##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934842.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360224
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25823931775784	33.0	31.0	34.0	30.0	34.0
2	32.45908934440792	34.0	31.0	34.0	31.0	34.0
3	32.4356650306476	34.0	31.0	34.0	30.0	34.0
4	35.96918028782091	37.0	35.0	37.0	35.0	37.0
5	35.864528737674334	37.0	35.0	37.0	35.0	37.0
6	35.79228202451808	37.0	35.0	37.0	35.0	37.0
7	36.06314126765568	37.0	35.0	37.0	35.0	37.0
8	35.8768349693524	37.0	35.0	37.0	35.0	37.0
9	37.539108998845165	39.0	38.0	39.0	35.0	39.0
10	37.34161799324865	39.0	37.0	39.0	34.0	39.0
11	37.190120591631874	39.0	37.0	39.0	34.0	39.0
12	37.00588245091943	39.0	35.0	39.0	33.0	39.0
13	36.8574581371591	39.0	35.0	39.0	33.0	39.0
14	37.95483365905659	40.0	37.0	41.0	33.0	41.0
15	38.03458958870036	40.0	37.0	41.0	33.0	41.0
16	38.190983943324156	40.0	37.0	41.0	34.0	41.0
17	38.14413809185396	40.0	36.0	41.0	34.0	41.0
18	38.08293173136715	40.0	36.0	41.0	34.0	41.0
19	37.99426467975482	40.0	36.0	41.0	34.0	41.0
20	37.86991705161233	40.0	35.0	41.0	34.0	41.0
21	37.80081282757396	40.0	35.0	41.0	34.0	41.0
22	37.73541185484587	39.0	35.0	41.0	33.0	41.0
23	37.55840254952474	39.0	35.0	41.0	33.0	41.0
24	37.29081904592698	39.0	35.0	41.0	33.0	41.0
25	37.23406547037399	39.0	35.0	41.0	33.0	41.0
26	37.38691480856356	39.0	35.0	41.0	33.0	41.0
27	37.426606778004796	39.0	35.0	41.0	33.0	41.0
28	37.36078106955672	39.0	35.0	41.0	33.0	41.0
29	37.36326285866572	39.0	35.0	41.0	33.0	41.0
30	37.21451097095141	39.0	35.0	41.0	33.0	41.0
31	37.08771764235587	39.0	35.0	41.0	32.0	41.0
32	36.89899340410411	39.0	35.0	41.0	32.0	41.0
33	36.69600581860176	39.0	35.0	41.0	31.0	41.0
34	36.55083781202807	39.0	35.0	41.0	31.0	41.0
35	36.43932664120103	39.0	35.0	41.0	31.0	41.0
36	36.341651305854136	39.0	35.0	41.0	30.0	41.0
37	36.26966276539042	39.0	35.0	41.0	30.0	41.0
38	36.15667195966954	39.0	35.0	41.0	30.0	41.0
39	36.17059940481478	39.0	35.0	41.0	30.0	41.0
40	36.093533468064315	39.0	35.0	41.0	30.0	41.0
41	35.9563021897486	39.0	35.0	41.0	30.0	41.0
42	35.86085879896953	38.0	35.0	40.0	29.0	41.0
43	35.79268455183441	38.0	35.0	40.0	29.0	41.0
44	35.75195711557253	38.0	35.0	40.0	29.0	41.0
45	35.69582537532202	38.0	35.0	40.0	29.0	41.0
46	35.620408412543306	38.0	35.0	40.0	29.0	41.0
47	35.44744381273874	38.0	35.0	40.0	28.0	41.0
48	35.41220740428178	38.0	35.0	40.0	28.0	41.0
49	35.39475437505552	38.0	35.0	40.0	28.0	41.0
50	35.29627676112641	38.0	35.0	40.0	27.0	41.0
51	34.26941014479879	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	10.0
11	8.0
12	11.0
13	12.0
14	11.0
15	23.0
16	27.0
17	54.0
18	118.0
19	202.0
20	384.0
21	640.0
22	997.0
23	1460.0
24	2100.0
25	3004.0
26	3988.0
27	4618.0
28	5039.0
29	4992.0
30	5748.0
31	6968.0
32	9210.0
33	12837.0
34	25231.0
35	34945.0
36	26759.0
37	39276.0
38	69830.0
39	101658.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.98739673092298	23.286066447543753	24.5158568002132	17.21068002132007
2	32.95310695567203	26.832193301945455	22.102636137514438	18.11206360486808
3	30.012436706049566	25.00360886559474	27.493170915874565	17.490783512481123
4	28.360964288886915	24.120269610020433	28.890357111130854	18.6284089899618
5	25.64848538687039	28.19412365639158	26.632595274051702	19.524795682686328
6	25.87889757484232	32.06976770009772	27.227502887092474	14.823831837967488
7	76.45825930532114	6.057064493204228	12.496113529359508	4.988562672115129
8	76.53654392822244	5.851636759349739	11.648030114595363	5.96378919783246
9	71.04829217375855	6.768011015368216	13.807797370525007	8.375899440348228
10	42.79614906280537	20.679632673003464	21.70010882117793	14.824109443013237
11	31.496235675579637	24.364006840188328	25.2431820200764	18.89657546415564
12	28.588045216309855	20.926701163720352	30.067957715199434	20.417295904770366
13	24.772086257439817	25.38559340854579	31.42794483432531	18.414375499689083
14	19.921770898107845	29.065803500044417	30.12542195966954	20.8870036421782
15	18.15398196677623	25.067458026117084	37.37313449409256	19.405425513014123
16	20.492249267122677	23.68609531846851	34.60346895265168	21.21818646175713
17	19.272452696100203	23.90318246424447	30.731988984631787	26.09237585502354
18	21.166274318202007	24.93254197388292	32.22467131562583	21.676512392289244
19	23.23776316958337	27.088977969263567	28.512814248911788	21.16044461224127
20	24.94447899085014	25.72316114417696	29.65543661721595	19.67692324775695
21	23.256917917740072	26.184540730212312	30.938527138669276	19.620014213378344
22	20.493082082259928	24.47171759793906	29.740661366260994	25.29453895354002
23	20.026150395309585	26.380252287465577	29.883905569867636	23.7096917473572
24	22.187028071422226	24.64105667584614	30.541551923247756	22.630363329483878
25	20.091387581060673	26.271431109531846	29.058308163809187	24.578873145598294
26	20.04474993337479	26.578462290130584	30.367771164608687	23.009016611885936
27	20.15051745580528	27.961490628053653	29.977458470285157	21.91053344585591
28	18.094852092031623	26.781669183619083	32.28102513991294	22.84245358443635
29	18.938771431109533	24.1533046104646	32.75128808741228	24.15663587101359
30	20.25489695300702	24.869248023452073	30.85052633916674	24.025328684374166
31	22.089866305409966	26.83025006662521	27.091198809629564	23.98868481833526
32	22.659511859287555	27.40711335169228	26.98820733765657	22.945167451363595
33	21.662076929910278	27.38074087234609	25.527171981877945	25.430010215865686
34	21.08465843475171	25.711224127209736	29.20849249355956	23.99562494447899
35	19.546171271209026	25.264835213644844	29.20988051878831	25.979112996357824
36	21.505785289153415	26.800268721684283	28.314604246246777	23.379341742915518
37	20.812327884871635	27.0745425068846	30.019376832193302	22.093752776050458
38	19.20471706493737	28.01645642711202	26.970718219774366	25.808108288176246
39	21.041907257706317	25.789231145065294	28.57721861952563	24.59164297770276
40	22.89936261881496	22.507661899262683	30.905769743270856	23.687205738651507
41	19.630840810162564	25.099937816469755	29.702907080039086	25.566314293328595
42	21.000544105889666	25.0	29.709847206182822	24.28960868792751
43	21.917473571999643	26.034911610553436	28.085024873412102	23.962589944034825
44	20.174946699831217	26.396075775073285	27.19696633206005	26.232011193035444
45	20.39869636670516	25.612674335968734	26.347494892067157	27.64113440525895
46	22.81830194545616	25.198210002665007	27.8024229368393	24.181065115039534
47	19.24635782179977	25.202651683396997	31.55675357555299	23.994236919250245
48	20.827318557342096	23.537854224038377	29.73455405525451	25.900273163365018
49	20.551101536821534	22.66867282579728	31.890434840543662	24.889790796837524
50	19.944812116905037	22.557353202451807	30.953795416185486	26.54403926445767
51	19.25773962867549	23.605312250155457	27.632528648840722	29.50441947232833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	403.0
1	570.0
2	737.0
3	2444.5
4	4152.0
5	2803.5
6	1455.0
7	1503.5
8	1552.0
9	1591.5
10	1631.0
11	1598.0
12	1565.0
13	1544.5
14	1524.0
15	1522.0
16	1520.0
17	1457.5
18	1395.0
19	1412.0
20	1429.0
21	1374.0
22	1319.0
23	1343.0
24	1367.0
25	1704.0
26	2218.5
27	2396.0
28	2461.0
29	2526.0
30	3310.5
31	4095.0
32	5102.5
33	6110.0
34	6825.0
35	7540.0
36	8225.5
37	8911.0
38	9819.0
39	10727.0
40	12067.0
41	13407.0
42	17121.5
43	20836.0
44	22658.5
45	24481.0
46	29209.5
47	33938.0
48	37060.5
49	40183.0
50	40341.0
51	40499.0
52	34535.5
53	28572.0
54	25044.0
55	21516.0
56	18997.5
57	16479.0
58	15169.0
59	13859.0
60	13295.0
61	12731.0
62	11466.0
63	10201.0
64	8725.5
65	7250.0
66	6153.0
67	5056.0
68	3850.5
69	2645.0
70	2300.0
71	1955.0
72	1524.5
73	1094.0
74	758.0
75	362.5
76	303.0
77	266.0
78	229.0
79	179.0
80	129.0
81	76.0
82	23.0
83	14.5
84	6.0
85	6.0
86	6.0
87	4.0
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	360224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.992207294771553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32269039258532	20.388100173878737
2	12.525887957553792	7.2630628043296435
3	4.748376063329873	4.129977094247729
4	2.292020555448877	2.6580294026980513
5	1.3617945916725556	1.9740715547334757
6	0.9041410778286076	1.5727827331275104
7	0.6060766626700552	1.2300050168457504
8	0.46751555461877575	1.0843446298430113
9	0.42664520976697096	1.1132447726596784
>10	5.561339975681716	39.60399875380992
>50	0.7137335401169604	13.228884661910318
>100	0.06080690774739468	2.746119239250925
>500	0.0049841727661798915	1.0794312101244163
>1k	0.003987338212943913	1.927947952540825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2408	0.6684729501643422	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	1649	0.4577707204406148	TruSeq Adapter, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	1562	0.4336190814604246	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCG	1052	0.29204050812827576	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGC	981	0.2723305498800746	No Hit
CTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGCT	896	0.24873412099138312	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTC	781	0.2168095407302123	No Hit
CGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTG	576	0.15990050635160344	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	501	0.13908012792040508	TruSeq Adapter, Index 14 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	394	0.10937638802522875	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2665008439193391	0.0
2	0.0	0.0	0.0	1.4865750199875634	0.0
3	0.0	0.0	0.0	2.1372812472239495	0.0
4	0.0	0.0	0.0	2.8287954161854847	0.0
5	0.0	0.0	0.0	4.803400106600337	0.0
6	0.0	0.0	0.0	5.720884782801813	0.0
7	0.0	0.0	0.0	6.739972905747535	0.0
8	0.0	0.0	0.0	8.290952296348939	0.0
9	0.0	0.0	0.0	8.836723816292086	0.0
10	0.0	0.0	0.0	10.167007195522785	0.0
11	0.0	0.0	0.0	11.957837345651594	0.0
12	0.0	0.0	0.0	13.674546948565338	0.0
13	0.0	0.0	0.0	14.22531535933197	0.0
14	0.0	0.0	0.0	14.451285866571911	0.0
15	0.0	0.0	0.0	14.896286754908058	0.0
16	0.0	0.0	0.0	15.771020254064139	0.0
17	0.0	0.0	0.0	16.77012081371591	0.0
18	0.0	0.0	0.0	17.861386248556453	0.0
19	0.0	0.0	0.0	18.594818779426134	0.0
20	0.0	0.0	0.0	19.256351603446745	0.0
21	0.0	0.0	0.0	20.02948165585858	0.0
22	0.0	0.0	0.0	20.820378431198364	0.0
23	0.0	0.0	0.0	21.642922181753576	0.0
24	0.0	0.0	0.0	22.270309585147018	0.0
25	0.0	0.0	0.0	22.787210180332238	0.0
26	0.0	0.0	0.0	23.267189304432797	0.0
27	0.0	0.0	0.0	23.754663764768587	0.0
28	0.0	0.0	0.0	24.252687216842855	0.0
29	0.0	0.0	0.0	24.76986541707382	0.0
30	0.0	0.0	0.0	25.328961979212934	0.0
31	0.0	0.0	0.0	25.85085946522164	0.0
32	0.0	0.0	0.0	26.361375144354625	0.0
33	0.0	0.0	0.0	26.85523452074265	0.0
34	0.0	0.0	0.0	27.35381318290841	0.0
35	0.0	0.0	0.0	27.88320600515235	0.0
36	0.0	0.0	0.0	28.37484454117438	0.0
37	0.0	0.0	0.0	28.881751354712623	0.0
38	0.0	0.0	0.0	29.372279470551657	0.0
39	0.0	0.0	0.0	29.885293595096385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	25	3.8854538E-5	45.000004	8
AGATCGC	30	2.161336E-6	45.000004	43
CTCGTCC	30	2.161336E-6	45.000004	37
CCGTGCT	30	2.161336E-6	45.000004	20
CCCGTAG	25	3.8854538E-5	45.000004	25
CGGATAG	25	3.8854538E-5	45.000004	10
TCTCGCG	25	3.8854538E-5	45.000004	32
CGTACTC	25	3.8854538E-5	45.000004	34
TATAGCG	25	3.8854538E-5	45.000004	1
GCGGATA	25	3.8854538E-5	45.000004	9
ATTGAGG	30	2.161336E-6	45.000004	2
GCCCGTA	25	3.8854538E-5	45.000004	24
CGCCCGT	25	3.8854538E-5	45.000004	23
AGGTCGC	30	2.161336E-6	45.000004	10
TTGATCG	45	3.8380676E-10	45.0	2
CTTCGCT	20	7.026831E-4	45.0	18
CCTTAGT	20	7.026831E-4	45.0	29
CGTATGG	20	7.026831E-4	45.0	2
CACGGCG	20	7.026831E-4	45.0	10
TTTCGCA	20	7.026831E-4	45.0	13
>>END_MODULE