##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934829.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1116150
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26305604085472	33.0	31.0	34.0	30.0	34.0
2	32.43819110334633	34.0	31.0	34.0	31.0	34.0
3	32.42990010303274	34.0	31.0	34.0	30.0	34.0
4	35.97209962818618	37.0	35.0	37.0	35.0	37.0
5	35.86931774403082	37.0	35.0	37.0	35.0	37.0
6	35.792466962325854	37.0	35.0	37.0	35.0	37.0
7	36.07707655781033	37.0	35.0	37.0	35.0	37.0
8	35.86924786095059	37.0	35.0	37.0	35.0	37.0
9	37.502809658200064	39.0	38.0	39.0	35.0	39.0
10	37.32119159611163	39.0	37.0	39.0	34.0	39.0
11	37.2387716704744	39.0	37.0	39.0	34.0	39.0
12	37.09945616628589	39.0	37.0	39.0	34.0	39.0
13	37.029036419836046	39.0	37.0	39.0	33.0	39.0
14	38.176478071943734	40.0	37.0	41.0	33.0	41.0
15	38.23383505801191	40.0	37.0	41.0	33.0	41.0
16	38.312527886036825	40.0	37.0	41.0	34.0	41.0
17	38.30582448595619	40.0	37.0	41.0	34.0	41.0
18	38.266639788558884	40.0	37.0	41.0	34.0	41.0
19	38.218388209470056	40.0	37.0	41.0	34.0	41.0
20	38.17870895488958	40.0	37.0	41.0	34.0	41.0
21	38.07089817676835	40.0	36.0	41.0	34.0	41.0
22	38.02313219549344	40.0	36.0	41.0	34.0	41.0
23	37.8518666845854	40.0	36.0	41.0	33.0	41.0
24	37.59415938717914	39.0	35.0	41.0	33.0	41.0
25	37.52739327151369	39.0	35.0	41.0	33.0	41.0
26	37.68520001791874	39.0	36.0	41.0	33.0	41.0
27	37.75036151054966	40.0	36.0	41.0	33.0	41.0
28	37.67646284101599	40.0	36.0	41.0	33.0	41.0
29	37.66887963087399	40.0	36.0	41.0	33.0	41.0
30	37.52465349639385	40.0	36.0	41.0	33.0	41.0
31	37.431148143170724	40.0	35.0	41.0	33.0	41.0
32	37.298856784482375	39.0	35.0	41.0	33.0	41.0
33	37.16481745285132	39.0	35.0	41.0	32.0	41.0
34	37.08692648837522	39.0	35.0	41.0	32.0	41.0
35	36.996371455449534	39.0	35.0	41.0	32.0	41.0
36	36.970572055727274	39.0	35.0	41.0	32.0	41.0
37	36.900890561304486	39.0	35.0	41.0	31.0	41.0
38	36.79239170362406	39.0	35.0	41.0	31.0	41.0
39	36.78770953724858	39.0	35.0	41.0	31.0	41.0
40	36.709010437665185	39.0	35.0	41.0	31.0	41.0
41	36.66079021636877	39.0	35.0	41.0	31.0	41.0
42	36.585682031984945	39.0	35.0	41.0	31.0	41.0
43	36.5313228508713	39.0	35.0	41.0	31.0	41.0
44	36.45200734668279	39.0	35.0	41.0	31.0	41.0
45	36.3887425525243	39.0	35.0	41.0	31.0	41.0
46	36.332717824665146	39.0	35.0	41.0	31.0	41.0
47	36.2256184204632	39.0	35.0	40.0	30.0	41.0
48	36.18192088876943	38.0	35.0	40.0	30.0	41.0
49	36.16964028132419	39.0	35.0	40.0	30.0	41.0
50	36.05571025399812	38.0	35.0	40.0	30.0	41.0
51	35.15964520897729	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	21.0
11	25.0
12	17.0
13	24.0
14	39.0
15	59.0
16	88.0
17	145.0
18	293.0
19	540.0
20	925.0
21	1424.0
22	2249.0
23	3304.0
24	4629.0
25	6572.0
26	8941.0
27	10493.0
28	11873.0
29	13506.0
30	15717.0
31	20096.0
32	26565.0
33	36932.0
34	69174.0
35	95820.0
36	83864.0
37	128180.0
38	231025.0
39	343368.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81109169914438	21.01509653720378	22.293060968507817	11.880750795144023
2	34.873538502889396	23.36675178067464	22.927294718451822	18.83241499798414
3	32.6585136406397	24.132867446131794	25.436097298750166	17.77252161447834
4	29.09868745240335	26.809479012677507	24.821932535949472	19.26990099896967
5	25.620301930744077	29.21023159969538	24.761277606056534	20.40818886350401
6	26.009676118801234	34.409353581507865	24.7139721363616	14.866998163329301
7	79.4877928593827	6.030013887022354	9.110872194597501	5.371321058997446
8	79.36352640774089	5.404829100031358	9.31863996774627	5.913004524481477
9	73.37741343009452	7.428033866415805	12.0177395511356	7.1768131523540735
10	38.8048201406621	27.176096402813243	18.392599560990906	15.626483895533754
11	30.547775836581103	26.164404425928417	24.53505353223133	18.75276620525915
12	27.766250055996057	23.531424987680868	28.99359405097881	19.70873090534426
13	25.718944586301124	25.129059714196124	29.891054069793487	19.26094162970927
14	21.36737893652287	28.46606638892622	29.009004166106706	21.157550508444206
15	19.801191596111632	25.81463065000224	34.68404784303185	19.700129910854276
16	23.055592886260808	25.746808224700978	31.13999014469381	20.0576087443444
17	22.472606728486312	24.547775836581103	28.201227433588677	24.778390001343904
18	23.231196523764726	24.066030551449177	31.209246069076734	21.49352685570936
19	24.248264122205796	26.05277068494378	28.022488016843617	21.67647717600681
20	25.981543699323566	26.039869193208798	28.25471486807329	19.723872239394346
21	24.551180396900058	27.043856112529678	29.127178246651436	19.277785243918828
22	22.594095775657394	25.267302781884155	27.610715405635446	24.527886036823006
23	21.308963848945034	27.110334632441873	28.30641042870582	23.27429108990727
24	22.607803610625812	24.611835326792995	29.617166151502932	23.16319491107826
25	21.455091161582224	26.163687676387582	28.07140617300542	24.309814989024773
26	20.42521166509878	27.703176096402814	28.449312368409263	23.422299870089144
27	21.48940554584957	27.040899520673744	28.548850960892352	22.92084397258433
28	20.46624557631143	26.621063477131212	29.347757917842582	23.56493302871478
29	21.051650763786228	26.392957935761324	28.732697218115845	23.822694082336604
30	22.895847332347802	26.61076020248175	28.48264122205797	22.010751243112484
31	23.597903507593067	27.273036778210813	25.28092102315997	23.84813869103615
32	22.213860144245846	29.123146530484256	25.70998521704072	22.95300810822918
33	22.480490973435472	27.828607266048472	25.989159163194913	23.70174259732115
34	21.435470142901938	26.621153070823816	27.72369305200914	24.219683734265107
35	19.78381041974645	27.982350042557	27.12888052681091	25.104959010885636
36	22.212785019934596	28.3658110469023	25.93629888455853	23.48510504860458
37	21.926533172064687	28.04694709492452	27.59817228867088	22.42834744433992
38	21.2948976392062	30.849796174349326	24.995027550060474	22.860278636384
39	21.690095417282624	27.771715271244908	25.752811002105453	24.78537830936702
40	22.27854679030596	25.492989293553737	28.514088608161988	23.71437530797832
41	19.528199614747123	26.57402678851409	26.64614971106034	27.25162388567845
42	22.42440532186534	25.82305245710702	26.79666711463513	24.95587510639251
43	23.438068359987458	24.57913362899252	27.070644626618286	24.91215338440174
44	21.229404649912645	26.71424091743941	26.754647672803834	25.301706759844105
45	22.032880885185683	25.502844599740175	26.53388881422748	25.93038570084666
46	22.471800385252877	26.725440129014917	26.34439815436993	24.458361331362273
47	20.220758858576357	26.331048694171933	28.615419074497154	24.83277337275456
48	20.252743806835998	25.60865475070555	28.436410876674284	25.702190565784168
49	21.639206199883528	23.781928952201763	29.822246113873586	24.756618734041126
50	20.69488867983694	24.865654257940243	28.996371455449538	25.443085606773288
51	19.972853111140974	24.47117322940465	26.701876987860057	28.85409667159432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	462.0
1	873.5
2	1285.0
3	3678.5
4	6072.0
5	4348.5
6	2625.0
7	2636.5
8	2648.0
9	2678.5
10	2709.0
11	2785.5
12	2862.0
13	2794.0
14	2726.0
15	2861.0
16	2996.0
17	2932.0
18	2868.0
19	2849.0
20	2830.0
21	3169.0
22	3508.0
23	3808.0
24	4108.0
25	5140.0
26	6998.5
27	7825.0
28	9493.0
29	11161.0
30	12850.0
31	14539.0
32	16408.5
33	18278.0
34	20734.0
35	23190.0
36	26061.5
37	28933.0
38	33434.5
39	37936.0
40	43015.0
41	48094.0
42	54401.5
43	60709.0
44	68239.5
45	75770.0
46	87356.0
47	98942.0
48	111287.0
49	123632.0
50	122749.0
51	121866.0
52	107732.0
53	93598.0
54	80262.5
55	66927.0
56	60562.5
57	54198.0
58	50100.5
59	46003.0
60	42295.5
61	38588.0
62	35555.0
63	32522.0
64	28688.5
65	24855.0
66	19723.0
67	14591.0
68	12710.5
69	10830.0
70	8684.5
71	6539.0
72	6007.0
73	5475.0
74	4383.0
75	2606.0
76	1921.0
77	1359.5
78	798.0
79	699.5
80	601.0
81	502.5
82	404.0
83	291.5
84	179.0
85	117.5
86	56.0
87	35.5
88	15.0
89	10.0
90	5.0
91	4.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1116150.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.421017473636134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24791502701669	17.399465778349168
2	11.562985019277091	5.647597184063175
3	4.786652210010195	3.50684751782634
4	2.5979767369206774	2.537809411537602
5	1.5888745218260416	1.9400966230464507
6	1.0011138195799936	1.466893084863698
7	0.6784800489517733	1.1598421191675328
8	0.5143243574586881	1.004825929649224
9	0.370678842268993	0.814711903574246
>10	3.6252763430527843	21.367767335024475
>50	1.6365173686498382	28.98665133062529
>100	0.38327495138737283	11.99378943820503
>500	0.0033360488998900317	0.5863103353403336
>1k	0.0025947046999144695	1.587392008727511
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	4774	0.4277202884916902	TruSeq Adapter, Index 19 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	3415	0.3059624602427989	TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2844	0.25480446176589167	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	2807	0.25148949513954216	TruSeq Adapter, Index 13 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1371	0.12283295256013976	TruSeq Adapter, Index 19 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1220	0.10930430497692963	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7918738520808134E-4	0.0	0.0	0.26725798503785336	0.0
2	1.7918738520808134E-4	0.0	0.0	1.3007212292254626	0.0
3	1.7918738520808134E-4	0.0	0.0	1.8734041123504905	0.0
4	1.7918738520808134E-4	0.0	0.0	2.4785199121981814	0.0
5	2.6878107781212203E-4	0.0	0.0	4.129014917349819	0.0
6	2.6878107781212203E-4	0.0	0.0	5.047708641311652	0.0
7	2.6878107781212203E-4	0.0	0.0	5.9202616135824035	0.0
8	2.6878107781212203E-4	0.0	0.0	7.468171840702414	0.0
9	2.6878107781212203E-4	0.0	0.0	8.021860860995385	0.0
10	2.6878107781212203E-4	0.0	0.0	9.192133673789366	0.0
11	2.6878107781212203E-4	0.0	0.0	10.825605877346234	0.0
12	2.6878107781212203E-4	0.0	0.0	12.107870805895265	0.0
13	2.6878107781212203E-4	0.0	0.0	12.639071809344623	0.0
14	2.6878107781212203E-4	0.0	0.0	12.841822335707565	0.0
15	2.6878107781212203E-4	0.0	0.0	13.234511490391077	0.0
16	2.6878107781212203E-4	0.0	0.0	14.092998252922994	0.0
17	2.6878107781212203E-4	0.0	0.0	15.057026385342471	0.0
18	2.6878107781212203E-4	0.0	0.0	16.194597500335977	0.0
19	2.6878107781212203E-4	0.0	0.0	16.808762263136675	0.0
20	2.6878107781212203E-4	0.0	0.0	17.44765488509609	0.0
21	2.6878107781212203E-4	0.0	0.0	18.141378846929175	0.0
22	2.6878107781212203E-4	0.0	0.0	18.88115396676074	0.0
23	2.6878107781212203E-4	0.0	0.0	19.650226224073826	0.0
24	2.6878107781212203E-4	0.0	0.0	20.220758858576357	0.0
25	2.6878107781212203E-4	0.0	0.0	20.72391703624065	0.0
26	2.6878107781212203E-4	0.0	0.0	21.210948349236215	0.0
27	2.6878107781212203E-4	0.0	0.0	21.68230076602607	0.0
28	2.6878107781212203E-4	0.0	0.0	22.169690453792054	0.0
29	2.6878107781212203E-4	0.0	0.0	22.670340008063434	0.0
30	2.6878107781212203E-4	0.0	0.0	23.248936074900328	0.0
31	2.6878107781212203E-4	0.0	0.0	23.756663530887426	0.0
32	2.6878107781212203E-4	0.0	0.0	24.274067105675762	0.0
33	2.6878107781212203E-4	0.0	0.0	24.77247681763204	0.0
34	2.6878107781212203E-4	0.0	0.0	25.273484746673834	0.0
35	2.6878107781212203E-4	0.0	0.0	25.76562290014783	0.0
36	2.6878107781212203E-4	0.0	0.0	26.25632755454016	0.0
37	2.6878107781212203E-4	0.0	0.0	26.74102943152802	0.0
38	2.6878107781212203E-4	0.0	0.0	27.235676208394928	0.0
39	2.6878107781212203E-4	0.0	0.0	27.742149352685573	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	30	2.165445E-6	45.000004	1
ATCGTCC	30	2.165445E-6	45.000004	11
CGTACCG	30	2.165445E-6	45.000004	37
ACCGTAC	30	2.165445E-6	45.000004	35
GCTACGA	60	0.0	45.000004	10
ATACGAG	30	2.165445E-6	45.000004	1
ATCGCAC	30	2.165445E-6	45.000004	30
GCGTAAG	125	0.0	45.000004	1
TGCGTAG	60	0.0	45.000004	1
TCACGCG	20	7.0331997E-4	45.0	1
ACACGAA	20	7.0331997E-4	45.0	27
TCCGCAA	20	7.0331997E-4	45.0	32
CGAAAGT	20	7.0331997E-4	45.0	17
CTATCGT	20	7.0331997E-4	45.0	20
ACGTTAC	20	7.0331997E-4	45.0	14
GCACGTC	20	7.0331997E-4	45.0	3
GCACGTA	20	7.0331997E-4	45.0	12
CAACCGG	35	1.2118835E-7	45.0	2
ACCGAAC	20	7.0331997E-4	45.0	11
CGCGACA	20	7.0331997E-4	45.0	34
>>END_MODULE