FastQCFastQC Report
Sat 14 Jan 2017
SRR2934849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721612
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC38240.5299246686585035TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC36880.5110779754216948TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.46008104078091827No Hit
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT23330.32330393618731396TruSeq Adapter, Index 23 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC15130.20966946225949679TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC10650.14758623747942107TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG9410.13040248776350727TruSeq Adapter, Index 23 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG8880.12305782054622151TruSeq Adapter, Index 23 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG7920.10975427237906243TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGG253.889358E-545.02
TCGCCCG207.03154E-445.029
AACGTTA207.03154E-445.024
CGGAATC351.2110831E-745.027
CTAGACG253.889358E-545.01
TAATACG253.889358E-545.01
GATACGT207.03154E-445.018
AATCCCG207.03154E-445.016
GGTACGA207.03154E-445.09
CGAACAA253.889358E-545.025
ATTAGGC207.03154E-445.020
CTCAACG253.889358E-545.043
CGTTGTC207.03154E-445.023
CCGGATC207.03154E-445.043
GTTAGCG700.045.01
TACGGTA207.03154E-445.01
AAGCGGT253.889358E-545.012
TCGATTT207.03154E-445.042
CCGCTCG351.2110831E-745.019
TAGCCGA207.03154E-445.031
Produced by FastQC (version 0.11.5)