FastQCFastQC Report
Sat 14 Jan 2017
SRR2934847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029307
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC49330.2430879112918844TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.18099774947802377No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC31800.1567037417206958TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC22860.11264929357657566No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT22590.11131879010913578TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC21540.10614460995798072No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA207.0345646E-445.021
CGTAAAC207.0345646E-445.044
TCGCGGA207.0345646E-445.024
TCGATAG1300.039.8076931
TATCGTG301.1402301E-437.4999961
CGTTTTT26650.037.3170741
CGTAGCG552.752131E-936.818182
TACGAAT2150.036.62790712
TTATGCG1000.036.01
TGCGATA250.00210748836.01
TCGTAAT250.00210748836.043
CGTAACG250.00210748836.033
GCGTTAG2150.035.5813941
GCGATGT4050.035.5555579
TTGGACG1600.035.156251
CGAGATA2950.035.08474719
TCCGATC458.704301E-735.014
GATTACG900.035.01
TATAACG458.704301E-735.01
CGAATAT2200.034.77272814
Produced by FastQC (version 0.11.5)