SRR2934827.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178121
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3233	1.8150583030636478	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1899	1.0661292043049388	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1577	0.8853532149493883	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	1239	0.6955945677376615	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC	1102	0.6186805598441509	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	955	0.53615239079053	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	572	0.321130018358307	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	568	0.3188843538942629	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.31551585719819675	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	539	0.3026032865299431	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.2655498228732154	No Hit
ACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	444	0.24926875550889566	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.2009869695319474	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	349	0.19593422448784814	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	302	0.16954766703532992	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.15775792859909837	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	261	0.14652960627887784	No Hit
AAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	254	0.14259969346680065	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC	252	0.1414768612347786	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	243	0.13642411619067937	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	240	0.1347398678426463	No Hit
GGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	234	0.13137137114658015	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.12519579387045884	No Hit
AGGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	215	0.12070446494237064	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC	209	0.11733596824630448	No Hit
GAGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	208	0.11677455213029346	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGT	201	0.11284463931821628	No Hit
GACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	194	0.10891472650613908	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	179	0.1004934847659737	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCCA	30	2.155146E-6	45.000004	9
ACAGGGC	30	2.155146E-6	45.000004	4
TATAGTT	30	2.155146E-6	45.000004	4
CGTAAGG	30	2.155146E-6	45.000004	2
ATGGGTG	20	7.017224E-4	45.0	5
AGTTTAG	20	7.017224E-4	45.0	1
TACCATT	35	1.2042074E-7	45.0	4
GGTAACT	20	7.017224E-4	45.0	8
ACGCAGG	35	1.2042074E-7	45.0	2
TGGACGG	20	7.017224E-4	45.0	2
CATTGGT	20	7.017224E-4	45.0	14
ACTCTTA	40	6.7593646E-9	45.0	5
GACAAGG	20	7.017224E-4	45.0	2
AGGGTAT	35	1.2042074E-7	45.0	6
TACGGGG	55	1.8189894E-12	45.0	4
AGTGCAG	20	7.017224E-4	45.0	1
CTACGAA	35	1.2042074E-7	45.0	11
AACGCAG	20	7.017224E-4	45.0	1
CGAATAT	35	1.2042074E-7	45.0	14
TCGTAGG	20	7.017224E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content