##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934856.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	918019
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.286938505630054	33.0	31.0	34.0	30.0	34.0
2	32.46043273614163	34.0	31.0	34.0	31.0	34.0
3	32.469747358170146	34.0	31.0	34.0	30.0	34.0
4	35.988731170052034	37.0	35.0	37.0	35.0	37.0
5	35.89294230293709	37.0	35.0	37.0	35.0	37.0
6	35.826223640251456	37.0	35.0	37.0	35.0	37.0
7	36.112663245531955	37.0	35.0	37.0	35.0	37.0
8	35.885459886995804	37.0	35.0	37.0	35.0	37.0
9	37.59718589702392	39.0	38.0	39.0	35.0	39.0
10	37.393956987818335	39.0	37.0	39.0	34.0	39.0
11	37.280139082088716	39.0	37.0	39.0	34.0	39.0
12	37.099319295134414	39.0	37.0	39.0	34.0	39.0
13	36.994822547245754	39.0	37.0	39.0	33.0	39.0
14	38.14673007857136	40.0	37.0	41.0	33.0	41.0
15	38.22828830340113	40.0	37.0	41.0	33.0	41.0
16	38.329507341351324	40.0	37.0	41.0	34.0	41.0
17	38.31584858265461	40.0	37.0	41.0	34.0	41.0
18	38.278525825718205	40.0	37.0	41.0	34.0	41.0
19	38.256234348090835	40.0	37.0	41.0	34.0	41.0
20	38.16557282583476	40.0	36.0	41.0	34.0	41.0
21	38.10916331797054	40.0	36.0	41.0	34.0	41.0
22	38.05873734639479	40.0	36.0	41.0	34.0	41.0
23	37.886606922078954	40.0	36.0	41.0	34.0	41.0
24	37.630757097619984	39.0	35.0	41.0	33.0	41.0
25	37.60160955274346	39.0	35.0	41.0	33.0	41.0
26	37.745493284997366	40.0	35.0	41.0	33.0	41.0
27	37.809068221899544	40.0	36.0	41.0	34.0	41.0
28	37.77029342529948	40.0	36.0	41.0	34.0	41.0
29	37.79219711138876	40.0	36.0	41.0	34.0	41.0
30	37.695608696552036	40.0	36.0	41.0	33.0	41.0
31	37.60200932660435	40.0	35.0	41.0	33.0	41.0
32	37.43311739735234	40.0	35.0	41.0	33.0	41.0
33	37.30948596924465	40.0	35.0	41.0	33.0	41.0
34	37.23514654925443	40.0	35.0	41.0	33.0	41.0
35	37.08752324298299	39.0	35.0	41.0	32.0	41.0
36	37.0343358906515	39.0	35.0	41.0	32.0	41.0
37	36.99878433888623	39.0	35.0	41.0	32.0	41.0
38	36.89254579698242	39.0	35.0	41.0	31.0	41.0
39	36.933190925242286	39.0	35.0	41.0	32.0	41.0
40	36.793263538118495	39.0	35.0	41.0	31.0	41.0
41	36.69560760724996	39.0	35.0	41.0	31.0	41.0
42	36.601878610355556	39.0	35.0	41.0	31.0	41.0
43	36.59022961398402	39.0	35.0	41.0	31.0	41.0
44	36.58837888976154	39.0	35.0	41.0	31.0	41.0
45	36.540630422681886	39.0	35.0	41.0	31.0	41.0
46	36.47441392825203	39.0	35.0	41.0	31.0	41.0
47	36.31715792374668	39.0	35.0	41.0	31.0	41.0
48	36.24308865067063	38.0	35.0	40.0	31.0	41.0
49	36.22895386696789	38.0	35.0	40.0	31.0	41.0
50	36.131478760243525	38.0	35.0	40.0	31.0	41.0
51	35.24935213759192	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	19.0
11	9.0
12	5.0
13	19.0
14	22.0
15	39.0
16	49.0
17	92.0
18	201.0
19	365.0
20	659.0
21	1097.0
22	1790.0
23	2561.0
24	3677.0
25	5229.0
26	6877.0
27	8196.0
28	9202.0
29	10228.0
30	12293.0
31	15796.0
32	20906.0
33	29806.0
34	58325.0
35	83249.0
36	65321.0
37	101781.0
38	188987.0
39	290974.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.149672283471254	20.822444851359286	24.23631754898319	12.791565316186265
2	31.563725805239322	25.132595294868626	25.66373898579441	17.63993991409764
3	30.869077873115913	25.15536170819994	26.99203393393819	16.98352648474596
4	28.443420016361316	24.468556751004066	28.36804031289113	18.71998291974349
5	26.37788542502933	29.27891470655836	24.823887087304293	19.519312781108017
6	24.87399498267465	34.69481568464269	24.676613446998374	15.754575885684282
7	79.70760953749323	5.4534818996120995	10.302183288145454	4.536725274749216
8	79.13060622928285	6.276558546173881	9.06822189954674	5.524613324996541
9	74.04302089608167	6.578513080883947	11.248133208571936	8.130332814462445
10	40.588266691647995	25.405356534015088	18.887953299441516	15.118423474895401
11	31.474403035231298	25.560799939870527	23.951682917238095	19.01311410766008
12	29.125432044434813	21.692797207900927	28.843302807458233	20.33846794020603
13	23.34722919678133	25.876588610911107	30.538038973049574	20.23814321925799
14	18.94405235621485	30.722566744261286	28.79482886519778	21.538552034326088
15	17.742116448570236	25.529645900575044	36.82178691290704	19.90645073794769
16	19.758087795568503	24.87334140143069	33.235695557499355	22.132875245501456
17	20.25208628579583	25.28313684139435	28.698316701506176	25.766460171303645
18	20.710464598227272	26.427121878741072	31.052843132876333	21.809570390155322
19	23.97826188782585	26.20893467346536	27.798553188986286	22.0142502497225
20	25.445442850311377	27.056411686468362	28.630017461512235	18.868128001708026
21	22.533956268878967	28.112925767331614	29.171073801304765	20.182044162484654
22	22.4132615991608	24.775957796080473	28.076216287462458	24.734564317296265
23	20.896844182963534	26.249565640798284	28.7154187440565	24.138171432181686
24	21.13039054747233	24.273571679888978	31.717317397570206	22.87872037506849
25	20.368423747220916	27.574919473344234	29.197325981270538	22.859330798164308
26	19.545020310037156	28.94406324923558	29.05473633987968	22.456180100847586
27	21.8301581993401	27.279282890659122	29.509193164847353	21.38136574515342
28	17.534168682783253	26.383985516639637	31.65457359814993	24.427272202427183
29	18.881090696379925	25.066365728813896	32.08038177859064	23.972161796215545
30	21.46437056313649	26.129524552324078	29.7092979557068	22.69680692883263
31	23.556266264641582	25.94717538525891	26.36949779906516	24.127060551034347
32	23.054642659901376	25.858070475665535	27.623611276019343	23.46367558841375
33	23.255945683041418	25.765479799437703	27.908790558801073	23.069783958719807
34	22.243330475730893	23.860835124327494	29.352333666296666	24.543500733644947
35	21.489642371236325	22.88318651356889	31.093038379379944	24.534132735814836
36	22.799963835171166	25.427469366102446	30.193819517896685	21.5787472808297
37	21.388228348215016	25.65197452340311	30.922889395535385	22.03690773284649
38	20.75774030820713	25.77778891286564	27.409236628000073	26.05523415092716
39	24.006256951108856	23.59036141953489	29.07935456673555	23.324027062620708
40	24.45058326679513	23.24712233624794	30.827357603709725	21.4749367932472
41	20.195660438400513	24.54404538468158	30.472898709068115	24.787395467849795
42	20.20448378519399	23.3673812851368	31.855331970253335	24.572802959415874
43	21.479185071332946	24.02771620195225	30.80110542374395	23.691993302970854
44	21.0775593969188	24.303854277525847	28.844936760568135	25.773649564987217
45	21.774821654018055	23.694389767532044	27.33854092344494	27.192247655004962
46	23.39450490676119	24.465506705198912	28.61302434916924	23.526964038870656
47	20.937366220089125	23.46367558841375	32.62873644227407	22.970221749223054
48	20.23988610257522	22.39005946500018	32.07754959320014	25.29250483922446
49	21.40816257615583	22.003139368575162	33.16075157485847	23.427946480410537
50	20.796846252637472	22.010655552880714	31.43235597520313	25.760142219278688
51	20.59031457954574	22.13396454757472	28.586009657752182	28.689711215127357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	576.0
1	739.0
2	902.0
3	3688.0
4	6474.0
5	4497.0
6	2520.0
7	2420.0
8	2320.0
9	2258.5
10	2197.0
11	2202.5
12	2208.0
13	2203.5
14	2199.0
15	2140.0
16	2081.0
17	2077.0
18	2073.0
19	2237.0
20	2401.0
21	2604.5
22	2808.0
23	3194.0
24	3580.0
25	4442.5
26	6643.0
27	7981.0
28	10149.5
29	12318.0
30	13538.0
31	14758.0
32	16894.0
33	19030.0
34	19613.5
35	20197.0
36	23583.5
37	26970.0
38	29713.0
39	32456.0
40	36630.0
41	40804.0
42	45462.0
43	50120.0
44	57892.0
45	65664.0
46	77484.0
47	89304.0
48	93250.0
49	97196.0
50	95083.5
51	92971.0
52	81103.5
53	69236.0
54	60456.0
55	51676.0
56	46550.5
57	41425.0
58	37755.0
59	34085.0
60	34026.0
61	33967.0
62	31491.0
63	29015.0
64	24214.0
65	19413.0
66	16503.0
67	13593.0
68	10241.0
69	6889.0
70	5703.0
71	4517.0
72	3626.0
73	2735.0
74	2229.5
75	1460.5
76	1197.0
77	971.5
78	746.0
79	513.0
80	280.0
81	169.5
82	59.0
83	44.0
84	29.0
85	21.0
86	13.0
87	8.5
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	918019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.16201680867042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.69044115162326	15.223187113972608
2	12.26226512074335	5.435130514365742
3	5.395037482491805	3.58694734111171
4	2.9676115612334937	2.630730292066454
5	1.845428630723819	2.0449210166651457
6	1.180155452591497	1.5692774986306082
7	0.8183805994314222	1.2695875220342265
8	0.5955627485140962	1.0559097314549883
9	0.44099085036584107	0.8795921974449868
>10	3.5013686850225505	17.69966577203608
>50	1.6493732127306613	26.844129935227013
>100	0.6449312803447973	18.303056301879288
>500	0.003480739369632676	0.4967829164416176
>1k	0.004972484813760966	2.961081846669535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	4616	0.5028218370208024	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	3640	0.39650595466978356	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3616	0.39389162969393876	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	3395	0.36981805387470196	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2947	0.32101732099226704	No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	2447	0.2665522173288353	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	2406	0.2620860788284338	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1559	0.16982219322258038	TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1242	0.1352913174999646	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTG	1002	0.10914806774151734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.267906219805908E-4	0.0	0.0	0.27940598179340514	0.0
2	3.267906219805908E-4	0.0	0.0	1.5535626168957288	0.0
3	3.267906219805908E-4	0.0	0.0	2.1463608051685203	0.0
4	3.267906219805908E-4	0.0	0.0	2.828046042620033	0.0
5	3.267906219805908E-4	0.0	0.0	4.7564375029275	0.0
6	3.267906219805908E-4	0.0	0.0	5.670361942399885	0.0
7	3.267906219805908E-4	0.0	0.0	6.619579769046174	0.0
8	3.267906219805908E-4	0.0	0.0	8.00756847080507	0.0
9	3.267906219805908E-4	0.0	0.0	8.470303991529587	0.0
10	3.267906219805908E-4	0.0	0.0	9.915481052135087	0.0
11	3.267906219805908E-4	0.0	0.0	11.647580278839545	0.0
12	3.267906219805908E-4	0.0	0.0	13.332185935149491	0.0
13	3.267906219805908E-4	0.0	0.0	13.896008688273337	0.0
14	3.267906219805908E-4	0.0	0.0	14.105045756133588	0.0
15	3.267906219805908E-4	0.0	0.0	14.544252352075501	0.0
16	3.267906219805908E-4	0.0	0.0	15.419833358568832	0.0
17	3.267906219805908E-4	0.0	0.0	16.40968215254804	0.0
18	3.267906219805908E-4	0.0	0.0	17.53493119423454	0.0
19	3.267906219805908E-4	0.0	0.0	18.240581077297964	0.0
20	4.357208293074544E-4	0.0	0.0	18.873683442281695	0.0
21	4.357208293074544E-4	0.0	0.0	19.637719916472317	0.0
22	4.357208293074544E-4	0.0	0.0	20.4316032674705	0.0
23	4.357208293074544E-4	0.0	0.0	21.22733843199324	0.0
24	4.357208293074544E-4	0.0	0.0	21.891812696687104	0.0
25	4.357208293074544E-4	0.0	0.0	22.43450298958954	0.0
26	4.357208293074544E-4	0.0	0.0	22.89636706865544	0.0
27	5.44651036634318E-4	0.0	0.0	23.354745381086882	0.0
28	6.535812439611816E-4	0.0	0.0	23.858765450388283	0.0
29	6.535812439611816E-4	0.0	0.0	24.385878723642975	0.0
30	6.535812439611816E-4	0.0	0.0	24.98619309622132	0.0
31	6.535812439611816E-4	0.0	0.0	25.53694422446594	0.0
32	6.535812439611816E-4	0.0	0.0	26.073534425758073	0.0
33	7.625114512880452E-4	0.0	0.0	26.54138966622695	0.0
34	7.625114512880452E-4	0.0	0.0	27.04192396889389	0.0
35	7.625114512880452E-4	0.0	0.0	27.557926361001243	0.0
36	7.625114512880452E-4	0.0	0.0	28.095932654988623	0.0
37	7.625114512880452E-4	0.0	0.0	28.596684818070216	0.0
38	7.625114512880452E-4	0.0	0.0	29.08654396041912	0.0
39	7.625114512880452E-4	0.0	0.0	29.57237268509693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.032543E-4	45.000004	24
AACGTCA	20	7.032543E-4	45.000004	44
GTCGCGA	20	7.032543E-4	45.000004	21
TCGTTAA	20	7.032543E-4	45.000004	20
TCGCACT	20	7.032543E-4	45.000004	45
TCGCAAT	25	3.890189E-5	45.000004	42
CGTATTG	20	7.032543E-4	45.000004	12
GGTCGTA	20	7.032543E-4	45.000004	32
GCGAATC	20	7.032543E-4	45.000004	45
TTAGCGT	20	7.032543E-4	45.000004	16
CGTGCCG	20	7.032543E-4	45.000004	31
TAGCGTC	20	7.032543E-4	45.000004	17
TAGAACG	20	7.032543E-4	45.000004	1
CCAATAG	20	7.032543E-4	45.000004	43
CACGCAT	20	7.032543E-4	45.000004	37
GCGCCTA	20	7.032543E-4	45.000004	28
TTCGTTC	25	3.890189E-5	45.000004	18
ATGCTCG	20	7.032543E-4	45.000004	1
CGCGAAT	20	7.032543E-4	45.000004	44
CATTCGT	20	7.032543E-4	45.000004	22
>>END_MODULE