##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178121
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58751635124438	31.0	31.0	34.0	30.0	34.0
2	31.677050993425816	31.0	31.0	34.0	30.0	34.0
3	31.722385344793707	31.0	31.0	34.0	30.0	34.0
4	35.535821155282086	37.0	35.0	37.0	33.0	37.0
5	33.276250414044384	37.0	35.0	37.0	30.0	37.0
6	34.154131180489664	37.0	35.0	37.0	28.0	37.0
7	35.33497453977914	37.0	35.0	37.0	32.0	37.0
8	35.22721633047198	37.0	35.0	37.0	33.0	37.0
9	37.267576535052015	39.0	37.0	39.0	34.0	39.0
10	36.8442126419681	39.0	37.0	39.0	32.0	39.0
11	36.639093649822314	39.0	37.0	39.0	32.0	39.0
12	36.91658479348308	39.0	37.0	39.0	33.0	39.0
13	36.7730812200695	39.0	37.0	39.0	33.0	39.0
14	37.642855137799586	39.0	37.0	41.0	33.0	41.0
15	37.838733220675834	39.0	37.0	41.0	33.0	41.0
16	37.838098820464744	39.0	37.0	41.0	33.0	41.0
17	37.845414072456364	39.0	37.0	41.0	33.0	41.0
18	37.75215162726461	39.0	37.0	40.0	33.0	41.0
19	37.54104793932215	39.0	36.0	40.0	33.0	41.0
20	37.36638577146996	39.0	35.0	40.0	33.0	41.0
21	37.247320641586334	39.0	35.0	40.0	33.0	41.0
22	37.175363937997204	39.0	35.0	40.0	33.0	41.0
23	36.94228642327407	39.0	35.0	40.0	32.0	41.0
24	36.63121698171468	38.0	35.0	40.0	31.0	41.0
25	36.45149084049607	38.0	35.0	40.0	31.0	41.0
26	36.71565396556274	38.0	35.0	40.0	32.0	41.0
27	36.56428495236384	38.0	35.0	40.0	31.0	41.0
28	36.64497167655695	38.0	35.0	40.0	31.0	41.0
29	36.677483283835144	38.0	35.0	40.0	32.0	41.0
30	36.36914793876073	38.0	35.0	40.0	31.0	41.0
31	36.256443653471514	38.0	35.0	40.0	31.0	41.0
32	36.03723873097501	38.0	35.0	40.0	30.0	41.0
33	35.89723839412534	38.0	35.0	40.0	30.0	41.0
34	35.8666187591581	38.0	35.0	40.0	30.0	41.0
35	35.61219620370422	38.0	35.0	40.0	27.0	41.0
36	35.32365639088036	38.0	35.0	40.0	26.0	41.0
37	35.264797525277764	38.0	35.0	40.0	25.0	41.0
38	35.199027627287066	38.0	35.0	40.0	25.0	41.0
39	35.2036256252772	38.0	35.0	40.0	24.0	41.0
40	35.13923119677074	38.0	35.0	40.0	24.0	41.0
41	34.96950949073944	38.0	35.0	40.0	23.0	41.0
42	34.97991253136912	38.0	35.0	40.0	23.0	41.0
43	34.83084532424588	38.0	35.0	40.0	23.0	41.0
44	34.732328024208265	38.0	34.0	40.0	22.0	41.0
45	34.734130169940656	38.0	34.0	40.0	23.0	41.0
46	34.59670111890232	38.0	34.0	40.0	22.0	41.0
47	34.500637207291675	38.0	34.0	40.0	22.0	41.0
48	34.5001263186261	38.0	34.0	40.0	22.0	41.0
49	34.505847148848254	38.0	34.0	40.0	22.0	41.0
50	34.39777454651613	38.0	34.0	40.0	22.0	41.0
51	33.40448908326363	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	2.0
12	5.0
13	11.0
14	10.0
15	11.0
16	37.0
17	43.0
18	83.0
19	182.0
20	313.0
21	484.0
22	672.0
23	1062.0
24	1506.0
25	2240.0
26	2823.0
27	3271.0
28	3281.0
29	3312.0
30	3719.0
31	4435.0
32	5612.0
33	7826.0
34	11909.0
35	16105.0
36	19270.0
37	25602.0
38	37759.0
39	26523.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.365257325076776	21.306864434850468	20.556812503859735	17.771065736213025
2	35.240651018128126	24.32335322617771	24.9184543091494	15.517541446544763
3	27.098994503736222	22.72949287282241	35.979474626798634	14.192037996642732
4	24.74890664211407	25.898125431588635	32.80971923580039	16.54324869049691
5	18.110161070283684	32.87259784079362	32.013631183296745	17.003609905625954
6	20.81506391722481	32.28984791237417	33.3043268340061	13.590761336394921
7	64.1401070059117	8.537454876179677	21.005945396668558	6.316492721240056
8	59.92331055855289	8.767074067628185	22.132146125386676	9.177469248432244
9	55.093447712510034	9.94043375009123	25.629768528135365	9.336350009263365
10	30.752129170619973	23.79225358043128	30.902588689710925	14.553028559237822
11	21.349532059667307	23.83997395029222	34.128485692310285	20.682008297730196
12	21.318092757170685	19.892657238618693	40.72344080709181	18.065809197118814
13	20.294069761566575	23.879834494529	40.49719011233937	15.32890563156506
14	14.051122551523964	27.526793584136627	37.68224970666008	20.73983415767933
15	12.429191392368109	25.44618545819976	43.21388269771672	18.91074045171541
16	11.822861987076202	25.919459243997057	39.79317430286154	22.4645044660652
17	11.87170518916916	26.16985083173798	36.56783871637819	25.390605262714672
18	12.296697188989507	25.280006287860502	43.05331768853757	19.369978834612425
19	14.646223634495653	26.946850736297236	38.02078362461473	20.386142004592383
20	15.07739121159212	29.437292626922147	37.99832697997429	17.486989181511444
21	14.63387247994341	28.903385900595662	38.19033129165006	18.27241032781087
22	11.651068655576827	29.2464111474784	33.280747357133635	25.821772839811143
23	13.088855328681065	27.1332408868129	35.10085840524138	24.677045379264655
24	16.437141044570826	25.863317632395955	34.589969739671346	23.10957158336187
25	13.625007719471597	28.97356291509704	32.34767377232331	25.053755593108058
26	14.181932506554535	29.331184980996067	35.7178547167375	20.769027795711903
27	17.415127918662034	25.835246826595405	37.217958578718964	19.5316666760236
28	15.051004654139602	25.52366088220929	36.00249268755509	23.422841776096025
29	18.194373487685336	22.76317783978307	37.31732923125291	21.725119441278682
30	19.636090073601654	25.472010599536272	36.05526580246013	18.836633524401954
31	20.781378950264145	27.636269726758776	29.091460299459353	22.49089102351772
32	21.799787784708148	25.87342312248415	31.87215432206197	20.454634770745727
33	19.395803975948933	22.568927863643253	36.46846806384424	21.566800096563572
34	20.28564851982641	21.371427288191736	32.86810651186553	25.474817680116324
35	23.62326732951196	20.068941899046152	33.68889687347365	22.618893897968235
36	23.756322949006574	21.3747957848878	37.44084077677534	17.428040489330286
37	22.378046384199504	22.09733832619399	37.47901707266409	18.045598216942416
38	20.025712858113305	24.420478214247616	36.03224774170367	19.521561185935404
39	21.114298707058683	19.181904435748734	39.09477265454382	20.60902420264876
40	23.69569000847738	19.203799664273163	34.650602680200535	22.449907647048917
41	23.38859539301935	20.813941084992784	31.361827072607944	24.435636449379917
42	23.95730991853852	19.801707827824906	36.897951392592674	19.343030861043896
43	24.451917516744235	18.64238354826214	38.13250543170093	18.773193503292706
44	21.155282083527492	19.820795975769283	34.35136789036666	24.672554050336572
45	22.80696829683193	18.09668708349942	33.385732170827694	25.71061244884096
46	23.91127379702562	20.454634770745727	35.20584321893544	20.42824821329321
47	19.10948175678331	21.125527029378905	39.256460495955	20.508530717882785
48	19.985852313876524	18.36504398695269	38.055591423807414	23.593512275363377
49	22.587454595471616	16.421421393322515	39.81563094750198	21.175493063703886
50	20.005501877936908	16.206960437006305	36.53471516553354	27.252822519523246
51	19.65180972484996	16.191802201874005	31.80590722037267	32.35048085290336
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1485.0
1	1994.0
2	2503.0
3	4122.0
4	5741.0
5	3858.0
6	1975.0
7	1921.0
8	1867.0
9	2027.5
10	2188.0
11	2129.0
12	2070.0
13	2002.0
14	1934.0
15	1828.0
16	1722.0
17	1597.0
18	1472.0
19	1364.0
20	1256.0
21	1320.0
22	1384.0
23	1479.5
24	1575.0
25	1876.5
26	2262.5
27	2347.0
28	2187.5
29	2028.0
30	2113.0
31	2198.0
32	2257.0
33	2316.0
34	2500.0
35	2684.0
36	2746.5
37	2809.0
38	3036.5
39	3264.0
40	4010.5
41	4757.0
42	6164.5
43	7572.0
44	9056.5
45	10541.0
46	13828.5
47	17116.0
48	21541.0
49	25966.0
50	26201.0
51	26436.0
52	20523.5
53	14611.0
54	11046.5
55	7482.0
56	5669.5
57	3857.0
58	3313.5
59	2770.0
60	2678.0
61	2586.0
62	2458.0
63	2330.0
64	1973.0
65	1616.0
66	1425.5
67	1235.0
68	990.5
69	746.0
70	672.0
71	598.0
72	500.5
73	403.0
74	332.5
75	193.5
76	125.0
77	85.0
78	45.0
79	44.5
80	44.0
81	27.0
82	10.0
83	7.0
84	4.0
85	5.0
86	6.0
87	6.5
88	7.0
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	178121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.713099522234884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19148013301613	30.835218755789604
2	11.085553554764003	9.469967044873991
3	4.902669523271251	6.282246338163383
4	2.835136236379648	4.843898248943134
5	1.9689541409813227	4.205006708922586
6	1.4405699189022225	3.691872378888508
7	1.164548310353439	3.481902751500385
8	0.8950986448653409	3.058595000028071
9	0.6992547416569183	2.6880603634607936
>10	2.656379385129007	17.444882972810618
>50	0.09857914591027983	2.8890473329927406
>100	0.048632378649071384	4.234200346955159
>500	0.006571943060685322	1.7942859067712398
>1k	0.006571943060685322	5.080815849899787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3233	1.8150583030636478	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1899	1.0661292043049388	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1577	0.8853532149493883	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	1239	0.6955945677376615	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC	1102	0.6186805598441509	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	955	0.53615239079053	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	572	0.321130018358307	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	568	0.3188843538942629	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	562	0.31551585719819675	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	539	0.3026032865299431	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	473	0.2655498228732154	No Hit
ACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	444	0.24926875550889566	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.2009869695319474	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	349	0.19593422448784814	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	302	0.16954766703532992	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.15775792859909837	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	261	0.14652960627887784	No Hit
AAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	254	0.14259969346680065	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC	252	0.1414768612347786	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	243	0.13642411619067937	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	240	0.1347398678426463	No Hit
GGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	234	0.13137137114658015	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.12519579387045884	No Hit
AGGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	215	0.12070446494237064	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC	209	0.11733596824630448	No Hit
GAGCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	208	0.11677455213029346	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGT	201	0.11284463931821628	No Hit
GACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	194	0.10891472650613908	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	179	0.1004934847659737	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7382621925544994	0.0
2	0.0	0.0	0.0	3.420146978739172	0.0
3	0.0	0.0	0.0	4.423958994166886	0.0
4	0.0	0.0	0.0	5.712408980412191	0.0
5	0.0	0.0	0.0	9.254944672441766	0.0
6	0.0	0.0	0.0	11.201935762768006	0.0
7	0.0	0.0	0.0	13.271315566384649	0.0
8	0.0	0.0	0.0	16.380999432969723	0.0
9	0.0	0.0	0.0	17.651484103502675	0.0
10	0.0	0.0	0.0	21.310794347662544	0.0
11	0.0	0.0	0.0	26.85646274161946	0.0
12	0.0	0.0	0.0	30.300750613347105	0.0
13	0.0	0.0	0.0	31.72057197073899	0.0
14	0.0	0.0	0.0	32.296023489650295	0.0
15	0.0	0.0	0.0	33.42222421836841	0.0
16	0.0	0.0	0.0	35.55672829144233	0.0
17	0.0	0.0	0.0	38.03762610809506	0.0
18	0.0	0.0	0.0	40.5039271057315	0.0
19	0.0	0.0	0.0	41.93216970486355	0.0
20	0.0	0.0	0.0	43.23746217458918	0.0
21	0.0	0.0	0.0	44.747110110542835	0.0
22	0.0	0.0	0.0	46.2528281336844	0.0
23	0.0	0.0	0.0	47.65580700759596	0.0
24	0.0	0.0	0.0	48.562494034953765	0.0
25	0.0	0.0	0.0	49.21485956175858	0.0
26	0.0	0.0	0.0	49.73248522072075	0.0
27	0.0	0.0	0.0	50.21642591272225	0.0
28	0.0	0.0	0.0	50.64927773816675	0.0
29	0.0	0.0	0.0	51.11806019503596	0.0
30	0.0	0.0	0.0	51.52957820807204	0.0
31	0.0	0.0	0.0	51.890007354551116	0.0
32	0.0	0.0	0.0	52.20776887621336	0.0
33	0.0	0.0	0.0	52.49016118256691	0.0
34	0.0	0.0	0.0	52.8157825298533	0.0
35	0.0	0.0	0.0	53.11221023910712	0.0
36	0.0	0.0	0.0	53.351934920643835	0.0
37	0.0	0.0	0.0	53.599519427804694	0.0
38	0.0	0.0	0.0	53.81566463246894	0.0
39	0.0	0.0	0.0	54.03630116606127	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCCA	30	2.155146E-6	45.000004	9
ACAGGGC	30	2.155146E-6	45.000004	4
TATAGTT	30	2.155146E-6	45.000004	4
CGTAAGG	30	2.155146E-6	45.000004	2
ATGGGTG	20	7.017224E-4	45.0	5
AGTTTAG	20	7.017224E-4	45.0	1
TACCATT	35	1.2042074E-7	45.0	4
GGTAACT	20	7.017224E-4	45.0	8
ACGCAGG	35	1.2042074E-7	45.0	2
TGGACGG	20	7.017224E-4	45.0	2
CATTGGT	20	7.017224E-4	45.0	14
ACTCTTA	40	6.7593646E-9	45.0	5
GACAAGG	20	7.017224E-4	45.0	2
AGGGTAT	35	1.2042074E-7	45.0	6
TACGGGG	55	1.8189894E-12	45.0	4
AGTGCAG	20	7.017224E-4	45.0	1
CTACGAA	35	1.2042074E-7	45.0	11
AACGCAG	20	7.017224E-4	45.0	1
CGAATAT	35	1.2042074E-7	45.0	14
TCGTAGG	20	7.017224E-4	45.0	2
>>END_MODULE