FastQCFastQC Report
Sat 14 Jan 2017
SRR2934836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868421
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC175950.941704251878993No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC155750.8335915727772274No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG154950.8293098825157713No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC108760.5820957910449519TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC53420.28590986720872863TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.2831267685387822No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT46200.24726761259908767TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCCCCT38860.20798310445022827No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT34330.18373803334473332No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG30610.16382817362896263No Hit
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC29080.15563944100392793TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT26870.14381127165665553No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC24810.13278591923340616No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTA24050.1287183134850229No Hit
ACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC20790.11127042566958945TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA302.1662345E-645.00000423
CGCTAAC302.1662345E-645.00000439
TAGCGAA207.034422E-445.01
TGCGCAC253.8917464E-545.013
CGAATCG253.8917464E-545.021
CGTCGGA207.034422E-445.023
AGTCGAG406.8193913E-945.01
GCTAACG1150.043.043481
CGTTTTT42650.041.8874551
TACGGCT21000.041.1428537
GCGTAAG1000.040.51
CGTCATA1400.040.1785738
ACGTTAG403.4597724E-739.3751
TCGAGTA403.4597724E-739.37526
TACGAAT1450.038.79310212
GTCGACG356.2503896E-638.5714261
TAACGCG700.038.5714261
ACGGCTG22400.038.5714268
GCGATCG356.2503896E-638.5714269
TATCGAG356.2503896E-638.5714261
Produced by FastQC (version 0.11.5)