##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934836.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868421
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10744527063226	33.0	31.0	34.0	30.0	34.0
2	32.2807311628375	34.0	31.0	34.0	30.0	34.0
3	32.295759360443924	34.0	31.0	34.0	30.0	34.0
4	35.882240137527894	37.0	35.0	37.0	35.0	37.0
5	35.751117654961064	37.0	35.0	37.0	35.0	37.0
6	35.66375351165503	37.0	35.0	37.0	33.0	37.0
7	35.98006819662164	37.0	35.0	37.0	35.0	37.0
8	35.716264696232805	37.0	35.0	37.0	35.0	37.0
9	37.48509195732653	39.0	37.0	39.0	35.0	39.0
10	37.194847413939364	39.0	37.0	39.0	34.0	39.0
11	37.09051172085948	39.0	37.0	39.0	34.0	39.0
12	37.03189484596887	39.0	37.0	39.0	33.0	39.0
13	36.98767943627266	39.0	37.0	39.0	33.0	39.0
14	38.12128797524755	40.0	37.0	41.0	33.0	41.0
15	38.12231076400875	40.0	37.0	41.0	33.0	41.0
16	38.26435530322128	40.0	37.0	41.0	34.0	41.0
17	38.19513963929971	40.0	37.0	41.0	34.0	41.0
18	38.19342696319512	40.0	37.0	41.0	34.0	41.0
19	38.15432817336136	40.0	37.0	41.0	34.0	41.0
20	38.0316652403286	40.0	37.0	41.0	34.0	41.0
21	38.03377076151467	40.0	37.0	41.0	34.0	41.0
22	37.981759464274916	40.0	37.0	41.0	33.0	41.0
23	37.748086753467234	40.0	36.0	41.0	33.0	41.0
24	37.487497732042186	39.0	36.0	41.0	32.0	41.0
25	37.432836603741876	39.0	36.0	41.0	32.0	41.0
26	37.56352610038102	39.0	36.0	41.0	33.0	41.0
27	37.582261171331304	39.0	36.0	41.0	33.0	41.0
28	37.48704387287447	39.0	36.0	41.0	33.0	41.0
29	37.463358632770664	39.0	36.0	41.0	33.0	41.0
30	37.31848068502762	39.0	36.0	41.0	32.0	41.0
31	37.15400222969021	39.0	35.0	41.0	32.0	41.0
32	37.03409831081967	39.0	35.0	41.0	31.0	41.0
33	36.92713365991926	39.0	35.0	41.0	31.0	41.0
34	36.7885701348893	39.0	35.0	41.0	31.0	41.0
35	36.745454584379004	39.0	35.0	41.0	31.0	41.0
36	36.67734841344643	39.0	35.0	41.0	30.0	41.0
37	36.57463869224334	39.0	35.0	41.0	30.0	41.0
38	36.55436274801022	39.0	35.0	41.0	30.0	41.0
39	36.6509908634082	39.0	35.0	41.0	31.0	41.0
40	36.63782038416395	39.0	35.0	41.0	31.0	41.0
41	36.571400128771835	39.0	35.0	41.0	30.0	41.0
42	36.45183392822067	39.0	35.0	41.0	30.0	41.0
43	36.38576851790897	39.0	35.0	41.0	30.0	41.0
44	36.36135860172841	39.0	35.0	41.0	30.0	41.0
45	36.34377744630359	39.0	35.0	41.0	30.0	41.0
46	36.25145885215377	39.0	35.0	40.0	30.0	41.0
47	36.05767864951208	39.0	35.0	40.0	30.0	41.0
48	36.043505719535375	39.0	35.0	40.0	29.0	41.0
49	36.073326086572564	39.0	35.0	40.0	29.0	41.0
50	35.98690980244816	39.0	35.0	40.0	29.0	41.0
51	35.09810690417203	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	4.0
9	28.0
10	33.0
11	34.0
12	21.0
13	24.0
14	33.0
15	56.0
16	97.0
17	204.0
18	386.0
19	800.0
20	1460.0
21	2746.0
22	4757.0
23	6933.0
24	9849.0
25	13263.0
26	16097.0
27	18125.0
28	19725.0
29	22691.0
30	28499.0
31	36822.0
32	48409.0
33	66267.0
34	110525.0
35	145380.0
36	152919.0
37	238029.0
38	417992.0
39	505895.0
40	314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.177130850060024	20.452510435281983	23.170580934382563	11.19977778027543
2	31.024324817586614	27.540741620865962	24.33926829124699	17.09566527030043
3	29.03489095872932	28.07857543883311	26.26340637361708	16.623127228820486
4	26.852299347952098	23.6486851732024	31.0852853826841	18.413730096161412
5	27.35229372823363	28.440699392695755	25.003840140953244	19.20316673811737
6	24.158688004470086	36.022716507682155	25.50752747908528	14.31106800876248
7	76.49154018286029	5.484898746053486	12.903355293052263	5.120205778033966
8	76.15981622985399	7.5433213392484895	10.189941132111018	6.106921298786515
9	71.49769778866755	5.80067340283587	11.752865119799019	10.948763688697568
10	40.907482842464304	21.022671014723127	21.37189637667314	16.69794976613943
11	29.5561867480616	24.552442945139237	24.33819786868163	21.55317243811753
12	25.016471127224538	21.457637224158795	30.576406495110042	22.949485153506625
13	24.165217582118807	23.605011932535547	33.210395301701276	19.01937518364437
14	20.454490717027905	28.28570220523105	29.498865619686356	21.76094145805469
15	18.49797235205556	26.18055566705791	35.43195029385775	19.88952168702878
16	20.52107100059355	25.175321835924557	30.244950147745076	24.058657015736816
17	20.19625127313384	26.686597934833745	29.301105050735355	23.816045741297064
18	21.165518906070954	25.74949649998582	30.513198042625294	22.57178655131793
19	20.882017489634293	28.792921937828787	27.08211907273575	23.24294149980117
20	23.26162037356677	26.725882442982602	30.04601211397217	19.966485069478455
21	21.74028230254316	28.91537827930643	29.499454352097302	19.8448850660531
22	20.133792116444848	24.61961196111583	28.945617716777967	26.30097820566136
23	20.507904803039573	28.269271218852708	28.255141640989905	22.96768233711781
24	22.027369634573795	24.93222887133039	28.736724753147175	24.30367674094864
25	19.400980828196644	30.190090991270168	26.38837820812333	24.020549972409857
26	19.05250476204239	28.327662769793317	29.165161384934123	23.45467108323017
27	21.909837236896823	29.263426176434542	26.756175401582404	22.07056118508623
28	18.006701915681745	29.055550114240848	29.801634642299568	23.136113327777842
29	19.842797742050642	27.853144446567452	28.610628974947293	23.693428836434617
30	20.10216112963834	30.46310226656626	26.68793596304045	22.746800640754948
31	19.785048444649252	30.2364938094787	24.12363166545441	25.854826080417638
32	20.17832169516399	30.232800851628195	26.09449369280264	23.494383760405178
33	19.825831544389622	27.15892189180062	24.33107955862196	28.684167005187806
34	18.63033010226282	28.00450219730992	26.357175390342967	27.00799231008429
35	18.505465310013108	27.406082462143168	25.886135940454537	28.20231628738919
36	18.10475262266909	31.046268480176575	23.335586572833424	27.51339232432091
37	17.578318805023066	29.59595294636487	28.35945431998463	24.466273928627437
38	17.82778078388115	28.130223327611926	25.510845789037912	28.531150099469016
39	20.353282263472742	24.194975329435923	27.643234581499566	27.80850782559177
40	19.69582872382616	23.528637282496824	27.909555715762135	28.865978277914884
41	17.36878358785306	26.640355680010018	24.93667112497665	31.05418960716027
42	18.927318843023066	26.13361763756669	27.547538804156023	27.391524715254217
43	20.266899162447864	26.27480637393821	26.479042999409664	26.979251464204268
44	19.211194907357605	25.99478383083898	27.435037392536266	27.35898386926715
45	19.783068162903326	23.890975320872545	26.564837368023586	29.76111914820054
46	21.625051313381725	26.326400741588753	25.825014811972245	26.22353313305727
47	17.114558228579106	25.556178184681077	30.831488192436286	26.49777539430353
48	18.35994136225187	25.298259867556617	27.47378668940244	28.868012080789075
49	18.80400616349313	22.753704866301547	31.273091021777212	27.16919794842811
50	18.885572362973868	22.408172462202042	29.412107870763606	29.294147304060488
51	18.019493465337845	22.42401471616943	26.133510595310156	33.42298122318257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	490.0
1	860.0
2	1230.0
3	5819.5
4	10409.0
5	7359.5
6	4310.0
7	4255.5
8	4201.0
9	4214.0
10	4227.0
11	4294.5
12	4362.0
13	4387.5
14	4413.0
15	4453.0
16	4493.0
17	4507.5
18	4522.0
19	4415.5
20	4309.0
21	4941.0
22	5573.0
23	5461.5
24	5350.0
25	6757.0
26	9846.0
27	11528.0
28	13352.0
29	15176.0
30	17900.5
31	20625.0
32	24807.0
33	28989.0
34	34866.0
35	40743.0
36	45403.0
37	50063.0
38	56434.5
39	62806.0
40	71320.5
41	79835.0
42	90794.0
43	101753.0
44	119212.5
45	136672.0
46	188864.0
47	241056.0
48	236941.0
49	232826.0
50	223529.0
51	214232.0
52	177435.0
53	140638.0
54	120675.5
55	100713.0
56	89409.5
57	78106.0
58	71170.0
59	64234.0
60	60526.0
61	56818.0
62	49558.5
63	42299.0
64	35368.0
65	28437.0
66	23044.5
67	17652.0
68	15283.5
69	12915.0
70	10791.5
71	8668.0
72	7625.5
73	6583.0
74	5346.0
75	2995.5
76	1882.0
77	1616.0
78	1350.0
79	1080.5
80	811.0
81	639.0
82	467.0
83	355.5
84	244.0
85	171.0
86	98.0
87	56.0
88	14.0
89	12.5
90	11.0
91	9.5
92	8.0
93	5.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1868421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.07210670964763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.51841047910563	19.51528237254539
2	12.883398442343127	7.233282717127287
3	5.281808377946476	4.44814465216873
4	2.740671129491337	3.077456496125253
5	1.6160758408219604	2.26833267272188
6	1.0709347246336087	1.8038036321389055
7	0.7399680045394363	1.4540722549609255
8	0.571322093583794	1.2830571821330845
9	0.44305879343817034	1.119383435523964
>10	4.021848158698929	24.96664671935182
>50	0.9220969992505278	18.3953493219978
>100	0.17856721051695926	7.1587573416363455
>500	0.007181485054261291	1.3248350730545528
>1k	0.00349369543180279	2.1281270114482713
>5k	3.881883813114211E-4	0.5792986426780179
>10k+	7.763767626228422E-4	3.2441704743878725
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	17595	0.941704251878993	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	15575	0.8335915727772274	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	15495	0.8293098825157713	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	10876	0.5820957910449519	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	5342	0.28590986720872863	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5290	0.2831267685387822	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	4620	0.24726761259908767	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATCCCCT	3886	0.20798310445022827	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	3433	0.18373803334473332	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	3061	0.16382817362896263	No Hit
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	2908	0.15563944100392793	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	2687	0.14381127165665553	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTC	2481	0.13278591923340616	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTA	2405	0.1287183134850229	No Hit
ACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	2079	0.11127042566958945	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.605633848046024E-4	0.0	0.0	0.26396620461876635	0.0
2	1.605633848046024E-4	0.0	0.0	1.485211309442572	0.0
3	1.605633848046024E-4	0.0	0.0	2.153476116999327	0.0
4	1.605633848046024E-4	0.0	0.0	2.994025436451421	0.0
5	1.605633848046024E-4	0.0	0.0	5.795374811137319	0.0
6	1.605633848046024E-4	0.0	0.0	6.977067802170924	0.0
7	1.605633848046024E-4	0.0	0.0	8.266177697638808	0.0
8	1.605633848046024E-4	0.0	0.0	10.057850987545098	0.0
9	1.605633848046024E-4	0.0	0.0	10.642248187105583	0.0
10	1.605633848046024E-4	0.0	0.0	12.979141210680034	0.0
11	1.605633848046024E-4	0.0	0.0	15.032211691048216	0.0
12	1.605633848046024E-4	0.0	0.0	17.581851199488767	0.0
13	1.605633848046024E-4	0.0	0.0	18.26943713435034	0.0
14	1.605633848046024E-4	0.0	0.0	18.56283995951662	0.0
15	1.605633848046024E-4	0.0	0.0	19.170732934386844	0.0
16	1.605633848046024E-4	0.0	0.0	20.209524512944352	0.0
17	1.605633848046024E-4	0.0	0.0	21.506662577652467	0.0
18	1.605633848046024E-4	0.0	0.0	22.78571050100593	0.0
19	1.605633848046024E-4	0.0	0.0	23.859130249552965	0.0
20	2.1408451307280318E-4	0.0	0.0	24.656113370594742	0.0
21	2.1408451307280318E-4	0.0	0.0	25.61847677798526	0.0
22	2.1408451307280318E-4	0.0	0.0	26.68905990673408	0.0
23	2.1408451307280318E-4	0.0	0.0	27.84597261537951	0.0
24	2.1408451307280318E-4	0.0	0.0	28.663026159521863	0.0
25	2.1408451307280318E-4	0.0	0.0	29.429555758579035	0.0
26	2.1408451307280318E-4	0.0	0.0	30.090220565921705	0.0
27	2.1408451307280318E-4	0.0	0.0	30.790812134952454	0.0
28	2.67605641341004E-4	0.0	0.0	31.48139525299705	0.0
29	2.67605641341004E-4	0.0	0.0	32.19563470973619	0.0
30	2.67605641341004E-4	0.0	0.0	32.97479529506466	0.0
31	2.67605641341004E-4	0.0	0.0	33.70621503397789	0.0
32	2.67605641341004E-4	0.0	0.0	34.4299277304205	0.0
33	2.67605641341004E-4	0.0	0.0	35.10049394649279	0.0
34	2.67605641341004E-4	0.0	0.0	35.743978471661364	0.0
35	2.67605641341004E-4	0.0	0.0	36.383502433338094	0.0
36	2.67605641341004E-4	0.0	0.0	37.0346940009773	0.0
37	2.67605641341004E-4	0.0	0.0	37.71580387931842	0.0
38	2.67605641341004E-4	0.0	0.0	38.36694192582935	0.0
39	2.67605641341004E-4	0.0	0.0	39.03006870507236	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTTA	30	2.1662345E-6	45.000004	23
CGCTAAC	30	2.1662345E-6	45.000004	39
TAGCGAA	20	7.034422E-4	45.0	1
TGCGCAC	25	3.8917464E-5	45.0	13
CGAATCG	25	3.8917464E-5	45.0	21
CGTCGGA	20	7.034422E-4	45.0	23
AGTCGAG	40	6.8193913E-9	45.0	1
GCTAACG	115	0.0	43.04348	1
CGTTTTT	4265	0.0	41.887455	1
TACGGCT	2100	0.0	41.142853	7
GCGTAAG	100	0.0	40.5	1
CGTCATA	140	0.0	40.17857	38
ACGTTAG	40	3.4597724E-7	39.375	1
TCGAGTA	40	3.4597724E-7	39.375	26
TACGAAT	145	0.0	38.793102	12
GTCGACG	35	6.2503896E-6	38.571426	1
TAACGCG	70	0.0	38.571426	1
ACGGCTG	2240	0.0	38.571426	8
GCGATCG	35	6.2503896E-6	38.571426	9
TATCGAG	35	6.2503896E-6	38.571426	1
>>END_MODULE