FastQCFastQC Report
Sat 14 Jan 2017
SRR2934837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991021
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.20100240027603927No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC35080.17619100953731778No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC34010.17081688239350565No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT23760.11933575788502483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG1000.045.01
ATACGGA253.891839E-545.038
GACGATA302.1663072E-644.9999969
TATAGCG1450.041.8965531
GCCGATA950.040.2631579
GGACGTA1200.039.3749968
CGTTTTT27600.038.7228281
AATAGCG1050.038.571431
CGTCGAA356.250595E-638.57142630
TAAGGGA19950.037.7819564
TGCGACG900.037.51
CCGTAAA301.1402222E-437.49999632
CGTAATC301.1402222E-437.49999623
CGTCGCA301.1402222E-437.49999614
TACGTAA301.1402222E-437.49999645
ACGTATG1150.037.1739121
GCGATAT850.037.0588239
CGAATAT2250.036.00000414
CGAACTT504.8819857E-836.027
CATATCG250.002107478436.038
Produced by FastQC (version 0.11.5)