##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934838.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242531
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31130088504834	33.0	31.0	34.0	30.0	34.0
2	32.48824938774163	34.0	31.0	34.0	31.0	34.0
3	32.488771708713905	34.0	31.0	34.0	31.0	34.0
4	36.013691408906496	37.0	35.0	37.0	35.0	37.0
5	35.904292126313145	37.0	35.0	37.0	35.0	37.0
6	35.83256111919944	37.0	35.0	37.0	35.0	37.0
7	36.07225493770377	37.0	35.0	37.0	35.0	37.0
8	35.80149147184255	37.0	35.0	37.0	35.0	37.0
9	37.50530006897212	39.0	38.0	39.0	35.0	39.0
10	37.3592868105504	39.0	37.0	39.0	34.0	39.0
11	37.27154171606181	39.0	37.0	39.0	34.0	39.0
12	37.143674483775456	39.0	37.0	39.0	34.0	39.0
13	37.056111276096935	39.0	37.0	39.0	33.0	39.0
14	38.24316978811796	40.0	37.0	41.0	33.0	41.0
15	38.302285415816584	40.0	37.0	41.0	34.0	41.0
16	38.431385615328715	40.0	38.0	41.0	34.0	41.0
17	38.383949374301324	40.0	37.0	41.0	34.0	41.0
18	38.35093852789186	40.0	37.0	41.0	34.0	41.0
19	38.32428647655471	40.0	37.0	41.0	34.0	41.0
20	38.22036391848574	40.0	37.0	41.0	34.0	41.0
21	38.189083411198595	40.0	37.0	41.0	34.0	41.0
22	38.11095578299455	40.0	37.0	41.0	34.0	41.0
23	37.92833981606898	40.0	36.0	41.0	33.0	41.0
24	37.68913210213669	40.0	36.0	41.0	33.0	41.0
25	37.62406088862169	40.0	36.0	41.0	33.0	41.0
26	37.715872682452186	40.0	36.0	41.0	33.0	41.0
27	37.760275598757694	40.0	36.0	41.0	33.0	41.0
28	37.70316394520539	40.0	36.0	41.0	33.0	41.0
29	37.68321595195613	40.0	36.0	41.0	33.0	41.0
30	37.604281100431294	40.0	36.0	41.0	33.0	41.0
31	37.51718468191136	40.0	36.0	41.0	33.0	41.0
32	37.38870096601212	40.0	35.0	41.0	33.0	41.0
33	37.28898031517926	40.0	35.0	41.0	33.0	41.0
34	37.18735226726738	40.0	35.0	41.0	32.0	41.0
35	37.09574409008709	40.0	35.0	41.0	32.0	41.0
36	37.01824340801155	39.0	35.0	41.0	32.0	41.0
37	36.960928137809034	39.0	35.0	41.0	32.0	41.0
38	36.88486806365395	39.0	35.0	41.0	31.0	41.0
39	36.926397812207505	39.0	35.0	41.0	32.0	41.0
40	36.836646329145914	39.0	35.0	41.0	31.0	41.0
41	36.73923467503024	39.0	35.0	41.0	31.0	41.0
42	36.633375746762056	39.0	35.0	41.0	31.0	41.0
43	36.60432697453826	39.0	35.0	41.0	31.0	41.0
44	36.58700748713714	39.0	35.0	41.0	31.0	41.0
45	36.54253938131121	39.0	35.0	41.0	31.0	41.0
46	36.465251168783716	39.0	35.0	41.0	31.0	41.0
47	36.3345663005591	39.0	35.0	41.0	31.0	41.0
48	36.30300250054123	38.0	35.0	40.0	31.0	41.0
49	36.28980041544235	38.0	35.0	40.0	31.0	41.0
50	36.1662461540195	38.0	35.0	40.0	31.0	41.0
51	35.272618550362125	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	18.0
10	17.0
11	23.0
12	18.0
13	15.0
14	31.0
15	40.0
16	92.0
17	146.0
18	301.0
19	545.0
20	1028.0
21	1744.0
22	2754.0
23	3925.0
24	5346.0
25	7106.0
26	9266.0
27	11036.0
28	12030.0
29	14455.0
30	17005.0
31	21449.0
32	28448.0
33	39834.0
34	75016.0
35	101767.0
36	93443.0
37	141900.0
38	254917.0
39	398490.0
40	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13724888956493	20.483995972736295	23.615668341473974	11.763086796224803
2	31.392375723422596	26.170534175807287	23.48351872106209	18.95357137970803
3	31.311975314901602	25.875813158786382	26.431614181054634	16.38059734525738
4	28.506733433612517	25.560649995855233	27.829486749223964	18.10312982130828
5	27.440200687145833	28.72483664391472	23.774215693612476	20.060746975326975
6	26.15387463169933	35.4322749291567	23.156444386498205	15.25740605264577
7	78.15499170644435	6.4656736934531205	10.563438658673304	4.815895941429228
8	78.20167062230237	7.690029464053613	8.729359669899583	5.378940243744422
9	72.75528739323204	6.943408252993286	10.640780793396704	9.660523560377971
10	42.64891580169831	23.30283912433573	18.808947221437535	15.239297852528427
11	32.181329882312795	24.600271542520872	23.295837286957024	19.92256128820931
12	28.462227501768567	21.129372224918335	28.71702999764191	21.69137027567119
13	25.564754521215168	22.75822494569552	30.9291277239763	20.74789280911301
14	20.51989044941333	27.4468806009669	28.63405420065978	23.399174748959986
15	18.865686248471867	24.69725101426041	34.734183694410845	21.70287904285688
16	21.018147635753152	23.949744513416565	31.701824743205602	23.33028310762468
17	22.37891851390428	24.470133944344248	29.18623358290457	23.964713958846904
18	22.596377877091196	24.47488231681946	29.113237416209337	23.81550238988001
19	23.950951726757722	26.53736606973991	26.49607937347237	23.015602830029998
20	26.27540077470904	25.362103641679766	28.44331449275712	19.91918109085407
21	25.60032707433456	25.76829069053408	28.26030095023786	20.371081284893496
22	22.278397883030685	24.196418439459457	27.5760524284706	25.949131249039258
23	23.039344692406065	26.269364708003263	27.99753084631289	22.693759753277785
24	23.508548277668726	23.719408207923987	28.482508685899987	24.289534828507296
25	22.433404076035124	26.468957313741065	26.844078739282963	24.253559870940848
26	22.279202691924787	26.910234030378316	27.721239952966968	23.089323324729925
27	23.37985933550149	26.980010961497136	27.285516417699036	22.35461328530234
28	20.488422421653866	26.556761964087816	29.805051141581174	23.14976447267714
29	23.66435927956727	25.46471677567803	27.92445419872824	22.94646974602646
30	23.758843843735086	25.994522470666727	26.526340187890685	23.7202934977075
31	23.039666615963704	26.429038792593506	25.52684802230286	25.004446569139926
32	25.298362777266725	27.57017732354364	25.103920948451186	22.02753895073845
33	24.496290233402625	24.5803927628365	25.479686221108366	25.443630782652505
34	22.365800128930385	25.75018249041674	27.247127033450276	24.636890347202606
35	23.729709761768518	25.572319724819742	25.60942141483794	25.088549098573797
36	22.943733395786502	26.62694129965369	27.339438613604006	23.0898866909558
37	24.32760228919842	25.743502576595674	28.273499816101165	21.655395318104738
38	22.92860298857735	26.06631142402081	26.563441877908883	24.441643709492965
39	23.992962751029953	23.53727995518824	27.11296539080313	25.356791902978674
40	23.13624368325619	24.674796846114905	27.94071133838914	24.24824813223976
41	20.043282622324917	26.80898907150003	27.346199008314482	25.801529297860576
42	22.17031204855251	25.92192870841854	28.77924172515615	23.128517517872794
43	23.732928997344935	24.519871133999878	28.581017294538324	23.166182574116863
44	23.05415317605758	23.854455140354645	27.72204476186107	25.3693469217267
45	22.70293457467057	23.444485489698046	26.532295773707055	27.32028416192433
46	23.671683040503616	25.358723444324525	26.654465763832047	24.315127751339805
47	20.74901954156476	25.109393648931093	30.460326543160694	23.681260266343454
48	21.46618474710088	24.043665711358507	28.614899748980104	25.875249792560506
49	22.14632874350821	22.502939564485715	30.615493697943958	24.73523799406212
50	21.582640594077734	23.56931134917358	28.85030635050554	25.997741706243144
51	20.704191686163163	23.373018459901605	26.47781021157621	29.444979642359026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	341.0
1	850.0
2	1359.0
3	4320.5
4	7282.0
5	5001.5
6	2721.0
7	2671.5
8	2622.0
9	2586.0
10	2550.0
11	2626.5
12	2703.0
13	2563.0
14	2423.0
15	2439.0
16	2455.0
17	2436.0
18	2417.0
19	2465.5
20	2514.0
21	2882.5
22	3251.0
23	3558.5
24	3866.0
25	4999.5
26	7339.5
27	8546.0
28	9038.0
29	9530.0
30	12821.0
31	16112.0
32	17007.0
33	17902.0
34	22853.5
35	27805.0
36	30545.0
37	33285.0
38	35617.5
39	37950.0
40	42901.5
41	47853.0
42	55261.5
43	62670.0
44	69903.0
45	77136.0
46	98684.5
47	120233.0
48	121288.5
49	122344.0
50	122853.5
51	123363.0
52	107792.0
53	92221.0
54	82646.0
55	73071.0
56	69689.0
57	66307.0
58	63784.5
59	61262.0
60	59267.5
61	57273.0
62	52267.5
63	47262.0
64	39727.0
65	32192.0
66	26803.0
67	21414.0
68	18340.0
69	15266.0
70	13138.0
71	11010.0
72	9227.5
73	7445.0
74	6136.5
75	3682.5
76	2537.0
77	2018.0
78	1499.0
79	1247.0
80	995.0
81	645.5
82	296.0
83	246.5
84	197.0
85	118.5
86	40.0
87	30.0
88	20.0
89	18.5
90	17.0
91	13.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1242531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.034373349174146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.30142214749785	16.423835780725394
2	11.552521880585399	5.32210204243815
3	4.6487741680847146	3.2124479941097928
4	2.4125914668596513	2.222901303467076
5	1.474566189697008	1.6982854070775013
6	0.9434773397778012	1.30394455745565
7	0.6545895238550243	1.0554641638054334
8	0.49499691339494595	0.9121554967862403
9	0.36055831385604253	0.7474711333957914
>10	3.970363279177454	22.708444225677503
>50	1.8549698508739803	30.618880768651756
>100	0.3234016380891181	9.828755836381136
>500	0.003883644125523496	0.698986880584499
>1k	0.002824468454926179	1.7139207106827596
>5k	0.001059175670597317	1.5324036987613399
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC	6989	0.5624809360893209	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG	6271	0.50469565749265	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC	5583	0.4493248055782914	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	4757	0.38284759092529685	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3826	0.30791988288420974	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	3289	0.26470164527082224	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2594	0.20876742713059068	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT	2335	0.18792287677329578	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCCATT	1495	0.12031892966855556	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	1464	0.11782402209683299	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCT	1315	0.10583236957468264	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.609617788208101E-4	0.0	0.0	0.1987877968437005	0.0
2	1.609617788208101E-4	0.0	0.0	1.0962301946591273	0.0
3	1.609617788208101E-4	0.0	0.0	1.5293783414659272	0.0
4	1.609617788208101E-4	0.0	0.0	2.0694855902991556	0.0
5	1.609617788208101E-4	0.0	0.0	3.7909718147877194	0.0
6	1.609617788208101E-4	0.0	0.0	4.506929806982683	0.0
7	1.609617788208101E-4	0.0	0.0	5.294032905416445	0.0
8	1.609617788208101E-4	0.0	0.0	6.412395344663433	0.0
9	1.609617788208101E-4	0.0	0.0	6.785826671527713	0.0
10	1.609617788208101E-4	0.0	0.0	8.161888918666818	0.0
11	1.609617788208101E-4	0.0	0.0	9.508092755834664	0.0
12	1.609617788208101E-4	0.0	0.0	11.10491408262651	0.0
13	1.609617788208101E-4	0.0	0.0	11.543373968134397	0.0
14	1.609617788208101E-4	0.0	0.0	11.732101653801797	0.0
15	1.609617788208101E-4	0.0	0.0	12.149475546284156	0.0
16	1.609617788208101E-4	0.0	0.0	12.905915425852553	0.0
17	1.609617788208101E-4	0.0	0.0	13.695513431858037	0.0
18	1.609617788208101E-4	0.0	0.0	14.55416404097765	0.0
19	1.609617788208101E-4	0.0	0.0	15.214831662147665	8.048088941040505E-5
20	1.609617788208101E-4	0.0	0.0	15.767976815065378	8.048088941040505E-5
21	1.609617788208101E-4	0.0	0.0	16.429127321571855	8.048088941040505E-5
22	1.609617788208101E-4	0.0	0.0	17.12754047987535	8.048088941040505E-5
23	1.609617788208101E-4	0.0	0.0	17.838347695148048	8.048088941040505E-5
24	1.609617788208101E-4	0.0	0.0	18.396402182319797	8.048088941040505E-5
25	1.609617788208101E-4	0.0	0.0	18.880172808565742	8.048088941040505E-5
26	1.609617788208101E-4	0.0	0.0	19.334165505729835	8.048088941040505E-5
27	1.609617788208101E-4	0.0	0.0	19.792101766475042	8.048088941040505E-5
28	2.4144266823121515E-4	0.0	0.0	20.245531097413263	8.048088941040505E-5
29	2.4144266823121515E-4	0.0	0.0	20.716424781353545	8.048088941040505E-5
30	2.4144266823121515E-4	0.0	0.0	21.211221289448712	8.048088941040505E-5
31	2.4144266823121515E-4	0.0	0.0	21.711651459802614	8.048088941040505E-5
32	2.4144266823121515E-4	0.0	0.0	22.19228333136155	8.048088941040505E-5
33	2.4144266823121515E-4	0.0	0.0	22.66309653441242	8.048088941040505E-5
34	2.4144266823121515E-4	0.0	0.0	23.139140995274968	8.048088941040505E-5
35	2.4144266823121515E-4	0.0	0.0	23.601664666716566	8.048088941040505E-5
36	2.4144266823121515E-4	0.0	0.0	24.090183665437724	8.048088941040505E-5
37	4.0240444705202526E-4	0.0	0.0	24.576690641923623	8.048088941040505E-5
38	4.0240444705202526E-4	0.0	0.0	25.014426199426815	8.048088941040505E-5
39	4.0240444705202526E-4	0.0	0.0	25.476869389979	8.048088941040505E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	3.8909882E-5	45.000004	1
AAGCGTA	20	7.0335076E-4	45.0	37
TAACCCG	20	7.0335076E-4	45.0	1
AACGCGA	20	7.0335076E-4	45.0	12
GCGTGAC	20	7.0335076E-4	45.0	45
TAAGCGT	20	7.0335076E-4	45.0	17
TATCGCG	45	3.8562575E-10	45.0	1
CGTACTA	30	2.1656451E-6	44.999996	33
CGGTATT	30	2.1656451E-6	44.999996	43
CTAACGG	100	0.0	42.750004	2
GCTAACG	95	0.0	42.63158	1
CGTTTTT	2215	0.0	41.139957	1
CGCATCG	45	1.9284926E-8	40.0	21
ATGCGCG	45	1.9284926E-8	40.0	1
TGAACGG	130	0.0	39.80769	2
CCCTCGT	340	0.0	39.705883	14
GTATGCG	80	0.0	39.375	1
GCCCTCG	350	0.0	39.214287	13
ACCGGTA	35	6.2486997E-6	38.571426	41
GTTTACG	35	6.2486997E-6	38.571426	1
>>END_MODULE