##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934830.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1067476
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08154188009848	33.0	31.0	34.0	30.0	34.0
2	32.267191955603685	34.0	31.0	34.0	30.0	34.0
3	32.26146348957728	34.0	31.0	34.0	30.0	34.0
4	35.85261120624726	37.0	35.0	37.0	35.0	37.0
5	35.73331203699193	37.0	35.0	37.0	35.0	37.0
6	35.664889889796115	37.0	35.0	37.0	33.0	37.0
7	36.03106580382135	37.0	35.0	37.0	35.0	37.0
8	35.914896447320594	37.0	35.0	37.0	35.0	37.0
9	37.653682143673485	39.0	38.0	39.0	35.0	39.0
10	37.187575177334196	39.0	37.0	39.0	34.0	39.0
11	37.10897481535885	39.0	37.0	39.0	34.0	39.0
12	37.06749378908753	39.0	37.0	39.0	33.0	39.0
13	37.03929924419847	39.0	37.0	39.0	33.0	39.0
14	38.154092457347986	40.0	37.0	41.0	33.0	41.0
15	38.209657172620275	40.0	37.0	41.0	33.0	41.0
16	38.29993086495622	40.0	37.0	41.0	34.0	41.0
17	38.3005407147327	40.0	37.0	41.0	34.0	41.0
18	38.280509351029906	40.0	37.0	41.0	34.0	41.0
19	38.23179818562666	40.0	37.0	41.0	34.0	41.0
20	38.1795440834267	40.0	37.0	41.0	34.0	41.0
21	38.131314427678	40.0	37.0	41.0	34.0	41.0
22	38.083193439477796	40.0	37.0	41.0	34.0	41.0
23	37.87677381037138	40.0	36.0	41.0	33.0	41.0
24	37.60671902693831	39.0	36.0	41.0	33.0	41.0
25	37.56768489408661	39.0	36.0	41.0	33.0	41.0
26	37.77326703363823	39.0	36.0	41.0	33.0	41.0
27	37.891853306303844	40.0	37.0	41.0	34.0	41.0
28	37.8603696944943	40.0	36.0	41.0	34.0	41.0
29	37.892548403898545	40.0	37.0	41.0	34.0	41.0
30	37.75772007988938	40.0	36.0	41.0	33.0	41.0
31	37.666860894296455	40.0	36.0	41.0	33.0	41.0
32	37.61220111740217	40.0	36.0	41.0	33.0	41.0
33	37.535153951938966	40.0	36.0	41.0	33.0	41.0
34	37.49109581854768	40.0	36.0	41.0	33.0	41.0
35	37.45950260240043	40.0	36.0	41.0	33.0	41.0
36	37.36506019807471	40.0	36.0	41.0	33.0	41.0
37	37.261092521049655	40.0	36.0	41.0	32.0	41.0
38	37.19283337517658	40.0	36.0	41.0	32.0	41.0
39	37.2294149938734	40.0	36.0	41.0	32.0	41.0
40	37.1855067467559	40.0	36.0	41.0	32.0	41.0
41	37.13282921583249	39.0	36.0	41.0	32.0	41.0
42	37.02686805136602	39.0	35.0	41.0	32.0	41.0
43	36.950673364085	39.0	35.0	41.0	31.0	41.0
44	36.88570984265689	39.0	35.0	41.0	31.0	41.0
45	36.855676380546264	39.0	35.0	41.0	31.0	41.0
46	36.78303306116484	39.0	35.0	41.0	31.0	41.0
47	36.660470118297745	39.0	35.0	41.0	31.0	41.0
48	36.637074744537586	39.0	35.0	41.0	31.0	41.0
49	36.64474798496641	39.0	35.0	41.0	31.0	41.0
50	36.53796806672937	39.0	35.0	41.0	31.0	41.0
51	35.54927511250838	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	13.0
11	12.0
12	10.0
13	12.0
14	21.0
15	24.0
16	42.0
17	81.0
18	164.0
19	336.0
20	572.0
21	1005.0
22	1690.0
23	2531.0
24	3715.0
25	5618.0
26	7418.0
27	8574.0
28	9613.0
29	10987.0
30	13797.0
31	18148.0
32	24539.0
33	34460.0
34	59908.0
35	82049.0
36	82175.0
37	129616.0
38	245106.0
39	325072.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.65850286095425	22.41399338252101	27.02430780645185	10.903195950072881
2	32.553050373029464	23.635566513907573	26.382419838947197	17.428963274115763
3	29.983250208903993	23.413172755172013	29.14969516879068	17.453881867133312
4	27.36895255724719	26.331177469095323	27.948263005444616	18.35160696821287
5	24.548561279129462	28.3404966481682	27.819829204591016	19.29111286811132
6	24.167007033413398	33.785209222502424	26.74711187886191	15.300671865222263
7	79.17929770786417	5.3406352929714584	10.10842398330267	5.371643015861715
8	80.35880900366847	3.3360000599545097	9.594220385282668	6.710970551094357
9	75.3052059250044	5.0647508702771775	12.353064612225474	7.276978592492946
10	37.38463440864244	20.89114884081703	23.75828590057294	17.965930849967588
11	27.077330075804984	25.645354087586043	25.44469383855	21.832621998058972
12	23.154525253963556	22.863839561732537	32.351172298018874	21.63046288628503
13	22.09117582034631	25.978101615399318	34.11168026260075	17.81904230165362
14	19.838572483128427	28.699286916052447	30.687715695715877	20.774424905103253
15	18.227669755572958	27.45345094409617	34.99956907696286	19.31931022336802
16	19.602688959751788	26.365557633145855	31.789473486991742	22.242279920110615
17	20.10302807744624	26.635727641651897	28.02517339968299	25.236070881218875
18	20.65545267528263	26.004987465760355	32.74565423484931	20.59390562410771
19	20.4659402178597	29.152599215345358	28.636241002139627	21.74521956465532
20	20.51615211957927	28.668185514241067	29.914302522960707	20.90135984321896
21	19.841382850761985	28.66940334021561	31.33840948180568	20.150804327216722
22	19.253922336427237	26.347009206764366	28.144614024109206	26.254454432699188
23	18.27263563770989	27.968310294564	29.008333676822712	24.750720390903403
24	20.356991632598763	25.73790886165122	30.551225507646073	23.35387399810394
25	18.398540107693286	28.151546264271982	28.710153670902205	24.739759957132527
26	17.562268378867536	28.73488490607751	31.561646350831307	22.141200364223646
27	20.001386448032555	27.381224495913724	30.81521270735829	21.802176348695426
28	17.035137089733166	27.39846141739955	31.88736795956068	23.679033533306605
29	19.28783410587217	24.335254375742405	32.5375933510449	23.839318167340533
30	20.235583750829058	26.202181594715015	30.657644762036806	22.90458989241913
31	18.257459652488674	29.19934499698354	28.821631587033337	23.721563763494448
32	19.358561691316716	27.081264590491966	28.638770333009827	24.921403385181492
33	19.07677549659196	25.478792965837172	28.588652110211378	26.85577942735949
34	17.008344918293243	25.563853426212862	31.157890200810133	26.269911454683758
35	18.166591099003632	22.56219343573064	31.148522308698272	28.12269315656745
36	18.55835634712162	24.828848611116317	30.607339181396114	26.005455860365945
37	18.027665258984747	26.412771809389625	32.224331038824296	23.335231892801335
38	18.938880124705378	27.09231870318396	26.605750386893945	27.363050785216718
39	20.20476338578104	23.548538796188392	29.003181336161187	27.243516481869385
40	19.469852249605612	21.756273677347313	33.08702022340549	25.686853849641583
41	16.693490064413627	23.340665270226214	30.3740786678108	29.59176599754936
42	18.729976130610897	23.307502932150232	30.697270945669974	27.265249991568897
43	19.99445420786978	24.096841521495566	30.83413584942425	25.074568421210408
44	19.785081819169704	24.639804548298976	28.74565798200615	26.829455650525162
45	20.547534558154002	22.397505892404137	29.530031588532204	27.52492796090966
46	21.500998617299125	23.054007771603295	30.235433864555265	25.20955974654231
47	17.150924236235756	23.551630200585304	33.15418800984753	26.143257553331413
48	18.0949267243479	21.796180897743834	31.476492211534495	28.632400166373763
49	20.195582851511414	20.353619191438497	32.515672483503145	26.935125473546943
50	19.360341590817967	20.152677905639095	31.839216994105723	28.647763509437212
51	18.385237700894447	19.723534767994785	28.851234126106817	33.039993405003955
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	331.0
1	427.5
2	524.0
3	2573.5
4	4623.0
5	3327.5
6	2032.0
7	2051.5
8	2071.0
9	2225.0
10	2379.0
11	2392.0
12	2405.0
13	2496.5
14	2588.0
15	2532.0
16	2476.0
17	2365.5
18	2255.0
19	2346.5
20	2438.0
21	2648.5
22	2859.0
23	3073.5
24	3288.0
25	3708.0
26	4834.0
27	5540.0
28	6936.5
29	8333.0
30	10647.5
31	12962.0
32	15177.0
33	17392.0
34	20738.5
35	24085.0
36	27193.5
37	30302.0
38	34663.5
39	39025.0
40	44938.0
41	50851.0
42	58080.0
43	65309.0
44	76534.0
45	87759.0
46	105861.5
47	123964.0
48	141225.5
49	158487.0
50	153954.0
51	149421.0
52	121138.5
53	92856.0
54	75137.5
55	57419.0
56	46791.0
57	36163.0
58	30586.5
59	25010.0
60	21327.5
61	17645.0
62	15397.0
63	13149.0
64	10059.0
65	6969.0
66	5601.0
67	4233.0
68	3396.5
69	2560.0
70	2170.0
71	1780.0
72	1374.5
73	969.0
74	717.0
75	304.0
76	143.0
77	131.0
78	119.0
79	97.5
80	76.0
81	57.5
82	39.0
83	31.0
84	23.0
85	23.0
86	23.0
87	12.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1067476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.06306402846979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.42832024812701	23.64941318001056
2	13.126913929025697	8.942858193212286
3	5.409854603157611	5.528286711962093
4	2.7843123505264087	3.793688394849612
5	1.6546406885102882	2.818106585841864
6	1.1309435476080902	2.3114041484855488
7	0.7585776912244256	1.808763632672248
8	0.5971441551389561	1.6272447672579806
9	0.46130083314639153	1.4141987834266796
>10	4.117855365605518	29.55947853680197
>50	0.45736820602882666	10.28612467946501
>100	0.065574549471753	4.4246459536937985
>500	0.0041502879398892184	0.956896720984519
>1k	0.002490172763933531	1.7965493248701763
>5k	5.533717253185625E-4	1.0823403864656962
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	6366	0.5963600118410156	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	5118	0.4794487182850013	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT	4185	0.39204628488134624	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	2958	0.27710224866882255	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2556	0.2394433223791448	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	2335	0.21874028081193395	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGC	1980	0.1854842638148305	TruSeq Adapter, Index 22 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1467	0.13742697728098804	No Hit
GCCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1334	0.12496768077221408	TruSeq Adapter, Index 20 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1155	0.10819915389198445	TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1092	0.10229738186151258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.810367633558038E-4	0.0	0.0	0.4119998950796083	0.0
2	2.810367633558038E-4	0.0	0.0	2.046322352914726	0.0
3	2.810367633558038E-4	0.0	0.0	3.059085169127924	0.0
4	2.810367633558038E-4	0.0	0.0	4.100232698440059	0.0
5	2.810367633558038E-4	0.0	0.0	6.726708609842282	0.0
6	2.810367633558038E-4	0.0	0.0	8.257515859841345	0.0
7	2.810367633558038E-4	0.0	0.0	9.741764686044464	0.0
8	2.810367633558038E-4	0.0	0.0	12.182662654710738	0.0
9	2.810367633558038E-4	0.0	0.0	12.992516927781047	0.0
10	2.810367633558038E-4	0.0	0.0	15.05036178799336	0.0
11	2.810367633558038E-4	0.0	0.0	18.024480175666714	0.0
12	2.810367633558038E-4	0.0	0.0	20.102278646077288	0.0
13	2.810367633558038E-4	0.0	0.0	20.925529004867556	0.0
14	2.810367633558038E-4	0.0	0.0	21.221460716681218	0.0
15	2.810367633558038E-4	0.0	0.0	21.782222738497165	0.0
16	2.810367633558038E-4	0.0	0.0	23.147780371643016	0.0
17	2.810367633558038E-4	0.0	0.0	24.917468870494513	0.0
18	2.810367633558038E-4	0.0	0.0	26.750859035706657	0.0
19	2.810367633558038E-4	0.0	0.0	27.852335790219172	0.0
20	2.810367633558038E-4	0.0	0.0	28.835308709516653	0.0
21	2.810367633558038E-4	0.0	0.0	30.15899186492249	0.0
22	2.810367633558038E-4	0.0	0.0	31.50450220894896	0.0
23	2.810367633558038E-4	0.0	0.0	32.9536214397326	0.0
24	2.810367633558038E-4	0.0	0.0	34.00432421899883	0.0
25	2.810367633558038E-4	0.0	0.0	34.945891055161894	0.0
26	2.810367633558038E-4	0.0	0.0	35.808954955427566	0.0
27	2.810367633558038E-4	0.0	0.0	36.646444510227866	0.0
28	2.810367633558038E-4	0.0	0.0	37.54538743728196	0.0
29	2.810367633558038E-4	0.0	0.0	38.42765551637695	0.0
30	2.810367633558038E-4	0.0	0.0	39.37343790399035	0.0
31	2.810367633558038E-4	0.0	0.0	40.318096144550324	0.0
32	2.810367633558038E-4	0.0	0.0	41.179848539920336	0.0
33	2.810367633558038E-4	0.0	0.0	42.00722077123982	0.0
34	2.810367633558038E-4	0.0	0.0	42.77744886067696	0.0
35	2.810367633558038E-4	0.0	0.0	43.57971514113666	0.0
36	2.810367633558038E-4	0.0	0.0	44.36680543637515	0.0
37	2.810367633558038E-4	0.0	0.0	45.147712922819814	0.0
38	2.810367633558038E-4	0.0	0.0	45.930962382292435	0.0
39	2.810367633558038E-4	0.0	0.0	46.68891853306304	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	25	3.8906175E-5	45.000004	1
CTCGGTG	25	3.8906175E-5	45.000004	14
TTGACGT	50	2.1827873E-11	45.000004	1
CATGTCG	25	3.8906175E-5	45.000004	18
ACGGTAC	25	3.8906175E-5	45.000004	42
TATTTCG	20	7.0330605E-4	45.0	19
TTTAGCG	80	0.0	45.0	1
TCGCAAA	20	7.0330605E-4	45.0	31
TCACCGC	20	7.0330605E-4	45.0	1
ATCTACG	20	7.0330605E-4	45.0	38
GGCGTCG	20	7.0330605E-4	45.0	13
ATTACCG	20	7.0330605E-4	45.0	1
GCGTTAA	20	7.0330605E-4	45.0	9
TCTAGCG	20	7.0330605E-4	45.0	1
CGTTAAT	20	7.0330605E-4	45.0	36
TCTACGC	20	7.0330605E-4	45.0	39
GTTAACG	20	7.0330605E-4	45.0	1
AGACCGT	20	7.0330605E-4	45.0	26
TATTACG	20	7.0330605E-4	45.0	1
GACATCG	20	7.0330605E-4	45.0	27
>>END_MODULE