##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934861.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	872125
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24032105489465	33.0	31.0	34.0	30.0	34.0
2	32.41267249534184	34.0	31.0	34.0	30.0	34.0
3	32.40738426257704	34.0	31.0	34.0	30.0	34.0
4	35.95723090153361	37.0	35.0	37.0	35.0	37.0
5	35.85908671348717	37.0	35.0	37.0	35.0	37.0
6	35.78961387415795	37.0	35.0	37.0	35.0	37.0
7	36.12003325211409	37.0	35.0	37.0	35.0	37.0
8	35.97176322201519	37.0	35.0	37.0	35.0	37.0
9	37.66034226745019	39.0	38.0	39.0	35.0	39.0
10	37.37221499211696	39.0	37.0	39.0	34.0	39.0
11	37.228949978500786	39.0	37.0	39.0	34.0	39.0
12	37.130734699727675	39.0	37.0	39.0	34.0	39.0
13	37.03123405475132	39.0	37.0	39.0	33.0	39.0
14	38.19087630786871	40.0	37.0	41.0	33.0	41.0
15	38.26798910706607	40.0	37.0	41.0	34.0	41.0
16	38.370327647986244	40.0	37.0	41.0	34.0	41.0
17	38.37816712053891	40.0	37.0	41.0	34.0	41.0
18	38.34623706464097	40.0	37.0	41.0	34.0	41.0
19	38.320507954708326	40.0	37.0	41.0	34.0	41.0
20	38.243711623907124	40.0	37.0	41.0	34.0	41.0
21	38.18009918302995	40.0	37.0	41.0	34.0	41.0
22	38.11575290239358	40.0	36.0	41.0	34.0	41.0
23	37.973101332951124	40.0	36.0	41.0	34.0	41.0
24	37.69406335101046	40.0	36.0	41.0	33.0	41.0
25	37.63427031675505	39.0	36.0	41.0	33.0	41.0
26	37.78727475992547	40.0	36.0	41.0	34.0	41.0
27	37.825629353590365	40.0	36.0	41.0	34.0	41.0
28	37.8266922746166	40.0	36.0	41.0	34.0	41.0
29	37.83804500501648	40.0	36.0	41.0	34.0	41.0
30	37.69905346137308	40.0	36.0	41.0	33.0	41.0
31	37.58760728106636	40.0	36.0	41.0	33.0	41.0
32	37.40880263723663	40.0	35.0	41.0	33.0	41.0
33	37.294929052601404	39.0	35.0	41.0	33.0	41.0
34	37.27522430844203	40.0	35.0	41.0	33.0	41.0
35	37.07842912426544	39.0	35.0	41.0	32.0	41.0
36	37.01435344704028	39.0	35.0	41.0	31.0	41.0
37	36.954223305145476	39.0	35.0	41.0	31.0	41.0
38	36.86902909559983	39.0	35.0	41.0	31.0	41.0
39	36.96534671062061	39.0	35.0	41.0	32.0	41.0
40	36.867299985667195	39.0	35.0	41.0	31.0	41.0
41	36.69094223878458	39.0	35.0	41.0	31.0	41.0
42	36.606856815250104	39.0	35.0	41.0	31.0	41.0
43	36.60508872008026	39.0	35.0	41.0	31.0	41.0
44	36.58233739429554	39.0	35.0	41.0	31.0	41.0
45	36.548325068080835	39.0	35.0	41.0	31.0	41.0
46	36.454212412211554	39.0	35.0	41.0	31.0	41.0
47	36.30000859968468	39.0	35.0	41.0	30.0	41.0
48	36.232388705747454	39.0	35.0	41.0	30.0	41.0
49	36.23312483875591	39.0	35.0	40.0	30.0	41.0
50	36.14122631503511	39.0	35.0	40.0	30.0	41.0
51	35.19783402608571	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	9.0
11	11.0
12	11.0
13	10.0
14	16.0
15	24.0
16	42.0
17	91.0
18	203.0
19	326.0
20	646.0
21	1020.0
22	1491.0
23	2305.0
24	3262.0
25	4732.0
26	6679.0
27	8031.0
28	8904.0
29	10091.0
30	12294.0
31	15191.0
32	20295.0
33	28437.0
34	53297.0
35	74370.0
36	62977.0
37	99953.0
38	182482.0
39	274684.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43958721513544	21.370560412784865	24.90491615307439	12.284936219005303
2	33.339486885480866	23.54742726100043	24.962706034112085	18.150379819406623
3	30.77104772824996	23.923176150207826	28.260054464669626	17.04572165687258
4	28.482155654292672	25.280550379819406	26.959094166547224	19.27819979934069
5	24.829697577755482	28.701963594668197	26.529339257560558	19.938999570015767
6	26.110018632650135	32.722832162820694	26.06300702307582	15.104142181453348
7	80.73051454779991	4.95295972481009	9.08470689408055	5.231818833309446
8	81.6354307008743	3.654636663322345	8.920280923032822	5.789651712770532
9	76.10078830442883	6.199684678228465	11.17752615737423	6.522000859968467
10	38.997620753905686	28.607367063207683	18.788132435144046	13.606879747742584
11	26.575777554822988	24.79552816396732	28.688662749032535	19.940031532177155
12	25.80329654579332	22.114490468682817	31.95402035258707	20.12819263293679
13	22.360326788017773	25.853518704314176	32.050566145907986	19.73558836176007
14	17.485681525010747	29.20934499068367	30.83491471979361	22.47005876451197
15	16.53432707467393	27.533323778128132	36.82774831589508	19.10460083130285
16	19.280951698437722	25.80203525870718	33.861659739142894	21.055353303712195
17	19.28519420954565	25.640246524294106	29.55126845349004	25.5232908126702
18	19.611294252544074	26.282700300988964	32.93725096746453	21.168754479002434
19	21.64460369786441	26.88341694137882	29.893191916296402	21.57878744446037
20	22.90256557259567	27.569786441163824	30.35344704027519	19.174200945965314
21	22.38291529310592	26.81897663752329	30.54504801490612	20.25306005446467
22	19.72882327647986	25.73461373082987	29.500358320194923	25.036204672495344
23	18.8529167263867	26.82918159667479	30.230643543070084	24.087258133868424
24	21.299240361186754	24.830958864841623	31.29207395728823	22.577726816683388
25	19.443772395012182	27.804041851798765	29.054550666475564	23.697635086713486
26	20.59567149204529	28.53776694854522	28.685108212698868	22.18145334671062
27	20.25810520280923	28.037838612584203	30.483932922459513	21.220123262147055
28	17.610319621613872	26.415020782571307	33.90993263580335	22.064726960011466
29	20.757689551383116	23.62780564712627	33.09823706464096	22.51626773684965
30	23.347685251540774	24.839558549519854	30.790655009316325	21.022101189623047
31	23.257445893650566	26.409631646839614	28.682356313601836	21.650566145907984
32	24.901246954278346	25.948000573312314	28.429296259137164	20.72145621327218
33	24.197448760212126	24.93335244374373	29.856614590798337	21.01258420524581
34	22.488175433567438	23.922832162820697	31.341722803497206	22.247269600114663
35	22.444259710477283	23.343901390282358	30.980421384549235	23.231417514691127
36	24.19664612297549	23.7276766518561	31.939687544790026	20.135989680378387
37	21.74034685394869	24.314060484448905	34.37248100902967	19.573111652572738
38	21.69310591944962	24.414619463952988	31.38850508814677	22.503769528450622
39	24.729023935789023	23.676193206249106	30.676909846638956	20.917873011322918
40	25.714547799914	22.66028378959438	30.938225598394727	20.68694281209689
41	21.00203525870718	23.331861831732837	31.872839329224593	23.79326358033539
42	22.24979217428694	22.3398022072524	32.83416941378816	22.576236204672494
43	23.173168983803926	23.205274473269313	31.99541350150494	21.626143041421813
44	22.198079403755195	24.62296115809087	29.53567435860685	23.643285079547084
45	23.45053748029239	24.02499641679805	27.51612440877168	25.008341694137883
46	24.54132148487889	24.043801060627775	29.992145621327214	21.422731833166118
47	21.051684104916152	24.22359180163394	33.43282212985524	21.29190196359467
48	21.33134585065214	22.01003296545793	33.15396302135588	23.50465816253404
49	22.448846208972338	21.246724953418376	34.136620323921456	22.16780851368783
50	21.98480722373513	20.91500644976351	33.207166403898526	23.893019922602836
51	21.039529883904258	21.56800917299699	29.448530887200803	27.943930055897955
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	515.5
2	731.0
3	3216.5
4	5702.0
5	3897.0
6	2092.0
7	2044.0
8	1996.0
9	2086.5
10	2177.0
11	2141.5
12	2106.0
13	2069.0
14	2032.0
15	1933.5
16	1835.0
17	2131.0
18	2427.0
19	2451.5
20	2476.0
21	2795.0
22	3114.0
23	3607.5
24	4101.0
25	4926.5
26	7297.5
27	8843.0
28	10405.0
29	11967.0
30	12661.0
31	13355.0
32	16346.0
33	19337.0
34	23164.0
35	26991.0
36	28094.5
37	29198.0
38	30791.5
39	32385.0
40	35946.0
41	39507.0
42	45206.0
43	50905.0
44	57964.0
45	65023.0
46	72835.0
47	80647.0
48	92081.0
49	103515.0
50	102012.0
51	100509.0
52	84378.5
53	68248.0
54	56671.5
55	45095.0
56	39779.0
57	34463.0
58	30405.0
59	26347.0
60	25254.5
61	24162.0
62	21239.0
63	18316.0
64	16078.0
65	13840.0
66	11321.0
67	8802.0
68	7124.5
69	5447.0
70	4467.0
71	3487.0
72	2944.5
73	2402.0
74	1769.5
75	909.0
76	681.0
77	491.5
78	302.0
79	304.5
80	307.0
81	172.0
82	37.0
83	28.5
84	20.0
85	12.5
86	5.0
87	4.5
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	872125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.72594263341111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.00526123484461	16.134889268280595
2	12.747236627360357	6.048804099105374
3	5.6128880979334745	3.995131830579768
4	3.0205241062784043	2.866591266735871
5	1.8488776832253135	2.1933182924198915
6	1.2035008635102031	1.7132515468142286
7	0.8614662924830396	1.4307369885248968
8	0.6005520185972102	1.139893019329332
9	0.44310653597543675	0.9461808227738455
>10	3.5630895970060172	19.471151072048055
>50	1.69315471026023	29.28780416719579
>100	0.3939953434734727	12.062627648839232
>500	0.002929333408724704	0.4773573840061695
>1k	0.0034175556435121547	2.2322625933469773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	4731	0.5424681095026516	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	4326	0.49602981224021786	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3269	0.3748315895083847	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	2727	0.31268453490038695	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1705	0.19549949835172709	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1296	0.14860255123978786	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	1217	0.1395442167120539	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	875	0.1003296545793321	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2932492475275906E-4	0.0	0.0	0.33424107782714635	0.0
2	2.2932492475275906E-4	0.0	0.0	1.8198079403755196	0.0
3	2.2932492475275906E-4	0.0	0.0	2.5553676365199944	0.0
4	2.2932492475275906E-4	0.0	0.0	3.3001003296545792	0.0
5	2.2932492475275906E-4	0.0	0.0	5.295571162390712	0.0
6	2.2932492475275906E-4	0.0	0.0	6.219291959294826	0.0
7	2.2932492475275906E-4	0.0	0.0	7.199197362763365	0.0
8	2.2932492475275906E-4	0.0	0.0	8.683846925612727	0.0
9	2.2932492475275906E-4	0.0	0.0	9.208771678371793	0.0
10	3.439873871291386E-4	0.0	0.0	10.469829439587215	0.0
11	3.439873871291386E-4	0.0	0.0	12.435029382255983	0.0
12	3.439873871291386E-4	0.0	0.0	13.902938225598394	0.0
13	3.439873871291386E-4	0.0	0.0	14.485652859395156	0.0
14	3.439873871291386E-4	0.0	0.0	14.728622617170704	0.0
15	3.439873871291386E-4	0.0	0.0	15.165945248674216	0.0
16	3.439873871291386E-4	0.0	0.0	16.096775118245663	0.0
17	3.439873871291386E-4	0.0	0.0	17.264382972624336	0.0
18	3.439873871291386E-4	0.0	0.0	18.503998853375375	0.0
19	3.439873871291386E-4	0.0	0.0	19.2145621327218	0.0
20	3.439873871291386E-4	0.0	0.0	19.9024222445177	0.0
21	3.439873871291386E-4	0.0	0.0	20.737394295542497	0.0
22	3.439873871291386E-4	0.0	0.0	21.664669628780278	0.0
23	3.439873871291386E-4	0.0	0.0	22.561674071950694	0.0
24	3.439873871291386E-4	0.0	0.0	23.24815823419808	0.0
25	3.439873871291386E-4	0.0	0.0	23.859538483588935	0.0
26	3.439873871291386E-4	0.0	0.0	24.36932779131432	0.0
27	3.439873871291386E-4	0.0	0.0	24.89344990683675	0.0
28	3.439873871291386E-4	0.0	0.0	25.426974344274043	0.0
29	3.439873871291386E-4	0.0	0.0	25.994094883187618	0.0
30	3.439873871291386E-4	0.0	0.0	26.6825283072954	0.0
31	3.439873871291386E-4	0.0	0.0	27.292876594524866	0.0
32	3.439873871291386E-4	0.0	0.0	27.840504514834457	0.0
33	3.439873871291386E-4	0.0	0.0	28.345363336677654	0.0
34	3.439873871291386E-4	0.0	0.0	28.89253260713774	0.0
35	3.439873871291386E-4	0.0	0.0	29.470316755052316	0.0
36	3.439873871291386E-4	0.0	0.0	30.019435287372797	0.0
37	3.439873871291386E-4	0.0	0.0	30.55605561129425	0.0
38	3.439873871291386E-4	0.0	0.0	31.074329941235487	0.0
39	0.0010319621613874158	0.0	0.0	31.60567579188763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.032348E-4	45.000004	22
TGCCCGT	20	7.032348E-4	45.000004	26
ACACGGT	35	1.2114833E-7	45.000004	34
CGACGAT	20	7.032348E-4	45.000004	27
CTATCGA	20	7.032348E-4	45.000004	43
CGGCATA	20	7.032348E-4	45.000004	39
GCTATCG	20	7.032348E-4	45.000004	14
TAACGCC	20	7.032348E-4	45.000004	12
CGTTCCC	20	7.032348E-4	45.000004	31
TCGGCAC	20	7.032348E-4	45.000004	32
ATCGTGC	20	7.032348E-4	45.000004	34
GTGGACG	20	7.032348E-4	45.000004	1
CGTTAGC	20	7.032348E-4	45.000004	29
CTTATCG	45	3.8380676E-10	45.000004	12
TCCGTTC	20	7.032348E-4	45.000004	23
CTCGCGT	20	7.032348E-4	45.000004	25
TTAACGG	30	2.1648957E-6	45.000004	2
TCCGTAC	20	7.032348E-4	45.000004	32
CGTAACG	35	1.2114833E-7	45.000004	13
CCGCGAA	20	7.032348E-4	45.000004	34
>>END_MODULE