FastQCFastQC Report
Sat 14 Jan 2017
SRR2934826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188301
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37351.983526375324613No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC10100.5363752715067897No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC10100.5363752715067897No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC9390.4986696831137381No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8950.4753028396025512No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT6800.3611239451728881No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC5950.3159834520262771No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.2522556969957674No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.23791695211390274No Hit
ACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC4100.21773649635424136No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.16409896920356237No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.15453980594898595No Hit
ACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG2800.14869809507118922No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCG2750.14604277194491797No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.13011083318729058No Hit
GACAGATTATTTTTATTTATTCAATGTCATATTTAAAATATTTATTTTTTA2280.12108273455796836No Hit
GCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG2180.11577208830542589No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGC2130.11311676517915464No Hit
AGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG1930.10249547267406972No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG253.878348E-545.0000041
TGGGTAC253.878348E-545.0000046
GATGCTA253.878348E-545.0000049
CAATCTA253.878348E-545.00000410
GACTACT253.878348E-545.0000049
ACGAAGG253.878348E-545.0000042
GATCGAG207.018252E-445.01
CCCTAGC207.018252E-445.021
ACACAGG207.018252E-445.02
GCCGGGA207.018252E-445.04
ACGGGTA207.018252E-445.05
TACGAAG207.018252E-445.01
ACTACTC207.018252E-445.010
CGGGCAA207.018252E-445.06
AGATTCC207.018252E-445.022
GATGCGG207.018252E-445.02
CTATTAG207.018252E-445.019
GGCGATA351.2046985E-745.08
CTCTATT207.018252E-445.017
AGTACGG207.018252E-445.02
Produced by FastQC (version 0.11.5)