##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934859.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1162913
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.372481862357716	33.0	31.0	34.0	31.0	34.0
2	32.54773056969868	34.0	31.0	34.0	31.0	34.0
3	32.5422882021269	34.0	31.0	34.0	31.0	34.0
4	36.057498712285444	37.0	35.0	37.0	35.0	37.0
5	35.957721686832976	37.0	35.0	37.0	35.0	37.0
6	35.88582464896342	37.0	35.0	37.0	35.0	37.0
7	36.11958676186439	37.0	35.0	37.0	35.0	37.0
8	35.89506695685748	37.0	35.0	37.0	35.0	37.0
9	37.55075831124083	39.0	38.0	39.0	35.0	39.0
10	37.40424864112793	39.0	37.0	39.0	35.0	39.0
11	37.334827282866385	39.0	37.0	39.0	35.0	39.0
12	37.195330175172174	39.0	37.0	39.0	34.0	39.0
13	37.14319557868903	39.0	37.0	39.0	33.0	39.0
14	38.33089577638224	40.0	38.0	41.0	34.0	41.0
15	38.401861532204045	40.0	38.0	41.0	34.0	41.0
16	38.50920232209976	40.0	38.0	41.0	34.0	41.0
17	38.46661272167393	40.0	38.0	41.0	34.0	41.0
18	38.44053080496993	40.0	38.0	41.0	34.0	41.0
19	38.424104812655806	40.0	37.0	41.0	34.0	41.0
20	38.336523884417836	40.0	37.0	41.0	34.0	41.0
21	38.28823566337292	40.0	37.0	41.0	34.0	41.0
22	38.22988048117099	40.0	37.0	41.0	34.0	41.0
23	38.072020864845435	40.0	37.0	41.0	34.0	41.0
24	37.833247199059606	40.0	36.0	41.0	33.0	41.0
25	37.75577106799907	40.0	36.0	41.0	33.0	41.0
26	37.8741075213709	40.0	36.0	41.0	34.0	41.0
27	37.92768504608685	40.0	36.0	41.0	34.0	41.0
28	37.850148721357485	40.0	36.0	41.0	33.0	41.0
29	37.845314309840894	40.0	36.0	41.0	34.0	41.0
30	37.74008029835422	40.0	36.0	41.0	33.0	41.0
31	37.66933295955931	40.0	36.0	41.0	33.0	41.0
32	37.57597085938501	40.0	36.0	41.0	33.0	41.0
33	37.458225163877266	40.0	36.0	41.0	33.0	41.0
34	37.388624944428344	40.0	36.0	41.0	33.0	41.0
35	37.3163744837318	40.0	35.0	41.0	33.0	41.0
36	37.22168296338591	40.0	35.0	41.0	32.0	41.0
37	37.16213250690292	40.0	35.0	41.0	32.0	41.0
38	37.07036467904306	40.0	35.0	41.0	32.0	41.0
39	37.05421299787688	39.0	35.0	41.0	32.0	41.0
40	36.9670938410698	39.0	35.0	41.0	32.0	41.0
41	36.88734496905615	39.0	35.0	41.0	31.0	41.0
42	36.81294817411105	39.0	35.0	41.0	31.0	41.0
43	36.75892177660754	39.0	35.0	41.0	31.0	41.0
44	36.700645706084636	39.0	35.0	41.0	31.0	41.0
45	36.65906907911426	39.0	35.0	41.0	31.0	41.0
46	36.59786071700979	39.0	35.0	41.0	31.0	41.0
47	36.462022524470875	39.0	35.0	41.0	31.0	41.0
48	36.403318218989725	39.0	35.0	41.0	31.0	41.0
49	36.33789458024805	39.0	35.0	41.0	31.0	41.0
50	36.24827136681764	38.0	35.0	40.0	31.0	41.0
51	35.29977392977806	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	14.0
10	23.0
11	18.0
12	20.0
13	28.0
14	39.0
15	38.0
16	84.0
17	151.0
18	295.0
19	493.0
20	944.0
21	1545.0
22	2276.0
23	3221.0
24	4453.0
25	6256.0
26	8255.0
27	9936.0
28	10915.0
29	12608.0
30	15289.0
31	19143.0
32	25191.0
33	35777.0
34	68490.0
35	91037.0
36	83311.0
37	129165.0
38	236189.0
39	397429.0
40	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.07795424077296	21.654328397739125	24.295712576951157	11.972004784536763
2	32.714829054280074	25.058280370070673	25.10385557647047	17.123034999178785
3	31.991301154944523	25.206270804436787	26.014671776822514	16.787756263796176
4	29.178537001478183	25.152612448222694	26.855749312287337	18.813101238011786
5	26.906483976015405	29.616058982916176	23.73591145683297	19.74154558423545
6	26.621853913405385	35.214930093652754	23.434943112683406	14.728272880258455
7	81.25242387005734	5.569806167787272	8.723094504919974	4.454675457235409
8	81.72640601661517	5.959775150849634	7.639522475026077	4.674296357509117
9	75.74074758816867	7.578984842374278	9.283067606949102	7.397199962507943
10	40.6038112911284	28.104681949552546	16.900490406419053	14.391016352900001
11	31.53099157030664	25.388055684303122	23.962927579277213	19.11802516611303
12	28.770595908722317	22.265896073051035	28.52655357709476	20.43695444113188
13	25.342050523125977	24.2952826221738	30.06733951722958	20.295327337470646
14	20.44357574470317	28.1242878874	29.232711303425106	22.19942506447172
15	19.725981221295143	26.162060274500327	33.597526212193	20.514432292011524
16	22.788119145628265	24.486354525231036	31.24034214081363	21.48518418832707
17	22.084369166051115	25.28822018500094	28.674887975282758	23.952522673665182
18	23.053917188990063	26.177882610307044	28.429297806456717	22.338902394246173
19	24.47216601757827	26.502068512433862	27.050862790251724	21.974902679736143
20	26.723151258950583	25.708371993433733	28.120332303448325	19.44814444416736
21	24.688175297722186	26.810002123976602	27.518051651327312	20.983770926973904
22	23.579751881697085	24.966700002493738	27.109594612838624	24.343953502970557
23	23.31894131375262	26.381423201907623	28.106573750572917	22.193061733766843
24	23.085217896781614	25.109358997620628	28.839474664054837	22.96594844154292
25	22.625424257876556	27.03538441826689	27.202120880925744	23.137070442930813
26	22.124698924167156	27.361978067146897	27.770263123724646	22.7430598849613
27	23.701085119867095	27.254231399941354	27.09660997856245	21.948073501629096
28	21.071739674421046	27.855996106329535	28.638169837296513	22.434094381952907
29	22.922780981896324	25.38616388328276	28.54942717125013	23.14162796357079
30	23.628508753449314	26.360957354505455	26.99333484104142	23.017199051003814
31	23.630486545425153	26.793663842437056	26.216578540269136	23.359271071868662
32	24.378693848980966	26.56793758432488	25.62736851337976	23.426000053314393
33	23.534176675297292	28.780140904779632	25.399922436158164	22.28575998376491
34	22.094946053574084	25.927906902751968	28.856844837060038	23.120302206613907
35	22.433406454309136	25.360538578552305	27.17099215504513	25.03506281209342
36	23.51620456560379	28.8141073321908	26.502240494344804	21.167447607860606
37	24.11986107301234	27.42303164553152	26.915255053473473	21.54185222798266
38	22.593607604352176	26.925058022397202	25.630722160643142	24.85061221260748
39	23.076360828368074	26.77422988650054	26.920672483668167	23.228736801463224
40	22.479325624530812	25.808981411335157	29.15523345254546	22.55645951158857
41	20.378566582366865	26.812409870729798	28.049303774229028	24.75971977267431
42	22.452668428334707	26.19731656624356	27.543247001280406	23.806768004141325
43	23.755431403724955	26.32406723460826	26.811549961175086	23.108951400491694
44	22.58930805657861	25.838476309061814	26.804928657603792	24.767286976755784
45	21.512959266944303	24.87916120982395	27.28699395397592	26.320885569255825
46	22.73575065374624	26.287779051399372	27.53361601426762	23.442854280586765
47	21.29316638475965	25.994463902286757	29.37743408148331	23.334935631470284
48	22.261166570500116	24.976416980461995	28.223349468102942	24.539066980934944
49	21.67092465214509	25.294755497616762	29.33873815152122	23.69558169871693
50	21.14809964287956	25.294067569972988	28.54160199430224	25.016230792845207
51	20.858138141030327	24.897391292383865	26.972697011728307	27.271773554857496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	350.0
1	765.0
2	1180.0
3	3481.5
4	5783.0
5	4176.0
6	2569.0
7	2526.5
8	2484.0
9	2469.5
10	2455.0
11	2420.5
12	2386.0
13	2368.5
14	2351.0
15	2429.0
16	2507.0
17	2550.0
18	2593.0
19	2883.5
20	3174.0
21	3313.0
22	3452.0
23	3847.5
24	4243.0
25	5175.0
26	8213.0
27	10319.0
28	11822.0
29	13325.0
30	15836.0
31	18347.0
32	21307.0
33	24267.0
34	28620.5
35	32974.0
36	34243.5
37	35513.0
38	38666.5
39	41820.0
40	47922.5
41	54025.0
42	57460.5
43	60896.0
44	66925.0
45	72954.0
46	86176.0
47	99398.0
48	102475.5
49	105553.0
50	104094.5
51	102636.0
52	91150.0
53	79664.0
54	73369.5
55	67075.0
56	63923.5
57	60772.0
58	59069.0
59	57366.0
60	55367.0
61	53368.0
62	48616.5
63	43865.0
64	36667.5
65	29470.0
66	24678.0
67	19886.0
68	16809.5
69	13733.0
70	11920.5
71	10108.0
72	7968.0
73	5828.0
74	4617.5
75	2799.0
76	2191.0
77	1741.5
78	1292.0
79	894.0
80	496.0
81	422.0
82	348.0
83	240.0
84	132.0
85	178.0
86	224.0
87	121.5
88	19.0
89	11.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1162913.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.81314475239055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0111741006625	16.42801338548011
2	10.889027365258015	4.9682591499274595
3	4.4615412291953644	3.053453576411769
4	2.2044034619548025	2.0115750108098305
5	1.305883707521819	1.4895657024741853
6	0.8214489953277676	1.124390090227089
7	0.5787537237994492	0.9242234733915327
8	0.4524675338593463	0.8257765876552352
9	0.35686723199328535	0.7327137438762983
>10	4.6605683037683265	27.512691676206106
>50	2.0668817333965386	32.733817134348804
>100	0.18488336812867792	5.996452917149487
>500	0.0034308253879548308	0.5698773942552239
>1k	0.002668419746187091	1.6291901577868706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	3399	0.2922832576469607	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC	3056	0.2627883599203036	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2812	0.24180656678530552	No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	2653	0.22813400486536825	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG	2494	0.214461442945431	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC	2471	0.21248365096959101	No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT	1849	0.1589972766664402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7198191094260705E-4	0.0	0.0	0.16733839934715666	0.0
2	1.7198191094260705E-4	0.0	0.0	0.8764198181635255	0.0
3	1.7198191094260705E-4	0.0	0.0	1.216514047052531	0.0
4	1.7198191094260705E-4	0.0	0.0	1.6266909046506488	0.0
5	1.7198191094260705E-4	0.0	0.0	2.909331996460612	0.0
6	1.7198191094260705E-4	0.0	0.0	3.4723147819312365	0.0
7	1.7198191094260705E-4	0.0	0.0	4.086634167818229	0.0
8	1.7198191094260705E-4	0.0	0.0	4.992978838485768	0.0
9	1.7198191094260705E-4	0.0	0.0	5.295838983655699	0.0
10	1.7198191094260705E-4	0.0	0.0	6.209836849360184	0.0
11	1.7198191094260705E-4	0.0	0.0	7.2757807333824625	0.0
12	1.7198191094260705E-4	0.0	0.0	8.40174630432371	0.0
13	1.7198191094260705E-4	0.0	0.0	8.74080864174706	0.0
14	1.7198191094260705E-4	0.0	0.0	8.878394170501148	0.0
15	1.7198191094260705E-4	0.0	0.0	9.18925147452991	0.0
16	1.7198191094260705E-4	0.0	0.0	9.793251945760344	0.0
17	1.7198191094260705E-4	0.0	0.0	10.511018451079316	0.0
18	1.7198191094260705E-4	0.0	0.0	11.251400577687239	0.0
19	1.7198191094260705E-4	0.0	0.0	11.745418616869877	0.0
20	1.7198191094260705E-4	0.0	0.0	12.215617161386966	0.0
21	1.7198191094260705E-4	0.0	0.0	12.790896653489986	0.0
22	1.7198191094260705E-4	0.0	0.0	13.42387607671425	0.0
23	1.7198191094260705E-4	0.0	0.0	13.993394174800695	0.0
24	4.2995477735651767E-4	0.0	0.0	14.451811958418213	0.0
25	4.2995477735651767E-4	0.0	0.0	14.868094173854795	0.0
26	4.2995477735651767E-4	0.0	0.0	15.256257346852259	0.0
27	4.2995477735651767E-4	0.0	0.0	15.643990565072366	0.0
28	6.019366882991247E-4	0.0	0.0	16.045568327123352	0.0
29	6.019366882991247E-4	0.0	0.0	16.461420587782577	0.0
30	6.019366882991247E-4	0.0	0.0	16.91347504069522	0.0
31	6.879276437704282E-4	0.0	0.0	17.359424135769398	0.0
32	6.879276437704282E-4	0.0	0.0	17.78688517541725	0.0
33	7.739185992417317E-4	0.0	0.0	18.194912259128586	0.0
34	7.739185992417317E-4	0.0	0.0	18.603455288572746	0.0
35	7.739185992417317E-4	0.0	0.0	19.045104835873364	0.0
36	7.739185992417317E-4	0.0	0.0	19.481336952979287	0.0
37	7.739185992417317E-4	0.0	0.0	19.917483079129738	0.0
38	7.739185992417317E-4	0.0	0.0	20.35827271687564	0.0
39	8.599095547130353E-4	0.0	0.0	20.810413160743753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	30	2.1655233E-6	45.000004	31
ATCGGGT	30	2.1655233E-6	45.000004	4
ATCGCGT	30	2.1655233E-6	45.000004	26
GCGTACC	30	2.1655233E-6	45.000004	29
GCGAATA	20	7.033323E-4	45.0	9
CGCGCAT	25	3.8908343E-5	45.0	36
TAGCCGT	20	7.033323E-4	45.0	44
TCGTATC	25	3.8908343E-5	45.0	11
CGTCCCG	25	3.8908343E-5	45.0	11
AAAATCG	20	7.033323E-4	45.0	36
AATCGGC	20	7.033323E-4	45.0	3
ACAATCG	60	3.6379788E-12	41.250004	26
AGTACGG	120	0.0	41.250004	2
TTTAGCG	45	1.9283107E-8	40.000004	1
CGCAGTA	80	0.0	39.375	37
GATTCGA	40	3.4582627E-7	39.375	11
GCGTAAG	75	0.0	39.000004	1
CGGGTAC	110	0.0	38.863632	6
AAATCGG	35	6.248354E-6	38.571426	2
ACACGAC	140	0.0	38.571426	26
>>END_MODULE