##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934849.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721612
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47117010249275	33.0	31.0	34.0	31.0	34.0
2	32.659150623880976	34.0	31.0	34.0	31.0	34.0
3	32.64332355892086	34.0	31.0	34.0	31.0	34.0
4	36.15530229541638	37.0	35.0	37.0	35.0	37.0
5	36.055170922878226	37.0	35.0	37.0	35.0	37.0
6	35.99527446882812	37.0	35.0	37.0	35.0	37.0
7	36.22936425669196	37.0	36.0	37.0	35.0	37.0
8	36.06922972456112	37.0	37.0	37.0	35.0	37.0
9	37.83821638221094	39.0	38.0	39.0	35.0	39.0
10	37.61522258499027	39.0	37.0	39.0	35.0	39.0
11	37.50429455164271	39.0	37.0	39.0	35.0	39.0
12	37.35722382665477	39.0	37.0	39.0	35.0	39.0
13	37.25970050387188	39.0	37.0	39.0	34.0	39.0
14	38.443839625726845	40.0	38.0	41.0	34.0	41.0
15	38.53282234774366	40.0	38.0	41.0	35.0	41.0
16	38.63033735580894	40.0	38.0	41.0	35.0	41.0
17	38.6359012876726	40.0	38.0	41.0	35.0	41.0
18	38.57958847691003	40.0	38.0	41.0	35.0	41.0
19	38.55424106029279	40.0	37.0	41.0	35.0	41.0
20	38.49308076916681	40.0	37.0	41.0	35.0	41.0
21	38.437311186621066	40.0	37.0	41.0	35.0	41.0
22	38.37137686180385	40.0	37.0	41.0	34.0	41.0
23	38.214185739704995	40.0	36.0	41.0	34.0	41.0
24	37.97251015781334	40.0	36.0	41.0	34.0	41.0
25	37.91971585838373	40.0	36.0	41.0	34.0	41.0
26	38.06712471522092	40.0	36.0	41.0	34.0	41.0
27	38.1712651674307	40.0	37.0	41.0	34.0	41.0
28	38.16709117919325	40.0	37.0	41.0	34.0	41.0
29	38.177125657555585	40.0	36.0	41.0	34.0	41.0
30	38.064680188245205	40.0	36.0	41.0	34.0	41.0
31	37.96286369960588	40.0	36.0	41.0	34.0	41.0
32	37.85865672965527	40.0	36.0	41.0	34.0	41.0
33	37.72905938371313	40.0	36.0	41.0	34.0	41.0
34	37.678662494526144	40.0	36.0	41.0	34.0	41.0
35	37.59583266353664	40.0	36.0	41.0	33.0	41.0
36	37.53148090663681	40.0	36.0	41.0	33.0	41.0
37	37.46762803279324	40.0	36.0	41.0	33.0	41.0
38	37.38806727160856	40.0	35.0	41.0	33.0	41.0
39	37.386287922041205	40.0	36.0	41.0	33.0	41.0
40	37.297217895489545	40.0	35.0	41.0	33.0	41.0
41	37.238833334257194	40.0	35.0	41.0	33.0	41.0
42	37.17565395253959	40.0	35.0	41.0	33.0	41.0
43	37.10632167979468	39.0	35.0	41.0	33.0	41.0
44	37.04114260849321	39.0	35.0	41.0	33.0	41.0
45	36.97626563859803	39.0	35.0	41.0	32.0	41.0
46	36.89568493872053	39.0	35.0	41.0	32.0	41.0
47	36.78442154509626	39.0	35.0	41.0	32.0	41.0
48	36.756439748784665	39.0	35.0	41.0	32.0	41.0
49	36.743662799399125	39.0	35.0	41.0	32.0	41.0
50	36.626494570489406	39.0	35.0	41.0	32.0	41.0
51	35.74506937246055	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	13.0
10	11.0
11	14.0
12	15.0
13	8.0
14	16.0
15	23.0
16	35.0
17	51.0
18	125.0
19	230.0
20	345.0
21	545.0
22	892.0
23	1451.0
24	2182.0
25	3307.0
26	4649.0
27	5344.0
28	5822.0
29	6234.0
30	6973.0
31	9100.0
32	12446.0
33	18534.0
34	40613.0
35	61703.0
36	48237.0
37	80398.0
38	157429.0
39	254658.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.41938326967956	21.993120956968564	25.705642367366394	12.881853405985488
2	33.05515983658808	24.352283498611442	24.687920932578727	17.90463573222175
3	31.022765696801052	23.95885877729306	27.796239530384746	17.22213599552114
4	28.546642794188564	24.640804199486706	27.58130408030909	19.231248926015642
5	24.140396778324085	29.434516055719694	26.49346186039035	19.931625305565873
6	25.098945139493246	33.32039932817082	26.2466533261642	15.334002206171737
7	79.40818611663886	5.343730425768973	10.199525506782038	5.048557950810131
8	79.98786051229747	3.9468578682172692	9.946342355725792	6.118939263759471
9	74.70940061972362	5.413574053646558	12.285133839237707	7.591891487392116
10	40.209420020731365	24.734206193910303	20.036806483262474	15.019567302095863
11	28.166244463783862	24.438617983071236	26.22545079627279	21.169686756872114
12	25.89923670892391	22.724538948908833	31.467741667267173	19.908482674900085
13	21.99145801344767	26.05001025481838	32.67379145579619	19.28474027593776
14	18.188721917041292	29.075043097952918	30.902202291536174	21.83403269346962
15	15.972711096822115	26.248454848311837	37.11329634207857	20.665537712787483
16	18.709916132215096	25.688874353530704	33.87623820002993	21.724971314224266
17	19.09585760768945	24.38872967744439	29.574757625981828	26.94065508888433
18	20.198666319296244	25.107814171604687	32.80696551609452	21.88655399300455
19	21.243272007671713	26.379688807835787	29.633099227839892	22.743939956652607
20	22.589840523716344	26.748862269474454	29.65208449970344	21.009212707105757
21	21.35787653198672	26.852796239530385	31.23950266902435	20.549824559458546
22	20.346529714029145	24.947617279091812	28.65611990931415	26.049733097564896
23	19.537369112487042	26.004695043873994	29.856626552773513	24.60130929086545
24	21.010321336119688	24.017477536404606	31.348286891016226	23.62391423645948
25	18.77851255245201	26.588388219708097	29.1821643764239	25.450934851415997
26	17.986812857879304	28.27641447204315	30.409832430724542	23.326940239353004
27	20.015881110624544	26.512031396373676	29.437841942761484	24.034245550240296
28	16.713136699500563	25.336746062981213	32.61725137608577	25.332865861432456
29	19.575616813467626	23.550606142913367	32.553366629158056	24.32041041446096
30	20.606087481915488	24.717161022821127	29.176898388607732	25.499853106655657
31	21.128251747476483	25.032427398657454	27.942439981596756	25.896880872269307
32	21.901659063319347	25.35074250428208	28.808417820102772	23.939180612295804
33	20.87770159032832	24.03479986474726	28.495091545040825	26.592406999883593
34	18.671807009861254	22.179647788562274	32.54810064134189	26.60044456023459
35	18.788905949457604	22.623237972760986	32.472436711141164	26.115419366640246
36	20.50478650576764	22.26972389594408	32.05406783700936	25.171421761278918
37	19.83739183938183	24.346878932168533	32.105480507530366	23.710248720919274
38	19.720708635665705	24.711340720497997	29.650005820302322	25.91794482353398
39	21.256159819958647	23.477990942500956	30.59275621802298	24.673093019517413
40	21.008104078091826	21.671341385675404	33.719782930439074	23.600771605793692
41	17.906160097115905	23.49406606320294	32.484493051667656	26.1152807880135
42	20.301630238964986	22.099965078186063	32.46051894924142	25.137885733607533
43	21.381712055786213	23.004190617672656	30.24284518550135	25.371252141039786
44	20.33031601470042	23.281763607035362	28.49717022444194	27.890750153822275
45	20.643780868389104	22.72232169088097	28.214469825889815	28.41942761484011
46	22.125047809626224	22.40927257307251	30.707222163711243	24.75845745359002
47	18.542928886991902	23.251137730525546	33.91420874375704	24.29172463872552
48	18.98194597650815	21.39834149099516	33.293237917329535	26.326474615167157
49	20.004656241858505	19.23845501460619	34.52617195944635	26.23071678408896
50	20.046922723014585	20.326435813151665	32.64441278692705	26.982228676906704
51	18.66266082049633	20.48108956059489	30.457087742443306	30.39916187646547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	450.5
2	609.0
3	3043.5
4	5478.0
5	3629.5
6	1781.0
7	1735.0
8	1689.0
9	1805.5
10	1922.0
11	1926.0
12	1930.0
13	2007.5
14	2085.0
15	2075.0
16	2065.0
17	1964.0
18	1863.0
19	1861.0
20	1859.0
21	2101.0
22	2343.0
23	2717.5
24	3092.0
25	3234.5
26	4566.5
27	5756.0
28	6712.5
29	7669.0
30	8539.5
31	9410.0
32	11102.0
33	12794.0
34	15331.5
35	17869.0
36	19538.0
37	21207.0
38	22521.5
39	23836.0
40	27759.5
41	31683.0
42	37121.0
43	42559.0
44	46496.5
45	50434.0
46	59757.5
47	69081.0
48	79547.5
49	90014.0
50	86652.0
51	83290.0
52	69764.5
53	56239.0
54	49256.5
55	42274.0
56	36372.0
57	30470.0
58	28119.0
59	25768.0
60	23424.5
61	21081.0
62	19426.5
63	17772.0
64	14311.5
65	10851.0
66	9091.0
67	7331.0
68	6255.0
69	5179.0
70	4338.0
71	3497.0
72	2958.0
73	2419.0
74	1868.5
75	1027.0
76	736.0
77	535.5
78	335.0
79	266.0
80	197.0
81	149.5
82	102.0
83	59.0
84	16.0
85	23.5
86	31.0
87	17.5
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	721612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.614315549997265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.41999061673606	15.68462617251463
2	13.281332655224132	6.272591604898913
3	5.843757883769336	4.1398902799534
4	3.2307180036348115	3.0516477756355864
5	1.9760952559581484	2.3332068465524167
6	1.3108877355637476	1.8573429982934226
7	0.9258837190134779	1.530487721237249
8	0.6256122647043754	1.1818724344542029
9	0.5115722987798518	1.0872386721022413
>10	3.826235880179481	20.672502456676337
>50	1.7270916990052398	29.404528022502202
>100	0.31191026555822166	9.699093157463624
>500	0.005347033123855228	0.8762897004544544
>1k	0.0035646887492368187	2.2086821572613635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	3824	0.5299246686585035	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	3688	0.5110779754216948	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3320	0.46008104078091827	No Hit
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	2333	0.32330393618731396	TruSeq Adapter, Index 23 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1513	0.20966946225949679	TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1065	0.14758623747942107	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG	941	0.13040248776350727	TruSeq Adapter, Index 23 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG	888	0.12305782054622151	TruSeq Adapter, Index 23 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG	792	0.10975427237906243	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3350831194603194	0.0
2	0.0	0.0	0.0	1.7988891537280423	0.0
3	0.0	0.0	0.0	2.5984878300249994	0.0
4	0.0	0.0	0.0	3.479432160219065	0.0
5	0.0	0.0	0.0	5.794110962678004	0.0
6	0.0	0.0	0.0	6.988658725187497	0.0
7	0.0	0.0	0.0	8.227135912373964	0.0
8	0.0	0.0	0.0	9.912252013547446	0.0
9	0.0	0.0	0.0	10.537657356030664	0.0
10	0.0	0.0	0.0	11.99841466051008	0.0
11	0.0	0.0	0.0	14.376008159509542	0.0
12	0.0	0.0	0.0	16.096046074621817	0.0
13	0.0	0.0	0.0	16.73281486449782	0.0
14	0.0	0.0	0.0	16.971447259746235	0.0
15	0.0	0.0	0.0	17.441921697532745	0.0
16	0.0	0.0	0.0	18.436223344401146	0.0
17	0.0	0.0	0.0	19.659734039899558	0.0
18	0.0	0.0	0.0	20.949485318980283	0.0
19	0.0	0.0	0.0	21.715963703486082	0.0
20	0.0	0.0	0.0	22.44267002211715	0.0
21	0.0	0.0	0.0	23.356318908222146	0.0
22	0.0	0.0	0.0	24.30281092886482	0.0
23	0.0	0.0	0.0	25.23350498605899	0.0
24	0.0	0.0	0.0	25.986125507890666	0.0
25	0.0	0.0	0.0	26.604324761783342	0.0
26	0.0	0.0	0.0	27.174298653570062	0.0
27	0.0	0.0	0.0	27.696047183250833	0.0
28	0.0	0.0	0.0	28.273781478135064	0.0
29	0.0	0.0	0.0	28.870501044882847	0.0
30	0.0	0.0	0.0	29.506992677505362	0.0
31	0.0	0.0	0.0	30.116045742033116	0.0
32	0.0	0.0	0.0	30.67507192230728	0.0
33	1.3857862674124044E-4	0.0	0.0	31.21247983680981	0.0
34	1.3857862674124044E-4	0.0	0.0	31.753352216980872	0.0
35	1.3857862674124044E-4	0.0	0.0	32.328453517957016	0.0
36	1.3857862674124044E-4	0.0	0.0	32.83232540478817	0.0
37	1.3857862674124044E-4	0.0	0.0	33.35989423679207	0.0
38	1.3857862674124044E-4	0.0	0.0	33.87194226260095	0.0
39	1.3857862674124044E-4	0.0	0.0	34.40741007632911	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	25	3.889358E-5	45.0	2
TCGCCCG	20	7.03154E-4	45.0	29
AACGTTA	20	7.03154E-4	45.0	24
CGGAATC	35	1.2110831E-7	45.0	27
CTAGACG	25	3.889358E-5	45.0	1
TAATACG	25	3.889358E-5	45.0	1
GATACGT	20	7.03154E-4	45.0	18
AATCCCG	20	7.03154E-4	45.0	16
GGTACGA	20	7.03154E-4	45.0	9
CGAACAA	25	3.889358E-5	45.0	25
ATTAGGC	20	7.03154E-4	45.0	20
CTCAACG	25	3.889358E-5	45.0	43
CGTTGTC	20	7.03154E-4	45.0	23
CCGGATC	20	7.03154E-4	45.0	43
GTTAGCG	70	0.0	45.0	1
TACGGTA	20	7.03154E-4	45.0	1
AAGCGGT	25	3.889358E-5	45.0	12
TCGATTT	20	7.03154E-4	45.0	42
CCGCTCG	35	1.2110831E-7	45.0	19
TAGCCGA	20	7.03154E-4	45.0	31
>>END_MODULE