SRR2934829.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934829.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1116150
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	4774	0.4277202884916902	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	3415	0.3059624602427989	TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2844	0.25480446176589167	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	2807	0.25148949513954216	TruSeq Adapter, Index 13 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1371	0.12283295256013976	TruSeq Adapter, Index 19 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1220	0.10930430497692963	TruSeq Adapter, Index 13 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	30	2.165445E-6	45.000004	1
ATCGTCC	30	2.165445E-6	45.000004	11
CGTACCG	30	2.165445E-6	45.000004	37
ACCGTAC	30	2.165445E-6	45.000004	35
GCTACGA	60	0.0	45.000004	10
ATACGAG	30	2.165445E-6	45.000004	1
ATCGCAC	30	2.165445E-6	45.000004	30
GCGTAAG	125	0.0	45.000004	1
TGCGTAG	60	0.0	45.000004	1
TCACGCG	20	7.0331997E-4	45.0	1
ACACGAA	20	7.0331997E-4	45.0	27
TCCGCAA	20	7.0331997E-4	45.0	32
CGAAAGT	20	7.0331997E-4	45.0	17
CTATCGT	20	7.0331997E-4	45.0	20
ACGTTAC	20	7.0331997E-4	45.0	14
GCACGTC	20	7.0331997E-4	45.0	3
GCACGTA	20	7.0331997E-4	45.0	12
CAACCGG	35	1.2118835E-7	45.0	2
ACCGAAC	20	7.0331997E-4	45.0	11
CGCGACA	20	7.0331997E-4	45.0	34