##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934858.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1159627
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27211249824297	33.0	31.0	34.0	30.0	34.0
2	32.44668501164599	34.0	31.0	34.0	31.0	34.0
3	32.452160048015436	34.0	31.0	34.0	30.0	34.0
4	35.9797141667105	37.0	35.0	37.0	35.0	37.0
5	35.88547093160128	37.0	35.0	37.0	35.0	37.0
6	35.80266671955724	37.0	35.0	37.0	35.0	37.0
7	36.069201562226475	37.0	35.0	37.0	35.0	37.0
8	35.85843896356328	37.0	35.0	37.0	35.0	37.0
9	37.5365009610849	39.0	38.0	39.0	35.0	39.0
10	37.344563381156185	39.0	37.0	39.0	34.0	39.0
11	37.24033590111303	39.0	37.0	39.0	34.0	39.0
12	37.1425285889342	39.0	37.0	39.0	34.0	39.0
13	37.04161251850811	39.0	37.0	39.0	33.0	39.0
14	38.21954990699596	40.0	37.0	41.0	33.0	41.0
15	38.285706524598	40.0	37.0	41.0	34.0	41.0
16	38.3959919870786	40.0	37.0	41.0	34.0	41.0
17	38.37754467600358	40.0	37.0	41.0	34.0	41.0
18	38.338407953591975	40.0	37.0	41.0	34.0	41.0
19	38.32625318313561	40.0	37.0	41.0	34.0	41.0
20	38.24597392092457	40.0	37.0	41.0	34.0	41.0
21	38.190125790448135	40.0	37.0	41.0	34.0	41.0
22	38.11431089479635	40.0	37.0	41.0	34.0	41.0
23	37.91528827804113	40.0	36.0	41.0	33.0	41.0
24	37.64413643352561	40.0	36.0	41.0	33.0	41.0
25	37.571966675491346	39.0	36.0	41.0	33.0	41.0
26	37.68482882858022	40.0	36.0	41.0	33.0	41.0
27	37.75292400056225	40.0	36.0	41.0	33.0	41.0
28	37.68375434514719	40.0	36.0	41.0	33.0	41.0
29	37.705089653828345	40.0	36.0	41.0	33.0	41.0
30	37.61707600806121	40.0	36.0	41.0	33.0	41.0
31	37.50149918896335	40.0	36.0	41.0	33.0	41.0
32	37.44401432529598	40.0	36.0	41.0	33.0	41.0
33	37.307008201775226	40.0	35.0	41.0	33.0	41.0
34	37.25500182386233	40.0	35.0	41.0	32.0	41.0
35	37.225469051686446	40.0	35.0	41.0	32.0	41.0
36	37.14766299853315	39.0	35.0	41.0	32.0	41.0
37	37.092294332574184	39.0	35.0	41.0	32.0	41.0
38	37.00828542281268	39.0	35.0	41.0	32.0	41.0
39	37.01473577279591	39.0	35.0	41.0	32.0	41.0
40	36.92093578366147	39.0	35.0	41.0	31.0	41.0
41	36.79521691026511	39.0	35.0	41.0	31.0	41.0
42	36.71329746547812	39.0	35.0	41.0	31.0	41.0
43	36.653756768340166	39.0	35.0	41.0	31.0	41.0
44	36.608680204927964	39.0	35.0	41.0	31.0	41.0
45	36.569986728491145	39.0	35.0	41.0	31.0	41.0
46	36.513655684112216	39.0	35.0	41.0	31.0	41.0
47	36.39042295496741	39.0	35.0	41.0	31.0	41.0
48	36.34201687266681	39.0	35.0	40.0	31.0	41.0
49	36.311923575425546	39.0	35.0	40.0	31.0	41.0
50	36.21777433605806	38.0	35.0	40.0	31.0	41.0
51	35.26554831855415	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	16.0
10	24.0
11	24.0
12	18.0
13	26.0
14	28.0
15	46.0
16	79.0
17	124.0
18	260.0
19	472.0
20	887.0
21	1401.0
22	2206.0
23	3072.0
24	4386.0
25	6062.0
26	8245.0
27	9875.0
28	11379.0
29	13431.0
30	16232.0
31	20611.0
32	26998.0
33	38155.0
34	70565.0
35	94032.0
36	88050.0
37	135548.0
38	241577.0
39	365510.0
40	284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70409968032824	21.594616199864266	24.265647488373418	12.435636631434074
2	32.449399677654974	25.021149041890194	24.372578423924242	18.156872856530594
3	31.96933151780702	24.85195670676864	26.113827980893856	17.064883794530484
4	28.49976759768443	25.014422741105548	27.213750628434834	19.272059032775193
5	26.188679635779437	29.14290543424739	24.17535983553332	20.49305509443985
6	25.595040474221452	35.17312032231054	23.98616106730871	15.245678136159299
7	79.35310233376767	5.6215489980830045	9.584461210371956	5.440887457777372
8	79.90069220533844	5.605423123124935	8.576637142805401	5.917247528731221
9	73.69654207775432	6.687150264697182	10.910491045827667	8.705816611720838
10	40.69015295435515	25.770355467749546	18.042439508566115	15.49705206932919
11	30.239033758268825	25.45137358823139	24.637663662539765	19.671928990960026
12	30.209455281741455	20.602486834128563	28.84505103796307	20.343006846166915
13	25.213193552754465	23.24178378047424	30.212301024381116	21.332721642390183
14	19.551286749963566	27.773413347567793	28.541505156399428	24.13379474606921
15	17.849963824574626	26.14797689256977	34.80291507527852	21.199144207577092
16	20.152859497062416	24.61955439119648	32.44215596911766	22.785430142623447
17	20.385520516510912	24.789609072572475	29.231727098454936	25.59314331246168
18	20.595415594842134	26.270774999202327	30.290170891157242	22.843638514798293
19	23.49238160201513	27.15623213326354	26.730664256696336	22.620722008024995
20	25.758713793314573	25.96990239102746	27.915355541048974	20.35602827460899
21	24.547893417452336	26.19980390246174	27.67312247817617	21.579180201909754
22	22.344857441228946	25.35711914262086	26.575010757769522	25.723012658380668
23	22.514394714852276	26.078471784461726	28.078166513887652	23.328966986798342
24	22.68531174248271	24.052906667402535	29.212151838479095	24.049629751635656
25	21.45457116814286	26.82586728318675	26.532065914298304	25.187495634372087
26	20.585584847541494	27.155197317758212	28.40361599031413	23.855601844386168
27	22.369606778731438	27.00092357283851	27.069048926939438	23.56042072149062
28	19.241359506117053	27.840676355414285	28.8490178307335	24.06894630773516
29	20.890165544610465	24.452517921711035	29.101426579408724	25.555889954269777
30	21.289776798918965	25.16369487774948	27.027570072100772	26.518958251230785
31	20.81315802408878	28.049019210487508	25.744398845490835	25.39342391993287
32	21.796491458029177	27.29067191433107	25.332111101242038	25.580725526397714
33	21.075742458566417	25.961020224606706	25.957398370338048	27.00583894648883
34	20.410528557889734	25.32107306918518	29.578648996617012	24.68974937630807
35	20.764349226087354	22.363656589575786	27.701752373823652	29.170241810513208
36	20.30920287299278	26.31311620029544	27.944847782950898	25.43283314376088
37	20.367756183669403	26.803360045945805	28.446388364534457	24.382495405850328
38	19.924510208886133	26.452902528140516	26.158497516873965	27.46408974609939
39	21.985172818501123	23.081732315649774	28.494593520157775	26.43850134569133
40	21.845731429157826	22.552337950047733	31.00039926631581	24.601531354478638
41	18.850716652854754	24.616967352433154	30.424093264472113	26.108222730239984
42	20.646638962355997	24.498739680949132	28.84280893770152	26.011812418993348
43	22.02958365060489	24.55979379576364	28.278575783420013	25.13204677021146
44	21.29072537979885	25.11566219137705	27.08396751714129	26.509644911682805
45	20.37543106533394	23.733148676255382	27.00014746120951	28.891272797201168
46	21.380323155635388	24.549273171459443	27.860510319266453	26.209893353638712
47	19.269471994011866	24.387410779500648	31.807469125848225	24.535648100639257
48	19.871217210361607	22.70816391822543	29.97610438528941	27.444514486123555
49	19.985305619824306	22.71471774975919	31.098965443198544	26.201011187217958
50	19.874666595379374	22.436869786577926	30.17591001244366	27.512553605599045
51	19.081825449045255	22.38909580408183	28.094464858096615	30.43461388877631
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	316.0
1	789.5
2	1263.0
3	3659.0
4	6055.0
5	4265.0
6	2475.0
7	2377.5
8	2280.0
9	2295.0
10	2310.0
11	2292.0
12	2274.0
13	2296.0
14	2318.0
15	2309.0
16	2300.0
17	2216.0
18	2132.0
19	2352.0
20	2572.0
21	2781.5
22	2991.0
23	3427.5
24	3864.0
25	4394.5
26	7127.5
27	9330.0
28	11106.5
29	12883.0
30	15004.5
31	17126.0
32	19223.0
33	21320.0
34	24734.5
35	28149.0
36	29331.5
37	30514.0
38	33559.5
39	36605.0
40	42641.0
41	48677.0
42	53979.5
43	59282.0
44	66216.0
45	73150.0
46	90409.5
47	107669.0
48	114832.0
49	121995.0
50	119632.5
51	117270.0
52	100013.5
53	82757.0
54	73644.5
55	64532.0
56	58775.0
57	53018.0
58	52817.5
59	52617.0
60	52110.0
61	51603.0
62	48838.0
63	46073.0
64	38398.5
65	30724.0
66	24816.0
67	18908.0
68	15795.5
69	12683.0
70	10999.5
71	9316.0
72	7730.5
73	6145.0
74	5208.0
75	3275.5
76	2280.0
77	1785.5
78	1291.0
79	1112.0
80	933.0
81	616.5
82	300.0
83	178.5
84	57.0
85	51.5
86	46.0
87	32.0
88	18.0
89	12.0
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1159627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.475872960127663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60998568677854	16.34155180739118
2	12.104533239571788	5.683289691476596
3	5.052774288733779	3.558548618955408
4	2.6974512610334846	2.53300092480633
5	1.56818167101755	1.8407216843604357
6	1.040793009014449	1.4660114674447327
7	0.7408727480322067	1.217484415869733
8	0.5170284646573433	0.9710155642452516
9	0.38318373204334666	0.8096015352453485
>10	4.177588773391076	24.48487300807053
>50	1.9075907948637751	31.061815488808374
>100	0.1910936692628222	6.502038622557203
>500	0.005204885933228387	0.8302758921901888
>1k	0.0033459980999325347	2.208919642115157
>5k	3.7177756665917054E-4	0.4908516364635364
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	5624	0.48498353349827145	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	4281	0.36917043152668916	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC	3697	0.31880941026726695	No Hit
CTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGCT	3374	0.2909556262487851	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3349	0.28879976061267976	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCG	3316	0.2859540179730206	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC	3230	0.27853784018481803	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	1617	0.13944138934329744	No Hit
ACTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC	1279	0.11029408594315242	No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTG	1166	0.100549573267956	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.623462544421611E-5	0.0	0.0	0.30837502058851685	0.0
2	8.623462544421611E-5	0.0	0.0	1.4883234005417258	0.0
3	8.623462544421611E-5	0.0	0.0	2.078944350209162	0.0
4	8.623462544421611E-5	0.0	0.0	2.727601202800556	0.0
5	8.623462544421611E-5	0.0	0.0	4.626228951205862	0.0
6	8.623462544421611E-5	0.0	0.0	5.480469150856266	0.0
7	8.623462544421611E-5	0.0	0.0	6.399644023466166	0.0
8	8.623462544421611E-5	0.0	0.0	7.7272260821798735	0.0
9	8.623462544421611E-5	0.0	0.0	8.173317799602803	0.0
10	8.623462544421611E-5	0.0	0.0	9.546431740551057	0.0
11	8.623462544421611E-5	0.0	0.0	11.191443455524922	0.0
12	8.623462544421611E-5	0.0	0.0	12.768157347146971	0.0
13	8.623462544421611E-5	0.0	0.0	13.293326216102246	0.0
14	8.623462544421611E-5	0.0	0.0	13.501065428797363	0.0
15	8.623462544421611E-5	0.0	0.0	13.949830419609064	0.0
16	8.623462544421611E-5	0.0	0.0	14.773457327226772	0.0
17	8.623462544421611E-5	0.0	0.0	15.698151215865101	0.0
18	8.623462544421611E-5	0.0	0.0	16.68872835834281	0.0
19	8.623462544421611E-5	0.0	0.0	17.35402849364494	0.0
20	8.623462544421611E-5	0.0	0.0	17.94025147741472	0.0
21	8.623462544421611E-5	0.0	0.0	18.710412917257013	0.0
22	8.623462544421611E-5	0.0	0.0	19.550251934458235	0.0
23	8.623462544421611E-5	0.0	0.0	20.352320185714888	0.0
24	8.623462544421611E-5	0.0	0.0	20.980194493574228	0.0
25	8.623462544421611E-5	0.0	0.0	21.542616720721405	0.0
26	8.623462544421611E-5	0.0	0.0	22.019580434053363	0.0
27	1.7246925088843223E-4	0.0	0.0	22.494733220250996	0.0
28	1.7246925088843223E-4	0.0	0.0	23.012658380668956	0.0
29	4.311731272210806E-4	0.0	0.0	23.519373039779172	0.0
30	4.311731272210806E-4	0.0	0.0	24.07325803900737	0.0
31	4.311731272210806E-4	0.0	0.0	24.60955117464495	0.0
32	4.311731272210806E-4	0.0	0.0	25.12212978828537	0.0
33	4.311731272210806E-4	0.0	0.0	25.60564733315109	0.0
34	4.311731272210806E-4	0.0	0.0	26.088733704889588	0.0
35	4.311731272210806E-4	0.0	0.0	26.581564589303284	0.0
36	5.174077526652967E-4	0.0	0.0	27.087761840660832	0.0
37	5.174077526652967E-4	0.0	0.0	27.59982304654859	0.0
38	5.174077526652967E-4	0.0	0.0	28.076528056004214	0.0
39	5.174077526652967E-4	0.0	0.0	28.5441784297882	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.8908263E-5	45.000004	15
CTTCGAT	30	2.1655178E-6	45.000004	29
TCGTTTA	30	2.1655178E-6	45.000004	38
TCGTTAC	30	2.1655178E-6	45.000004	42
TCGATAA	25	3.8908263E-5	45.000004	42
TACGACT	30	2.1655178E-6	45.000004	24
AACGCAT	25	3.8908263E-5	45.000004	45
GAACGAC	30	2.1655178E-6	45.000004	44
ACGTAAG	25	3.8908263E-5	45.000004	1
GATTACG	30	2.1655178E-6	45.000004	1
CTCCGTA	20	7.033314E-4	45.0	31
CGTCTTA	20	7.033314E-4	45.0	40
ATTCGAC	20	7.033314E-4	45.0	10
CGTTAGA	20	7.033314E-4	45.0	11
CGAATTA	20	7.033314E-4	45.0	30
GGACGAT	40	6.8157533E-9	45.0	8
TACGTCC	20	7.033314E-4	45.0	45
ACGAAAC	20	7.033314E-4	45.0	24
CGTTTTT	2060	0.0	41.286407	1
AGTAACG	60	3.6379788E-12	41.250004	1
>>END_MODULE