##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934852.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593478
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12440393746693	33.0	31.0	34.0	30.0	34.0
2	32.3055125885037	34.0	31.0	34.0	30.0	34.0
3	32.298161010180664	34.0	31.0	34.0	30.0	34.0
4	35.86606748691611	37.0	35.0	37.0	35.0	37.0
5	35.76261125096465	37.0	35.0	37.0	35.0	37.0
6	35.69218404052046	37.0	35.0	37.0	33.0	37.0
7	36.01115626864012	37.0	35.0	37.0	35.0	37.0
8	35.8216698849831	37.0	35.0	37.0	35.0	37.0
9	37.49722483394498	39.0	37.0	39.0	35.0	39.0
10	37.30705603240558	39.0	37.0	39.0	34.0	39.0
11	37.172092310077204	39.0	37.0	39.0	34.0	39.0
12	36.92444370305218	39.0	35.0	39.0	33.0	39.0
13	36.65579179009163	39.0	35.0	39.0	33.0	39.0
14	37.72774053966617	40.0	36.0	41.0	33.0	41.0
15	37.820101166344834	40.0	36.0	41.0	33.0	41.0
16	37.97805310390613	40.0	36.0	41.0	33.0	41.0
17	37.99901260029858	40.0	36.0	41.0	34.0	41.0
18	37.97156929153229	40.0	36.0	41.0	34.0	41.0
19	37.93921762896013	40.0	36.0	41.0	34.0	41.0
20	37.86396631383135	40.0	35.0	41.0	34.0	41.0
21	37.77441960780349	39.0	35.0	41.0	34.0	41.0
22	37.68606923929784	39.0	35.0	41.0	33.0	41.0
23	37.556859731953	39.0	35.0	41.0	33.0	41.0
24	37.30962226063982	39.0	35.0	41.0	33.0	41.0
25	37.22892508231139	39.0	35.0	41.0	32.0	41.0
26	37.38701687341401	39.0	35.0	41.0	33.0	41.0
27	37.386993283660054	39.0	35.0	41.0	33.0	41.0
28	37.36714082072124	39.0	35.0	41.0	33.0	41.0
29	37.404426111835654	39.0	35.0	41.0	33.0	41.0
30	37.22637738888383	39.0	35.0	41.0	33.0	41.0
31	37.10266766417626	39.0	35.0	41.0	32.0	41.0
32	37.014187552023834	39.0	35.0	41.0	32.0	41.0
33	36.7902752924287	39.0	35.0	41.0	31.0	41.0
34	36.71681342863594	39.0	35.0	41.0	31.0	41.0
35	36.648057720757976	39.0	35.0	41.0	31.0	41.0
36	36.48159493696481	39.0	35.0	41.0	31.0	41.0
37	36.41464721522954	39.0	35.0	40.0	31.0	41.0
38	36.35889283174777	38.0	35.0	40.0	31.0	41.0
39	36.41449388182881	39.0	35.0	40.0	31.0	41.0
40	36.34281978438965	39.0	35.0	40.0	31.0	41.0
41	36.24123387893064	38.0	35.0	40.0	30.0	41.0
42	36.06418435055723	38.0	35.0	40.0	30.0	41.0
43	35.97613727888818	38.0	35.0	40.0	30.0	41.0
44	35.938941291842326	38.0	35.0	40.0	30.0	41.0
45	35.898703911518204	38.0	35.0	40.0	30.0	41.0
46	35.81314555889182	38.0	35.0	40.0	29.0	41.0
47	35.634412733075195	38.0	35.0	40.0	29.0	41.0
48	35.59712575697836	38.0	35.0	40.0	29.0	41.0
49	35.57825058384641	37.0	35.0	40.0	29.0	41.0
50	35.451091700113565	37.0	34.0	40.0	29.0	41.0
51	34.45540694010562	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	8.0
11	9.0
12	10.0
13	7.0
14	18.0
15	22.0
16	35.0
17	66.0
18	146.0
19	283.0
20	517.0
21	828.0
22	1162.0
23	1736.0
24	2656.0
25	3910.0
26	5189.0
27	6065.0
28	6856.0
29	7977.0
30	9700.0
31	12224.0
32	16153.0
33	23238.0
34	44057.0
35	63290.0
36	47180.0
37	71655.0
38	121194.0
39	147169.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.25086018352829	22.022046310056986	24.349512534584264	13.377580971830463
2	33.33367032981846	22.64447881808593	24.691226970502697	19.330623881592913
3	30.17382278702833	23.70719723393285	27.953015950043643	18.165964028995177
4	28.593309271784296	24.81995962782108	26.816326805711416	19.770404294683207
5	25.139263797478595	28.26456920054324	26.124136025261258	20.472030976716912
6	26.350429165023808	31.62543514671142	26.094648832812673	15.929486855452096
7	77.29283983568051	6.18017854073782	10.516312314862555	6.01066930871911
8	76.26651704022727	5.2970792514634075	11.178341909893879	7.258061798415443
9	70.63345229309257	6.769922389709475	13.831346739053513	8.765278578144429
10	41.730274753234326	22.80809061161492	20.230067500395972	15.231567134754783
11	32.10531814153178	22.76495506151871	24.470157276259606	20.6595695206899
12	29.136716104051036	21.20567232483765	29.93337579489046	19.724235776220855
13	23.160588935057408	28.51613707669029	30.830797434782752	17.492476553469547
14	18.193092246047872	30.741156369739066	29.75476765777333	21.310983726439733
15	15.375970128631558	24.835966960864596	40.49905809482407	19.28900481567977
16	18.62562723470794	23.855644185631146	35.68590579600255	21.83282278365837
17	18.136645334789158	23.492530472907166	28.99399809259989	29.376826099703777
18	20.422997988130984	24.25667000293187	33.96654972888633	21.35378228005082
19	23.07414933662242	25.23682427992276	28.460869653129517	23.228156730325303
20	24.701168366813935	26.10913968167312	28.245697397376148	20.9439945541368
21	22.405211313646	25.935754990075456	30.472233174607986	21.18680052167056
22	20.336389891453432	25.598421508463666	26.71185789532215	27.353330704760747
23	18.437583196007264	27.106480779405473	29.145309514421765	25.310626510165502
24	22.19677898759516	22.512207697673713	31.99141332955897	23.299599985172154
25	19.581180768284586	25.774670670184914	29.591324362486898	25.052824199043606
26	20.217261633961158	28.056305372734965	28.079052635481013	23.64738035782287
27	20.790492655161607	30.027734810725924	28.810166509963302	20.37160602414917
28	17.41783183201399	26.67327179777515	30.69498785127671	25.213908518934147
29	20.145312884386616	24.62096320335379	29.879456357270197	25.354267554989402
30	21.494309814348636	27.667748425383927	28.76568297392658	22.07225878634086
31	24.638655518822937	28.92440831842124	23.705849247992344	22.73108691476348
32	25.692949022541693	26.54521313342702	24.767556674383886	22.9942811696474
33	23.271292280421516	26.79475903066331	28.132298080131022	21.80165060878415
34	22.67396601053451	25.419307876618845	28.616562029258034	23.290164083588607
35	22.396617903275267	23.748479303360867	29.035448660270475	24.81945413309339
36	25.077256444215283	26.905799372512547	28.71648148709809	19.30046269617408
37	22.726874458699395	27.68527224261051	29.204957892289187	20.382895406400912
38	19.743781572358202	28.38403445452064	26.765271838214726	25.106912134906434
39	22.78635433832425	23.93837682272974	30.72110507887403	22.554163760071983
40	26.530722284566572	21.629276906641863	29.87962485551276	21.960375953278806
41	22.145892518341036	24.44842100296894	27.12956503863665	26.27612144005338
42	24.435615136534125	23.739885892990138	28.476034494960217	23.34846447551552
43	23.39951944301221	24.216398922959232	28.51681106966054	23.867270564368013
44	21.33811194349243	24.19146118305986	28.522371511665135	25.94805536178258
45	21.748068167649013	24.69190096347295	25.778377631521305	27.781653237356736
46	24.72560061198562	24.575300179619127	28.58437886492844	22.114720343466818
47	20.904902961862106	24.284977707682508	31.4191932978139	23.39092603264148
48	20.79672709013645	22.28439807372809	31.22154485928712	25.697329976848344
49	22.446493383074014	20.089371467855592	34.04304793101008	23.421087218060315
50	20.93371616134044	22.22104273452428	31.467215296944453	25.37802580719083
51	20.371437525906604	22.520969606287007	26.740671094800483	30.366921773005906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	410.0
1	489.0
2	568.0
3	2315.5
4	4063.0
5	2767.5
6	1472.0
7	1486.5
8	1501.0
9	1638.5
10	1776.0
11	1718.5
12	1661.0
13	1586.5
14	1512.0
15	1544.5
16	1577.0
17	1439.5
18	1302.0
19	1479.5
20	1657.0
21	1566.5
22	1476.0
23	1832.0
24	2188.0
25	2275.5
26	2926.0
27	3489.0
28	4123.5
29	4758.0
30	5576.0
31	6394.0
32	6968.5
33	7543.0
34	9942.5
35	12342.0
36	13415.5
37	14489.0
38	16208.5
39	17928.0
40	21139.0
41	24350.0
42	29992.0
43	35634.0
44	39366.5
45	43099.0
46	50304.0
47	57509.0
48	65794.0
49	74079.0
50	73846.0
51	73613.0
52	62418.0
53	51223.0
54	42905.0
55	34587.0
56	30351.0
57	26115.0
58	23639.0
59	21163.0
60	19626.0
61	18089.0
62	16336.0
63	14583.0
64	12830.0
65	11077.0
66	9231.0
67	7385.0
68	5661.5
69	3938.0
70	3206.0
71	2474.0
72	2129.0
73	1784.0
74	1506.5
75	847.5
76	466.0
77	470.5
78	475.0
79	274.0
80	73.0
81	48.0
82	23.0
83	17.5
84	12.0
85	16.5
86	21.0
87	13.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	593478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.049391932982072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.62148317954089	16.938770352538743
2	12.556599883618306	6.290703836607839
3	5.758369981085415	4.327309996539813
4	3.166879676941138	3.1731364092917684
5	1.9779723290700542	2.4773502051734586
6	1.3753292183362074	2.067069637619132
7	0.9078613525064715	1.5918962387819258
8	0.6684360442911094	1.3395133164464128
9	0.5191653706034528	1.170429916165
>10	3.6792978176877233	20.11988326024452
>50	1.4147482794865158	25.708200353085704
>100	0.3457066127021779	11.50979881995509
>500	0.004075127065251606	0.7769947852352466
>1k	0.004075127065251606	2.508942872315345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	4046	0.6817438894112335	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	3648	0.6146815888710281	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2341	0.3944543858407557	No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	2054	0.34609539022507996	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1556	0.2621832654285416	No Hit
ACTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1102	0.18568506330478973	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	921	0.15518688140082698	No Hit
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG	857	0.14440299387677386	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	799	0.13463009580810073	No Hit
GGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	675	0.1137363137302478	No Hit
AGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	671	0.11306232075999446	No Hit
ACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	644	0.10851286821078455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6849824256333007E-4	0.0	0.0	0.3673261687880595	0.0
2	1.6849824256333007E-4	0.0	0.0	2.2433856014881766	0.0
3	1.6849824256333007E-4	0.0	0.0	3.1467046798701888	0.0
4	1.6849824256333007E-4	0.0	0.0	4.092822311863287	0.0
5	1.6849824256333007E-4	0.0	0.0	6.683314293031923	0.0
6	1.6849824256333007E-4	0.0	0.0	7.971483357428582	0.0
7	1.6849824256333007E-4	0.0	0.0	9.266560849770338	0.0
8	1.6849824256333007E-4	0.0	0.0	11.174971945042612	0.0
9	1.6849824256333007E-4	0.0	0.0	11.854019862572834	0.0
10	1.6849824256333007E-4	0.0	0.0	13.318101092205609	0.0
11	1.6849824256333007E-4	0.0	0.0	15.65129625698004	0.0
12	1.6849824256333007E-4	0.0	0.0	17.411428898796586	0.0
13	1.6849824256333007E-4	0.0	0.0	18.100081216152915	0.0
14	1.6849824256333007E-4	0.0	0.0	18.362095983338893	0.0
15	1.6849824256333007E-4	0.0	0.0	18.96414020401767	0.0
16	1.6849824256333007E-4	0.0	0.0	20.117342176121102	0.0
17	1.6849824256333007E-4	0.0	0.0	21.448646790613974	0.0
18	1.6849824256333007E-4	0.0	0.0	23.00927751323554	0.0
19	1.6849824256333007E-4	0.0	0.0	23.786391407937614	0.0
20	1.6849824256333007E-4	0.0	0.0	24.59619396169698	0.0
21	1.6849824256333007E-4	0.0	0.0	25.58696362796936	0.0
22	1.6849824256333007E-4	0.0	0.0	26.53560873360091	0.0
23	1.6849824256333007E-4	0.0	0.0	27.556371087049563	0.0
24	1.6849824256333007E-4	0.0	0.0	28.28950694044261	0.0
25	1.6849824256333007E-4	0.0	0.0	28.97108233161128	0.0
26	1.6849824256333007E-4	0.0	0.0	29.58273095211617	0.0
27	1.6849824256333007E-4	0.0	0.0	30.1620279100489	0.0
28	1.6849824256333007E-4	0.0	0.0	30.751771759020553	0.0
29	3.3699648512666014E-4	0.0	0.0	31.348929530664996	0.0
30	3.3699648512666014E-4	0.0	0.0	32.07043900532118	0.0
31	3.3699648512666014E-4	0.0	0.0	32.7154502778536	0.0
32	3.3699648512666014E-4	0.0	0.0	33.29441023930121	0.0
33	3.3699648512666014E-4	0.0	0.0	33.888535042579505	0.0
34	3.3699648512666014E-4	0.0	0.0	34.468674491725054	0.0
35	5.054947276899902E-4	0.0	0.0	35.14148797428043	0.0
36	5.054947276899902E-4	0.0	0.0	35.74454318441459	0.0
37	5.054947276899902E-4	0.0	0.0	36.29014049383465	0.0
38	5.054947276899902E-4	0.0	0.0	36.85949605545614	0.0
39	5.054947276899902E-4	0.0	0.0	37.45631683061546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	35	1.210592E-7	45.000004	1
TAGACTC	35	1.210592E-7	45.000004	28
TCTTACG	35	1.210592E-7	45.000004	33
TAAACGG	35	1.210592E-7	45.000004	2
GTCGTCA	35	1.210592E-7	45.000004	20
TATAGCG	35	1.210592E-7	45.000004	1
ATACGAA	95	0.0	45.000004	19
CCACGCG	65	0.0	45.000004	1
GCATCGA	35	1.210592E-7	45.000004	27
AGTCGTC	35	1.210592E-7	45.000004	19
TAATCAC	20	7.0305256E-4	45.0	35
TGATCGG	25	3.8885177E-5	45.0	11
TCACGCA	20	7.0305256E-4	45.0	20
GTACCTT	40	6.8048394E-9	45.0	31
ACCGGCA	20	7.0305256E-4	45.0	4
GTCGATT	25	3.8885177E-5	45.0	16
AGTCATG	20	7.0305256E-4	45.0	24
AGTCAAT	25	3.8885177E-5	45.0	20
TTTACGT	20	7.0305256E-4	45.0	11
GAATGCG	20	7.0305256E-4	45.0	9
>>END_MODULE