##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934863.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2188378
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26742454914096	33.0	31.0	34.0	30.0	34.0
2	32.44320222557529	34.0	31.0	34.0	30.0	34.0
3	32.45628177581752	34.0	31.0	34.0	30.0	34.0
4	35.99229337893179	37.0	35.0	37.0	35.0	37.0
5	35.87372154170806	37.0	35.0	37.0	35.0	37.0
6	35.794699544594216	37.0	35.0	37.0	35.0	37.0
7	36.05171135882375	37.0	35.0	37.0	35.0	37.0
8	35.76808439858196	37.0	35.0	37.0	35.0	37.0
9	37.48773657932953	39.0	37.0	39.0	35.0	39.0
10	37.29781006754774	39.0	37.0	39.0	34.0	39.0
11	37.17642473101082	39.0	37.0	39.0	34.0	39.0
12	37.10953043761178	39.0	37.0	39.0	34.0	39.0
13	37.06809381194657	39.0	37.0	39.0	33.0	39.0
14	38.26414129551659	40.0	38.0	41.0	33.0	41.0
15	38.28972051446323	40.0	38.0	41.0	34.0	41.0
16	38.422042718396916	40.0	38.0	41.0	34.0	41.0
17	38.31457682356522	40.0	37.0	41.0	34.0	41.0
18	38.3065754636539	40.0	37.0	41.0	34.0	41.0
19	38.31148823466513	40.0	37.0	41.0	34.0	41.0
20	38.154909709382935	40.0	37.0	41.0	34.0	41.0
21	38.156388430152376	40.0	37.0	41.0	34.0	41.0
22	38.08679579122071	40.0	37.0	41.0	34.0	41.0
23	37.864196222042075	40.0	36.0	41.0	33.0	41.0
24	37.64078143721057	40.0	36.0	41.0	33.0	41.0
25	37.625004912314054	40.0	36.0	41.0	33.0	41.0
26	37.72830836354597	40.0	36.0	41.0	33.0	41.0
27	37.763589288505	40.0	36.0	41.0	33.0	41.0
28	37.67611262770874	40.0	36.0	41.0	33.0	41.0
29	37.680114678542736	40.0	36.0	41.0	33.0	41.0
30	37.57447387974107	40.0	36.0	41.0	33.0	41.0
31	37.51510707930714	40.0	36.0	41.0	33.0	41.0
32	37.40079136236975	40.0	36.0	41.0	32.0	41.0
33	37.2713653674091	40.0	36.0	41.0	32.0	41.0
34	37.19305851182931	40.0	36.0	41.0	32.0	41.0
35	37.116652150588244	40.0	35.0	41.0	32.0	41.0
36	37.03455847207384	40.0	35.0	41.0	31.0	41.0
37	37.02871213291305	40.0	35.0	41.0	31.0	41.0
38	36.88825924954464	40.0	35.0	41.0	31.0	41.0
39	36.904350162540474	39.0	35.0	41.0	31.0	41.0
40	36.80512370349181	39.0	35.0	41.0	31.0	41.0
41	36.71296183748877	39.0	35.0	41.0	31.0	41.0
42	36.66135786413499	39.0	35.0	41.0	31.0	41.0
43	36.58196984250436	39.0	35.0	41.0	31.0	41.0
44	36.62298926419476	39.0	35.0	41.0	31.0	41.0
45	36.57225305683022	39.0	35.0	41.0	31.0	41.0
46	36.502162789061124	39.0	35.0	41.0	31.0	41.0
47	36.34100187444765	39.0	35.0	41.0	30.0	41.0
48	36.30260585694062	39.0	35.0	41.0	30.0	41.0
49	36.27238027434017	39.0	35.0	41.0	30.0	41.0
50	36.13292858911943	39.0	35.0	40.0	30.0	41.0
51	35.28921009076129	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	37.0
10	38.0
11	41.0
12	35.0
13	33.0
14	62.0
15	62.0
16	161.0
17	271.0
18	602.0
19	1228.0
20	2389.0
21	4251.0
22	6694.0
23	9412.0
24	11513.0
25	13885.0
26	16761.0
27	19132.0
28	21203.0
29	25060.0
30	30754.0
31	39616.0
32	51210.0
33	70539.0
34	125676.0
35	160197.0
36	157745.0
37	242821.0
38	439422.0
39	736981.0
40	538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52663113959289	20.79179191163501	23.54807990210101	11.133497046671097
2	30.300569645646224	28.499372594679713	24.216108917198035	16.983948842476025
3	30.341878779625826	27.855927997813907	25.530050110172926	16.27214311238735
4	27.532583493345296	24.328886508637904	29.98567889094114	18.15285110707565
5	28.86101030077985	28.622843037171826	23.26060671419654	19.255539947851787
6	25.715712733357766	36.86163907697848	22.71970381716504	14.702944372498719
7	80.08145759096463	4.823572527232498	10.958938538040503	4.136031343762366
8	80.8248392188187	7.916593933954736	7.026939587219393	4.231627260007183
9	75.05357849512288	6.580718687539355	8.220334878160902	10.14536793917687
10	41.721631272111125	29.71904305380515	15.546582902953695	13.012742771130034
11	29.764464822804836	24.643914351176992	26.528643588996054	19.062977237022125
12	27.921684462190715	21.202324278529577	28.962272514163455	21.913718745116245
13	26.140547930933323	21.9962456211861	31.47628060600134	20.386925841879236
14	20.050009641844323	28.222272386214815	27.981409061871393	23.746308910069466
15	18.41628822808491	26.517676562275806	33.50262157634559	21.5634136332937
16	21.359792503854454	26.329317878355567	29.463237155555394	22.847652462234585
17	21.92550829884051	26.472072009497445	29.375683725572088	22.226735966089954
18	21.242628101726485	26.460785111164526	29.00339886436438	23.293187922744607
19	23.07371030050567	27.75023327779753	26.610210850227883	22.565845571468916
20	26.21169651678092	26.918932652402827	28.579888849184194	18.289481981632058
21	24.3701956426175	28.473417298108462	27.946817231757947	19.209569827516088
22	22.5770410779125	24.06234206339124	29.76016940400607	23.600447454690183
23	22.844316658273844	26.965222644351204	28.97479320300241	21.215667494372546
24	23.08422036777924	24.815319839625513	28.844651152588813	23.255808640006435
25	21.655490961799103	29.194636392798685	26.160060099306428	22.989812546095784
26	21.52023096558273	26.92277111175492	28.22515123072888	23.331846691933478
27	23.38887523087876	27.08106186408381	27.08042212085846	22.449640784178968
28	20.019484750806306	28.1549622597193	29.336111037489864	22.489441951984528
29	23.85684740022062	24.755960807502177	27.71650967063277	23.670682121644433
30	22.0925726725456	27.097421012274847	27.638141125527678	23.17186518965188
31	22.66107592015639	27.295558628353966	25.44985372728112	24.593511724208522
32	24.732610179776984	27.6482856252439	25.31285728516737	22.30624690981174
33	23.208787512943378	27.23807312996201	25.865778215646472	23.68736114144814
34	23.31992005037521	26.15690707912436	28.330663166966584	22.19250970353385
35	23.72697952547503	24.765694043716397	26.688579395332983	24.81874703547559
36	22.478200749596276	28.976529648899778	26.972990954944713	21.57227864655923
37	21.737149614920273	27.083757924819203	28.02929841188314	23.149794048377384
38	21.473849581745018	28.606072625478777	25.964664239907364	23.955413552868837
39	22.489670431707868	25.29955976526907	28.274091587467982	23.936678215555084
40	23.476428660862066	25.66229417404123	28.631799442326688	22.229477722770017
41	20.72050623795341	26.014792691207827	29.724800742833274	23.53990032800549
42	22.821148814327323	25.50149014475561	28.541915519165332	23.135445521751727
43	22.60441294876845	25.781149326121906	28.00868954083801	23.60574818427164
44	21.664036103451963	25.80609931191046	27.69690611037033	24.83295847426724
45	21.784673397374675	24.483110321891374	27.236290988119972	26.495925292613983
46	23.604103130263603	26.63666880219048	26.559442655702075	23.19978541184384
47	20.614948605771033	25.802123764724378	31.064880016158085	22.518047613346507
48	21.805647835977148	24.869378142167395	28.822168747812306	24.502805274043148
49	21.35823884173575	24.143863628678407	30.545408517175737	23.952489012410105
50	20.92595520517936	24.386920358365877	29.499656823455545	25.187467612999214
51	20.270218399197944	24.000789625923858	27.84838816694374	27.880603807934463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	366.0
1	1123.0
2	1880.0
3	5454.5
4	9029.0
5	6434.0
6	3839.0
7	3708.0
8	3577.0
9	3528.5
10	3480.0
11	3441.5
12	3403.0
13	3543.5
14	3684.0
15	3518.5
16	3353.0
17	3600.0
18	3847.0
19	4298.5
20	4750.0
21	5150.0
22	5550.0
23	7495.0
24	9440.0
25	11653.5
26	17077.5
27	20288.0
28	25035.5
29	29783.0
30	34541.5
31	39300.0
32	46384.5
33	53469.0
34	60219.5
35	66970.0
36	70906.0
37	74842.0
38	80477.5
39	86113.0
40	93563.0
41	101013.0
42	106762.0
43	112511.0
44	125045.5
45	137580.0
46	183992.0
47	230404.0
48	211111.0
49	191818.0
50	189514.5
51	187211.0
52	165161.0
53	143111.0
54	130384.0
55	117657.0
56	111949.5
57	106242.0
58	100822.5
59	95403.0
60	96297.5
61	97192.0
62	88311.0
63	79430.0
64	65665.5
65	51901.0
66	43318.0
67	34735.0
68	28148.0
69	21561.0
70	18992.0
71	16423.0
72	13029.0
73	9635.0
74	7889.0
75	4759.0
76	3375.0
77	2814.5
78	2254.0
79	1613.0
80	972.0
81	772.5
82	573.0
83	384.0
84	195.0
85	171.0
86	147.0
87	95.5
88	44.0
89	26.5
90	9.0
91	7.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2188378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.70890245203552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.05521772945693	14.921844751195037
2	10.980682020350665	4.547957456325244
3	4.332679915033529	2.6917513714896866
4	2.1045510377369663	1.7433176858329986
5	1.2612499745011612	1.3059551344788414
6	0.8273652775516316	1.0280296095010817
7	0.5624560072845668	0.815349261189223
8	0.4251137975177546	0.7042912131047647
9	0.3254116031617811	0.6065025431974036
>10	4.521395715626159	24.058910566417367
>50	2.244803682047706	33.07955172617375
>100	0.35051852067776873	9.394643745032443
>500	0.0047276078978990095	0.6785649870641982
>1k	0.002926614412985101	1.497598287836705
>5k	2.2512418561423857E-4	0.33198634647091413
>10k+	6.753725568427156E-4	2.5937453146902554
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	20811	0.9509783044793907	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	17771	0.8120626326895993	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	17053	0.7792529444181947	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	7121	0.32540082197865267	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACATGTT	4283	0.19571573101173564	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4206	0.19219714327232315	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	3478	0.15893049555424155	No Hit
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	3295	0.15056813768005345	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTA	2777	0.1268976383421877	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	2673	0.12214526009674746	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	2595	0.1185809764126673	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	2320	0.10601459162905129	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8278377867077809E-4	0.0	0.0	0.1133716387205501	0.0
2	1.8278377867077809E-4	0.0	0.0	0.7445240264707468	0.0
3	1.8278377867077809E-4	0.0	0.0	1.0314488630392007	0.0
4	1.8278377867077809E-4	0.0	0.0	1.4564211484487597	0.0
5	1.8278377867077809E-4	0.0	0.0	3.1230436423689145	0.0
6	1.8278377867077809E-4	0.0	0.0	3.6708009311005685	0.0
7	1.8278377867077809E-4	0.0	0.0	4.314976663081058	0.0
8	1.8278377867077809E-4	0.0	0.0	5.1733749836637	0.0
9	1.8278377867077809E-4	0.0	0.0	5.447870523282541	0.0
10	1.8278377867077809E-4	0.0	0.0	6.939249069402087	0.0
11	1.8278377867077809E-4	0.0	0.0	7.9119786435433	0.0
12	1.8278377867077809E-4	0.0	0.0	9.711027985110434	0.0
13	1.8278377867077809E-4	0.0	0.0	10.05438731334349	0.0
14	1.8278377867077809E-4	0.0	0.0	10.209022390098967	0.0
15	1.8278377867077809E-4	0.0	0.0	10.582449649923367	0.0
16	1.8278377867077809E-4	0.0	0.0	11.104525817751778	0.0
17	1.8278377867077809E-4	0.0	0.0	11.718039570860244	0.0
18	2.284797233384726E-4	0.0	0.0	12.312680898820954	0.0
19	2.7417566800616713E-4	0.0	0.0	12.975409184336527	0.0
20	2.7417566800616713E-4	0.0	0.0	13.420624773233875	0.0
21	2.7417566800616713E-4	0.0	0.0	13.941649934334928	0.0
22	2.7417566800616713E-4	0.0	0.0	14.561149856194863	0.0
23	2.7417566800616713E-4	0.0	0.0	15.109592584096532	0.0
24	2.7417566800616713E-4	0.0	0.0	15.555310828385224	0.0
25	2.7417566800616713E-4	0.0	0.0	15.937374621751818	0.0
26	2.7417566800616713E-4	0.0	0.0	16.295585132001875	0.0
27	3.198716126738616E-4	0.0	0.0	16.68994113448408	0.0
28	3.6556755734155617E-4	0.0	0.0	17.059667022790396	0.0
29	4.1126350200925067E-4	0.0	0.0	17.446026234955752	0.0
30	4.1126350200925067E-4	0.0	0.0	17.874105844602713	0.0
31	4.1126350200925067E-4	0.0	0.0	18.264988955290175	0.0
32	4.1126350200925067E-4	0.0	0.0	18.654364099803598	0.0
33	4.1126350200925067E-4	0.0	0.0	19.0218508868212	0.0
34	4.569594466769452E-4	0.0	0.0	19.397060288487637	0.0
35	4.569594466769452E-4	0.0	0.0	19.78488177088236	0.0
36	4.569594466769452E-4	0.0	0.0	20.18111130709594	0.0
37	4.569594466769452E-4	0.0	0.0	20.58785091058309	0.0
38	4.569594466769452E-4	0.0	0.0	20.983989054907333	0.0
39	4.569594466769452E-4	0.0	0.0	21.400827462166042	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTC	20	7.0346874E-4	45.0	12
CCTATCG	20	7.0346874E-4	45.0	1
CGATCGA	185	0.0	42.567566	41
TACGGCT	2355	0.0	41.847134	7
ACGATCG	60	3.6379788E-12	41.250004	40
GTACGAG	180	0.0	40.0	1
CCGATCG	175	0.0	39.857143	40
TCTACGG	130	0.0	39.807693	2
CCGATGA	2310	0.0	39.545456	18
CGTAATG	40	3.4601362E-7	39.375	34
ACCCGTA	40	3.4601362E-7	39.375	31
CGATGAA	2380	0.0	38.94958	19
TGCGTAG	185	0.0	38.91892	1
ACCACCG	3230	0.0	38.86997	14
ACTCGAT	35	6.250879E-6	38.571426	13
CACGTTA	35	6.250879E-6	38.571426	31
CCACCGA	3020	0.0	38.145695	15
GGACGTA	130	0.0	38.076927	8
ACGGCTG	2600	0.0	38.076923	8
TGATACC	2255	0.0	38.01552	4
>>END_MODULE