995	23.491546989774577	30.592815583673445	22.542799498852478
47	20.174784961874348	23.57253637584831	33.55046985149548	22.702208810781865
48	21.664557996314365	21.588090860224447	32.41055452034858	24.3367966231126
49	21.33268651454777	20.45526723927944	34.84126388664775	23.37078235952504
50	20.658768488551	21.00996231115752	32.723823110136316	25.607446090155168
51	20.054637002077154	21.624474416912392	29.35587743469203	28.965011146318425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	412.0
1	566.5
2	721.0
3	3303.0
4	5885.0
5	4248.0
6	2611.0
7	2561.0
8	2511.0
9	2625.0
10	2739.0
11	2849.5
12	2960.0
13	2911.5
14	2863.0
15	2836.5
16	2810.0
17	2739.5
18	2669.0
19	2965.5
20	3262.0
21	3033.0
22	2804.0
23	3335.5
24	3867.0
25	4373.0
26	5143.0
27	5407.0
28	7476.5
29	9546.0
30	12854.0
31	16162.0
32	15629.0
33	15096.0
34	18970.0
35	22844.0
36	25070.0
37	27296.0
38	31066.5
39	34837.0
40	40305.0
41	45773.0
42	52877.0
43	59981.0
44	69071.5
45	78162.0
46	90608.5
47	103055.0
48	116670.5
49	130286.0
50	126065.5
51	121845.0
52	102241.0
53	82637.0
54	70201.5
55	57766.0
56	48692.5
57	39619.0
58	34281.5
59	28944.0
60	25137.5
61	21331.0
62	17591.0
63	13851.0
64	10942.0
65	8033.0
66	5913.5
67	3794.0
68	2902.0
69	2010.0
70	1854.0
71	1698.0
72	1296.0
73	894.0
74	693.5
75	470.0
76	447.0
77	250.0
78	53.0
79	57.5
80	62.0
81	46.0
82	30.0
83	21.0
84	12.0
85	8.5
86	5.0
87	4.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	972967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.818225853289757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.45876546340239	17.416656425182257
2	12.818973089395458	6.61926284858511
3	5.903683179557676	4.572678770882636
4	3.245229914647669	3.3514431552890307
5	1.9397099403109401	2.5039934664409524
6	1.299412547678774	2.012911597954154
7	0.8809479847979614	1.5921159825559965
8	0.6802991133331839	1.4051292924663128
9	0.5013300140599929	1.164910637703044
>10	3.728038535384236	21.545706741232046
>50	1.2046024074136488	22.23974645894974
>100	0.32976944552708437	11.621227996934525
>500	0.005221684277529953	0.9179838859637015
>1k	0.0032133441707876634	1.7840122899043784
>5k	8.033360426969159E-4	1.2522204499561338
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	6190	0.6361983499954263	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	5885	0.6048509353349085	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	3981	0.40916084512629924	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3151	0.3238547658862017	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	2253	0.2315597548529395	No Hit
ACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	2129	0.2188152321712864	No Hit
GGATCAGGGGCTTGAGTTTTCCATACAAAAGATTTTTAAATTGTAATAACT	2013	0.2068929367594173	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1616	0.16608990849638272	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1047	0.10760899393299055	No Hit
GGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	1013	0.10411452803640822	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	998	0.10257285190556308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.111136348920364E-4	0.0	0.0	0.4334165495849294	0.0
2	4.111136348920364E-4	0.0	0.0	2.2333748215509877	0.0
3	4.111136348920364E-4	0.0	0.0	3.079241125341353	0.0
4	4.111136348920364E-4	0.0	0.0	3.976599411901945	0.0
5	4.111136348920364E-4	0.0	0.0	6.187465761942594	0.0
6	4.111136348920364E-4	0.0	0.0	7.34896455892132	0.0
7	4.111136348920364E-4	0.0	0.0	8.429268413008868	0.0
8	4.111136348920364E-4	0.0	0.0	10.034564378853547	0.0
9	4.111136348920364E-4	0.0	0.0	10.579495501902942	0.0
10	4.111136348920364E-4	0.0	0.0	12.053954553443232	0.0
11	4.111136348920364E-4	0.0	0.0	14.345296397513996	0.0
12	4.111136348920364E-4	0.0	0.0	15.896839255596541	0.0
13	4.111136348920364E-4	0.0	0.0	16.481751179639186	0.0
14	4.111136348920364E-4	0.0	0.0	16.712385928813617	0.0
15	4.111136348920364E-4	0.0	0.0	17.129974603455203	0.0
16	4.111136348920364E-4	0.0	0.0	18.25663151987683	0.0
17	4.111136348920364E-4	0.0	0.0	19.59583418553764	0.0
18	4.111136348920364E-4	0.0	0.0	21.068134890494743	0.0
19	5.138920436150456E-4	0.0	0.0	21.907320597718115	0.0
20	5.138920436150456E-4	0.0	0.0	22.662125231379893	0.0
21	5.138920436150456E-4	0.0	0.0	23.682406494773204	0.0
22	5.138920436150456E-4	0.0	0.0	24.77802433176048	0.0
23	5.138920436150456E-4	0.0	0.0	25.90725070840018	0.0
24	5.138920436150456E-4	0.0	0.0	26.77685882460556	0.0
25	5.138920436150456E-4	0.0	0.0	27.503707731094682	0.0
26	5.138920436150456E-4	0.0	0.0	28.218737120580656	0.0
27	5.138920436150456E-4	0.0	0.0	28.863877192134986	1.027784087230091E-4
28	5.138920436150456E-4	0.0	0.0	29.556500888519345	1.027784087230091E-4
29	5.138920436150456E-4	0.0	0.0	30.293010965428426	1.027784087230091E-4
30	5.138920436150456E-4	0.0	0.0	31.05614065019677	1.027784087230091E-4
31	5.138920436150456E-4	0.0	0.0	31.80755359637069	1.027784087230091E-4
32	5.138920436150456E-4	0.0	0.0	32.51569683247222	1.027784087230091E-4
33	5.138920436150456E-4	0.0	0.0	33.192081540278345	1.027784087230091E-4
34	5.138920436150456E-4	0.0	0.0	33.83023268003951	1.027784087230091E-4
35	5.138920436150456E-4	0.0	0.0	34.49099506971973	1.027784087230091E-4
36	5.138920436150456E-4	0.0	0.0	35.14980466963422	1.027784087230091E-4
37	5.138920436150456E-4	0.0	0.0	35.77778074693181	1.027784087230091E-4
38	5.138920436150456E-4	0.0	0.0	36.416137443510415	1.027784087230091E-4
39	5.138920436150456E-4	0.0	0.0	37.03373290152698	1.027784087230091E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	20	7.0327515E-4	45.000004	2
AGCGTCA	20	7.0327515E-4	45.000004	35
CGTGGCG	30	2.1651558E-6	45.000004	27
CAGCGAC	20	7.0327515E-4	45.000004	36
GATACGA	30	2.1651558E-6	45.000004	13
ACCGGTG	20	7.0327515E-4	45.000004	41
ACGCCCG	20	7.0327515E-4	45.000004	13
GATCGAC	20	7.0327515E-4	45.000004	20
CGACGTC	20	7.0327515E-4	45.000004	39
ACGTTCA	20	7.0327515E-4	45.000004	42
GCGCGAA	30	2.1651558E-6	45.000004	35
CGGTTAG	20	7.0327515E-4	45.000004	14
CACGCTT	20	7.0327515E-4	45.000004	24
ATTCGCG	30	2.1651558E-6	45.000004	1
GTCTACG	35	1.2116652E-7	45.000004	1
ATATGCG	30	2.1651558E-6	45.000004	1
CGCGAGA	20	7.0327515E-4	45.000004	26
AAGCGAC	20	7.0327515E-4	45.000004	29
TAACGCG	20	7.0327515E-4	45.000004	1
TCGGCTA	20	7.0327515E-4	45.000004	30
>>END_MODULE