SRR2934856.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934856.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	918019
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	4616	0.5028218370208024	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	3640	0.39650595466978356	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3616	0.39389162969393876	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	3395	0.36981805387470196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2947	0.32101732099226704	No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	2447	0.2665522173288353	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	2406	0.2620860788284338	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1559	0.16982219322258038	TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1242	0.1352913174999646	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG	1002	0.10914806774151734	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.032543E-4	45.000004	24
AACGTCA	20	7.032543E-4	45.000004	44
GTCGCGA	20	7.032543E-4	45.000004	21
TCGTTAA	20	7.032543E-4	45.000004	20
TCGCACT	20	7.032543E-4	45.000004	45
TCGCAAT	25	3.890189E-5	45.000004	42
CGTATTG	20	7.032543E-4	45.000004	12
GGTCGTA	20	7.032543E-4	45.000004	32
GCGAATC	20	7.032543E-4	45.000004	45
TTAGCGT	20	7.032543E-4	45.000004	16
CGTGCCG	20	7.032543E-4	45.000004	31
TAGCGTC	20	7.032543E-4	45.000004	17
TAGAACG	20	7.032543E-4	45.000004	1
CCAATAG	20	7.032543E-4	45.000004	43
CACGCAT	20	7.032543E-4	45.000004	37
GCGCCTA	20	7.032543E-4	45.000004	28
TTCGTTC	25	3.890189E-5	45.000004	18
ATGCTCG	20	7.032543E-4	45.000004	1
CGCGAAT	20	7.032543E-4	45.000004	44
CATTCGT	20	7.032543E-4	45.000004	22