##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934825.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208812
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679419765147596	31.0	31.0	34.0	30.0	34.0
2	31.795960002298717	33.0	31.0	34.0	30.0	34.0
3	31.80347872727621	33.0	31.0	34.0	30.0	34.0
4	35.58028753136793	37.0	35.0	37.0	33.0	37.0
5	32.98342528207191	37.0	35.0	37.0	28.0	37.0
6	34.028269448116006	37.0	35.0	37.0	27.0	37.0
7	35.36885811160278	37.0	35.0	37.0	32.0	37.0
8	35.31647127559719	37.0	35.0	37.0	33.0	37.0
9	37.396586403080285	39.0	37.0	39.0	34.0	39.0
10	36.80222879911116	39.0	37.0	39.0	32.0	39.0
11	36.639599256747694	39.0	37.0	39.0	32.0	39.0
12	36.94689481447426	39.0	37.0	39.0	33.0	39.0
13	36.8588874202632	39.0	37.0	39.0	33.0	39.0
14	37.82211271382871	40.0	37.0	41.0	33.0	41.0
15	37.98089669176101	40.0	37.0	41.0	33.0	41.0
16	37.94446200409938	40.0	37.0	41.0	33.0	41.0
17	37.91703063042354	39.0	37.0	41.0	33.0	41.0
18	37.84585177097102	39.0	37.0	40.0	33.0	41.0
19	37.644742639311914	39.0	36.0	40.0	33.0	41.0
20	37.4637233492328	39.0	35.0	40.0	33.0	41.0
21	37.369681819052545	39.0	35.0	40.0	33.0	41.0
22	37.337456659578955	39.0	35.0	40.0	33.0	41.0
23	37.10865754841676	39.0	35.0	40.0	32.0	41.0
24	36.810762791410454	39.0	35.0	40.0	32.0	41.0
25	36.583936746930256	38.0	35.0	40.0	31.0	41.0
26	36.89068157002471	39.0	35.0	40.0	32.0	41.0
27	36.78740206501542	39.0	35.0	40.0	32.0	41.0
28	36.83098672490087	39.0	35.0	40.0	32.0	41.0
29	36.90559450606287	39.0	35.0	40.0	32.0	41.0
30	36.5872076317453	38.0	35.0	40.0	31.0	41.0
31	36.43785318851407	38.0	35.0	40.0	31.0	41.0
32	36.11984943394058	38.0	35.0	40.0	30.0	41.0
33	35.897865065226135	38.0	35.0	40.0	30.0	41.0
34	35.80086393502289	38.0	35.0	40.0	29.0	41.0
35	35.55753979656342	38.0	35.0	40.0	27.0	41.0
36	35.406154818688584	38.0	35.0	40.0	26.0	41.0
37	35.39694078884355	38.0	35.0	40.0	25.0	41.0
38	35.25045974369289	38.0	35.0	40.0	25.0	41.0
39	35.18425186291976	38.0	35.0	40.0	25.0	41.0
40	35.05907227553972	38.0	35.0	40.0	24.0	41.0
41	34.85947167787292	38.0	35.0	40.0	23.0	41.0
42	34.96038062946574	38.0	35.0	40.0	23.0	41.0
43	34.93091872114629	38.0	35.0	40.0	23.0	41.0
44	34.8593615309465	38.0	35.0	40.0	23.0	41.0
45	34.854807194988794	38.0	35.0	40.0	23.0	41.0
46	34.68681397620826	38.0	34.0	40.0	23.0	41.0
47	34.6214968488401	38.0	34.0	40.0	23.0	41.0
48	34.600937685573626	38.0	34.0	40.0	23.0	41.0
49	34.619600406106926	38.0	34.0	40.0	22.0	41.0
50	34.55152002758462	38.0	34.0	40.0	22.0	41.0
51	33.575694883435816	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	4.0
12	10.0
13	5.0
14	15.0
15	15.0
16	29.0
17	65.0
18	107.0
19	188.0
20	316.0
21	488.0
22	818.0
23	1174.0
24	1704.0
25	2478.0
26	3499.0
27	3983.0
28	3760.0
29	3734.0
30	4118.0
31	4903.0
32	6161.0
33	8814.0
34	13470.0
35	18971.0
36	22833.0
37	30044.0
38	43183.0
39	33912.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.74842442005249	22.65961726337567	23.640403808210255	17.95155450836159
2	36.166503840775434	22.837289044690916	25.456870294810642	15.539336819723005
3	26.93714920598433	23.642319406930635	35.212056778346074	14.20847460873896
4	24.821849319004656	24.32666704978641	34.610079880466635	16.241403750742293
5	18.78244545332644	33.27778097044231	32.13177403597494	15.807999540256306
6	20.502174204547632	32.842940060916035	33.25479378579775	13.400091948738579
7	66.64894737850315	7.256766852479743	20.366166695400647	5.728119073616459
8	64.46755933567036	7.4904698963661085	20.49642740838649	7.545543359577035
9	59.16566097733846	9.411815412907304	23.59299273988085	7.829530869873379
10	30.003543857632703	23.771143420876196	31.351167557420073	14.874145164071031
11	21.360841330957992	24.68584180985767	34.33806486217267	19.615251997011665
12	20.08170028542421	21.83543091393215	39.9440645173649	18.138804283278738
13	19.536233549795988	25.1345708101067	38.895274217956825	16.43392142214049
14	15.218474034059346	27.441430569124382	37.846005018868645	19.49409037794763
15	14.264984770990171	25.593835603317817	41.85631093998429	18.284868685707718
16	14.024577131582477	26.37252648315231	38.30574871175986	21.297147673505354
17	13.759745608489935	27.181866942512883	35.267609141237095	23.790778307760093
18	15.0211673658602	25.60724479436048	40.126046395801005	19.245541443978315
19	16.048407179664004	27.746489665344903	36.30203245024232	19.903070704748767
20	17.41853916441584	28.012278987797636	36.6693485048752	17.89983334291133
21	16.107311840315692	28.303449993295406	37.46958987031397	18.11964829607494
22	13.720954734402238	26.52050648430167	35.26856694059728	24.48997184069881
23	14.201291113537536	26.666570886730646	35.51759477424669	23.614543225485125
24	17.226979292377834	24.330019347547076	36.89299465547957	21.55000670459552
25	13.701798747198438	27.917935750818916	33.95733961649714	24.42292588548551
26	13.47958929563435	28.190429668792984	34.90747658180564	23.422504453767022
27	15.725628795279963	27.50033522977607	34.58661379614198	22.187422178801985
28	12.3608796429324	28.30297109361531	34.84905082083405	24.48709844261824
29	15.895638181713695	25.25716912821102	33.15614045169818	25.691052238377104
30	17.600042143171848	29.21096488707546	30.32632224201674	22.862670727735953
31	17.72264046127617	31.76062678390131	27.68614830565293	22.83058444916959
32	17.589985249889853	29.357029289504432	29.271306246767427	23.781679213838284
33	17.86008466946344	28.915004884776735	29.745895829741585	23.479014616018237
34	17.69486427983066	26.562170756469932	30.74535946209988	24.997605501599523
35	17.329463823918164	25.09051203953796	30.119437580215696	27.46058655632818
36	17.49324751451066	28.438499703082197	30.30908185353332	23.759170928873818
37	19.03195218665594	29.197555696032794	31.107886519931803	20.66260559737946
38	18.577955289925864	31.012106583912804	29.857479455203723	20.552458670957606
39	21.62615175373063	27.081776909373023	31.416297913912995	19.875773422983354
40	22.548512537593624	24.564680190793634	32.40809915138977	20.478708120222976
41	17.795912112330708	24.878359481255867	31.83054613719518	25.49518226921824
42	18.53341761967703	23.740493841350112	34.34764285577457	23.378445683198283
43	20.864701262379555	23.774016818956763	31.850659923759174	23.510621994904508
44	19.516598662912095	26.364385188590695	28.726318410819303	25.39269773767791
45	22.272187422178803	23.983295979158285	28.001264295155455	25.743252303507465
46	22.099304637664503	25.302185698139958	30.696511694730187	21.901997969465356
47	17.31557573319541	24.97318161791468	35.56069574545524	22.150546903434666
48	17.969273796525105	23.15240503419344	35.28437063004042	23.59395053924104
49	20.58885504664483	21.216213627569296	36.56159607685382	21.633335248932053
50	19.964848763481026	20.650154205696992	34.38451813114189	25.000478899680097
51	18.808784935731662	20.49786410742678	31.4637089822424	29.22964197459916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1514.0
1	2061.5
2	2609.0
3	4724.0
4	6839.0
5	4503.0
6	2167.0
7	2210.5
8	2254.0
9	2476.0
10	2698.0
11	2617.0
12	2536.0
13	2599.0
14	2662.0
15	2441.5
16	2221.0
17	2168.5
18	2116.0
19	2012.5
20	1909.0
21	1716.5
22	1524.0
23	1579.0
24	1634.0
25	1516.5
26	1834.0
27	2269.0
28	2477.5
29	2686.0
30	2737.5
31	2789.0
32	3352.0
33	3915.0
34	3878.5
35	3842.0
36	4183.0
37	4524.0
38	4762.5
39	5001.0
40	5614.0
41	6227.0
42	8066.0
43	9905.0
44	11557.0
45	13209.0
46	16659.0
47	20109.0
48	23489.0
49	26869.0
50	26510.5
51	26152.0
52	20680.0
53	15208.0
54	12348.0
55	9488.0
56	7837.5
57	6187.0
58	5312.5
59	4438.0
60	4079.0
61	3720.0
62	3327.5
63	2935.0
64	2447.5
65	1960.0
66	1661.5
67	1363.0
68	1094.5
69	826.0
70	642.0
71	458.0
72	386.0
73	314.0
74	242.5
75	120.5
76	70.0
77	55.0
78	40.0
79	27.5
80	15.0
81	18.0
82	21.0
83	13.0
84	5.0
85	8.5
86	12.0
87	6.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	208812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.37915445472483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28589927591752	32.96745397774074
2	9.951547982604755	8.832825699672432
3	4.305646980112011	5.732429170737314
4	2.4118097745740212	4.2813631400494225
5	1.7039139302247783	3.7809129743501333
6	1.3143553939289299	3.49979886213436
7	1.055369109410914	3.278547209930464
8	0.8503383008341516	3.0189835833189664
9	0.7586139917340211	3.0299982759611517
>10	3.253515199257573	21.14677317395552
>50	0.06366746161067886	2.066931019290079
>100	0.037768833158877295	3.4959676646936
>500	0.003237328556475197	1.1718675171924986
>1k	0.004316438075300262	3.6961477309733155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4030	1.9299657107829054	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1488	0.7126027239813804	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1125	0.5387621401068904	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCC	1075	0.5148171561021397	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	984	0.4712372852134935	No Hit
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	852	0.4080225274409517	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	611	0.29260770453805335	TruSeq Adapter, Index 14 (95% over 21bp)
TGGAGAGAAAGAAAGAAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAG	465	0.22268835124418135	No Hit
ACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	389	0.1862919755569603	TruSeq Adapter, Index 15 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	387	0.1853341761967703	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	377	0.18054517939582018	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	366	0.175277282914775	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	336	0.1609102925119246	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	330	0.15803689443135452	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	319	0.15276899795030938	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.13744420818726893	No Hit
GCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	262	0.1254717161848936	No Hit
ACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	261	0.12499281650479857	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	236	0.11302032450242322	No Hit
AGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	218	0.10440013026071299	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4401088060073176	0.0
2	0.0	0.0	0.0	2.3260157462214814	0.0
3	0.0	0.0	0.0	3.15307549374557	0.0
4	0.0	0.0	0.0	4.232036472999636	0.0
5	0.0	0.0	0.0	6.888492998486677	0.0
6	0.0	0.0	0.0	8.36063061509875	0.0
7	0.0	0.0	0.0	9.939562860372009	0.0
8	0.0	0.0	0.0	12.373809934294965	0.0
9	0.0	0.0	0.0	13.26839453671245	0.0
10	0.0	0.0	0.0	15.687316820872363	0.0
11	0.0	0.0	0.0	19.530965653314944	0.0
12	0.0	0.0	0.0	22.109840430626594	0.0
13	0.0	0.0	0.0	23.134206846349826	0.0
14	0.0	0.0	0.0	23.51397429266517	0.0
15	0.0	0.0	0.0	24.310863360343276	0.0
16	0.0	0.0	0.0	26.125414248223283	0.0
17	0.0	0.0	0.0	28.271363714729038	0.0
18	4.788996800950137E-4	0.0	0.0	30.494894929410187	0.0
19	4.788996800950137E-4	0.0	0.0	31.8477865256786	0.0
20	4.788996800950137E-4	0.0	0.0	33.09867249008678	0.0
21	4.788996800950137E-4	0.0	0.0	34.61247437886711	0.0
22	4.788996800950137E-4	0.0	0.0	36.166503840775434	0.0
23	4.788996800950137E-4	0.0	0.0	37.557228511771356	0.0
24	4.788996800950137E-4	0.0	0.0	38.579200429094115	0.0
25	4.788996800950137E-4	0.0	0.0	39.320537133881196	0.0
26	4.788996800950137E-4	0.0	0.0	39.928739727601865	0.0
27	4.788996800950137E-4	0.0	0.0	40.46462846962818	0.0
28	4.788996800950137E-4	0.0	0.0	40.94975384556443	0.0
29	4.788996800950137E-4	0.0	0.0	41.53257475624006	0.0
30	4.788996800950137E-4	0.0	0.0	42.091450682910946	0.0
31	4.788996800950137E-4	0.0	0.0	42.53586958603912	0.0
32	4.788996800950137E-4	0.0	0.0	42.91132693523361	0.0
33	4.788996800950137E-4	0.0	0.0	43.27481179242572	0.0
34	4.788996800950137E-4	0.0	0.0	43.59567457808938	0.0
35	4.788996800950137E-4	0.0	0.0	43.96059613432178	0.0
36	4.788996800950137E-4	0.0	0.0	44.23835794877689	0.0
37	4.788996800950137E-4	0.0	0.0	44.588433614926345	0.0
38	4.788996800950137E-4	0.0	0.0	44.87529452330326	0.0
39	4.788996800950137E-4	0.0	0.0	45.160718732639886	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	75	0.0	45.000004	1
TGATCGG	20	7.0200174E-4	45.0	2
CGTGGCG	25	3.8798105E-5	45.0	27
AAATCTA	20	7.0200174E-4	45.0	32
ATGATGG	50	2.1827873E-11	45.0	2
GGTAATT	35	1.2055534E-7	45.0	8
CTACTCA	40	6.7684596E-9	45.0	29
CCCTACA	35	1.2055534E-7	45.0	27
GCGCGAC	35	1.2055534E-7	45.0	9
AACTTGT	20	7.0200174E-4	45.0	45
GGGTAAT	50	2.1827873E-11	45.0	7
TAAGTAG	25	3.8798105E-5	45.0	1
CGCGACC	25	3.8798105E-5	45.0	10
GTGGCGA	25	3.8798105E-5	45.0	28
GTAATCA	20	7.0200174E-4	45.0	20
AGTATAG	20	7.0200174E-4	45.0	1
CGGGGTT	20	7.0200174E-4	45.0	6
ACGATGG	20	7.0200174E-4	45.0	2
AGTGCGG	20	7.0200174E-4	45.0	2
CTACGAA	40	6.7684596E-9	45.0	11
>>END_MODULE