##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934834.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615342
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15018739065783	33.0	31.0	34.0	30.0	34.0
2	32.34135557671379	34.0	31.0	34.0	30.0	34.0
3	32.3289767739587	34.0	31.0	34.0	30.0	34.0
4	35.89190091014782	37.0	35.0	37.0	35.0	37.0
5	35.78625764698745	37.0	35.0	37.0	35.0	37.0
6	35.718738199093444	37.0	35.0	37.0	33.0	37.0
7	36.068779862097315	37.0	35.0	37.0	35.0	37.0
8	35.93319433284097	37.0	35.0	37.0	35.0	37.0
9	37.66164440719055	39.0	38.0	39.0	35.0	39.0
10	37.238407717994086	39.0	37.0	39.0	34.0	39.0
11	37.153623814647304	39.0	37.0	39.0	34.0	39.0
12	37.12819514381474	39.0	37.0	39.0	34.0	39.0
13	37.1102819093418	39.0	37.0	39.0	33.0	39.0
14	38.28299394184018	40.0	38.0	41.0	33.0	41.0
15	38.351710659414536	40.0	38.0	41.0	34.0	41.0
16	38.424977497025395	40.0	38.0	41.0	34.0	41.0
17	38.41294908446633	40.0	38.0	41.0	34.0	41.0
18	38.374647597846156	40.0	38.0	41.0	34.0	41.0
19	38.29528050406663	40.0	37.0	41.0	34.0	41.0
20	38.221675657538775	40.0	37.0	41.0	34.0	41.0
21	38.17443055402509	40.0	37.0	41.0	34.0	41.0
22	38.124665860232696	40.0	37.0	41.0	34.0	41.0
23	37.94326588425238	40.0	36.0	41.0	34.0	41.0
24	37.687027267290766	39.0	36.0	41.0	33.0	41.0
25	37.63289941077493	39.0	36.0	41.0	33.0	41.0
26	37.8085519970384	40.0	36.0	41.0	33.0	41.0
27	37.906883495878894	40.0	36.0	41.0	34.0	41.0
28	37.87184757159784	40.0	36.0	41.0	34.0	41.0
29	37.85793658556516	40.0	36.0	41.0	33.0	41.0
30	37.737227163040394	40.0	36.0	41.0	33.0	41.0
31	37.63905290644334	40.0	36.0	41.0	33.0	41.0
32	37.484431160707764	40.0	36.0	41.0	33.0	41.0
33	37.333276173095236	40.0	36.0	41.0	32.0	41.0
34	37.25509644397285	40.0	36.0	41.0	32.0	41.0
35	37.142387184880974	40.0	36.0	41.0	32.0	41.0
36	37.08334457966177	40.0	36.0	41.0	32.0	41.0
37	37.012839386334285	40.0	36.0	41.0	31.0	41.0
38	36.90947861195957	40.0	35.0	41.0	31.0	41.0
39	36.946668878788515	40.0	35.0	41.0	31.0	41.0
40	36.90865649503325	39.0	35.0	41.0	31.0	41.0
41	36.839965778144816	39.0	35.0	41.0	31.0	41.0
42	36.72469668961743	39.0	35.0	41.0	31.0	41.0
43	36.67575906526296	39.0	35.0	41.0	31.0	41.0
44	36.649520658783096	39.0	35.0	41.0	31.0	41.0
45	36.60034469480766	39.0	35.0	41.0	31.0	41.0
46	36.55045247384145	39.0	35.0	41.0	31.0	41.0
47	36.4205425228837	39.0	35.0	41.0	30.0	41.0
48	36.361707923151876	39.0	35.0	41.0	30.0	41.0
49	36.33525222522537	39.0	35.0	41.0	30.0	41.0
50	36.23745064512654	39.0	35.0	41.0	30.0	41.0
51	35.32219616650839	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	19.0
10	21.0
11	18.0
12	23.0
13	17.0
14	21.0
15	40.0
16	72.0
17	140.0
18	313.0
19	599.0
20	1120.0
21	1853.0
22	2857.0
23	4430.0
24	6821.0
25	10678.0
26	14631.0
27	16469.0
28	17363.0
29	18770.0
30	22109.0
31	27783.0
32	36573.0
33	50381.0
34	86951.0
35	119310.0
36	118195.0
37	186747.0
38	351487.0
39	519168.0
40	353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.217170110106714	23.055612990933188	25.88807819025321	12.839138708706887
2	32.477766318216204	23.932393264088965	25.940946251629686	17.648894166065144
3	29.82278675351721	23.94322688322349	30.160733764119303	16.073252599139998
4	26.811040634119585	24.916023975108676	30.002996269520633	18.269939121251102
5	24.107093110932546	29.759951762536975	27.988314548869525	18.144640577660955
6	23.83792410523592	33.79612490729517	27.086524092111762	15.279426895357146
7	80.3406956545425	4.666132620831997	10.142372327346159	4.850799397279338
8	82.21974046362938	3.3920371042169397	9.30069298018624	5.087529451967447
9	77.35389781235182	5.346917247245475	11.362671186658924	5.936513753743789
10	37.494474854241396	22.802601554345767	22.241543895967542	17.4613796954453
11	26.260630875690723	27.119891639046095	26.509185051834223	20.11029243342896
12	24.558576450064447	22.706522829221303	32.07983201080638	20.65506870990787
13	22.089811321689155	25.555207504045583	32.9543836537402	19.400597520525064
14	19.186463300031818	28.381358251070054	31.229609581128948	21.202568867769177
15	18.582442603485823	27.122986958798815	34.72298745404998	19.57158298366538
16	19.856847652076155	25.820414500458728	32.875205374465594	21.447532472999526
17	20.636001540231106	26.417192148783354	29.084924430863556	23.86188188012198
18	21.551101871925574	26.167152219158545	31.719846323564916	20.561899585350965
19	21.2931379237338	29.52408839737963	28.580882562330455	20.60189111655612
20	22.154379691730917	27.467124608906353	29.95786650752596	20.420629191836774
21	20.76006195592017	27.895021611522512	30.939330494718764	20.40558593783855
22	20.019723377464338	25.905411980868447	28.920191513623738	25.154673128043477
23	19.021482757211785	27.590689773434974	30.20815406273099	23.17967340662225
24	20.109549556688304	25.564864901674074	31.81140588185041	22.514179659787214
25	19.165972283268808	28.764992181222304	28.960554483199225	23.108481052309664
26	19.986108204949787	28.905210166020574	29.40597099561579	21.70271063341385
27	20.42069109823183	27.85954924715633	28.923101114191297	22.79665854042054
28	18.37165132832552	26.76083454773045	30.239850136998854	24.62766398694518
29	19.947973865596264	25.49497258165763	29.733022480688298	24.824031072057807
30	20.355379851449413	26.662031941223592	29.25225741669566	23.730330790631335
31	19.200701770894337	28.0894076919934	26.797545039997722	25.912345497114543
32	19.866938394470026	29.661025343240006	26.313313217882033	24.158723044407935
33	19.161019771664453	28.596482973884168	27.466753170536023	24.775744083915356
34	18.19484666405009	26.83660797527706	28.383648787687065	26.584896572985784
35	19.14671939440688	27.976057082648754	27.44279539564996	25.434428127294407
36	19.515371976955965	28.954240030903673	26.755572504150827	24.77481548798954
37	17.89385777129549	29.804091022210777	28.062602222934835	24.2394489835589
38	17.038559017223598	30.826227510954336	28.23024474074221	23.904968731079858
39	18.93908534539435	28.381729689440377	29.729493816170198	22.949691148995075
40	20.350179714264844	26.113293655461195	30.27086524092112	23.265661389352843
41	18.322435744257255	28.274260187625906	27.632786122071984	25.770517946044862
42	19.95732173124948	28.521576235868316	26.69837099512054	24.822731037761663
43	19.66301872916076	27.709054800779032	28.26088840629415	24.367038063766064
44	19.129633229371862	27.011555447700857	27.65507242429158	26.20373889863571
45	19.82917549348683	25.828276612630642	27.69407345317586	26.648474440706675
46	20.603191150852265	26.97614498972973	27.526121403393212	24.894542456024794
47	18.012532330614818	27.23280890362536	29.795362220508103	24.959296545251718
48	17.727453381389203	26.227696673521766	29.551265304808517	26.49358464028051
49	18.754604288132175	25.59383709455954	30.51855272753386	25.133005889774424
50	18.112449252232654	24.71730444698398	30.644594147864662	26.5256521529187
51	17.853928146485387	24.6510026978807	28.16542874512023	29.329640410513687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	671.5
2	1019.0
3	6744.0
4	12469.0
5	8778.0
6	5087.0
7	4995.5
8	4904.0
9	5012.5
10	5121.0
11	5056.5
12	4992.0
13	4903.5
14	4815.0
15	4850.0
16	4885.0
17	4786.5
18	4688.0
19	4746.0
20	4804.0
21	5057.5
22	5311.0
23	5795.0
24	6279.0
25	7307.0
26	10414.0
27	12493.0
28	15219.0
29	17945.0
30	21182.0
31	24419.0
32	29555.0
33	34691.0
34	42139.5
35	49588.0
36	53455.5
37	57323.0
38	61990.0
39	66657.0
40	74045.5
41	81434.0
42	88807.0
43	96180.0
44	108209.5
45	120239.0
46	139357.0
47	158475.0
48	177275.5
49	196076.0
50	192157.5
51	188239.0
52	158821.0
53	129403.0
54	110003.0
55	90603.0
56	78401.5
57	66200.0
58	57930.0
59	49660.0
60	42695.5
61	35731.0
62	31985.5
63	28240.0
64	22177.0
65	16114.0
66	12958.5
67	9803.0
68	7254.0
69	4705.0
70	4195.5
71	3686.0
72	2949.5
73	2213.0
74	1598.0
75	885.0
76	787.0
77	456.0
78	125.0
79	130.5
80	136.0
81	103.5
82	71.0
83	68.5
84	66.0
85	39.0
86	12.0
87	7.5
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1615342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.259641929547453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.90806741710504	21.456527299578095
2	12.501113605856892	7.565584428676468
3	5.048576480124204	4.5830434962748035
4	2.542025015194967	3.0768306694300844
5	1.48690453300236	2.2496599376036195
6	0.9632464531443541	1.7488495657232859
7	0.7046572372407088	1.4925872977377614
8	0.5249790014834049	1.2708541284335357
9	0.44449570995182786	1.2105252920126064
>10	3.7813544771016083	24.564594293333084
>50	0.8819617098778468	18.965562407065427
>100	0.20658516146103556	8.64616043936673
>500	0.0027045372387875803	0.48819710537012134
>1k	0.0027045372387875803	1.519108233357369
>5k	6.241239781817493E-4	1.161915406036922
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7063	0.437244868269382	No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	5831	0.3609761895623341	No Hit
CCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	5563	0.3443852756877491	No Hit
CGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTG	4158	0.2574067906362863	No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT	3954	0.24477788604518425	No Hit
TCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	2358	0.14597527953832687	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT	1790	0.11081244714741523	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	1703	0.1054265907776805	No Hit
ACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1678	0.10387893090131996	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTT	1647	0.10195983265463289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8571918516326573E-4	0.0	0.0	0.2596973272532999	0.0
2	1.8571918516326573E-4	0.0	0.0	1.2831957566880574	0.0
3	1.8571918516326573E-4	0.0	0.0	1.986266685321127	0.0
4	1.8571918516326573E-4	0.0	0.0	2.644641196724904	0.0
5	1.8571918516326573E-4	0.0	0.0	4.392073009926071	0.0
6	1.8571918516326573E-4	0.0	0.0	5.551889321270666	0.0
7	1.8571918516326573E-4	0.0	0.0	6.637975116105444	0.0
8	1.8571918516326573E-4	0.0	0.0	8.318548022647835	0.0
9	1.8571918516326573E-4	0.0	0.0	8.890501206555639	0.0
10	1.8571918516326573E-4	0.0	0.0	10.274356761602187	0.0
11	1.8571918516326573E-4	0.0	0.0	12.341968450024824	0.0
12	1.8571918516326573E-4	0.0	0.0	13.794168665211453	0.0
13	1.8571918516326573E-4	0.0	0.0	14.418123220964972	0.0
14	1.8571918516326573E-4	0.0	0.0	14.649529325678401	0.0
15	1.8571918516326573E-4	0.0	0.0	15.063311670222157	0.0
16	1.8571918516326573E-4	0.0	0.0	16.068361993930697	0.0
17	1.8571918516326573E-4	0.0	0.0	17.350876780273154	0.0
18	1.8571918516326573E-4	0.0	0.0	18.648744352589112	0.0
19	1.8571918516326573E-4	0.0	0.0	19.449132134247733	0.0
20	2.4762558021768766E-4	0.0	0.0	20.192689845246395	0.0
21	2.4762558021768766E-4	0.0	0.0	21.226898081025567	0.0
22	2.4762558021768766E-4	0.0	0.0	22.2759638516178	0.0
23	2.4762558021768766E-4	0.0	0.0	23.465433326193462	0.0
24	2.4762558021768766E-4	0.0	0.0	24.285507341479388	0.0
25	2.4762558021768766E-4	0.0	0.0	25.054632393635526	0.0
26	2.4762558021768766E-4	0.0	0.0	25.741545753159393	0.0
27	2.4762558021768766E-4	0.0	0.0	26.42474472897999	0.0
28	2.4762558021768766E-4	0.0	0.0	27.140073123833837	0.0
29	2.4762558021768766E-4	0.0	0.0	27.87118764942656	0.0
30	2.4762558021768766E-4	0.0	0.0	28.686185340318026	0.0
31	2.4762558021768766E-4	0.0	0.0	29.459396214547755	0.0
32	2.4762558021768766E-4	0.0	0.0	30.210382692952948	0.0
33	2.4762558021768766E-4	0.0	0.0	30.906520105339922	0.0
34	2.4762558021768766E-4	0.0	0.0	31.56365648884261	0.0
35	2.4762558021768766E-4	0.0	0.0	32.28356595693048	0.0
36	2.4762558021768766E-4	0.0	0.0	32.98768929427948	0.0
37	2.4762558021768766E-4	0.0	0.0	33.67918372703737	0.0
38	2.4762558021768766E-4	0.0	0.0	34.33551532740435	0.0
39	2.4762558021768766E-4	0.0	0.0	35.00540442828825	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	80	0.0	45.000004	2
ATTAGCG	70	0.0	45.000004	1
TCGATAT	20	7.034136E-4	45.000004	31
CGTAGCA	40	6.8175723E-9	45.000004	25
TAGTCCG	20	7.034136E-4	45.000004	1
GGTACGC	25	3.8915103E-5	45.0	8
GCGAACG	45	3.8562575E-10	45.0	1
GACGTAG	45	3.8562575E-10	45.0	1
GCGATCC	25	3.8915103E-5	45.0	9
TCGCGTA	45	3.8562575E-10	45.0	30
TCGCGCG	25	3.8915103E-5	45.0	1
ACGACCG	25	3.8915103E-5	45.0	16
CGTTTTT	5790	0.0	43.367874	1
TTGCTCG	205	0.0	42.80488	1
CGAAAGG	325	0.0	42.230766	2
ACGCGAG	65	0.0	41.53846	1
TGTTACG	115	0.0	41.086956	1
GCGTATG	55	6.184564E-11	40.909092	1
CGCGTAA	50	1.0822987E-9	40.5	31
ATGTCGA	95	0.0	40.263157	12
>>END_MODULE