##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934847.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2029307
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35751465894515	33.0	31.0	34.0	31.0	34.0
2	32.53688722307665	34.0	31.0	34.0	31.0	34.0
3	32.54010950536316	34.0	31.0	34.0	31.0	34.0
4	36.04249135295941	37.0	35.0	37.0	35.0	37.0
5	35.93942365546465	37.0	35.0	37.0	35.0	37.0
6	35.8741575325961	37.0	35.0	37.0	35.0	37.0
7	36.1252664086804	37.0	35.0	37.0	35.0	37.0
8	35.93979077586585	37.0	35.0	37.0	35.0	37.0
9	37.62808830797903	39.0	38.0	39.0	35.0	39.0
10	37.378531685940075	39.0	37.0	39.0	34.0	39.0
11	37.28879711152625	39.0	37.0	39.0	34.0	39.0
12	37.189348876241986	39.0	37.0	39.0	34.0	39.0
13	37.17839636880965	39.0	37.0	39.0	34.0	39.0
14	38.4046159600297	40.0	38.0	41.0	34.0	41.0
15	38.45850135046102	40.0	38.0	41.0	34.0	41.0
16	38.53422424502552	40.0	38.0	41.0	34.0	41.0
17	38.49765264693809	40.0	38.0	41.0	34.0	41.0
18	38.480170324155	40.0	38.0	41.0	34.0	41.0
19	38.45999151434455	40.0	38.0	41.0	34.0	41.0
20	38.41885185435225	40.0	37.0	41.0	34.0	41.0
21	38.35423767818275	40.0	37.0	41.0	34.0	41.0
22	38.30075685936135	40.0	37.0	41.0	34.0	41.0
23	38.133369667576176	40.0	37.0	41.0	34.0	41.0
24	37.88936321611269	40.0	36.0	41.0	33.0	41.0
25	37.826306221779156	40.0	36.0	41.0	33.0	41.0
26	37.9667763428599	40.0	37.0	41.0	34.0	41.0
27	38.0225313370525	40.0	37.0	41.0	34.0	41.0
28	37.971015228351355	40.0	37.0	41.0	34.0	41.0
29	37.98324501911244	40.0	37.0	41.0	34.0	41.0
30	37.883214811755934	40.0	37.0	41.0	33.0	41.0
31	37.83662550811681	40.0	36.0	41.0	33.0	41.0
32	37.764310180766145	40.0	36.0	41.0	33.0	41.0
33	37.6776776505477	40.0	36.0	41.0	33.0	41.0
34	37.62042855023907	40.0	36.0	41.0	33.0	41.0
35	37.55727398565126	40.0	36.0	41.0	33.0	41.0
36	37.50097841282763	40.0	36.0	41.0	33.0	41.0
37	37.45499966244634	40.0	36.0	41.0	33.0	41.0
38	37.38931467737508	40.0	36.0	41.0	33.0	41.0
39	37.37814879660889	40.0	36.0	41.0	33.0	41.0
40	37.293237543654065	40.0	35.0	41.0	33.0	41.0
41	37.20983665852432	40.0	35.0	41.0	32.0	41.0
42	37.10823005094843	40.0	35.0	41.0	32.0	41.0
43	37.055528808603135	39.0	35.0	41.0	32.0	41.0
44	37.011060426046924	39.0	35.0	41.0	32.0	41.0
45	36.96697444004283	39.0	35.0	41.0	32.0	41.0
46	36.907612795895346	39.0	35.0	41.0	32.0	41.0
47	36.805557266593965	39.0	35.0	41.0	31.0	41.0
48	36.74782622836269	39.0	35.0	41.0	31.0	41.0
49	36.69633475861464	39.0	35.0	41.0	31.0	41.0
50	36.60587087118903	39.0	35.0	41.0	31.0	41.0
51	35.6977234100114	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	30.0
10	44.0
11	36.0
12	39.0
13	45.0
14	61.0
15	84.0
16	145.0
17	267.0
18	443.0
19	793.0
20	1371.0
21	2171.0
22	3459.0
23	4878.0
24	6876.0
25	9484.0
26	12648.0
27	15333.0
28	17611.0
29	20718.0
30	25806.0
31	32394.0
32	42552.0
33	60186.0
34	113140.0
35	148398.0
36	139112.0
37	217929.0
38	411740.0
39	740865.0
40	633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.790916800661506	21.478021807444613	25.266655069932742	11.464406321961143
2	32.030343363522626	24.61628526388565	25.542069287692794	17.811302084898934
3	31.68416607245725	24.634320977555394	26.080331857131522	17.601181092855835
4	28.52382611403794	25.859468281536508	26.751694051220444	18.865011553205107
5	25.753915006452942	30.0641549060837	24.562917291469454	19.619012795993903
6	26.658213863156238	34.90053501022763	23.47633945972689	14.964911666889238
7	82.32056559209622	5.449101589853088	7.665473977076903	4.564858840973791
8	83.98374420430225	4.781139571292072	6.779703613105361	4.455412611300311
9	78.56056279311115	6.451217090366317	8.718542832602461	6.269677283920077
10	40.16445022857557	27.27679941970338	17.23484913815406	15.323901213566996
11	29.76134217247563	26.85414281821331	24.551583373043112	18.832931636267947
12	27.706108538530643	23.290955976596937	28.94909444455669	20.053841040315735
13	24.78644187399935	23.678428153059148	29.98156513529003	21.55356483765147
14	20.685928743162073	26.885582122369854	29.252597068851582	23.175892065616488
15	19.79882787572309	26.89484636873573	32.52445292900482	20.78187282653635
16	22.678480880418782	25.605046451818282	30.573885567831777	21.14258709993116
17	22.598059337497972	26.031990231147873	28.20233705397951	23.16761337737464
18	23.12094720020184	26.337759639128038	28.50790935033487	22.033383810335252
19	23.76826177606444	28.008871994232514	26.788898870402555	21.43396735930049
20	26.16533624532907	26.40453120203104	27.599175482073434	19.830957070566456
21	25.342543045483012	27.205543567336044	27.193519758222877	20.258393628958064
22	23.41725525019132	24.85617011127444	27.607109224971875	24.119465413562363
23	23.16450886928395	26.202294674980177	28.275761134219714	22.357435321516164
24	23.365316337054963	24.843801356817867	29.055485444045676	22.73539686208149
25	22.00928691420273	27.009762445997577	26.704978596141444	24.275972043658253
26	21.626988917891673	27.923818328128764	27.427737646398498	23.02145510758106
27	22.22251241433652	27.278031367358412	28.210467908502757	22.288988309802313
28	20.285397921556473	27.42803331383571	29.144777010082752	23.14179175452507
29	21.516852797531374	25.42094419424956	28.642142366827688	24.420060641391373
30	22.35304958786423	25.684038935459245	29.069677481031704	22.893233995644817
31	21.946063360546237	26.555518706632363	27.4844565164364	24.013961416385
32	22.571005766993363	26.601347159399737	27.247134120170085	23.580512953436813
33	22.798620415737982	26.02213464990758	28.195536702923707	22.98370823143073
34	21.01214848221585	25.34071976295356	28.933966127352832	24.713165627477753
35	21.342310453765744	24.748152940880804	29.01882268183178	24.890713923521677
36	21.489207892152347	26.711138334416628	28.759620895212013	23.04003287821902
37	21.76861362031472	26.246595512655308	29.42566107543117	22.559129791598806
38	21.45057401369039	25.84143256786676	28.695658172962496	24.012335245480354
39	22.981835670995075	24.80245719351483	28.20603289694462	24.009674238545472
40	22.31929422211622	24.21284704581416	30.266637822665572	23.201220909404046
41	20.323982522112228	25.534135544794356	29.574923853315443	24.566958079777972
42	22.00761146539188	24.894064821143374	29.04149051868446	24.056833194780285
43	22.398927318537805	24.87464932609999	28.85901443202039	23.86740892334181
44	21.242916916957366	25.09590712494462	28.392056992855196	25.269118965242814
45	21.78571305376663	24.00923073738966	28.10949747869593	26.09555873014778
46	21.69164153082801	25.36698488695895	28.09954334164323	24.84183024056981
47	20.506064385526685	25.204663463931283	30.629224656496035	23.660047494045997
48	21.327576359811502	23.31416587041783	30.444678897771503	24.91357887199916
49	21.10868390046454	23.78339009326829	30.490852295882288	24.617073710384876
50	20.015650663009588	23.464463484332338	30.49711058996988	26.022775262688196
51	19.803903500061843	23.613874095935213	28.92342065542572	27.658801748577222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	394.0
1	1065.0
2	1736.0
3	4601.5
4	7467.0
5	5248.5
6	3030.0
7	3029.5
8	3029.0
9	2995.5
10	2962.0
11	3020.5
12	3079.0
13	3066.0
14	3053.0
15	3232.0
16	3411.0
17	3539.5
18	3668.0
19	4013.0
20	4358.0
21	4842.0
22	5326.0
23	6976.0
24	8626.0
25	9961.0
26	15333.0
27	19370.0
28	22293.0
29	25216.0
30	30262.0
31	35308.0
32	40054.5
33	44801.0
34	49818.5
35	54836.0
36	61830.0
37	68824.0
38	75419.0
39	82014.0
40	89104.5
41	96195.0
42	105641.5
43	115088.0
44	124838.0
45	134588.0
46	152262.5
47	169937.0
48	179645.0
49	189353.0
50	186544.0
51	183735.0
52	163199.5
53	142664.0
54	127772.0
55	112880.0
56	106342.0
57	99804.0
58	96486.0
59	93168.0
60	90583.5
61	87999.0
62	81340.5
63	74682.0
64	62887.5
65	51093.0
66	41583.0
67	32073.0
68	26872.5
69	21672.0
70	17442.5
71	13213.0
72	11139.0
73	9065.0
74	6854.5
75	3707.0
76	2770.0
77	2295.0
78	1820.0
79	1266.0
80	712.0
81	443.5
82	175.0
83	122.0
84	69.0
85	58.5
86	48.0
87	32.0
88	16.0
89	17.5
90	19.0
91	13.0
92	7.0
93	8.0
94	9.0
95	6.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2029307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.919348820404732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.09733117408236	15.584072022045467
2	11.468644647958207	5.027704450717276
3	4.336675923273797	2.8517133684986735
4	2.1360348378973293	1.8728197081763296
5	1.2693740219102987	1.391192598490596
6	0.8385562796929431	1.1028364560078296
7	0.6029580435628555	0.9251513376646117
8	0.429697384810105	0.7534949491894699
9	0.35250803378068596	0.6954071898990484
>10	5.087362318567814	28.381044694736325
>50	2.154323765065903	33.02493544635041
>100	0.2207956394885838	6.590086477968514
>500	0.0029837235527623443	0.4310447263234315
>1k	0.00275420635639601	1.368496573932071
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	4933	0.2430879112918844	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3673	0.18099774947802377	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	3180	0.1567037417206958	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	2286	0.11264929357657566	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	2259	0.11131879010913578	TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	2154	0.10614460995798072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.942232496118133E-4	0.0	0.0	0.11772491791532774	0.0
2	3.942232496118133E-4	0.0	0.0	0.6960011471896563	0.0
3	3.942232496118133E-4	0.0	0.0	1.0242412803976924	0.0
4	4.4350115581328996E-4	0.0	0.0	1.4112699557040902	0.0
5	4.4350115581328996E-4	0.0	0.0	2.5225852963597917	0.0
6	4.4350115581328996E-4	0.0	0.0	3.158221008452639	0.0
7	4.4350115581328996E-4	0.0	0.0	3.750048661932374	0.0
8	4.4350115581328996E-4	0.0	0.0	4.672038286962002	0.0
9	4.4350115581328996E-4	0.0	0.0	4.984213822748357	0.0
10	4.4350115581328996E-4	0.0	0.0	5.828787857135466	0.0
11	4.4350115581328996E-4	0.0	0.0	6.978638520440722	0.0
12	4.4350115581328996E-4	0.0	0.0	7.921029198637761	0.0
13	4.4350115581328996E-4	0.0	0.0	8.25838574449307	0.0
14	4.4350115581328996E-4	0.0	0.0	8.396363881857205	0.0
15	4.4350115581328996E-4	0.0	0.0	8.678430616954458	0.0
16	4.4350115581328996E-4	0.0	0.0	9.26986404718458	0.0
17	4.4350115581328996E-4	0.0	0.0	9.944429305176595	0.0
18	4.4350115581328996E-4	0.0	0.0	10.655115268414292	0.0
19	4.4350115581328996E-4	0.0	0.0	11.131879010913577	0.0
20	4.4350115581328996E-4	0.0	0.0	11.564292637831535	0.0
21	4.4350115581328996E-4	0.0	0.0	12.140597750857806	0.0
22	4.4350115581328996E-4	0.0	0.0	12.766427159616558	0.0
23	4.4350115581328996E-4	0.0	0.0	13.367075558306357	0.0
24	4.927790620147666E-4	0.0	0.0	13.847436587958352	0.0
25	4.927790620147666E-4	0.0	0.0	14.280934328812743	0.0
26	4.927790620147666E-4	0.0	0.0	14.669835564554797	0.0
27	4.927790620147666E-4	0.0	0.0	15.044249095873615	0.0
28	4.927790620147666E-4	0.0	0.0	15.440985518701705	0.0
29	4.927790620147666E-4	0.0	0.0	15.859699887695651	0.0
30	4.927790620147666E-4	0.0	0.0	16.315964021215123	0.0
31	5.9133487441772E-4	0.0	0.0	16.737487230862556	0.0
32	5.9133487441772E-4	0.0	0.0	17.174089479807638	0.0
33	5.9133487441772E-4	0.0	0.0	17.580730761782224	0.0
34	5.9133487441772E-4	0.0	0.0	17.991117164628122	0.0
35	5.9133487441772E-4	0.0	0.0	18.414069433555397	0.0
36	5.9133487441772E-4	0.0	0.0	18.842245160540028	0.0
37	5.9133487441772E-4	0.0	0.0	19.261649420220795	0.0
38	6.406127806191966E-4	0.0	0.0	19.695097883168984	0.0
39	6.406127806191966E-4	0.0	0.0	20.113664418444326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	20	7.0345646E-4	45.0	21
CGTAAAC	20	7.0345646E-4	45.0	44
TCGCGGA	20	7.0345646E-4	45.0	24
TCGATAG	130	0.0	39.807693	1
TATCGTG	30	1.1402301E-4	37.499996	1
CGTTTTT	2665	0.0	37.317074	1
CGTAGCG	55	2.752131E-9	36.81818	2
TACGAAT	215	0.0	36.627907	12
TTATGCG	100	0.0	36.0	1
TGCGATA	25	0.002107488	36.0	1
TCGTAAT	25	0.002107488	36.0	43
CGTAACG	25	0.002107488	36.0	33
GCGTTAG	215	0.0	35.581394	1
GCGATGT	405	0.0	35.555557	9
TTGGACG	160	0.0	35.15625	1
CGAGATA	295	0.0	35.084747	19
TCCGATC	45	8.704301E-7	35.0	14
GATTACG	90	0.0	35.0	1
TATAACG	45	8.704301E-7	35.0	1
CGAATAT	220	0.0	34.772728	14
>>END_MODULE