##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934844.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187439
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32518049348219	33.0	31.0	34.0	30.0	34.0
2	32.4934030295451	34.0	31.0	34.0	31.0	34.0
3	32.4992037485715	34.0	31.0	34.0	31.0	34.0
4	36.016286310286254	37.0	35.0	37.0	35.0	37.0
5	35.92131890564484	37.0	35.0	37.0	35.0	37.0
6	35.86098233256614	37.0	35.0	37.0	35.0	37.0
7	36.14685807018297	37.0	35.0	37.0	35.0	37.0
8	35.96225237675367	37.0	35.0	37.0	35.0	37.0
9	37.63262028617891	39.0	38.0	39.0	35.0	39.0
10	37.41727027662053	39.0	37.0	39.0	35.0	39.0
11	37.30363580781834	39.0	37.0	39.0	34.0	39.0
12	37.20567540732619	39.0	37.0	39.0	34.0	39.0
13	37.168788459870356	39.0	37.0	39.0	34.0	39.0
14	38.35737751581345	40.0	38.0	41.0	34.0	41.0
15	38.439165296069945	40.0	38.0	41.0	34.0	41.0
16	38.52669652925329	40.0	38.0	41.0	34.0	41.0
17	38.50550049307796	40.0	38.0	41.0	34.0	41.0
18	38.462316801115676	40.0	38.0	41.0	34.0	41.0
19	38.45957813411889	40.0	37.0	41.0	34.0	41.0
20	38.39562874387653	40.0	37.0	41.0	34.0	41.0
21	38.33621095483642	40.0	37.0	41.0	34.0	41.0
22	38.2749033845107	40.0	37.0	41.0	34.0	41.0
23	38.12290736618892	40.0	37.0	41.0	34.0	41.0
24	37.897091976935236	40.0	36.0	41.0	33.0	41.0
25	37.81359968806819	40.0	36.0	41.0	33.0	41.0
26	37.93525646370045	40.0	36.0	41.0	34.0	41.0
27	37.99953092327269	40.0	36.0	41.0	34.0	41.0
28	37.958354913389236	40.0	36.0	41.0	34.0	41.0
29	37.93816608684741	40.0	36.0	41.0	34.0	41.0
30	37.852053873925314	40.0	36.0	41.0	34.0	41.0
31	37.734265086459175	40.0	36.0	41.0	33.0	41.0
32	37.62953802258474	40.0	36.0	41.0	33.0	41.0
33	37.54413995161015	40.0	36.0	41.0	33.0	41.0
34	37.443192450306924	40.0	36.0	41.0	33.0	41.0
35	37.35647052185418	40.0	35.0	41.0	33.0	41.0
36	37.30448469352952	40.0	35.0	41.0	33.0	41.0
37	37.19303054725337	39.0	35.0	41.0	32.0	41.0
38	37.11770962550497	39.0	35.0	41.0	32.0	41.0
39	37.16892320363404	39.0	35.0	41.0	32.0	41.0
40	37.079060903339034	39.0	35.0	41.0	32.0	41.0
41	37.03069884010884	39.0	35.0	41.0	32.0	41.0
42	36.901372617877634	39.0	35.0	41.0	31.0	41.0
43	36.829505347222046	39.0	35.0	41.0	31.0	41.0
44	36.76957300543438	39.0	35.0	41.0	31.0	41.0
45	36.75012190099871	39.0	35.0	41.0	31.0	41.0
46	36.72670511916823	39.0	35.0	41.0	31.0	41.0
47	36.60445547097577	39.0	35.0	41.0	31.0	41.0
48	36.54211290011529	39.0	35.0	41.0	31.0	41.0
49	36.503689873753515	39.0	35.0	41.0	31.0	41.0
50	36.40791569082707	39.0	35.0	41.0	31.0	41.0
51	35.4896495735781	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	26.0
11	24.0
12	9.0
13	16.0
14	21.0
15	32.0
16	61.0
17	136.0
18	229.0
19	396.0
20	773.0
21	1188.0
22	1847.0
23	2605.0
24	3779.0
25	5384.0
26	7710.0
27	9837.0
28	11185.0
29	12852.0
30	15587.0
31	19449.0
32	25775.0
33	36187.0
34	68750.0
35	94678.0
36	84908.0
37	134487.0
38	245444.0
39	403670.0
40	372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22039111061705	21.127653715264533	25.482151083129324	12.169804090989095
2	31.76449484984071	24.513343422272637	26.22736831113009	17.494793416756565
3	31.86454209437285	23.804759655022277	27.59282792631874	16.73787032428613
4	28.686105138874503	26.404303715811928	26.47167559765175	18.437915547661817
5	25.619505507230265	30.001204272387888	25.049539386865344	19.329750833516503
6	27.055031879532336	33.86944508307374	23.350757386274157	15.724765651119762
7	82.2854900335933	5.515230677112677	7.871478029608257	4.327801259685761
8	82.87044639766759	4.73253783983851	7.401222294366279	4.995793468127626
9	77.0545686978447	7.162641617800998	9.897771590793296	5.885018093561016
10	39.347621225174514	32.07752145583899	16.085289433815124	12.489567885171365
11	27.56579495873051	24.55932473162832	28.364488617941635	19.51039169169953
12	26.897886965140945	22.231205139800867	31.50511310475738	19.365794790300807
13	23.556073196181025	24.74737649681373	31.891153987699578	19.805396319305665
14	19.058831653668104	27.973815918122952	30.842173787453504	22.12517864075544
15	17.767902182764754	27.05865311818123	35.1186882020887	20.054756496965318
16	20.81117430032195	25.747427867873636	32.44065589895565	21.00074193284876
17	20.525938595582595	26.15553304211837	28.8403867482877	24.47814161401133
18	20.98221466534281	26.980670164951633	31.142736595311423	20.89437857439414
19	23.888216573651363	26.37381793927941	28.344698127651192	21.39326735941804
20	25.035728151088183	26.38990297606866	29.11762204205858	19.45674683078457
21	23.117482245403764	26.524646739748313	30.324926164628245	20.032944850219675
22	21.750506762873716	24.894499843781446	28.57788905366928	24.777104339675553
23	20.957202854209775	26.199661624723458	29.807931186359887	23.035204334706876
24	21.595804079199016	25.4520863808583	30.487460829566825	22.464648710375858
25	20.81210066369725	27.021514368317025	28.415943892696806	23.75044107528892
26	19.791164009267	29.94612775898383	27.781300765765653	22.481407465983516
27	21.460891885814767	28.27572616361767	28.930328210543866	21.333053740023697
28	18.747489344715813	27.357026339879354	32.00551775712268	21.88996655828215
29	20.337886830397185	26.807271784066383	30.020236829007636	22.8346045565288
30	22.857847855763538	26.380302482906494	28.71844364215762	22.043406019172355
31	22.6798176580018	28.392953238018965	27.073138072776793	21.854091031202444
32	23.029225080193594	29.848438530316084	25.82178958245434	21.300546807035982
33	23.293828146119505	27.401744426450534	26.854600531058857	22.449826896371096
34	20.89387328528034	26.988417931363212	29.019090664867836	23.098618118488613
35	21.917504814984177	27.727908549407594	27.036504612026384	23.318082023581844
36	23.689722166780776	27.82913480187193	26.854347886501962	21.626795144845335
37	23.194875694667264	27.88859048759557	28.823375348123147	20.093158469614018
38	20.39245805468744	29.05597677017514	27.105055501798407	23.44650967333901
39	22.908124122586507	26.443968911245125	27.3390885763395	23.30881838982887
40	23.220392794914098	25.711720770498523	28.769562057503585	22.29832437708379
41	21.250270540213013	27.080380550074572	27.524866540512814	24.144482369199597
42	22.974401211346436	27.38557517480898	26.764490639098092	22.87553297474649
43	23.182327681674593	26.195956171222267	27.865094543803938	22.7566216032992
44	21.299452013956085	26.133552965668134	27.764626225010296	24.80236879536549
45	20.941370461977414	26.077297444331876	27.19701812050977	25.78431397318094
46	22.435931445741634	26.65433761228998	27.66365261710286	23.24607832486553
47	20.862208500815623	26.389734546364064	30.001456916944786	22.746600035875527
48	21.268545163162063	24.868982743534616	29.866544723560533	23.995927369742784
49	21.638332579610406	24.642781650257405	31.11974594063358	22.59913982949861
50	20.274894120876947	24.654487514727073	29.620300495435973	25.450317868960003
51	19.867125806041404	24.46180393266517	27.65548377642978	28.01558648486364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	283.0
1	723.0
2	1163.0
3	4308.5
4	7454.0
5	5147.0
6	2840.0
7	2684.5
8	2529.0
9	2503.5
10	2478.0
11	2367.5
12	2257.0
13	2244.0
14	2231.0
15	2267.5
16	2304.0
17	2343.0
18	2382.0
19	2787.5
20	3193.0
21	3330.0
22	3467.0
23	4177.5
24	4888.0
25	6297.0
26	9219.5
27	10733.0
28	13607.0
29	16481.0
30	19308.5
31	22136.0
32	25310.5
33	28485.0
34	32661.5
35	36838.0
36	38540.0
37	40242.0
38	43442.0
39	46642.0
40	53425.0
41	60208.0
42	66685.0
43	73162.0
44	80100.5
45	87039.0
46	98143.0
47	109247.0
48	115682.0
49	122117.0
50	118558.5
51	115000.0
52	99842.0
53	84684.0
54	71838.0
55	58992.0
56	53509.0
57	48026.0
58	45063.0
59	42100.0
60	40974.5
61	39849.0
62	36298.0
63	32747.0
64	29110.0
65	25473.0
66	20781.5
67	16090.0
68	13312.5
69	10535.0
70	8594.5
71	6654.0
72	5348.0
73	4042.0
74	3214.0
75	1931.0
76	1476.0
77	1007.5
78	539.0
79	369.0
80	199.0
81	135.5
82	72.0
83	49.5
84	27.0
85	21.0
86	15.0
87	13.5
88	12.0
89	10.5
90	9.0
91	7.5
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1187439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.116900814257978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0786255023752	15.27805108621304
2	12.237437808000927	5.413083964404142
3	5.300031387689034	3.516608055419174
4	2.7995754971577522	2.476717343706599
5	1.6901989584607595	1.8690981360319376
6	1.0912278467208931	1.4480746831009381
7	0.8010822227352606	1.2402219245010753
8	0.5302911962968597	0.9382718232937484
9	0.42886088582509097	0.8536566307417517
>10	3.626061143906883	19.067195368910916
>50	1.8587807244617498	29.968125839765953
>100	0.5501220359501253	15.513429211935875
>500	0.004622874251681684	0.7289120307350252
>1k	0.00308191616778779	1.6885539012398283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	4552	0.38334600766860444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4001	0.336943624051425	No Hit
CCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	3540	0.29812057714122575	No Hit
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT	2752	0.2317592735289981	No Hit
TCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	1776	0.1495655776844116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5264455689934386E-4	0.0	0.0	0.24540207960156268	0.0
2	2.5264455689934386E-4	0.0	0.0	1.2357687426469908	0.0
3	2.5264455689934386E-4	0.0	0.0	1.75587967045044	0.0
4	2.5264455689934386E-4	0.0	0.0	2.3252562868492612	0.0
5	2.5264455689934386E-4	0.0	0.0	3.815185453736992	0.0
6	2.5264455689934386E-4	0.0	0.0	4.561750119374553	0.0
7	2.5264455689934386E-4	0.0	0.0	5.3372004793509396	0.0
8	2.5264455689934386E-4	0.0	0.0	6.546020469262	0.0
9	2.5264455689934386E-4	0.0	0.0	6.964231425782714	0.0
10	2.5264455689934386E-4	0.0	0.0	7.934133879719295	0.0
11	2.5264455689934386E-4	0.0	0.0	9.465244109381619	0.0
12	2.5264455689934386E-4	0.0	0.0	10.692675581650931	0.0
13	3.3685940919912517E-4	0.0	0.0	11.171942306088987	0.0
14	3.3685940919912517E-4	0.0	0.0	11.341972092882244	0.0
15	3.3685940919912517E-4	0.0	0.0	11.685316045708452	0.0
16	3.3685940919912517E-4	0.0	0.0	12.424217159786734	0.0
17	3.3685940919912517E-4	0.0	0.0	13.368097224362684	0.0
18	3.3685940919912517E-4	0.0	0.0	14.35526372302072	0.0
19	3.3685940919912517E-4	0.0	0.0	14.989233131133473	0.0
20	3.3685940919912517E-4	0.0	0.0	15.568968174365168	0.0
21	3.3685940919912517E-4	0.0	0.0	16.309806230046345	0.0
22	3.3685940919912517E-4	0.0	0.0	17.117931952715043	0.0
23	3.3685940919912517E-4	0.0	0.0	17.877381490754473	0.0
24	3.3685940919912517E-4	0.0	0.0	18.476233305458216	0.0
25	3.3685940919912517E-4	0.0	0.0	18.986491095542593	0.0
26	3.3685940919912517E-4	0.0	0.0	19.47308451213073	0.0
27	3.3685940919912517E-4	0.0	0.0	19.9458666929417	0.0
28	3.3685940919912517E-4	0.0	0.0	20.433133828348236	0.0
29	3.3685940919912517E-4	0.0	0.0	20.954592193788482	0.0
30	3.3685940919912517E-4	0.0	0.0	21.538959053896665	0.0
31	3.3685940919912517E-4	0.0	0.0	22.08340807401475	0.0
32	4.210742614989065E-4	0.0	0.0	22.597960821566414	0.0
33	4.210742614989065E-4	0.0	0.0	23.10021820068231	0.0
34	4.210742614989065E-4	0.0	0.0	23.594306739125127	0.0
35	4.210742614989065E-4	0.0	0.0	24.126039316545945	0.0
36	4.210742614989065E-4	0.0	0.0	24.653055862237977	0.0
37	4.210742614989065E-4	0.0	0.0	25.161629355276354	0.0
38	4.210742614989065E-4	0.0	0.0	25.676771606794116	0.0
39	4.210742614989065E-4	0.0	0.0	26.177849977977814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAA	35	1.2119744E-7	45.000004	31
TAACGCG	30	2.1655633E-6	45.000004	1
ATCGCAA	35	1.2119744E-7	45.000004	30
CTTACCG	35	1.2119744E-7	45.000004	1
CGACGTC	20	7.0333807E-4	45.0	27
ATCCGAT	20	7.0333807E-4	45.0	32
CCCGTAA	20	7.0333807E-4	45.0	17
CGGATAT	20	7.0333807E-4	45.0	34
AATTCGC	20	7.0333807E-4	45.0	13
CCGACGT	20	7.0333807E-4	45.0	25
ACGTCAC	110	0.0	45.0	20
GCGGATA	20	7.0333807E-4	45.0	33
TCGCGTG	20	7.0333807E-4	45.0	45
TCGAACG	20	7.0333807E-4	45.0	1
CTATCGC	25	3.8908853E-5	44.999996	12
GTAATAC	25	3.8908853E-5	44.999996	23
CCGTACA	25	3.8908853E-5	44.999996	41
GAACGAT	25	3.8908853E-5	44.999996	27
ACGTCGT	25	3.8908853E-5	44.999996	42
ACCGTAC	25	3.8908853E-5	44.999996	40
>>END_MODULE