##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934833.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730504 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1665644541303 33.0 31.0 34.0 30.0 34.0 2 32.34690843581966 34.0 31.0 34.0 30.0 34.0 3 32.334666203059804 34.0 31.0 34.0 30.0 34.0 4 35.897123082146024 37.0 35.0 37.0 35.0 37.0 5 35.799170161970366 37.0 35.0 37.0 35.0 37.0 6 35.719841369794004 37.0 35.0 37.0 33.0 37.0 7 36.037397468049456 37.0 35.0 37.0 35.0 37.0 8 35.89119292981284 37.0 35.0 37.0 35.0 37.0 9 37.617577727158235 39.0 38.0 39.0 35.0 39.0 10 37.31065675205064 39.0 37.0 39.0 34.0 39.0 11 37.197571813432916 39.0 37.0 39.0 34.0 39.0 12 36.96513913681513 39.0 35.0 39.0 33.0 39.0 13 36.83117683133836 39.0 35.0 39.0 33.0 39.0 14 37.91166099022045 40.0 37.0 41.0 33.0 41.0 15 38.03328934543822 40.0 37.0 41.0 33.0 41.0 16 38.15332291130507 40.0 37.0 41.0 34.0 41.0 17 38.16141321608095 40.0 36.0 41.0 34.0 41.0 18 38.11490696833967 40.0 36.0 41.0 34.0 41.0 19 38.0733849506642 40.0 36.0 41.0 34.0 41.0 20 37.987757767240154 40.0 36.0 41.0 34.0 41.0 21 37.919744450406846 40.0 36.0 41.0 34.0 41.0 22 37.830670331716185 40.0 35.0 41.0 33.0 41.0 23 37.65419354308806 39.0 35.0 41.0 33.0 41.0 24 37.39848789329011 39.0 35.0 41.0 33.0 41.0 25 37.33255396274353 39.0 35.0 41.0 33.0 41.0 26 37.50516492722832 39.0 35.0 41.0 33.0 41.0 27 37.57490171169494 39.0 35.0 41.0 33.0 41.0 28 37.559192009900016 39.0 35.0 41.0 33.0 41.0 29 37.57246640675479 39.0 35.0 41.0 33.0 41.0 30 37.426691708738076 39.0 35.0 41.0 33.0 41.0 31 37.32218577858574 39.0 35.0 41.0 33.0 41.0 32 37.18755544117486 39.0 35.0 41.0 33.0 41.0 33 37.005791891625506 39.0 35.0 41.0 32.0 41.0 34 36.933998992476425 39.0 35.0 41.0 32.0 41.0 35 36.88453588207594 39.0 35.0 41.0 32.0 41.0 36 36.77150433125623 39.0 35.0 41.0 31.0 41.0 37 36.67726665425514 39.0 35.0 41.0 31.0 41.0 38 36.581787368720775 39.0 35.0 41.0 31.0 41.0 39 36.56747806993528 39.0 35.0 41.0 31.0 41.0 40 36.498516092998806 39.0 35.0 41.0 31.0 41.0 41 36.449992060276195 39.0 35.0 41.0 31.0 41.0 42 36.36326152902654 39.0 35.0 41.0 31.0 41.0 43 36.28845564158444 39.0 35.0 41.0 30.0 41.0 44 36.1743987712593 39.0 35.0 40.0 30.0 41.0 45 36.11928887453046 38.0 35.0 40.0 30.0 41.0 46 36.03692656029262 38.0 35.0 40.0 30.0 41.0 47 35.92889019088191 38.0 35.0 40.0 30.0 41.0 48 35.88410741077393 38.0 35.0 40.0 30.0 41.0 49 35.866137625529774 38.0 35.0 40.0 30.0 41.0 50 35.74043947740191 38.0 35.0 40.0 29.0 41.0 51 34.823278175068175 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 7.0 10 20.0 11 11.0 12 16.0 13 19.0 14 12.0 15 24.0 16 46.0 17 103.0 18 193.0 19 362.0 20 639.0 21 1052.0 22 1570.0 23 2351.0 24 3519.0 25 4858.0 26 6453.0 27 7487.0 28 8198.0 29 9071.0 30 10852.0 31 13797.0 32 17853.0 33 25565.0 34 49604.0 35 70669.0 36 54440.0 37 83878.0 38 150538.0 39 207145.0 40 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.46890913670562 21.351149343466975 24.61985149978645 13.560090020040958 2 32.23185636218282 23.797405626800128 26.860085639503684 17.110652371513364 3 30.09346971406043 24.43915433727947 29.32536988161598 16.14200606704412 4 28.19847666816335 26.02833112481246 27.356455269238772 18.41673693778542 5 24.362358043213998 29.399565231675666 26.700743596202077 19.53733312890826 6 24.428066102307447 33.131372312814165 26.746465453987934 15.694096130890452 7 78.98943742950073 5.888674120880926 9.723423827932494 5.39846462168585 8 79.60928345361559 4.182317961297953 9.802957957793524 6.405440627292938 9 74.97275853383417 5.681009275787676 12.429226944684766 6.917005245693384 10 41.5587046751284 23.47160316712845 19.256020500914435 15.713671656828708 11 31.24034912882065 23.842853701006426 25.073237107531238 19.843560062641682 12 27.70292838916693 22.45436027728801 30.462393087512186 19.380318246032875 13 22.899806161225676 25.926346741427835 32.0040684239922 19.169778673354287 14 18.543224951540306 30.229813936679335 29.552884036227045 21.674077075553317 15 16.29272392758972 25.24777413949821 37.51642701477336 20.94307491813871 16 18.804140702857204 24.683369290243448 33.81473612738603 22.69775387951332 17 19.397019044385793 24.664615115043862 28.83338078915379 27.10498505141656 18 21.099268450275428 23.813285074414377 32.107832400643936 22.97961407466626 19 21.759634444164575 26.266796622605764 28.316340499162223 23.657228434067438 20 22.96428219421112 26.94646435885361 28.63201296639033 21.45724048054494 21 22.62082069365808 27.618876830243227 29.062811428821746 20.697491047276948 22 20.494891198405483 25.14428394642603 28.061584878385332 26.299239976783152 23 19.28476777676782 27.536194189217305 28.40011827450637 24.778919759508504 24 21.578252822708706 24.90280682925761 30.243229332077583 23.27571101595611 25 19.060675916901207 26.984383384622124 28.57465530647334 25.380285392003326 26 19.140346938552014 27.291568560883995 28.23516366782386 25.332920832740136 27 20.272852715385543 27.281164784860863 29.208601184935333 23.23738131481826 28 17.03741526398213 25.88815393207977 31.336720948824375 25.737709855113728 29 19.125973300625322 23.524854073352095 30.622282697972906 26.726889928049673 30 20.639038253041736 25.207117277934138 31.45020424255035 22.703640226473777 31 22.535400216836596 25.493905577519083 27.18342404695936 24.78727015868496 32 23.21903781498801 25.09294952525927 26.994513377065697 24.69349928268702 33 22.789334486874814 24.220538149003975 28.59956961221294 24.390557751908272 34 19.71597691456857 23.308154370133497 30.141381840482733 26.834486874815195 35 20.414125042436453 22.456961221293792 31.791749258046497 25.337164478223258 36 23.709247314183084 22.95045612344354 30.84172023698707 22.498576325386306 37 21.174011367494224 24.062017456441033 32.07237742709143 22.69159374897331 38 21.334037869744723 24.74483370385378 29.7368666016887 24.184261824712802 39 22.87543942264519 23.886248398366057 30.755752193006476 22.48255998598228 40 21.933623908972436 22.060522598096657 33.38886576938662 22.616987723544295 41 19.072722394401673 22.962502600944006 31.00913889588558 26.955636108768736 42 23.144842464928324 21.599060374754963 29.210517669992225 26.04557949032449 43 24.178101694172792 21.80097576467754 28.594915291360483 25.426007249789183 44 21.493927480205446 23.170441229616813 28.376846670244106 26.958784619933635 45 21.530340696286398 23.590699024235324 28.54481289630173 26.33414738317655 46 22.718972106928913 23.229030915641804 31.020637806226937 23.031359171202347 47 19.823573861334093 22.91637006778881 32.842393744592776 24.41766232628432 48 20.97907745885033 21.171957990647552 32.26238323130332 25.5865813191988 49 22.399466669587024 19.401810257028025 33.068128305936725 25.130594767448226 50 20.327746323086526 20.765526266796623 32.38476449136486 26.521962918751985 51 19.75211634706997 20.792083274013557 29.694019471488176 29.7617809074283 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 380.0 1 552.0 2 724.0 3 2904.0 4 5084.0 5 3415.5 6 1747.0 7 1757.0 8 1767.0 9 1907.5 10 2048.0 11 2174.5 12 2301.0 13 2332.5 14 2364.0 15 2425.0 16 2486.0 17 2310.0 18 2134.0 19 2150.5 20 2167.0 21 2208.5 22 2250.0 23 2328.0 24 2406.0 25 2399.5 26 3163.0 27 3933.0 28 4510.5 29 5088.0 30 6616.0 31 8144.0 32 9738.0 33 11332.0 34 13732.0 35 16132.0 36 16817.0 37 17502.0 38 20183.5 39 22865.0 40 25698.0 41 28531.0 42 35043.0 43 41555.0 44 49832.5 45 58110.0 46 65450.0 47 72790.0 48 80159.0 49 87528.0 50 86507.5 51 85487.0 52 72241.5 53 58996.0 54 50508.0 55 42020.0 56 38506.5 57 34993.0 58 31160.5 59 27328.0 60 25262.0 61 23196.0 62 20713.0 63 18230.0 64 14732.0 65 11234.0 66 9663.5 67 8093.0 68 7082.0 69 6071.0 70 5198.5 71 4326.0 72 3578.5 73 2831.0 74 2162.5 75 1295.5 76 1097.0 77 858.0 78 619.0 79 591.0 80 563.0 81 338.5 82 114.0 83 71.0 84 28.0 85 22.5 86 17.0 87 9.5 88 2.0 89 2.0 90 2.0 91 1.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 730504.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.244493366066934 #Duplication Level Percentage of deduplicated Percentage of total 1 69.63376317439418 18.27502835684664 2 12.56342760938423 6.594415850990931 3 5.301673417952049 4.174191985394879 4 2.8596998445274737 3.0020549439457573 5 1.650185815776921 2.1654145347467577 6 1.079788064921643 1.7003094423956584 7 0.7793571905824029 1.4317684232625505 8 0.5589650446543544 1.1735803525035609 9 0.4213026178567475 0.9951186383503239 >10 3.456692414185632 20.24547053379406 >50 1.3324651167080233 25.25880498861463 >100 0.3563446726536314 12.27603221569391 >500 0.0031675082013656124 0.5765041087253663 >1k 0.0031675082013656124 2.1313056247349937 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC 3796 0.5196412339973497 TruSeq Adapter, Index 20 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC 3554 0.4865134208710698 TruSeq Adapter, Index 20 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2981 0.40807442532826654 No Hit CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT 2513 0.3440090677121549 TruSeq Adapter, Index 27 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC 1377 0.18849999452432842 TruSeq Adapter, Index 20 (95% over 21bp) ACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC 1162 0.1590682597220549 TruSeq Adapter, Index 27 (95% over 22bp) CGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTG 987 0.13511219651090206 TruSeq Adapter, Index 27 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3689178977801628E-4 0.0 0.0 0.36399526901974527 0.0 2 1.3689178977801628E-4 0.0 0.0 1.800948386319582 0.0 3 1.3689178977801628E-4 0.0 0.0 2.5063517790457 0.0 4 1.3689178977801628E-4 0.0 0.0 3.2926582195306255 0.0 5 1.3689178977801628E-4 0.0 0.0 5.397506379157404 0.0 6 1.3689178977801628E-4 0.0 0.0 6.5306966149398225 0.0 7 1.3689178977801628E-4 0.0 0.0 7.697562231007633 0.0 8 1.3689178977801628E-4 0.0 0.0 9.506861016503674 0.0 9 1.3689178977801628E-4 0.0 0.0 10.139574868857665 0.0 10 1.3689178977801628E-4 0.0 0.0 11.757225148664483 0.0 11 1.3689178977801628E-4 0.0 0.0 13.937911359828282 0.0 12 1.3689178977801628E-4 0.0 0.0 15.574315814834689 0.0 13 1.3689178977801628E-4 0.0 0.0 16.188138600199313 0.0 14 1.3689178977801628E-4 0.0 0.0 16.417979915236604 0.0 15 1.3689178977801628E-4 0.0 0.0 16.88888767207298 0.0 16 1.3689178977801628E-4 0.0 0.0 17.899970431373408 0.0 17 1.3689178977801628E-4 0.0 0.0 19.127752893892435 0.0 18 1.3689178977801628E-4 0.0 0.0 20.51528807508241 0.0 19 1.3689178977801628E-4 0.0 0.0 21.287494661220197 0.0 20 1.3689178977801628E-4 0.0 0.0 22.052445982499755 0.0 21 1.3689178977801628E-4 0.0 0.0 22.94210572426708 0.0 22 1.3689178977801628E-4 0.0 0.0 23.85325747702956 0.0 23 1.3689178977801628E-4 0.0 0.0 24.74538127101289 0.0 24 1.3689178977801628E-4 0.0 0.0 25.45215358163679 0.0 25 1.3689178977801628E-4 0.0 0.0 26.10622255319615 0.0 26 1.3689178977801628E-4 0.0 0.0 26.667615783075796 0.0 27 1.3689178977801628E-4 0.0 0.0 27.23106238980211 0.0 28 1.3689178977801628E-4 0.0 0.0 27.779314007863064 0.0 29 1.3689178977801628E-4 0.0 0.0 28.350152771237394 0.0 30 1.3689178977801628E-4 0.0 0.0 29.049669817003057 0.0 31 2.7378357955603255E-4 0.0 0.0 29.642274374952088 0.0 32 2.7378357955603255E-4 0.0 0.0 30.172456276762343 0.0 33 2.7378357955603255E-4 0.0 0.0 30.697710074140595 0.0 34 4.106753693340488E-4 0.0 0.0 31.214613472342382 0.0 35 4.106753693340488E-4 0.0 0.0 31.831174093502568 0.0 36 4.106753693340488E-4 0.0 0.0 32.33151358514122 0.0 37 4.106753693340488E-4 0.0 0.0 32.85813630041724 0.0 38 4.106753693340488E-4 0.0 0.0 33.355601064470555 0.0 39 4.106753693340488E-4 0.0 0.0 33.865659873183446 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGCGG 30 2.16441E-6 45.000004 2 ACGCCCA 30 2.16441E-6 45.000004 22 CGTTGGC 35 1.2111195E-7 45.000004 21 TAAACGG 30 2.16441E-6 45.000004 2 ACGATGT 30 2.16441E-6 45.000004 29 GTCACGA 30 2.16441E-6 45.000004 42 GACGGTC 60 0.0 45.000004 29 GCTCGAT 35 1.2111195E-7 45.000004 19 CGATATA 30 2.16441E-6 45.000004 9 TAATCCG 30 2.16441E-6 45.000004 4 AACGTAA 25 3.8894046E-5 45.0 10 CTAGACA 40 6.8084773E-9 45.0 21 CCTTATC 20 7.031597E-4 45.0 42 CGGAAAC 20 7.031597E-4 45.0 16 AAATCCG 20 7.031597E-4 45.0 45 CTCCGTT 40 6.8084773E-9 45.0 22 ATTAGCG 50 2.1827873E-11 45.0 1 GATCGGA 20 7.031597E-4 45.0 9 CGAAATA 25 3.8894046E-5 45.0 23 GTACAAG 110 0.0 45.0 1 >>END_MODULE