FastQCFastQC Report
Sat 14 Jan 2017
SRR2934850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353015
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC62710.4634834055793912No Hit
CCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC43020.31795656367446035No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT40630.30029231013699037No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30110.22254003096787547No Hit
TCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC25980.1920156095830423No Hit
ACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC18720.13835766787507897No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTG14120.10435952299124547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG351.2121382E-745.0000041
CGAAACA207.0337305E-445.019
TAGACCG207.0337305E-445.01
TAGCGTC207.0337305E-445.01
CGCATCG253.8911734E-545.021
CCGTCAC253.8911734E-545.019
CGCTACC207.0337305E-445.037
ACGGATC207.0337305E-445.05
CGTGTCG253.8911734E-545.019
TCGTTAG650.044.9999961
CTCGCGG302.1657888E-644.9999962
CGATCAT302.1657888E-644.99999610
TCTAGCG750.042.01
CGTTTTT24250.041.3814431
GTTAGCG603.6379788E-1241.2499961
AGGGCGC1750.041.1428576
GCGATCA2250.041.09
CGTTGCG556.184564E-1140.9090922
GCGTTGC501.0822987E-940.51
GCGGACC501.0822987E-940.55
Produced by FastQC (version 0.11.5)