##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934843.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535823
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19820351123412	33.0	31.0	34.0	30.0	34.0
2	32.37340502367386	34.0	31.0	34.0	30.0	34.0
3	32.38282977774377	34.0	31.0	34.0	30.0	34.0
4	35.924159657200235	37.0	35.0	37.0	35.0	37.0
5	35.820791567364594	37.0	35.0	37.0	35.0	37.0
6	35.76012041289754	37.0	35.0	37.0	35.0	37.0
7	36.08616838023004	37.0	35.0	37.0	35.0	37.0
8	35.92510213260722	37.0	35.0	37.0	35.0	37.0
9	37.58809718881049	39.0	38.0	39.0	35.0	39.0
10	37.33801273928144	39.0	37.0	39.0	34.0	39.0
11	37.1776183553151	39.0	37.0	39.0	34.0	39.0
12	37.05412048381648	39.0	37.0	39.0	34.0	39.0
13	36.91155661477764	39.0	37.0	39.0	33.0	39.0
14	38.01922649830261	40.0	37.0	41.0	33.0	41.0
15	38.134152509317445	40.0	37.0	41.0	33.0	41.0
16	38.229611270886096	40.0	37.0	41.0	34.0	41.0
17	38.23553860136649	40.0	37.0	41.0	34.0	41.0
18	38.19174615498028	40.0	37.0	41.0	34.0	41.0
19	38.17960035310168	40.0	37.0	41.0	34.0	41.0
20	38.122682303671176	40.0	36.0	41.0	34.0	41.0
21	38.039132325413426	40.0	36.0	41.0	34.0	41.0
22	37.95574658049393	40.0	36.0	41.0	34.0	41.0
23	37.80258219598636	40.0	35.0	41.0	33.0	41.0
24	37.56726381659615	39.0	35.0	41.0	33.0	41.0
25	37.477444977165966	39.0	35.0	41.0	33.0	41.0
26	37.64866569744113	39.0	35.0	41.0	33.0	41.0
27	37.72040767193644	39.0	35.0	41.0	33.0	41.0
28	37.681458242740604	40.0	36.0	41.0	33.0	41.0
29	37.70851381892901	40.0	36.0	41.0	33.0	41.0
30	37.539749133575825	40.0	36.0	41.0	33.0	41.0
31	37.41490380218841	39.0	35.0	41.0	33.0	41.0
32	37.34846021913953	39.0	35.0	41.0	33.0	41.0
33	37.16335618291861	39.0	35.0	41.0	32.0	41.0
34	37.09390041114323	39.0	35.0	41.0	32.0	41.0
35	37.071236210465024	39.0	35.0	41.0	32.0	41.0
36	36.965061223575695	39.0	35.0	41.0	32.0	41.0
37	36.92094964195266	39.0	35.0	41.0	32.0	41.0
38	36.85114114175763	39.0	35.0	41.0	31.0	41.0
39	36.843672257443224	39.0	35.0	41.0	31.0	41.0
40	36.754698846447425	39.0	35.0	41.0	31.0	41.0
41	36.76580512594644	39.0	35.0	41.0	31.0	41.0
42	36.71020467579779	39.0	35.0	41.0	31.0	41.0
43	36.669099684037455	39.0	35.0	41.0	31.0	41.0
44	36.56552256995314	39.0	35.0	41.0	31.0	41.0
45	36.48963743624294	39.0	35.0	41.0	31.0	41.0
46	36.41477876089679	39.0	35.0	41.0	31.0	41.0
47	36.309471597897065	39.0	35.0	41.0	31.0	41.0
48	36.3074093497293	39.0	35.0	41.0	31.0	41.0
49	36.30841341263813	39.0	35.0	40.0	31.0	41.0
50	36.185766568437714	39.0	35.0	40.0	31.0	41.0
51	35.27345970591035	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	8.0
10	3.0
11	6.0
12	5.0
13	5.0
14	13.0
15	16.0
16	27.0
17	71.0
18	117.0
19	248.0
20	421.0
21	649.0
22	1006.0
23	1481.0
24	2114.0
25	3112.0
26	4212.0
27	4935.0
28	5456.0
29	6239.0
30	7475.0
31	9556.0
32	12487.0
33	17876.0
34	34489.0
35	50056.0
36	39048.0
37	60016.0
38	109185.0
39	165368.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18117923269438	22.094236342971467	25.451128450999676	12.273455973334478
2	31.42716904649483	24.542992742006223	26.19764362485373	17.832194586645215
3	30.347708105101873	23.983666247996073	28.710600328839934	16.95802531806212
4	27.73807768610157	25.473337277421837	27.130600963377834	19.657984073098767
5	24.125130873441417	30.060299763168064	26.12803108489184	19.686538278498684
6	26.122432221087934	32.111723460918995	26.26725616481562	15.498588153177447
7	80.56839665337247	5.454226488971171	9.104499060324025	4.872877797332328
8	80.6536860119853	4.338186304059363	8.88800965990635	6.1201180240489865
9	75.1300336118457	7.160573547608072	11.383983143687376	6.325409696858851
10	40.17875305837935	30.76986243591634	17.37532730024654	11.676057205457772
11	26.916724366068646	23.712494611093586	30.720965692028894	18.649815330808867
12	27.074052438958386	21.7846191746155	32.27427714002572	18.867051246400397
13	23.72201267956023	26.493077004906475	32.15688762893717	17.628022686596132
14	16.730524818830098	31.414664917332775	30.236663972991078	21.618146290846045
15	16.51011621374969	27.92172041886967	37.091912814492844	18.4762505528878
16	18.65205487633043	26.14184161560814	35.441554393895	19.76454911416643
17	18.413916535870985	25.047263741944636	30.333710945592106	26.20510877659227
18	19.19607780927657	25.721553572728308	32.47732926731402	22.605039350681103
19	21.153253966328435	27.839417120952252	29.763559981561077	21.243768931158236
20	24.632761191662173	26.85718978095378	29.254063375405686	19.255985651978357
21	22.652629693014298	26.445300033779812	30.807748080989434	20.09432219221646
22	19.609647215591718	25.146736888860687	29.973517374207525	25.270098521340067
23	19.31234754760434	26.728229284670498	30.108823249468575	23.850599918256588
24	22.39881453390392	24.40843338191903	30.91449975085056	22.27825233332649
25	18.409997331208253	27.067520431187162	29.286350156674875	25.236132080929707
26	19.075888866286068	28.249067322604667	29.10849291650414	23.566550894605122
27	20.67081853522525	29.785582179189767	28.7992490057351	20.744350279849876
28	17.906472846443695	28.22704512497597	30.911140432568217	22.955341596012115
29	19.47172853722218	26.07540176513513	30.447554509604853	24.00531518803784
30	22.507245862906224	27.65745404732533	28.641547675258433	21.193752414510016
31	21.21577461213871	30.47424242707013	25.04222476452112	23.267758196270037
32	22.78812219706881	29.53643273991598	24.55176429529901	23.123680767716202
33	24.078473675075536	28.835454991667024	26.115153698142855	20.97091763511458
34	20.049904539372143	29.607351681432114	26.83722050005319	23.505523279142555
35	21.284454008133284	26.07372210599396	26.53450859705537	26.10731528881739
36	22.025183689389966	27.10596596264065	28.613926613825836	22.25492373414355
37	21.01645506071968	29.414750766577768	28.71321313194842	20.855581040754128
38	20.308572047112573	29.683682857958694	26.65544405521973	23.35230103970901
39	21.96583573306857	27.500125974435583	29.109612689264925	21.42442560323092
40	21.78275288668086	26.512859657013603	29.23465398088548	22.469733475420053
41	19.198317354798135	26.288345218476998	28.09995091662732	26.413386510097553
42	21.273816166905863	25.407457313329214	28.468729412511223	24.849997107253703
43	24.65347698773662	24.746044869294526	27.71008336708204	22.89039477588681
44	20.937884338671537	27.37079222056537	27.150196986691498	24.541126454071588
45	21.796936674984092	26.969167057031896	25.877201986476877	25.356694281507142
46	22.588242759269388	27.133960281660173	26.789630157719994	23.488166801350445
47	18.90866946734276	27.75953999734987	29.5215024364389	23.81028809886847
48	20.692840732853945	23.856945297234347	29.768598958984587	25.681615010927118
49	21.306102948175052	22.727094581606238	31.86425368078638	24.102548789432333
50	20.05867609266493	23.72854468733145	30.261112344934798	25.951666875068817
51	18.717001696455732	24.364388986661638	26.99286891380176	29.92574040308087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	259.0
1	328.5
2	398.0
3	1908.5
4	3419.0
5	2305.0
6	1191.0
7	1243.5
8	1296.0
9	1304.5
10	1313.0
11	1393.0
12	1473.0
13	1455.0
14	1437.0
15	1345.5
16	1254.0
17	1289.5
18	1325.0
19	1556.0
20	1787.0
21	1882.5
22	1978.0
23	2111.5
24	2245.0
25	3052.0
26	4329.0
27	4799.0
28	5588.0
29	6377.0
30	7354.0
31	8331.0
32	10216.5
33	12102.0
34	13461.0
35	14820.0
36	15906.0
37	16992.0
38	18059.0
39	19126.0
40	23277.0
41	27428.0
42	30350.0
43	33272.0
44	37332.0
45	41392.0
46	45909.5
47	50427.0
48	57190.5
49	63954.0
50	62118.0
51	60282.0
52	51444.0
53	42606.0
54	35377.5
55	28149.0
56	25026.0
57	21903.0
58	20035.5
59	18168.0
60	16360.5
61	14553.0
62	13287.0
63	12021.0
64	9528.0
65	7035.0
66	5322.0
67	3609.0
68	2991.5
69	2374.0
70	1828.5
71	1283.0
72	1042.5
73	802.0
74	620.0
75	287.0
76	136.0
77	122.5
78	109.0
79	74.5
80	40.0
81	37.5
82	35.0
83	25.5
84	16.0
85	9.5
86	3.0
87	4.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	535823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.02299516509677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.92527318960828	18.196650461312466
2	12.125820409005412	6.311003317527591
3	5.286561716249205	4.1271650994581615
4	2.802937749582229	2.9176334202178227
5	1.7275764184008655	2.247835639169046
6	1.113662446546533	1.738849947721816
7	0.7426955082568918	1.3529013134355727
8	0.5047049424229021	1.0507147421177299
9	0.3876498814131874	0.9079029890789139
>10	3.629172650013158	22.560239490513627
>50	1.5208075314701164	28.238870605128625
>100	0.22591967600861246	7.713838701170814
>500	0.0036089405113196873	0.6078207372404001
>1k	0.0036089405113196873	2.0285735359073924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	3248	0.6061703211694908	No Hit
CCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	2283	0.4260735354772005	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2079	0.3880012616106438	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	1937	0.3614999729388249	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	1253	0.23384587820978195	No Hit
ACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	950	0.17729735379033748	No Hit
CGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	661	0.1233616324793822	No Hit
AGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	560	0.10451212433956736	No Hit
ACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	546	0.10189932123107817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.732575869270263E-4	0.0	0.0	0.3878146328171803	0.0
2	3.732575869270263E-4	0.0	0.0	1.9299283532061893	0.0
3	3.732575869270263E-4	0.0	0.0	2.704437846079769	0.0
4	3.732575869270263E-4	0.0	0.0	3.5270975676669347	0.0
5	3.732575869270263E-4	0.0	0.0	5.723158580352094	0.0
6	3.732575869270263E-4	0.0	0.0	6.731327322641992	0.0
7	3.732575869270263E-4	0.0	0.0	7.838409325467552	0.0
8	3.732575869270263E-4	0.0	0.0	9.573123960710907	0.0
9	3.732575869270263E-4	0.0	0.0	10.196837388465967	0.0
10	3.732575869270263E-4	0.0	0.0	11.575837543367866	0.0
11	5.598863803905394E-4	0.0	0.0	13.671678893963119	0.0
12	5.598863803905394E-4	0.0	0.0	15.27985920723821	0.0
13	5.598863803905394E-4	0.0	0.0	15.89386793773317	0.0
14	5.598863803905394E-4	0.0	0.0	16.10961082297699	0.0
15	5.598863803905394E-4	0.0	0.0	16.53456458569341	0.0
16	5.598863803905394E-4	0.0	0.0	17.434675256567935	0.0
17	5.598863803905394E-4	0.0	0.0	18.680049195349955	0.0
18	5.598863803905394E-4	0.0	0.0	20.00623340170168	0.0
19	5.598863803905394E-4	0.0	0.0	20.826093691386895	0.0
20	5.598863803905394E-4	0.0	0.0	21.532483674646294	0.0
21	5.598863803905394E-4	0.0	0.0	22.409825632718267	0.0
22	5.598863803905394E-4	0.0	0.0	23.312362477907815	0.0
23	5.598863803905394E-4	0.0	0.0	24.201462049967994	0.0
24	5.598863803905394E-4	0.0	0.0	24.8541029407099	0.0
25	5.598863803905394E-4	0.0	0.0	25.428732995784056	0.0
26	5.598863803905394E-4	0.0	0.0	25.94382846574335	0.0
27	5.598863803905394E-4	0.0	0.0	26.47814670142939	0.0
28	5.598863803905394E-4	0.0	0.0	26.96636762512994	0.0
29	5.598863803905394E-4	0.0	0.0	27.534279043639412	0.0
30	5.598863803905394E-4	0.0	0.0	28.176655350740823	0.0
31	5.598863803905394E-4	0.0	0.0	28.810073475755985	0.0
32	5.598863803905394E-4	0.0	0.0	29.353349893528275	0.0
33	5.598863803905394E-4	0.0	0.0	29.869378507454886	0.0
34	5.598863803905394E-4	0.0	0.0	30.363198294959343	0.0
35	5.598863803905394E-4	0.0	0.0	30.871575128353953	0.0
36	5.598863803905394E-4	0.0	0.0	31.417464349234727	0.0
37	5.598863803905394E-4	0.0	0.0	32.0068380789925	0.0
38	5.598863803905394E-4	0.0	0.0	32.53910339795044	0.0
39	5.598863803905394E-4	0.0	0.0	33.05625178463784	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATA	35	1.210301E-7	45.000004	25
CAACGTT	35	1.210301E-7	45.000004	18
TCATTCG	30	2.163324E-6	45.000004	15
GCTATCC	35	1.210301E-7	45.000004	19
CGACCGG	30	2.163324E-6	45.000004	3
CCTACGG	30	2.163324E-6	45.000004	2
GTAATGC	35	1.210301E-7	45.000004	28
TCGGAGC	30	2.163324E-6	45.000004	15
TCGTGAC	30	2.163324E-6	45.000004	29
CGAGAGG	35	1.210301E-7	45.000004	2
TCGAGCG	35	1.210301E-7	45.000004	1
TCGACCG	30	2.163324E-6	45.000004	2
GCGAGAC	25	3.888009E-5	45.0	21
CTAGATG	45	3.8380676E-10	45.0	1
CGGGTAT	100	0.0	45.0	6
TTGGCGT	20	7.0299115E-4	45.0	45
GTCGCGC	20	7.0299115E-4	45.0	36
GGTACGC	20	7.0299115E-4	45.0	8
ACACGTC	20	7.0299115E-4	45.0	40
TACCCCG	20	7.0299115E-4	45.0	25
>>END_MODULE