FastQCFastQC Report
Sat 14 Jan 2017
SRR2934841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459506
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC99740.683381911413862TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC59160.4053426296294774TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT48090.3294950483245701TruSeq Adapter, Index 20 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42580.2917425485061384No Hit
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC37170.25467521202379434TruSeq Adapter, Index 23 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC26620.18239048006654304TruSeq Adapter, Index 20 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG19010.13024955019026985TruSeq Adapter, Index 20 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG18260.12511082516961217TruSeq Adapter, Index 20 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG17800.12195907382360882TruSeq Adapter, Index 20 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG15260.10455592508698149TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA207.0339127E-445.039
ATTCGTC207.0339127E-445.038
TTCGTCC207.0339127E-445.011
AGACGTC351.2122291E-745.016
CCGTAGA207.0339127E-445.017
GTTCGTC207.0339127E-445.010
CGTAGTT406.8175723E-945.022
CGTAGAT207.0339127E-445.018
CGCTAGA207.0339127E-445.010
CGCTACG207.0339127E-445.02
TGCGAAC207.0339127E-445.021
ACAATCG253.8913247E-545.01
AGCGAAT207.0339127E-445.010
AATTCGC207.0339127E-445.036
CGTACCA207.0339127E-445.017
ATGCGTA207.0339127E-445.015
CGTGTCG207.0339127E-445.02
ACGTACC207.0339127E-445.016
CGGTCCG351.2122291E-745.032
CGCCGGT253.8913247E-545.028
Produced by FastQC (version 0.11.5)