##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934853.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1157624
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45256145345984	33.0	31.0	34.0	31.0	34.0
2	32.62415948529056	34.0	31.0	34.0	31.0	34.0
3	32.61980574003303	34.0	31.0	34.0	31.0	34.0
4	36.12386146106162	37.0	35.0	37.0	35.0	37.0
5	36.02562144530521	37.0	35.0	37.0	35.0	37.0
6	35.962887777032954	37.0	35.0	37.0	35.0	37.0
7	36.18955982253305	37.0	36.0	37.0	35.0	37.0
8	35.97955208254148	37.0	36.0	37.0	35.0	37.0
9	37.65532158973898	39.0	38.0	39.0	35.0	39.0
10	37.50429845960346	39.0	37.0	39.0	35.0	39.0
11	37.37567638542394	39.0	37.0	39.0	35.0	39.0
12	37.21633276435181	39.0	37.0	39.0	34.0	39.0
13	37.139505573485	39.0	37.0	39.0	33.0	39.0
14	38.36467540410358	40.0	38.0	41.0	34.0	41.0
15	38.430126707808405	40.0	38.0	41.0	34.0	41.0
16	38.52847729487295	40.0	38.0	41.0	35.0	41.0
17	38.51099061526022	40.0	38.0	41.0	34.0	41.0
18	38.47932230154178	40.0	38.0	41.0	34.0	41.0
19	38.458769859643546	40.0	37.0	41.0	34.0	41.0
20	38.40360427910962	40.0	37.0	41.0	34.0	41.0
21	38.345913699094005	40.0	37.0	41.0	34.0	41.0
22	38.28616718381789	40.0	37.0	41.0	34.0	41.0
23	38.137442727517744	40.0	36.0	41.0	34.0	41.0
24	37.92892424483252	40.0	36.0	41.0	34.0	41.0
25	37.85032704919732	40.0	36.0	41.0	34.0	41.0
26	37.96403495435478	40.0	36.0	41.0	34.0	41.0
27	38.01474399286815	40.0	36.0	41.0	34.0	41.0
28	37.97039453224881	40.0	36.0	41.0	34.0	41.0
29	37.96840165718748	40.0	36.0	41.0	34.0	41.0
30	37.853759078941	40.0	36.0	41.0	34.0	41.0
31	37.79102109147703	40.0	36.0	41.0	34.0	41.0
32	37.658814952005145	40.0	36.0	41.0	33.0	41.0
33	37.56461769970215	40.0	36.0	41.0	33.0	41.0
34	37.481424884072894	40.0	35.0	41.0	33.0	41.0
35	37.356150183479265	40.0	35.0	41.0	33.0	41.0
36	37.31754006482243	40.0	35.0	41.0	33.0	41.0
37	37.23670466403599	40.0	35.0	41.0	33.0	41.0
38	37.16039404849934	39.0	35.0	41.0	33.0	41.0
39	37.18750215959586	39.0	35.0	41.0	33.0	41.0
40	37.06867860376081	39.0	35.0	41.0	32.0	41.0
41	36.95281023890313	39.0	35.0	41.0	32.0	41.0
42	36.87703952233195	39.0	35.0	41.0	32.0	41.0
43	36.8626566138919	39.0	35.0	41.0	32.0	41.0
44	36.81689996060897	39.0	35.0	41.0	32.0	41.0
45	36.77770070420102	39.0	35.0	41.0	32.0	41.0
46	36.71279189097669	39.0	35.0	41.0	32.0	41.0
47	36.587606165732566	39.0	35.0	41.0	31.0	41.0
48	36.51494785871751	39.0	35.0	41.0	31.0	41.0
49	36.4623789762479	39.0	35.0	41.0	31.0	41.0
50	36.37207677104137	38.0	35.0	40.0	31.0	41.0
51	35.43066228758215	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	28.0
10	34.0
11	31.0
12	21.0
13	23.0
14	29.0
15	55.0
16	74.0
17	154.0
18	235.0
19	431.0
20	690.0
21	1158.0
22	1716.0
23	2561.0
24	3671.0
25	5256.0
26	7131.0
27	8640.0
28	9817.0
29	11149.0
30	13741.0
31	17969.0
32	23940.0
33	35121.0
34	71128.0
35	98255.0
36	82224.0
37	131161.0
38	238465.0
39	392380.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39606297035998	20.870075257596593	24.269797447184924	12.464064324858503
2	32.232227389895165	24.55598709079978	24.758989101815445	18.452796417489616
3	31.46781683862809	24.715538033074644	26.683448166243963	17.13319696205331
4	29.936749756397585	24.97658998085734	26.339294969696553	18.74736529304852
5	26.00585336862401	29.850625073426258	23.43731643435174	20.70620512359799
6	26.770263919891086	34.25525040945938	23.95181855248336	15.022667118166174
7	81.90137730385688	5.714981721180625	7.805556899304092	4.578084075658417
8	82.53612571957734	5.266304084918764	7.581218081173162	4.6163521143307324
9	76.73743806279069	7.190244846340435	9.289544791745852	6.782772299123032
10	42.55682328631749	26.968601203845115	16.360666330345605	14.113909179491788
11	32.54657816354879	25.098995874308066	23.519899380109603	18.834526582033543
12	30.736923215137214	22.383865572932145	27.48517653400413	19.394034677926513
13	24.86541398588834	24.608508462160426	29.187197224660167	21.338880327291072
14	20.685645770993	28.453625702300574	27.85723170908689	23.00349681761954
15	20.19351706599034	26.254293276573392	33.08544052300228	20.46674913443398
16	22.396995915772305	24.074051678265135	32.56584175863665	20.9631106473259
17	22.129033261231626	24.61058167418782	28.446369460204696	24.81401560437586
18	22.60068899746377	26.50161019467461	28.856087987118446	22.04161282074318
19	25.37922503334416	27.49917071518904	25.708952129534286	21.41265212193251
20	27.344543651479235	25.975273491219948	27.027428595122423	19.652754262178394
21	25.255091463203943	26.669194833555625	26.8181205641901	21.257593139050332
22	23.24424856430067	25.743592047158664	26.214297561211588	24.797861827329083
23	22.99079839395175	26.393716785415645	27.86872075907203	22.746764061560576
24	23.523959420329916	23.722555855787373	29.492304928024986	23.26117979585772
25	22.71756632550811	26.13171461545372	26.729663517687953	24.421055541350214
26	21.744020510977656	27.812312115160015	27.905174737220374	22.538492636641948
27	22.316486182041835	28.51228032590893	27.798490701644056	21.372742790405177
28	20.751211101359335	27.482671402804364	29.752406653628466	22.013710842207832
29	22.15598501758775	25.966635107772472	29.027646282385298	22.849733592254477
30	23.361730579186332	27.895326980090253	26.623152249780585	22.11979019094283
31	24.943159436915614	27.26334284707297	25.65323455629807	22.140263159713346
32	25.577216781960292	28.193005673690248	25.567196257161218	20.662581287188242
33	25.83438145719163	28.042179498697333	24.97676274852629	21.146676295584747
34	24.335621929054685	25.83135802298501	27.886515828973828	21.946504218986476
35	23.209695030510773	25.79810024671223	27.963829360828736	23.028375361948267
36	25.074894784489608	28.193005673690248	25.980974824295284	20.75112471752486
37	24.059711961742327	29.188838517515187	26.78633131310339	19.965118207639097
38	23.67400814081256	27.55126016737732	26.233733923968405	22.540997767841716
39	26.206868551446753	25.407731698720827	26.376958321527542	22.008441428304874
40	25.36393509464213	24.926660124530937	28.754414213941658	20.954990566885275
41	21.551039024761064	26.037297084372817	29.39849208378541	23.01317180708071
42	23.036495442388894	26.109082050821335	27.7668742182263	23.08754828856347
43	24.287419749417776	25.422676102084964	26.866927430668337	23.422976717828934
44	23.091953864121685	25.925689170231443	27.298155532366298	23.684201433280585
45	22.94406473950091	26.022698216346583	26.238571418698992	24.794665625453515
46	23.54944265149997	26.948560154246977	26.837297775443496	22.664699418809562
47	22.699944023275258	25.793435519650593	29.259241342612107	22.247379114462035
48	23.43057849526271	24.757434192794896	28.86023441117323	22.95175290076916
49	22.96298279925088	25.179332840369582	28.90714083329302	22.950543527086513
50	21.783843458670518	25.089148117177945	29.287748007988778	23.839260416162762
51	21.57064815518683	24.470207943166347	27.46496271673704	26.49418118490978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	883.5
2	1363.0
3	3494.0
4	5625.0
5	4002.0
6	2379.0
7	2267.0
8	2155.0
9	2196.0
10	2237.0
11	2249.0
12	2261.0
13	2243.0
14	2225.0
15	2099.0
16	1973.0
17	1987.5
18	2002.0
19	2376.0
20	2750.0
21	3011.0
22	3272.0
23	3876.0
24	4480.0
25	5430.0
26	7829.5
27	9279.0
28	11576.0
29	13873.0
30	16591.5
31	19310.0
32	21173.5
33	23037.0
34	27127.0
35	31217.0
36	33757.0
37	36297.0
38	37567.5
39	38838.0
40	46425.0
41	54012.0
42	58906.0
43	63800.0
44	69718.5
45	75637.0
46	83555.5
47	91474.0
48	97967.5
49	104461.0
50	102018.5
51	99576.0
52	90885.0
53	82194.0
54	74481.5
55	66769.0
56	63335.5
57	59902.0
58	59161.5
59	58421.0
60	56511.5
61	54602.0
62	50653.0
63	46704.0
64	39345.0
65	31986.0
66	25881.5
67	19777.0
68	16980.5
69	14184.0
70	11450.0
71	8716.0
72	7686.0
73	6656.0
74	5121.5
75	2792.0
76	1997.0
77	1471.0
78	945.0
79	668.5
80	392.0
81	372.5
82	353.0
83	221.5
84	90.0
85	53.5
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1157624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.568849872255065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.00380580765405	15.314704278240427
2	11.652594950729798	5.026661422289768
3	4.5732502167920766	2.9591924206273865
4	2.318105051061529	1.9999543933784911
5	1.3789699827767268	1.4871398268428686
6	0.9002519453189276	1.1650439433473718
7	0.6290720821889813	0.9497852909692727
8	0.47635751610754956	0.821958700035525
9	0.37290642377165284	0.7238846403657279
>10	4.101190049901383	22.246320137500167
>50	2.1453625815567	33.38872734084058
>100	0.4400223805182203	11.933711326873974
>500	0.005677708135718971	0.8565316600166382
>1k	0.0024333034867367017	1.1263846186717985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3227	0.27876063385002386	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2835	0.24489817073592118	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2797	0.24161558502588057	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	1954	0.16879401256366489	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7276766894950348E-4	0.0	0.0	0.17691409300429156	0.0
2	1.7276766894950348E-4	0.0	0.0	0.8941590706481551	0.0
3	1.7276766894950348E-4	0.0	0.0	1.2793445885710732	0.0
4	1.7276766894950348E-4	0.0	0.0	1.6819796410578909	0.0
5	1.7276766894950348E-4	0.0	0.0	2.7952081159340167	0.0
6	1.7276766894950348E-4	0.0	0.0	3.374152574583803	0.0
7	1.7276766894950348E-4	0.0	0.0	3.9368568723523354	0.0
8	1.7276766894950348E-4	0.0	0.0	4.817540064822429	0.0
9	1.7276766894950348E-4	0.0	0.0	5.13387766666897	0.0
10	1.7276766894950348E-4	0.0	0.0	5.962816942288688	0.0
11	1.7276766894950348E-4	0.0	0.0	7.091767275039218	0.0
12	1.7276766894950348E-4	0.0	0.0	8.036720040358528	0.0
13	1.7276766894950348E-4	0.0	0.0	8.403851336876222	0.0
14	1.7276766894950348E-4	0.0	0.0	8.549494481800654	0.0
15	1.7276766894950348E-4	0.0	0.0	8.85062852877964	0.0
16	1.7276766894950348E-4	0.0	0.0	9.449959572365465	0.0
17	1.7276766894950348E-4	0.0	0.0	10.163662812795865	0.0
18	1.7276766894950348E-4	0.0	0.0	10.923581404670255	0.0
19	1.7276766894950348E-4	0.0	0.0	11.377873990172976	0.0
20	1.7276766894950348E-4	0.0	0.0	11.84192794897134	0.0
21	1.7276766894950348E-4	0.0	0.0	12.422772851979571	0.0
22	1.7276766894950348E-4	0.0	0.0	13.043267935011714	0.0
23	1.7276766894950348E-4	0.0	0.0	13.67568398720137	0.0
24	1.7276766894950348E-4	0.0	0.0	14.154250430191496	0.0
25	1.7276766894950348E-4	0.0	0.0	14.582195946179416	0.0
26	1.7276766894950348E-4	0.0	0.0	14.995283442637678	0.0
27	1.7276766894950348E-4	0.0	0.0	15.381160031236394	0.0
28	1.7276766894950348E-4	0.0	0.0	15.782585710040566	0.0
29	1.7276766894950348E-4	0.0	0.0	16.19074932793377	0.0
30	1.7276766894950348E-4	0.0	0.0	16.658863326952446	0.0
31	1.7276766894950348E-4	0.0	0.0	17.10451752900769	0.0
32	1.7276766894950348E-4	0.0	0.0	17.55060365023531	0.0
33	1.7276766894950348E-4	0.0	0.0	17.978808317726653	0.0
34	1.7276766894950348E-4	0.0	0.0	18.382566360061645	0.0
35	1.7276766894950348E-4	0.0	0.0	18.839968763605455	0.0
36	2.591515034242552E-4	0.0	0.0	19.291065147232608	0.0
37	2.591515034242552E-4	0.0	0.0	19.71814682487578	0.0
38	2.591515034242552E-4	0.0	0.0	20.153866885966426	0.0
39	2.591515034242552E-4	0.0	0.0	20.581553250450924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	20	7.033306E-4	45.000004	6
CTCGTCT	40	6.8157533E-9	45.000004	17
GTCGACG	20	7.033306E-4	45.000004	2
CGGTGAT	20	7.033306E-4	45.000004	45
CATACGT	20	7.033306E-4	45.000004	44
ATACGTC	20	7.033306E-4	45.000004	45
ATACGTA	20	7.033306E-4	45.000004	43
CGCCGTA	20	7.033306E-4	45.000004	20
GCGTATA	20	7.033306E-4	45.000004	7
TACGTAA	20	7.033306E-4	45.000004	44
TCGTAAG	55	1.8189894E-12	45.0	1
GTATACG	45	3.8562575E-10	45.0	1
TCGCGCA	35	1.211938E-7	45.0	34
CGAACTA	50	2.1827873E-11	44.999996	36
CGACACG	25	3.890824E-5	44.999996	33
CGTTAGT	25	3.890824E-5	44.999996	23
CCGAACT	55	6.184564E-11	40.909092	35
ATCGCAA	55	6.184564E-11	40.909092	39
CCCGAAC	55	6.184564E-11	40.909092	34
CGAGGGT	105	0.0	40.714287	4
>>END_MODULE