##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934826.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188301
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.744791583687817	31.0	31.0	34.0	30.0	34.0
2	31.844477724494293	33.0	31.0	34.0	30.0	34.0
3	31.853516444416123	33.0	31.0	34.0	30.0	34.0
4	35.637585567787745	37.0	35.0	37.0	33.0	37.0
5	33.04561845130934	37.0	35.0	37.0	28.0	37.0
6	34.10257513236786	37.0	35.0	37.0	28.0	37.0
7	35.42438967397943	37.0	35.0	37.0	32.0	37.0
8	35.44601993616603	37.0	35.0	37.0	33.0	37.0
9	37.47526566507879	39.0	37.0	39.0	35.0	39.0
10	36.86838625392324	39.0	37.0	39.0	32.0	39.0
11	36.618759326822484	39.0	35.0	39.0	32.0	39.0
12	36.919819862879116	39.0	37.0	39.0	33.0	39.0
13	36.87082384055316	39.0	37.0	39.0	33.0	39.0
14	37.80344767154715	40.0	37.0	41.0	33.0	41.0
15	37.98192256015635	40.0	37.0	41.0	33.0	41.0
16	37.99381840776204	40.0	37.0	41.0	33.0	41.0
17	37.93027652535037	40.0	37.0	41.0	33.0	41.0
18	37.84430247316796	39.0	37.0	41.0	33.0	41.0
19	37.64785104699391	39.0	36.0	41.0	33.0	41.0
20	37.4698594271937	39.0	35.0	41.0	33.0	41.0
21	37.37322159733618	39.0	35.0	40.0	33.0	41.0
22	37.3684526370014	39.0	35.0	40.0	33.0	41.0
23	37.11454532902109	39.0	35.0	40.0	32.0	41.0
24	36.84411128990287	39.0	35.0	40.0	32.0	41.0
25	36.653915805014314	38.0	35.0	40.0	31.0	41.0
26	36.9244985422276	39.0	35.0	40.0	32.0	41.0
27	36.85300662237588	39.0	35.0	40.0	32.0	41.0
28	36.92568812698817	39.0	35.0	40.0	32.0	41.0
29	37.02406253817027	39.0	35.0	40.0	33.0	41.0
30	36.75280534888291	39.0	35.0	40.0	32.0	41.0
31	36.611228830436374	39.0	35.0	40.0	31.0	41.0
32	36.39851089479079	39.0	35.0	40.0	31.0	41.0
33	36.21320120445457	39.0	35.0	40.0	30.0	41.0
34	36.037211698291564	39.0	35.0	41.0	30.0	41.0
35	35.86759496763161	39.0	35.0	41.0	29.0	41.0
36	35.72013425313727	39.0	35.0	41.0	27.0	41.0
37	35.662311936739584	39.0	35.0	40.0	27.0	41.0
38	35.55875964546126	39.0	35.0	40.0	26.0	41.0
39	35.47044359828148	39.0	35.0	40.0	25.0	41.0
40	35.30937169744186	39.0	35.0	40.0	24.0	41.0
41	35.19308447644994	38.0	35.0	40.0	24.0	41.0
42	35.31892023940393	39.0	35.0	40.0	24.0	41.0
43	35.24782130737489	39.0	35.0	40.0	23.0	41.0
44	35.15036032734824	39.0	35.0	40.0	23.0	41.0
45	35.09478441431538	39.0	35.0	40.0	23.0	41.0
46	34.9851620543704	38.0	35.0	40.0	23.0	41.0
47	34.92025533587182	38.0	35.0	40.0	23.0	41.0
48	34.93036149569041	38.0	35.0	40.0	23.0	41.0
49	34.951731536210644	38.0	35.0	40.0	23.0	41.0
50	34.85948030015773	38.0	35.0	40.0	22.0	41.0
51	33.98445573841881	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	9.0
11	1.0
12	7.0
13	4.0
14	10.0
15	10.0
16	19.0
17	41.0
18	86.0
19	189.0
20	301.0
21	487.0
22	750.0
23	1056.0
24	1611.0
25	2208.0
26	2965.0
27	3346.0
28	3337.0
29	3318.0
30	3636.0
31	4171.0
32	5429.0
33	7473.0
34	12081.0
35	16208.0
36	18237.0
37	24175.0
38	38787.0
39	38339.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.92971890749386	23.53359780351671	24.87931556391097	17.657367725078466
2	36.059288054763385	23.21867648074094	25.24628121996166	15.475754244534018
3	26.81876357533948	23.055108576162635	35.02583629401862	15.100291554479265
4	23.921806044577565	25.772035198963362	33.82403704706826	16.482121709390814
5	18.641430475674582	33.168703299504514	32.01363773957653	16.17622848524437
6	22.10556502620804	31.716241549434148	33.200036112394514	12.978157311963292
7	68.42289738238246	6.929331230317418	19.412005246918497	5.235766140381623
8	66.75376126520837	6.071130795906554	20.110886293753087	7.064221645131996
9	61.56632200572487	8.727516051428298	22.419955284358554	7.286206658488272
10	31.886713294140762	25.958438882427604	29.03808264427698	13.116765179154651
11	21.44545169701701	23.721594680856715	36.459710782205086	18.37324283992119
12	20.09495435499546	20.148060817520886	41.81390433401841	17.94308049346525
13	19.251623730091715	23.278686783394672	42.826113509753	14.643575976760612
14	15.355733639226557	26.99985661255118	39.22337109202819	18.42103865619407
15	12.894780165798375	25.134757648658262	44.294507198580995	17.675954986962363
16	14.988236918550617	25.426843192548098	40.46181379812109	19.123106090780187
17	14.138002453518569	25.84319785874743	36.867568414400345	23.151231273333654
18	14.384416439636539	25.13422658403301	41.25416221900043	19.227194757330018
19	15.134279690495536	26.623331793245917	38.74753718780038	19.49485132845816
20	16.684457331612684	28.475685206132734	37.9371325696624	16.90272489259218
21	16.162951869613014	27.16076919400322	39.4750957243987	17.201183211985065
22	14.218724276557214	25.98446104906506	36.45758652370407	23.339228150673655
23	13.645174481282627	25.952066106924555	38.150620549014604	22.25213886277821
24	16.398744563225897	24.773633703485377	37.684876872666635	21.14274486062209
25	13.803431739608394	27.23830462929034	35.920680187572025	23.037583443529243
26	13.379111104030251	28.133679587469	37.94934705604325	20.5378622524575
27	15.337146377342659	26.40293997376541	37.956250896171554	20.303662752720378
28	11.977631557984292	26.04234709321777	39.45810165639057	22.52191969240737
29	15.105071136106552	23.782136048135698	39.46659869039463	21.646194125363117
30	16.517171974657597	25.50650288633624	34.80916192691489	23.16716321209128
31	15.432738009888421	29.456561568977328	33.63019845885046	21.48050196228379
32	17.095501351559474	27.37956781960797	32.28129431070467	23.243636518127893
33	16.37484665508946	25.537835699226243	32.535674266201454	25.551643379482847
34	15.22084322441198	25.784780749969467	35.160195644207946	23.834180381410615
35	15.42477204050961	23.803378633145865	33.7284454145225	27.04340391182203
36	16.250577532779964	24.760357087854022	34.885104168326244	24.10396121103977
37	17.171974657596085	26.296195984089305	35.31367332090642	21.218156037408193
38	17.92555536083186	26.22344013042947	31.43955687967669	24.411447629061982
39	19.743389573077145	24.329663676772824	32.60099521510772	23.3259515350423
40	18.65098963892916	25.530931859097933	32.427337082649586	23.39074141932332
41	15.118878816363162	25.73804706294709	31.37476699539567	27.76830712529408
42	17.886256578563046	21.588308081210403	36.153817558058634	24.371617782167913
43	19.586725508627147	21.79436115580905	35.05929336540964	23.55961997015417
44	18.449185081332548	22.241517570273125	33.1097551261013	26.199542222293033
45	20.2813580384597	21.665843516497524	30.756607771599725	27.29619067344305
46	20.149122946771392	22.971731429997718	33.022129462934345	23.857016160296546
47	15.312186339955709	24.388080785550795	36.71568393157764	23.584048942915864
48	16.253232855906237	21.10875672460582	36.19364740495271	26.444363014535238
49	19.373237529274938	18.865008682906623	38.12778477012868	23.63396901768976
50	18.057259387894913	19.70621504930935	35.63443635455998	26.602089208235753
51	16.84536991306472	19.447055512185276	31.944068273668226	31.76350630108178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1194.0
1	1882.5
2	2571.0
3	4459.5
4	6348.0
5	4127.5
6	1907.0
7	1871.5
8	1836.0
9	2034.5
10	2233.0
11	2489.0
12	2745.0
13	2596.5
14	2448.0
15	2289.5
16	2131.0
17	2055.0
18	1979.0
19	1818.5
20	1658.0
21	2013.0
22	2368.0
23	2247.0
24	2126.0
25	1957.5
26	2103.5
27	2418.0
28	2450.5
29	2483.0
30	2734.0
31	2985.0
32	3428.5
33	3872.0
34	3745.5
35	3619.0
36	4017.5
37	4416.0
38	4550.5
39	4685.0
40	5437.0
41	6189.0
42	7630.5
43	9072.0
44	10472.0
45	11872.0
46	14664.5
47	17457.0
48	20460.5
49	23464.0
50	22784.5
51	22105.0
52	17569.0
53	13033.0
54	10544.0
55	8055.0
56	6598.5
57	5142.0
58	4626.0
59	4110.0
60	3636.5
61	3163.0
62	2799.0
63	2435.0
64	2093.5
65	1752.0
66	1463.0
67	1174.0
68	916.5
69	659.0
70	509.0
71	359.0
72	289.5
73	220.0
74	166.0
75	87.5
76	63.0
77	44.5
78	26.0
79	20.0
80	14.0
81	10.5
82	7.0
83	4.0
84	1.0
85	2.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	188301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.53029989219388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.85123612121501	32.88617691886926
2	10.215739824211994	9.098199159855763
3	4.339840908277778	5.797632513900616
4	2.542605335654912	4.52891912416822
5	1.776961515068395	3.956431458144142
6	1.2677248929649019	3.3871301798715883
7	1.0733324587661446	3.345707139101757
8	0.8908659407759001	3.173642200519381
9	0.7871104697618394	3.154523874010228
>10	3.138901146080548	20.60159000748801
>50	0.06917031400937378	2.0291979330964787
>100	0.0381629318672407	3.33349265272091
>500	0.0047703664834050875	1.6510799199154547
>1k	0.0035777748625538154	3.0562769183381926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3735	1.983526375324613	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	1010	0.5363752715067897	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	1010	0.5363752715067897	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	939	0.4986696831137381	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	895	0.4753028396025512	No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	680	0.3611239451728881	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	595	0.3159834520262771	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	475	0.2522556969957674	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	448	0.23791695211390274	No Hit
ACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	410	0.21773649635424136	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	309	0.16409896920356237	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.15453980594898595	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	280	0.14869809507118922	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCG	275	0.14604277194491797	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.13011083318729058	No Hit
GACAGATTATTTTTATTTATTCAATGTCATATTTAAAATATTTATTTTTTA	228	0.12108273455796836	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	218	0.11577208830542589	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGC	213	0.11311676517915464	No Hit
AGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	193	0.10249547267406972	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38130440093254947	0.0
2	0.0	0.0	0.0	2.08442865412292	0.0
3	0.0	0.0	0.0	2.894302207635647	0.0
4	0.0	0.0	0.0	3.946341230264311	0.0
5	0.0	0.0	0.0	6.579890706900122	0.0
6	0.0	0.0	0.0	7.9643761849379455	0.0
7	0.0	0.0	0.0	9.460916298904413	0.0
8	0.0	0.0	0.0	11.82999559216361	0.0
9	0.0	0.0	0.0	12.663767053812778	0.0
10	0.0	0.0	0.0	14.777404262324682	0.0
11	0.0	0.0	0.0	18.571861009766277	0.0
12	0.0	0.0	0.0	21.13159250349175	0.0
13	0.0	0.0	0.0	22.166106393487023	0.0
14	0.0	0.0	0.0	22.532540984912455	0.0
15	0.0	0.0	0.0	23.325420470417043	0.0
16	0.0	0.0	0.0	24.912241570676734	0.0
17	0.0	0.0	0.0	26.921790112638806	0.0
18	0.0	0.0	0.0	29.22395526311597	0.0
19	0.0	0.0	0.0	30.58294963914159	0.0
20	0.0	0.0	0.0	31.83254470236483	0.0
21	0.0	0.0	0.0	33.28766177556147	0.0
22	0.0	0.0	0.0	34.64346976383556	0.0
23	0.0	0.0	0.0	35.98812539497931	0.0
24	0.0	0.0	0.0	36.910584649045944	0.0
25	0.0	0.0	0.0	37.65673044752816	0.0
26	0.0	0.0	0.0	38.197354236036986	0.0
27	0.0	0.0	0.0	38.71992182728716	0.0
28	0.0	0.0	0.0	39.22708854440497	0.0
29	0.0	0.0	0.0	39.691770091502434	0.0
30	0.0	0.0	0.0	40.26585095140228	0.0
31	0.0	0.0	0.0	40.71672481824313	0.0
32	0.0	0.0	0.0	41.11927180418585	0.0
33	0.0	0.0	0.0	41.48358213711027	0.0
34	0.0	0.0	0.0	41.870728248920614	0.0
35	0.0	0.0	0.0	42.25840542535621	0.0
36	0.0	0.0	0.0	42.58447910526232	0.0
37	0.0	0.0	0.0	42.96100392456758	0.0
38	0.0	0.0	0.0	43.29876102622928	0.0
39	0.0	0.0	0.0	43.60306105649997	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCAACG	25	3.878348E-5	45.000004	1
TGGGTAC	25	3.878348E-5	45.000004	6
GATGCTA	25	3.878348E-5	45.000004	9
CAATCTA	25	3.878348E-5	45.000004	10
GACTACT	25	3.878348E-5	45.000004	9
ACGAAGG	25	3.878348E-5	45.000004	2
GATCGAG	20	7.018252E-4	45.0	1
CCCTAGC	20	7.018252E-4	45.0	21
ACACAGG	20	7.018252E-4	45.0	2
GCCGGGA	20	7.018252E-4	45.0	4
ACGGGTA	20	7.018252E-4	45.0	5
TACGAAG	20	7.018252E-4	45.0	1
ACTACTC	20	7.018252E-4	45.0	10
CGGGCAA	20	7.018252E-4	45.0	6
AGATTCC	20	7.018252E-4	45.0	22
GATGCGG	20	7.018252E-4	45.0	2
CTATTAG	20	7.018252E-4	45.0	19
GGCGATA	35	1.2046985E-7	45.0	8
CTCTATT	20	7.018252E-4	45.0	17
AGTACGG	20	7.018252E-4	45.0	2
>>END_MODULE