##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1585187
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40656023548011	33.0	31.0	34.0	31.0	34.0
2	32.58077312014292	34.0	31.0	34.0	31.0	34.0
3	32.59663118609981	34.0	31.0	34.0	31.0	34.0
4	36.08908791202552	37.0	35.0	37.0	35.0	37.0
5	35.98340574329716	37.0	35.0	37.0	35.0	37.0
6	35.91373320624002	37.0	35.0	37.0	35.0	37.0
7	36.13329657636607	37.0	35.0	37.0	35.0	37.0
8	35.90169740226232	37.0	35.0	37.0	35.0	37.0
9	37.59728221339186	39.0	38.0	39.0	35.0	39.0
10	37.43029686718349	39.0	37.0	39.0	35.0	39.0
11	37.33848120127152	39.0	37.0	39.0	35.0	39.0
12	37.258869142883455	39.0	37.0	39.0	34.0	39.0
13	37.226942941116725	39.0	37.0	39.0	34.0	39.0
14	38.462448278972765	40.0	38.0	41.0	34.0	41.0
15	38.509372711232174	40.0	38.0	41.0	34.0	41.0
16	38.62972759680719	40.0	38.0	41.0	35.0	41.0
17	38.575556070040946	40.0	38.0	41.0	34.0	41.0
18	38.57156285031356	40.0	38.0	41.0	35.0	41.0
19	38.55165226563175	40.0	38.0	41.0	34.0	41.0
20	38.45831564351714	40.0	38.0	41.0	34.0	41.0
21	38.42987357327558	40.0	37.0	41.0	34.0	41.0
22	38.37477660364361	40.0	37.0	41.0	34.0	41.0
23	38.191770434655346	40.0	37.0	41.0	34.0	41.0
24	37.987517561019615	40.0	37.0	41.0	34.0	41.0
25	37.920290161350046	40.0	36.0	41.0	33.0	41.0
26	38.028449009485946	40.0	37.0	41.0	34.0	41.0
27	38.07903862446513	40.0	37.0	41.0	34.0	41.0
28	38.03125372590111	40.0	37.0	41.0	34.0	41.0
29	38.04567410658805	40.0	37.0	41.0	34.0	41.0
30	37.942160767152394	40.0	37.0	41.0	34.0	41.0
31	37.872416945130134	40.0	37.0	41.0	33.0	41.0
32	37.76970855804394	40.0	36.0	41.0	33.0	41.0
33	37.672623482276855	40.0	36.0	41.0	33.0	41.0
34	37.602692931496414	40.0	36.0	41.0	33.0	41.0
35	37.549355375738	40.0	36.0	41.0	33.0	41.0
36	37.478655830510846	40.0	36.0	41.0	33.0	41.0
37	37.40557170857445	40.0	36.0	41.0	33.0	41.0
38	37.32122834719185	40.0	36.0	41.0	33.0	41.0
39	37.338194168889856	40.0	36.0	41.0	33.0	41.0
40	37.269533499833145	40.0	36.0	41.0	33.0	41.0
41	37.196006527936454	40.0	35.0	41.0	32.0	41.0
42	37.139031546435845	40.0	35.0	41.0	32.0	41.0
43	37.08607060239581	40.0	35.0	41.0	32.0	41.0
44	37.054188559457025	39.0	35.0	41.0	32.0	41.0
45	37.01470930558981	39.0	35.0	41.0	32.0	41.0
46	36.96714772452714	39.0	35.0	41.0	32.0	41.0
47	36.82203235328072	39.0	35.0	41.0	31.0	41.0
48	36.769287787497625	39.0	35.0	41.0	31.0	41.0
49	36.738545673160324	39.0	35.0	41.0	31.0	41.0
50	36.64605248465954	39.0	35.0	41.0	31.0	41.0
51	35.751469069579805	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	21.0
10	28.0
11	42.0
12	21.0
13	26.0
14	38.0
15	57.0
16	105.0
17	132.0
18	336.0
19	581.0
20	1083.0
21	1894.0
22	3139.0
23	4443.0
24	6141.0
25	7894.0
26	9862.0
27	11770.0
28	13206.0
29	15311.0
30	18568.0
31	23756.0
32	31657.0
33	44896.0
34	83450.0
35	117835.0
36	107800.0
37	169313.0
38	321396.0
39	589908.0
40	468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.033597928824804	21.157693067127095	24.778275370666048	11.030433633382057
2	30.1779537682305	27.01851579655902	24.81215150010693	17.991378935103555
3	30.99552292568637	26.376067933940917	25.72333737281469	16.90507176755802
4	28.64482234588096	24.938950420360502	28.017956241124864	18.398270992633677
5	26.885219220192948	30.031977299839074	23.44682362396361	19.635979856004372
6	26.473532775628367	36.269664083795796	22.305444089561675	14.95135905101417
7	81.67604200640051	5.198566478276696	8.977742058192502	4.147649457130295
8	82.94314803237725	6.232324640562911	6.5810532132801995	4.243474113779636
9	77.10736966679642	7.08137273394243	8.090654288736912	7.720603310524248
10	39.98777431306212	32.13109872841501	15.095190662048076	12.785936296474802
11	29.456966275903095	24.268303991894964	27.05857416191276	19.216155570289185
12	27.646075825754313	22.294341298534494	29.42138687738418	20.63819599832701
13	24.87782198567109	23.074943208593055	31.168499363166617	20.878735442569234
14	19.498456648963185	28.87880104997076	29.10155079495353	22.52119150611253
15	18.295317839472567	27.425534022168996	33.791092155058045	20.488055983300395
16	20.753261287154135	25.50336332558872	32.05659647726104	21.686778909996107
17	21.24102708387086	26.194196646830942	29.33817902872027	23.226597240577927
18	21.5582767206645	27.029681671626122	28.693333972584938	22.718707635124435
19	23.237258443325615	27.648662271391323	27.441052695991075	21.67302658929199
20	26.663037231569525	25.950692252712145	28.26202839160301	19.124242124115327
21	24.59552090699709	27.278863629338368	27.654907591344113	20.470707872320425
22	22.632219416384313	24.932389680208075	28.28372930133795	24.15166160206966
23	22.437226648969492	27.172756274180905	28.702418074334446	21.68759900251516
24	22.53999055001082	25.545440380220125	28.876403856453532	23.038165213315526
25	21.951668793650214	28.262911568161993	27.41234945782422	22.373070180363577
26	20.69863050857722	28.37229929339567	28.918354743005082	22.010715455022027
27	22.489838738268734	28.081923457610998	28.437843610879977	20.99039419324029
28	19.620902770461782	28.71030357932534	30.21309157846992	21.455702071742955
29	21.82550071379591	25.974033347485186	29.81263409301237	22.387831845706533
30	23.317753678272656	26.705745126600206	28.414691768226714	21.56180942690042
31	22.53488074277672	28.3372876512361	25.773299932436995	23.354531673550184
32	23.989409451377032	29.09751341639819	25.86533954669071	21.047737585534072
33	23.927145503968934	28.48932018746053	25.419461552485608	22.16407275608493
34	21.861584784634243	27.078256382370032	27.81615039739791	23.24400843559782
35	22.441579447724465	25.712613086027076	27.36888455431441	24.47692291193405
36	22.284121684066296	29.71434915880587	26.41776648433276	21.58376267279507
37	22.880265861377868	29.135363840354483	26.87985707679914	21.10451322146851
38	21.60741918776775	28.495565507413325	25.774120024956048	24.12289527986288
39	22.72085249248196	27.282396335574287	26.792738017659744	23.204013154284006
40	23.11866044826257	24.598738193033377	28.96390141983249	23.318699938871564
41	19.493409925769008	27.68884680482492	27.837598970973144	24.98014429843293
42	20.753639791393695	27.118882504083114	28.14690001873596	23.98057768578723
43	22.43804674148854	28.136995824467398	26.81254640619687	22.612411027847187
44	22.340392647681316	27.010945711767757	27.013910661644335	23.63475097890659
45	21.84303807689566	25.481346995654143	26.415558542935315	26.260056384514886
46	22.36789728908955	27.184805325806987	27.01820037635938	23.42909700874408
47	20.991529705958982	26.590364417573447	29.605718442051316	22.812387434416255
48	21.214847207301094	26.145432683967258	27.738809364447224	24.90091074428443
49	20.531205466610565	26.380420732695892	29.142744673025962	23.945629127667587
50	20.248273547537295	25.845468074113654	29.116438628376333	24.789819749972715
51	20.359364541849008	25.500019871472578	26.50337152651391	27.637244060164512
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	812.0
2	1313.0
3	3963.0
4	6613.0
5	4709.5
6	2806.0
7	2715.5
8	2625.0
9	2553.5
10	2482.0
11	2473.5
12	2465.0
13	2567.0
14	2669.0
15	2639.0
16	2609.0
17	2456.0
18	2303.0
19	2964.5
20	3626.0
21	4146.5
22	4667.0
23	5361.0
24	6055.0
25	8347.0
26	13402.0
27	16165.0
28	19537.5
29	22910.0
30	26905.5
31	30901.0
32	35330.0
33	39759.0
34	44638.5
35	49518.0
36	54617.0
37	59716.0
38	61992.0
39	64268.0
40	72129.5
41	79991.0
42	86118.0
43	92245.0
44	98011.5
45	103778.0
46	127379.5
47	150981.0
48	146140.5
49	141300.0
50	137927.0
51	134554.0
52	121133.0
53	107712.0
54	96757.0
55	85802.0
56	79394.5
57	72987.0
58	70415.5
59	67844.0
60	66054.5
61	64265.0
62	61064.0
63	57863.0
64	47432.0
65	37001.0
66	29512.5
67	22024.0
68	17369.5
69	12715.0
70	10540.5
71	8366.0
72	6594.0
73	4822.0
74	3842.5
75	2306.5
76	1750.0
77	1411.0
78	1072.0
79	770.5
80	469.0
81	370.5
82	272.0
83	147.5
84	23.0
85	20.0
86	17.0
87	13.0
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	20.0
98	39.0
99	19.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1585187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.45989864745218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.73497379180797	15.394252670498378
2	11.328032286073727	4.861968494684164
3	4.432051224280293	2.853341102201144
4	2.2007529253096703	1.8891173890091768
5	1.2578090405989673	1.3496227264551452
6	0.8364057704169796	1.0769509836775522
7	0.577835399755913	0.8680202379570335
8	0.4401709550387088	0.7556819266146337
9	0.3553430467090176	0.6863063190707146
>10	4.27118060422453	23.560894204873296
>50	2.2259193930375956	34.15593933903097
>100	0.3324026488442991	9.268930661206538
>500	0.003264668871882144	0.544234647507789
>1k	0.0029678807926201306	1.3741208332335342
>5k	8.903642377860392E-4	1.3606184639799763
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	8148	0.5140087573264227	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	7122	0.4492845323611662	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	6093	0.3843710552761283	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	4804	0.30305572781003126	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3508	0.22129881206444413	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	2968	0.18723343050378283	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	2302	0.14521945991230056	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT	1737	0.1095769773534605	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.261680798543011E-4	0.0	0.0	0.1537358053024659	0.0
2	1.261680798543011E-4	0.0	0.0	0.822552796610116	0.0
3	1.261680798543011E-4	0.0	0.0	1.14724635011516	0.0
4	1.261680798543011E-4	0.0	0.0	1.5393767423023277	0.0
5	1.261680798543011E-4	0.0	0.0	2.9179522668303486	0.0
6	1.261680798543011E-4	0.0	0.0	3.458708657085883	0.0
7	1.261680798543011E-4	0.0	0.0	4.06362151594733	0.0
8	1.261680798543011E-4	0.0	0.0	4.934307435021862	0.0
9	1.261680798543011E-4	0.0	0.0	5.219447295492582	0.0
10	1.261680798543011E-4	0.0	0.0	6.352878240863697	0.0
11	1.261680798543011E-4	0.0	0.0	7.368468199650893	0.0
12	1.261680798543011E-4	0.0	0.0	8.715186284015703	0.0
13	1.261680798543011E-4	0.0	0.0	9.059814394137726	0.0
14	1.261680798543011E-4	0.0	0.0	9.203582921131703	0.0
15	1.261680798543011E-4	0.0	0.0	9.523734423761992	0.0
16	1.261680798543011E-4	0.0	0.0	10.068275856413155	0.0
17	1.261680798543011E-4	0.0	0.0	10.782891860707917	0.0
18	1.261680798543011E-4	0.0	0.0	11.472400417111672	0.0
19	1.261680798543011E-4	0.0	0.0	12.05933432459388	0.0
20	1.261680798543011E-4	0.0	0.0	12.492784762933333	0.0
21	1.261680798543011E-4	0.0	0.0	13.03278414470974	0.0
22	1.261680798543011E-4	0.0	0.0	13.681351159200776	0.0
23	1.8925211978145165E-4	0.0	0.0	14.2643738562075	0.0
24	1.8925211978145165E-4	0.0	0.0	14.747408349929692	0.0
25	1.8925211978145165E-4	0.0	0.0	15.143386868552417	0.0
26	1.8925211978145165E-4	0.0	0.0	15.517727561480129	0.0
27	1.8925211978145165E-4	0.0	0.0	15.90802851650941	0.0
28	1.8925211978145165E-4	0.0	0.0	16.28993929422838	0.0
29	1.8925211978145165E-4	0.0	0.0	16.712602361740288	0.0
30	2.523361597086022E-4	0.0	0.0	17.17854108064222	0.0
31	3.785042395629033E-4	0.0	0.0	17.635395697794646	0.0
32	3.785042395629033E-4	0.0	0.0	18.048217655077917	0.0
33	3.785042395629033E-4	0.0	0.0	18.450126073453795	0.0
34	3.785042395629033E-4	0.0	0.0	18.873609233484757	0.0
35	3.785042395629033E-4	0.0	0.0	19.309772285541076	0.0
36	3.785042395629033E-4	0.0	0.0	19.75823672538319	0.0
37	3.785042395629033E-4	0.0	0.0	20.192318004121912	0.0
38	3.785042395629033E-4	0.0	0.0	20.5968128681348	0.0
39	3.785042395629033E-4	0.0	0.0	21.03562544986806	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGT	20	7.0340966E-4	45.000004	23
CGCGAAT	20	7.0340966E-4	45.000004	19
TAGTTCG	20	7.0340966E-4	45.000004	28
CGTACGT	20	7.0340966E-4	45.000004	28
TGCGTAC	20	7.0340966E-4	45.000004	18
CTTACCG	20	7.0340966E-4	45.000004	1
AAATGCG	50	2.1827873E-11	45.0	1
TCCGCGT	30	2.1660253E-6	44.999996	43
ACCGTAA	30	2.1660253E-6	44.999996	17
GTATGCG	105	0.0	42.857143	1
GCTACGA	255	0.0	42.35294	10
ATTACGC	55	6.184564E-11	40.909092	12
TATACGG	105	0.0	40.714287	2
AATTGCG	50	1.0822987E-9	40.5	1
TATAGCG	90	0.0	40.0	1
CGAATAT	250	0.0	39.600002	14
TACGGCT	995	0.0	39.572865	7
TACGGGA	850	0.0	39.17647	4
CTACGAA	265	0.0	39.056602	11
TTAGGCG	145	0.0	38.793102	1
>>END_MODULE