##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934860.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	484562
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2520688786987	33.0	31.0	34.0	30.0	34.0
2	32.43561814587194	34.0	31.0	34.0	30.0	34.0
3	32.44324152533628	34.0	31.0	34.0	30.0	34.0
4	35.98688506321172	37.0	35.0	37.0	35.0	37.0
5	35.8632022321189	37.0	35.0	37.0	35.0	37.0
6	35.76733214738258	37.0	35.0	37.0	35.0	37.0
7	36.00509945063789	37.0	35.0	37.0	35.0	37.0
8	35.659896979127545	37.0	35.0	37.0	35.0	37.0
9	37.43032883304923	39.0	37.0	39.0	35.0	39.0
10	37.2993321804021	39.0	37.0	39.0	34.0	39.0
11	37.17990267499309	39.0	37.0	39.0	34.0	39.0
12	36.948941518319636	39.0	35.0	39.0	33.0	39.0
13	36.762618612272526	39.0	35.0	39.0	33.0	39.0
14	37.88494970715822	40.0	36.0	41.0	33.0	41.0
15	37.93016579921661	40.0	36.0	41.0	33.0	41.0
16	38.140378321040444	40.0	36.0	41.0	34.0	41.0
17	38.039943288990884	40.0	36.0	41.0	33.0	41.0
18	38.06682736161729	40.0	36.0	41.0	34.0	41.0
19	38.015420111358296	40.0	36.0	41.0	34.0	41.0
20	37.79432972457601	40.0	36.0	41.0	33.0	41.0
21	37.83592811652585	40.0	35.0	41.0	33.0	41.0
22	37.730463800298004	40.0	35.0	41.0	33.0	41.0
23	37.48889306218812	39.0	35.0	41.0	33.0	41.0
24	37.28381301051259	39.0	35.0	41.0	32.0	41.0
25	37.31263904309459	39.0	35.0	41.0	32.0	41.0
26	37.409518286617605	39.0	35.0	41.0	33.0	41.0
27	37.38942385081785	39.0	35.0	41.0	33.0	41.0
28	37.329334533042214	39.0	35.0	41.0	33.0	41.0
29	37.31215406903554	39.0	35.0	41.0	33.0	41.0
30	37.17876762932298	39.0	35.0	41.0	32.0	41.0
31	37.18362562479105	39.0	35.0	41.0	32.0	41.0
32	36.89464093346156	39.0	35.0	41.0	31.0	41.0
33	36.78194534445541	39.0	35.0	41.0	31.0	41.0
34	36.705742505603	39.0	35.0	41.0	31.0	41.0
35	36.55541499333419	39.0	35.0	41.0	31.0	41.0
36	36.53184731778389	39.0	35.0	41.0	31.0	41.0
37	36.37437933639039	39.0	35.0	41.0	30.0	41.0
38	36.302229642439976	39.0	35.0	41.0	30.0	41.0
39	36.3395272431598	39.0	35.0	41.0	30.0	41.0
40	36.23694181549523	39.0	35.0	41.0	30.0	41.0
41	36.188200890701296	39.0	35.0	41.0	30.0	41.0
42	36.14288780383109	39.0	35.0	41.0	30.0	41.0
43	36.04243832574573	39.0	35.0	41.0	30.0	41.0
44	36.051883969440446	39.0	35.0	41.0	30.0	41.0
45	36.00322972086131	38.0	35.0	41.0	29.0	41.0
46	35.98285049178433	38.0	35.0	40.0	30.0	41.0
47	35.795726862609946	38.0	35.0	40.0	29.0	41.0
48	35.73012328659697	38.0	35.0	40.0	29.0	41.0
49	35.72894077538065	38.0	35.0	40.0	29.0	41.0
50	35.607437231974444	38.0	35.0	40.0	29.0	41.0
51	34.76493204172015	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	19.0
10	9.0
11	9.0
12	11.0
13	11.0
14	13.0
15	26.0
16	46.0
17	71.0
18	162.0
19	366.0
20	664.0
21	1257.0
22	2062.0
23	2552.0
24	3127.0
25	3822.0
26	4525.0
27	5104.0
28	5355.0
29	6174.0
30	7497.0
31	9230.0
32	12509.0
33	17374.0
34	32582.0
35	46146.0
36	33847.0
37	51671.0
38	92960.0
39	145231.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.043358744598216	20.23229225568658	22.28177199202579	12.442577007689419
2	30.303036556725456	30.246903389039996	22.315616990189078	17.134443064045467
3	29.368584412314625	30.12081013368774	24.61790235305286	15.892703100944772
4	27.444372443567595	23.89126675224223	31.209834861173597	17.45452594301658
5	29.686603571885538	27.381222629921453	23.395974096194088	19.536199701998918
6	25.706927080538712	36.92179741704879	23.024710976098	14.346564526314488
7	75.05499812201535	5.749728620898874	14.622071066241265	4.573202190844515
8	75.53790846166228	10.355124834386519	8.990799938913906	5.116166765037292
9	70.5719804689596	6.693880246490645	9.73373892298612	13.000400361563639
10	44.110557575707546	23.802733189973626	18.205100688869535	13.881608545449293
11	35.549424015915406	23.555293233889575	22.741568674390482	18.15371407580454
12	29.292433166447225	21.328746373013153	27.863101109868293	21.51571935067133
13	26.12132193609899	24.378304530689572	31.072803893000277	18.42756964021116
14	20.183794849781865	31.98744433116918	26.580293130703602	21.24846768834535
15	17.715792819081972	25.367032495325674	36.08227636504719	20.834898320545154
16	19.33251059719912	24.388623127690572	30.972094386270488	25.30677188883982
17	20.143139577597914	25.49766593335837	29.631295891960164	24.727898597083552
18	21.084401996029403	25.066142206776433	29.102364609688752	24.74709118750542
19	22.438201922560992	27.3950495499028	26.47442432547331	23.692324202062895
20	26.429435242548944	25.344537953863487	29.047469673643416	19.178557129944156
21	23.30682141810542	29.212360853719442	27.539922651796882	19.940895076378254
22	21.461237158506034	23.42919997853732	29.808775760377415	25.300787102579235
23	22.38929177277624	27.53269963389618	27.796030229361772	22.281978363965806
24	22.208509953318668	25.191616346308628	29.0575818987044	23.54229180166831
25	19.512260556956594	30.52963294686748	27.703162856352748	22.25494363982318
26	20.538548214676347	26.42757789508876	29.186151617336893	23.847722272897997
27	22.727329010529097	28.427734737763178	27.36182366755957	21.48311258414816
28	19.664769420631416	27.630932677345726	31.470069877538894	21.23422802448397
29	23.382559920092785	25.05025156739488	29.28108270974612	22.28610580276621
30	21.36032127983622	28.9958766886384	28.938711661252842	20.705090370272536
31	26.94701606811925	25.49745956141835	23.796542031773026	23.75898233868937
32	25.985116455685752	29.350423681592865	23.783127855671722	20.881332007049664
33	23.65497088091926	27.660031120888558	25.166851713506215	23.518146284685965
34	26.486187526054454	25.883375089255868	24.844705115134904	22.785732269554774
35	22.571105451933914	24.357460964747546	27.225824559086348	25.845609024232193
36	25.96592386526389	26.429228870608924	26.69503592935476	20.90981133477243
37	22.069828009625187	27.065886305570803	29.848192800921243	21.016092883882763
38	22.103053891968415	29.144464485452843	24.881852064338517	23.870629558240225
39	25.28778567035797	27.667254138789254	25.54657608314313	21.498384107709644
40	22.421279423479348	25.775648936565393	27.85629083584763	23.946780804107625
41	22.28713766246631	24.544640314345738	27.91840878979367	25.24981323339428
42	21.456077860005532	24.088351954961386	28.563527474296375	25.892042710736707
43	24.120339605664498	24.06007899917864	27.976812048819344	23.842769346337516
44	23.037918780259286	25.32740908284182	27.949983696616737	23.68468844028215
45	21.510353680230807	25.349490880423968	25.96881307242417	27.171342366921053
46	25.399845633788864	26.744565194959574	25.743867657802305	22.111721513449258
47	20.32990618331607	25.005056112530493	32.630705668211704	22.034332035941738
48	22.458632744622978	24.054713328738117	28.58251369277822	24.904140233860684
49	21.51964041753171	22.123691085970425	32.64370710043297	23.712961396064898
50	21.5586447141955	23.46614055580091	29.848399172861264	25.12681555714233
51	21.27034311398748	23.420738728996497	26.35122027728134	28.95769787973469
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	208.0
1	326.0
2	444.0
3	1783.5
4	3123.0
5	2130.0
6	1137.0
7	1149.5
8	1162.0
9	1123.5
10	1085.0
11	1082.5
12	1080.0
13	1062.5
14	1045.0
15	1007.0
16	969.0
17	950.5
18	932.0
19	980.5
20	1029.0
21	1170.0
22	1311.0
23	1390.0
24	1469.0
25	1933.5
26	2477.5
27	2557.0
28	4296.5
29	6036.0
30	6385.5
31	6735.0
32	8160.0
33	9585.0
34	10438.0
35	11291.0
36	11753.0
37	12215.0
38	13107.0
39	13999.0
40	17114.5
41	20230.0
42	23274.0
43	26318.0
44	30010.0
45	33702.0
46	47023.5
47	60345.0
48	54206.0
49	48067.0
50	47259.5
51	46452.0
52	40356.5
53	34261.0
54	30614.0
55	26967.0
56	24994.5
57	23022.0
58	21674.0
59	20326.0
60	19635.0
61	18944.0
62	17780.0
63	16616.0
64	13234.0
65	9852.0
66	8878.5
67	7905.0
68	6020.0
69	4135.0
70	3391.0
71	2647.0
72	2253.5
73	1860.0
74	1619.5
75	1053.0
76	727.0
77	630.0
78	533.0
79	450.0
80	367.0
81	216.0
82	65.0
83	43.5
84	22.0
85	13.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	484562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.048247192080485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77582499512773	17.741792762825018
2	10.80435263133848	5.19651485657666
3	4.1332889988339785	2.9819506668079914
4	2.094827202108116	2.0150768952396128
5	1.2197350329093537	1.466624479012228
6	0.7825711990464411	1.129167938404298
7	0.5576084677203305	0.9386654387695016
8	0.39213365916657233	0.7544101734378215
9	0.32404859416770737	0.7013520625312077
>10	3.889444119661912	23.74440982633344
>50	1.8081218118378994	30.46880774823763
>100	0.20597765158687226	6.319835622280033
>500	0.005170987069126499	0.8822857785080543
>1k	0.00430915589093875	1.6949337788674814
>5k	0.0025854935345632495	3.964171972169029
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	7137	1.4728765359231635	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	6298	1.299730478246334	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	5692	1.174669082594178	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	2371	0.48930786978756075	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.3805498573969895	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAAGCTT	1411	0.2911908073683038	No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	1351	0.2788084909671002	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	1201	0.2478526999640913	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTA	843	0.17397154543691	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	814	0.16798675917632833	No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT	729	0.15044514427462327	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	715	0.14755593711434242	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	643	0.13269715743289817	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	513	0.10586880523029045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0637194002005936E-4	0.0	0.0	0.15766816217532534	0.0
2	2.0637194002005936E-4	0.0	0.0	1.178796521394579	0.0
3	2.0637194002005936E-4	0.0	0.0	1.578951713093474	0.0
4	2.0637194002005936E-4	0.0	0.0	2.1716519248310844	0.0
5	2.0637194002005936E-4	0.0	0.0	4.574646794424655	0.0
6	2.0637194002005936E-4	0.0	0.0	5.17911020674341	0.0
7	2.0637194002005936E-4	0.0	0.0	6.049380677808	0.0
8	2.0637194002005936E-4	0.0	0.0	7.0818594937283565	0.0
9	2.0637194002005936E-4	0.0	0.0	7.387909080778105	0.0
10	2.0637194002005936E-4	0.0	0.0	9.497236679723132	0.0
11	2.0637194002005936E-4	0.0	0.0	10.656221494875785	0.0
12	2.0637194002005936E-4	0.0	0.0	13.17499102282061	0.0
13	2.0637194002005936E-4	0.0	0.0	13.609197584622814	0.0
14	2.0637194002005936E-4	0.0	0.0	13.798027909741169	0.0
15	2.0637194002005936E-4	0.0	0.0	14.28939949892893	0.0
16	2.0637194002005936E-4	0.0	0.0	14.939058366112077	0.0
17	2.0637194002005936E-4	0.0	0.0	15.667757686322906	0.0
18	2.0637194002005936E-4	0.0	0.0	16.449288223178872	0.0
19	2.0637194002005936E-4	0.0	0.0	17.243613820316078	0.0
20	2.0637194002005936E-4	0.0	0.0	17.726524159963017	0.0
21	2.0637194002005936E-4	0.0	0.0	18.25586818611447	0.0
22	2.0637194002005936E-4	0.0	0.0	18.858474250973043	0.0
23	2.0637194002005936E-4	0.0	0.0	19.431775500348767	0.0
24	2.0637194002005936E-4	0.0	0.0	19.903748127174644	0.0
25	2.0637194002005936E-4	0.0	0.0	20.3096817331941	0.0
26	2.0637194002005936E-4	0.0	0.0	20.671864487929305	0.0
27	2.0637194002005936E-4	0.0	0.0	21.09905440377083	0.0
28	2.0637194002005936E-4	0.0	0.0	21.473825846847255	0.0
29	2.0637194002005936E-4	0.0	0.0	21.852105612904026	0.0
30	2.0637194002005936E-4	0.0	0.0	22.274548974125086	0.0
31	2.0637194002005936E-4	0.0	0.0	22.686880110285166	0.0
32	2.0637194002005936E-4	0.0	0.0	23.0853843264639	0.0
33	2.0637194002005936E-4	0.0	0.0	23.47584003698185	0.0
34	2.0637194002005936E-4	0.0	0.0	23.833276237096594	0.0
35	2.0637194002005936E-4	0.0	0.0	24.228891246115047	0.0
36	2.0637194002005936E-4	0.0	0.0	24.61687049335276	0.0
37	2.0637194002005936E-4	0.0	0.0	24.989990960909026	0.0
38	2.0637194002005936E-4	0.0	0.0	25.337521307902808	0.0
39	2.0637194002005936E-4	0.0	0.0	25.707958940238814	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGT	20	7.029242E-4	45.000004	17
CGAACCG	20	7.029242E-4	45.000004	24
ATTAGCG	20	7.029242E-4	45.000004	1
TAGCGGA	20	7.029242E-4	45.000004	45
TAGCGAG	45	3.8380676E-10	45.000004	1
TAGCGAA	20	7.029242E-4	45.000004	13
TTGTCGT	20	7.029242E-4	45.000004	36
CGACCGT	20	7.029242E-4	45.000004	22
CCGTGAC	20	7.029242E-4	45.000004	10
CGATTTA	20	7.029242E-4	45.000004	27
ATCGTCG	20	7.029242E-4	45.000004	20
GTAGCGA	20	7.029242E-4	45.000004	12
TGACCTC	20	7.029242E-4	45.000004	22
GCGGGTT	40	6.8012014E-9	45.000004	5
AGACCGA	20	7.029242E-4	45.000004	20
GTCCGTC	20	7.029242E-4	45.000004	38
ACTAAGC	20	7.029242E-4	45.000004	10
TTACGGA	20	7.029242E-4	45.000004	34
TCGAGTT	20	7.029242E-4	45.000004	37
GGCGCTA	20	7.029242E-4	45.000004	1
>>END_MODULE