SRR2934825.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934825.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208812
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4030	1.9299657107829054	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1488	0.7126027239813804	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1125	0.5387621401068904	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC	1075	0.5148171561021397	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	984	0.4712372852134935	No Hit
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	852	0.4080225274409517	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	611	0.29260770453805335	TruSeq Adapter, Index 14 (95% over 21bp)
TGGAGAGAAAGAAAGAAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAG	465	0.22268835124418135	No Hit
ACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	389	0.1862919755569603	TruSeq Adapter, Index 15 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	387	0.1853341761967703	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	377	0.18054517939582018	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	366	0.175277282914775	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	336	0.1609102925119246	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	330	0.15803689443135452	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	319	0.15276899795030938	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.13744420818726893	No Hit
GCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	262	0.1254717161848936	No Hit
ACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	261	0.12499281650479857	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	236	0.11302032450242322	No Hit
AGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	218	0.10440013026071299	TruSeq Adapter, Index 15 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	75	0.0	45.000004	1
TGATCGG	20	7.0200174E-4	45.0	2
CGTGGCG	25	3.8798105E-5	45.0	27
AAATCTA	20	7.0200174E-4	45.0	32
ATGATGG	50	2.1827873E-11	45.0	2
GGTAATT	35	1.2055534E-7	45.0	8
CTACTCA	40	6.7684596E-9	45.0	29
CCCTACA	35	1.2055534E-7	45.0	27
GCGCGAC	35	1.2055534E-7	45.0	9
AACTTGT	20	7.0200174E-4	45.0	45
GGGTAAT	50	2.1827873E-11	45.0	7
TAAGTAG	25	3.8798105E-5	45.0	1
CGCGACC	25	3.8798105E-5	45.0	10
GTGGCGA	25	3.8798105E-5	45.0	28
GTAATCA	20	7.0200174E-4	45.0	20
AGTATAG	20	7.0200174E-4	45.0	1
CGGGGTT	20	7.0200174E-4	45.0	6
ACGATGG	20	7.0200174E-4	45.0	2
AGTGCGG	20	7.0200174E-4	45.0	2
CTACGAA	40	6.7684596E-9	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content