##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934846.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1769644
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36490729208813	33.0	31.0	34.0	31.0	34.0
2	32.545835207533266	34.0	31.0	34.0	31.0	34.0
3	32.53524889751837	34.0	31.0	34.0	31.0	34.0
4	36.05054293405905	37.0	35.0	37.0	35.0	37.0
5	35.95550178453971	37.0	35.0	37.0	35.0	37.0
6	35.89547615226565	37.0	35.0	37.0	35.0	37.0
7	36.1577673249535	37.0	35.0	37.0	35.0	37.0
8	36.00479587984928	37.0	36.0	37.0	35.0	37.0
9	37.648899439661314	39.0	38.0	39.0	35.0	39.0
10	37.43949178478835	39.0	37.0	39.0	35.0	39.0
11	37.323440194751036	39.0	37.0	39.0	34.0	39.0
12	37.333011046289535	39.0	37.0	39.0	34.0	39.0
13	37.36266051250986	39.0	37.0	39.0	34.0	39.0
14	38.62010381749098	40.0	38.0	41.0	35.0	41.0
15	38.67522111791976	40.0	38.0	41.0	35.0	41.0
16	38.72925684487954	40.0	38.0	41.0	35.0	41.0
17	38.705979846794044	40.0	38.0	41.0	35.0	41.0
18	38.651310094007606	40.0	38.0	41.0	35.0	41.0
19	38.60347278887731	40.0	38.0	41.0	35.0	41.0
20	38.53137636722414	40.0	38.0	41.0	34.0	41.0
21	38.48362325981949	40.0	38.0	41.0	34.0	41.0
22	38.427600692568674	40.0	38.0	41.0	34.0	41.0
23	38.2420339910174	40.0	37.0	41.0	34.0	41.0
24	38.02634089116229	40.0	37.0	41.0	34.0	41.0
25	37.94705714821738	40.0	37.0	41.0	33.0	41.0
26	38.0521754657999	40.0	37.0	41.0	34.0	41.0
27	38.12096952833451	40.0	37.0	41.0	34.0	41.0
28	38.078722048050345	40.0	37.0	41.0	34.0	41.0
29	38.074660214144764	40.0	37.0	41.0	34.0	41.0
30	37.962883495211464	40.0	37.0	41.0	34.0	41.0
31	37.86641720029565	40.0	37.0	41.0	33.0	41.0
32	37.746332595708516	40.0	37.0	41.0	33.0	41.0
33	37.59757725282599	40.0	37.0	41.0	33.0	41.0
34	37.482985843480385	40.0	37.0	41.0	33.0	41.0
35	37.380151036027584	40.0	37.0	41.0	33.0	41.0
36	37.3234294581283	40.0	36.0	41.0	32.0	41.0
37	37.238065396203986	40.0	36.0	41.0	32.0	41.0
38	37.174090947105746	40.0	36.0	41.0	32.0	41.0
39	37.17860089373908	40.0	36.0	41.0	32.0	41.0
40	37.0786378503247	40.0	36.0	41.0	31.0	41.0
41	37.01495159478404	40.0	36.0	41.0	31.0	41.0
42	36.922101846473076	40.0	36.0	41.0	31.0	41.0
43	36.892825901706786	40.0	35.0	41.0	31.0	41.0
44	36.844115539622656	40.0	35.0	41.0	31.0	41.0
45	36.808976833758656	39.0	35.0	41.0	31.0	41.0
46	36.76364511732303	39.0	35.0	41.0	31.0	41.0
47	36.64515066307121	39.0	35.0	41.0	31.0	41.0
48	36.58757806654898	39.0	35.0	41.0	31.0	41.0
49	36.54522265495207	39.0	35.0	41.0	31.0	41.0
50	36.433252111724165	39.0	35.0	41.0	30.0	41.0
51	35.5505225909844	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	22.0
10	32.0
11	26.0
12	27.0
13	21.0
14	31.0
15	52.0
16	108.0
17	184.0
18	325.0
19	633.0
20	1220.0
21	2070.0
22	3154.0
23	4736.0
24	7104.0
25	11179.0
26	15306.0
27	17373.0
28	17831.0
29	19121.0
30	22059.0
31	27315.0
32	34907.0
33	47941.0
34	83663.0
35	113761.0
36	119221.0
37	188705.0
38	366557.0
39	664485.0
40	461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.351449218034816	21.321859085782226	25.439805972274648	12.886885723908312
2	33.10016025822143	23.440251259575373	26.178316090693947	17.281272391509255
3	30.607512019366606	23.653062423854742	29.115799561945792	16.623625994832857
4	28.36949126490978	25.57226199167742	27.903973906616248	18.154272836796554
5	25.44054058330376	29.90273749974571	26.064677415344555	18.592044501605972
6	25.86248985671694	34.84469192673781	24.260246693685282	15.032571522859964
7	81.83103494262123	4.6109273955665655	9.412175556213565	4.14586210559864
8	84.64148721437759	3.8172649414232462	7.767720513278377	3.7735273309207953
9	77.8733462775564	7.3830103681870485	9.584187554106927	5.159455800149634
10	36.86617195322901	33.924506849965304	17.035629765082692	12.173691431722991
11	24.355576601847602	25.73602374262846	31.221081754296343	18.68731790122759
12	24.124061110596255	22.925967030657013	32.489755001570934	20.460216857175794
13	23.334128220139192	23.180368480892202	33.72700950021586	19.758493798752745
14	19.70989645375002	27.37934861474963	31.75265759666916	21.158097334831186
15	18.74094450635269	27.616458451530363	33.5346544276702	20.107942614446745
16	21.841681151689265	26.90292510810084	31.916984433027206	19.338409307182687
17	21.900902102343746	26.753177475243607	30.051411470329626	21.294508952083017
18	21.592421978657857	26.726109884247904	31.272561034874812	20.408907102219427
19	21.46318694607503	28.176401581335003	29.54712925311532	20.81328221947465
20	23.59321987925255	27.426194194990632	29.940541713474573	19.040044212282243
21	23.392727576845964	26.821326775328824	30.55846260603828	19.227483041786936
22	22.086137098761107	25.198344977859954	29.94297158072471	22.77254634265423
23	20.95155861856961	27.17987346607566	30.534842036025324	21.3337258793294
24	21.086614030844622	25.86203778839134	31.54363250461675	21.507715676147292
25	20.701508325968387	27.933019296536475	29.551197868045776	21.81427450944936
26	20.693823164433073	29.318156646195504	29.04787629602338	20.94014389334804
27	21.544050667817935	28.344853541164213	29.690943489198958	20.4201523018189
28	20.267183682141717	28.35010883544939	30.228509236886065	21.154198245522828
29	20.973088372576633	26.350836665453613	30.28513079466831	22.390944167301445
30	22.37851228834726	26.805447875391884	29.46270549330826	21.353334342952593
31	21.993463092011726	28.119497480849255	28.0820888269053	21.80495060023372
32	22.352574868165576	28.836929913587138	27.491857119285008	21.31863809896228
33	22.49384621991768	27.947711517118695	28.099154406196952	21.45928785676667
34	21.838968741735627	27.48682785916263	29.135464534109683	21.538738864992055
35	21.25591361878434	28.135037329541984	28.19787482680132	22.411174224872347
36	22.558831041723646	28.39378993741114	27.74388521081076	21.30349381005445
37	22.325224734466367	28.941018645558092	28.16357414259591	20.570182477379632
38	21.547949757126293	27.584757160197192	27.968450151555906	22.89884293112061
39	21.94192730289256	26.64083849633034	28.56958800753146	22.847646193245648
40	21.532579434055663	27.72574596924579	29.923419625642218	20.818254971056323
41	20.740499219051966	28.741882548128324	28.84964433524483	21.66797389757488
42	22.385180296149958	28.10836529833119	27.828026427914317	21.678427977604535
43	23.013442251661917	26.755550833953045	28.430237946163185	21.800768968221856
44	21.601237311007186	27.11986139585137	27.93386692464699	23.345034368494456
45	21.288688572390832	26.542061567185264	28.279925227898943	23.88932463252496
46	21.879993942284436	27.69670057932556	27.676357504673256	22.746947973716747
47	20.980830042652645	27.414440418524855	29.465926480128207	22.138803058694293
48	21.223082156637155	26.074001324560193	29.23328081806284	23.46963570073981
49	21.636046572078904	25.563446659328093	29.77361548424429	23.026891284348714
50	20.427441903569303	25.453763581827758	30.298975387140015	23.819819127462925
51	20.072398742345918	25.70177956696375	28.369152213665572	25.856669477024756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	385.0
1	792.0
2	1199.0
3	8042.0
4	14885.0
5	10341.0
6	5797.0
7	5649.0
8	5501.0
9	5448.5
10	5396.0
11	5436.0
12	5476.0
13	5186.0
14	4896.0
15	4808.0
16	4720.0
17	4778.5
18	4837.0
19	5305.5
20	5774.0
21	6205.5
22	6637.0
23	7819.5
24	9002.0
25	11019.0
26	15950.0
27	18864.0
28	23145.0
29	27426.0
30	30894.0
31	34362.0
32	39994.5
33	45627.0
34	52339.5
35	59052.0
36	62945.5
37	66839.0
38	73071.0
39	79303.0
40	85099.0
41	90895.0
42	100411.5
43	109928.0
44	118369.0
45	126810.0
46	142753.5
47	158697.0
48	166172.0
49	173647.0
50	168037.5
51	162428.0
52	143954.0
53	125480.0
54	111327.0
55	97174.0
56	86282.5
57	75391.0
58	69981.0
59	64571.0
60	61020.0
61	57469.0
62	49775.0
63	42081.0
64	35308.0
65	28535.0
66	22173.0
67	15811.0
68	12611.0
69	9411.0
70	7588.0
71	5765.0
72	4393.0
73	3021.0
74	2318.5
75	1270.5
76	925.0
77	703.0
78	481.0
79	410.5
80	340.0
81	213.5
82	87.0
83	59.0
84	31.0
85	29.0
86	27.0
87	16.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1769644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.063215727254672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12276127116434	19.058143015828993
2	10.469929571168954	5.4576006612507895
3	3.8350627897485547	2.998622064503511
4	1.911685125865	1.99298647352014
5	1.1465181916652614	1.4940975482296812
6	0.8231567514364012	1.2872467194019848
7	0.6303233951527231	1.149977823806071
8	0.5319543436078995	1.1091552651602274
9	0.39967244976196914	0.9375074350547902
>10	5.703141717141228	36.00184170264305
>50	1.344336110441141	23.061045834080172
>100	0.07526037214849597	2.98590576963361
>500	0.0030989553490436967	0.5672850271667265
>1k	0.002877601395540575	1.416106811434401
>5k	2.213539535031212E-4	0.4824778482859079
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8363	0.4725809258811377	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	3161	0.17862349715536005	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTG	2843	0.16065378121249246	No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	2784	0.1573197773111428	No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT	2302	0.1300826606933372	No Hit
GTTGAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC	2189	0.12369719559414209	No Hit
GTTGAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATG	1958	0.11064372269224772	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT	1783	0.10075472806960044	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6508540700841524E-5	0.0	0.0	0.13725924536234407	0.0
2	5.6508540700841524E-5	0.0	0.0	0.6214244220871542	0.0
3	5.6508540700841524E-5	0.0	0.0	1.0464251566981833	0.0
4	5.6508540700841524E-5	0.0	0.0	1.429496554109188	0.0
5	5.6508540700841524E-5	0.0	0.0	2.4997683149831267	0.0
6	5.6508540700841524E-5	0.0	0.0	3.2392955871350395	0.0
7	5.6508540700841524E-5	0.0	0.0	3.91152118731225	0.0
8	5.6508540700841524E-5	0.0	0.0	4.999480121425552	0.0
9	5.6508540700841524E-5	0.0	0.0	5.382608027377258	0.0
10	5.6508540700841524E-5	0.0	0.0	6.218143310179901	0.0
11	5.6508540700841524E-5	0.0	0.0	7.328818677654941	0.0
12	5.6508540700841524E-5	0.0	0.0	8.235385196118541	0.0
13	5.6508540700841524E-5	0.0	0.0	8.714182061476771	0.0
14	5.6508540700841524E-5	0.0	0.0	8.87121929608441	0.0
15	5.6508540700841524E-5	0.0	0.0	9.209535929260348	0.0
16	5.6508540700841524E-5	0.0	0.0	9.769196516361482	0.0
17	1.6952562210252457E-4	0.0	0.0	10.492166786088049	0.0
18	1.6952562210252457E-4	0.0	0.0	11.253845406194692	0.0
19	1.6952562210252457E-4	0.0	0.0	11.753437414530833	0.0
20	1.6952562210252457E-4	0.0	0.0	12.20454509494565	0.0
21	1.6952562210252457E-4	0.0	0.0	12.771099724012288	0.0
22	1.6952562210252457E-4	0.0	0.0	13.460334394940451	0.0
23	1.6952562210252457E-4	0.0	0.0	14.108487356779104	0.0
24	1.6952562210252457E-4	0.0	0.0	14.590222666253778	0.0
25	1.6952562210252457E-4	0.0	0.0	15.042799568726817	0.0
26	1.6952562210252457E-4	0.0	0.0	15.451469335075304	0.0
27	1.6952562210252457E-4	0.0	0.0	15.877882783203853	0.0
28	1.6952562210252457E-4	0.0	0.0	16.29197736945962	0.0
29	1.6952562210252457E-4	0.0	0.0	16.74376315236285	0.0
30	1.6952562210252457E-4	0.0	0.0	17.262172504752368	0.0
31	2.260341628033661E-4	0.0	0.0	17.754531419878802	0.0
32	2.260341628033661E-4	0.0	0.0	18.213945855776643	0.0
33	2.260341628033661E-4	0.0	0.0	18.649739721661533	0.0
34	2.260341628033661E-4	0.0	0.0	19.079826224935637	0.0
35	2.260341628033661E-4	0.0	0.0	19.542009579327818	0.0
36	2.260341628033661E-4	0.0	0.0	20.005210087452618	0.0
37	2.260341628033661E-4	0.0	0.0	20.474287483810304	0.0
38	2.260341628033661E-4	0.0	0.0	20.925903741091428	0.0
39	2.260341628033661E-4	0.0	0.0	21.37056944786635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGTA	20	7.0343213E-4	45.0	31
CGTTTTT	5675	0.0	42.541847	1
TTTCGCG	75	0.0	42.0	1
GTTAACG	55	6.184564E-11	40.909092	1
GCTATCG	40	3.459627E-7	39.375	1
ATGCGAT	40	3.459627E-7	39.375	12
TCGAATC	35	6.250202E-6	38.571426	2
TTAACGG	180	0.0	37.500004	2
AGGGCGA	1475	0.0	37.067795	6
GGATCGA	195	0.0	36.923077	8
GCGTTAG	165	0.0	36.81818	1
GTTGAAT	1830	0.0	36.270493	1
TAAGGGA	2010	0.0	36.044777	4
GACCGGT	25	0.0021074149	36.000004	4
CGTTCGC	25	0.0021074149	36.000004	17
TCCCGTT	25	0.0021074149	36.000004	30
TATCGAG	25	0.0021074149	36.000004	1
GGACCGA	900	0.0	36.0	8
CGTAAGG	295	0.0	35.847458	2
TGCGAAG	170	0.0	35.735294	1
>>END_MODULE